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CaWRKY2, a Chili Pepper Transcription Factor, Is Rapidly Induced by Incompatible Plant Pathogens

  • Oh, Sang-Keun;Yi, So Young;Yu, Seung Hun;Moon, Jae Sun;Park, Jeong Mee;Choi, Doil
    • Molecules and Cells
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    • v.22 no.1
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    • pp.58-64
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    • 2006
  • WRKY family proteins are a class of plant-specific transcription factors involved in stress response signaling pathways. In this study a gene encoding a putative WRKY protein was isolated from a pepper EST database (http://genepool.kribb.re.kr). The cDNA, named Capsicum annuum WRKY2 (CaWRKY2), encodes a putative polypeptide of 548 amino acids, containing two WRKY domains with zinc finger motifs and two potential nuclear localization signals. Northern blot analyses showed that CaWRKY2 mRNA was preferentially induced during incompatible interactions of pepper plants with PMMoV, Pseudomonas syringae pv. syringae 61, and Xanthomonas axonopodis pv. vesicatoria race 3. Furthermore, CaWRKY2 transcripts were strongly induced by wounding and ethephon treatment, whereas only moderate expression was detected following treatment with salicylic acid and jasmonic acid. CaWRKY2 was translocated to the nucleus when a CaWRKY2-smGFP fusion construct was expressed in onion epidermal cells. CaWRKY2 also had transcriptional activation activity in yeast. Taken together our data suggest that CaWRKY2 is a pathogen-inducible transcription factor that may have a role in early defense responses to biotic and abiotic stresses.

Magnetocapacitance Properties of Multilayered CoFe2O4/BaTiO3/CoFe2O4 Thin Film by Pulsed Laser Deposition

  • Lee, Seong Noh;Shim, Hyun Ju;Shim, In-Bo
    • Journal of Magnetics
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    • v.19 no.2
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    • pp.121-125
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    • 2014
  • $CoFe_2O_4(CFO)/BaTiO_3(BTO)/CoFe_2O_4(CFO)$ multilayered thin films were deposited on $Pt/TiO_2/SiO_2/Si$ substrates by the pulsed laser deposition (PLD) system with KrF excimer laser (${\lambda}=248nm$). BTO, CFO, BTO/CFO and CFO/BTO/CFO structured thin films were prepared and their crystal structures and microstructures, as well as their magnetic and magneto-electrical properties, were studied. The C-V characteristics of these multilayered thin films with different capacitor structures were obtained to confirm the change in their capacitances under a magnetic field. Finally, the capacitance of the CFO/BTO/CFO thin film as a function of bias voltage under an in-plane magnetic field of 1,000 Oe increased to 951.04 pF at 1 MHz, from 831.90 pF measured under no magnetic field, indicating 14.3% increase in magnetocapacitance.

Growth of MnS Thin Film on c-Sapphire by Pulsed Laser Deposition (PLD 법에 의한 c-사파이어 기판위의 MnS 박막성장)

  • Song, Jeong-Hwan
    • Korean Journal of Materials Research
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    • v.17 no.9
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    • pp.475-479
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    • 2007
  • Pulsed laser deposition was utilized to grow MnS thin films on c-sapphire substrate using a KrF excimer laser at growth temperatures that ranged from room temperature to $700^{\circ}C$. The results of X-ray diffraction (XRD) and UV-visible spectroscopy were employed to investigate the structural and optical properties of the MnS films. While the growth rate decreased as $T_s$ increased, the overall quality of the film improved. The highest quality MnS film was obtained at $700^{\circ}C$. Variations in the $T_s$ resulted in the MnS films exhibiting different growth mechanisms. The oriented (200) rocksalt MnS film was grown at room temperature. In the case of higher $T_s,\;200{\sim}500^{\circ}C$, the films consisted of mixed phases of rocksalt and wurtzite. The main structure of the films was altered to (111) rocksalt when the temperature was increased to in excess of $600^{\circ}C$. This behavior may very well be the result of elements such as surface energy and atomic arrangement during the growth process. The optical band gap of the obtained ${\alpha}-MnS$ film was estimated to be 3.32 eV.

Thin film transistor with pulsed laser deposited ZnO active channel layer (펄스 레이저 증착법으로 제작한 ZnO를 채널층으로 한 박막트랜지스터)

  • Shin, P.K.;Kim, C.J.;Song, J.H.;Kim, S.J.;Kim, J.T.;Cho, J.S.;Lee, B.S.;Ebihara, Kenji
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.1884-1886
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    • 2005
  • KrF 펄스 레이저 증착법(pulsed laser deposition: PLD)으로 ZnO 박막을 증착하여 평판 디스플레이 소자 구동용 박막 트랜지스터(thin film transistor) 소자를 제작하였다. 전도성이 높은 실리콘웨이퍼(c-Si, 하부전극) 기판 위에 LPCVD 법으로 silicon nitride 박막을 절연막으로 형성하고, 다양한 공정 조건에서 펄스 레이저 증착법으로 제작한 ZnO 박막을 증착하여 채널층으로 하였으며, Al 박막을 증착하고 패터닝하여 소스 및 드레인 전극으로 하였다. ZnO 박막의 증착 시에 기판 온도를 다양하게 조절하고 산소 분압을 변화시켜 ZnO 박막의 특성을 조절하였다. 제작된 박막의 표면특성은 AFM(atomic force microscopy)로 분석하고, 결정특성은 XRD(X-ray diffraction)로 조사하였다. ZnO 박막의 전기적 특성은 Hall-van der Pauw 법으로 측정하였고, 광학 투과도(optical transparency)를 UV-visible photometer로 조사하였다. ZnO-TFT 소자는 $10^6$ 수준의 on-off ratio와 $2.4{\sim}6.1cm^2/V{\cdot}s$의 전계효과이동도(field effect mobility)를 보였다.

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Study on the inactivation of intracellular enzyme mlecules by X-ray irradiation (X-선조사(線照射)에 의한 세포내효소분자(細胞內酵素分子)의 불활성화(不活性化)에 관한 연구(硏究))

  • Lee, Sang-Bok
    • Journal of Radiation Protection and Research
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    • v.2 no.1
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    • pp.31-37
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    • 1977
  • Inactivation of the glutamic acid dehydrogenase and glucose-6-phosphate dehydrogenase enzyme-molecules in the Ehrlich ascites tumor cells of the mouse were studied. The above mentioned intracellular enzymemolecules were irradiated by the X-ray radiation under the condition of 65 kV, I Amp. under the atmosphere of nitrogen gases and by $4^{\circ}C$. Thereby, irradiation doses were 580 KR/min($error:{\pm}3%$). After irradiation, the cell homogentes were prepared through liquid air techniquese. There after, the activities of the enzymes were measured with photometric method given by O. Warburg and W. Christian. The dose effect curves of the activities of the two enzymes by the X-ray irradiation showed both exponential and the inactivation doses were $6,5.10^{0}\;and\;5,0.10^{6}$ R respectively. These results showed one side that the inactivation process of the intracelluar enzymemolecules was one hit reaction after target theory, and the other side that this inactivation process could not be the primary causes of the death through X-ray irradiation of the vertebrate animals, because of the high resistance of the intracellular protein molecules against X-ray irradiation. The one hit reaction by the inactivation process of the irradiated intracellular enzymemolecules was discussed.

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Gender Differences in Influence of Socio-demographic Characteristics on Mode Choice in India

  • SAIGAL, Taru;VAISH, Arun Kr.;RAO, N.V.M.
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.1
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    • pp.531-542
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    • 2021
  • The study aims to investigate differences between men and women in influence of various socio-demographic factors on choice of mode of transport. For this purpose, a binary logit model of choice probabilities is implemented on survey data of a developing country city. Results indicate women's choice of travel mode to be more environment-friendly than that of men. Well-educated, working and middle-aged individuals appear to be the most likely to choosing more-polluting modes of transport for frequent travelling purposes. Individuals in the sample who are the least socioeconomically well off are found the most likely to be promising for the environment. The findings of this study suggest the future transportation policies toward development of existing infrastructure of greener modes of transportation in the city such as, public transportation services and pedestrian lanes, so as to manage the rising issues of degrading environmental quality. The study highlights how the consideration and inclusion of socio-demographic factors is crucial for policy recommendation regarding curtailing the environmental damages contributed by transportation sector. Because mobility crucially affects all other indicators of empowerment, and women are the ones using green modes extensively, the city's transportation system should be so developed which gives their safety and security due importance.

Q-omics: Smart Software for Assisting Oncology and Cancer Research

  • Lee, Jieun;Kim, Youngju;Jin, Seonghee;Yoo, Heeseung;Jeong, Sumin;Jeong, Euna;Yoon, Sukjoon
    • Molecules and Cells
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    • v.44 no.11
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    • pp.843-850
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    • 2021
  • The rapid increase in collateral omics and phenotypic data has enabled data-driven studies for the fast discovery of cancer targets and biomarkers. Thus, it is necessary to develop convenient tools for general oncologists and cancer scientists to carry out customized data mining without computational expertise. For this purpose, we developed innovative software that enables user-driven analyses assisted by knowledge-based smart systems. Publicly available data on mutations, gene expression, patient survival, immune score, drug screening and RNAi screening were integrated from the TCGA, GDSC, CCLE, NCI, and DepMap databases. The optimal selection of samples and other filtering options were guided by the smart function of the software for data mining and visualization on Kaplan-Meier plots, box plots and scatter plots of publication quality. We implemented unique algorithms for both data mining and visualization, thus simplifying and accelerating user-driven discovery activities on large multiomics datasets. The present Q-omics software program (v0.95) is available at http://qomics.sookmyung.ac.kr.

Am Experimental Study on Measurement of Number Density and Temperature Distributions in $C_3H_8/O_2$ Flame by UV Laser Rayleigh Scattering (UV Laser Rayleigh Scattering을 이용한 $C_3H_8/O_2$ 화염에서 가스 성분의 농도 및 온도 분포 계측에 관한 실험적 연구)

  • Jin, S. H;Nam, G. J.;Kim, H. S.;Chang, N. K.;Park, S. H.;Kim, U.;Park, K. S.;Kim, G. S.
    • Transactions of the Korean Society of Automotive Engineers
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    • v.5 no.2
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    • pp.60-68
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    • 1997
  • Rayleigh Scattering Cross Sections($\sigma$i) of various gases and the temperature distributions of premixes C3H8/O2 flame are measured by high power KrF(248nm) Exci- mer laser and ICCD camera. Results show that $\sigma$i of O2 and Propane(C3H8) gases agree well in the 5% error range, but of H2 has the more or less difference from the calcul- ated value by other groups. This is attributed to the low RS signal of H2 to Nosie level(S/N ratio). The temperature distributions of flame range out between 300K in the air and about 2000K in the burned area. In this temperature range, out system has the about 250K temperature resolution. Because low RS signals in the reaction area with high temperature are affected highly by noises, temperature uncertainty of this area is relatively high to another part of flame. Experimental results show that UV Rayleigh Scattering can be used for the measurement of mixing ratio of mixed gases and the temperature distributions of flame. Especially, this technique can be applied for the measurement of the mixing ratio of air/fuel before the ignition and the flame structure after the ignition inside the Engine.

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Separation Characteristics of Ibuprofen in Kromasil HPLC Column (Kromasil HPLC 칼럼을 이용한 Ibuprofen의 분리특성 연구)

  • Park Joon Sub;Kim Byung Lip;Yoon Tae Ho;Kim In Ho
    • KSBB Journal
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    • v.20 no.3
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    • pp.244-249
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    • 2005
  • Chiral separation of racemic ibuprofen was achieved on a Kromasil KR100-5CHI-TBB column. Some chromatographic parameters (resolution, number of theoretical plates, HETP, capacity factor) are calculated under different separation conditions such as change of mobile phase compositions (hexane / t-BME : 85 / 15, 75 / 15, 65 / 35, 55 /45) as well as acetic acid concentrations for adjusting pH (0.1 to 1 $v/v\%$). Flow rate versus number of theoretical plates and HETP were compared to evaluate column efficiency. To determine the adsorption isotherms, PIM (Pulsed Input Method) was carried out. At concentrations of racemic ibuprofen between 0.1 and 0.3 mg/ml, S- and R-ibuprofen have the same retention time of 4.48 and 5.81 min. Ibuprofen isotherms show a linear form under concentrations of 0.3 mg/ml with eluent (hexane / t-BME = 55 / 45).

일본의 과학 및 학술 정보 정책의 수립 과정과 실시 체제의 분석

  • 현규섭
    • Journal of the Korean Society for information Management
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    • v.5 no.1
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    • pp.31-52
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    • 1988
  • The information policy in Japan have bccir growing rapldlr 111 recent vttars. \'arioi~s level of govc~riimerital organrzatlons, ir~bt~tution arid agrircics have undt~rtak~~rr iriforrnatiori polrcy 111 order to i r l - crease information flow toward tlie society in Japan. At tlie same trme, computerized library systems also h a w uridergoirc rapid expansion. NACSIS(National Ceriter for- Science Informat~on System) wl~icl~ was cstablisl~ed bv Mirristr> of Etlr~c~arion, Sc~ence arid Culture haw witnessed dramatic adv;rnces i r i promoting for corrstructiori of database so as to provide their 11rf'3r.mat1orr nu nationwide scale. T l r i h articlr inrroduccs tlie actr\rtle> and ~mplcmeritat~o~~ of it format in^^ pol~cy wl~i(~li taktas a place hy goverrirnrlit of Japar~ aid arraly~c,.; thi~ ~ I . O I . P S I of deciiioii maltiiig iri polit~cal procedures. So far as b:ast-A\~ari riatioris art, ~ . n r i v t ~ r ~ ~ , e.;pc,ciall\. Kr,rc.an a~rd Japair !rave ,imilar cultural and liistorical backjir. ourii1, ard could t)t. tliif most a p p r o p r i a t ~ ~ caac stlldh for political ~nq~lerncritat~orr. Thus the topic of this artrclc will be useful to r t ~ a l l ~ t . Ore, trail ur~d c r r o r prorex, iri iiiior.matior~ pnlic\ulcorner.

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