• Journal of Applied Biological Chemistry
• /
• v.57 no.3
• /
• pp.267-277
• /
• 2014

Objective of this paper is to assess the available data on the pesticide uses and regulations in the golf courses, and provide the nationwide systematic management options. Numbers of golf courses in Korea are rapidly increasing from 2000s and reached at 421 sites by the end of 2011. Accordingly pesticide usage has been increased with years in direct proportion to the increasing number of golf courses. Amounts of pesticide applied in 2011 were 118,669 kg as of an active ingredient and were in the orders of fungicides (54.9%) > insecticides (24.4%) > herbicides (13.3%) > growth regulators (0.1%). Average pesticide usages in 2011 were 280.9 kg per golf course and $5.4kg\;ha^{-1}$. Frequencies of the residual pesticide detections in green and turf were higher than those in fairway and soil, respectively. Residue of highly toxic pesticides was not detected in golf courses. Ministry of Environment in 2010 has developed the 'golf course pesticide monitoring and management system' which is the advanced online registry for kind and amount of pesticides applied in each golf course. This system is intended for monitoring of the pesticide uses and residual levels and protecting the environmental pollution from pesticides in the golf course. In 2009, management of pesticides in the golf courses became the task of Ministry of Environment, being merged from many federal agency and ministries. The protocol for the site-specific best management practices, on which to base results from the risk assessment, should be set for pesticides in the golf to minimize the environmental impacts.

• Journal of Life Science
• /
• v.19 no.9
• /
• pp.1226-1231
• /
• 2009

Enzyme substitution with recombinant phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is currently being explored for treatment of phenylketonuria (PKU), an autosomal recessive genetic disorder with mutations of the gene encoding phenylalanine-4-hydroxylase (EC 1.14.16.1). However, oral administration of PAL is limited because of proteolytic digestion in the gastrointestinal tract. The aim of this study was to determine the biochemical properties of PAL and delinate the susceptibility of wild-type PAL to pancreatic proteolysis by exploring several mutants, and to develop therapeutic drugs with PAL for PKU. The specific activity of PAL was assayed and its optimal pH, temperature stability, and intestinal protease susceptibility were investigated. Its $V_{max}$ values for phenylalanine and tyrosine were 1.77 and $0.47{\mu}mol$/ min/mg protein, respectively, and its $K_m$ values were $4.77{\times}10^{-4}$ and $4.37{\times}10^{-4}\;M$, respectively. PAL showed an optimal pH at 8.5, corresponding to the average pH range of the small intestine. It showed no loss of activity at $-80^{\circ}C$ for 5 months and possessed 93.4% of its activity under $4^{\circ}C$ for 4 wks. PAL was susceptible to chymotrypsin digestion and, to a lesser extent, to trypsin, elastase, carboxypeptidase A, and B. The trypsin and chymotrypsin cleaving sites were mutated to investigate protection from pancreatic digestion and the specific activities of these mutants were evaluated. The six mutants displayed low specific activities compared to the wild-type, suggesting that the primary trypsin and chymotrypsin cleaving sites may be essential for catalytic reaction. The PAL mutants could therefore be applied as a pretreatment modality without susceptibility to proteolytic attack, however, additional modification for enhancing enzymatic activity is needed to reduce the Phe levels effectively.

• Atmosphere
• /
• v.23 no.1
• /
• pp.85-92
• /
• 2013

We estimate atmospheric radiative heating effect of aerosols, based on AErosol RObotic NETwork (AERONET) and lidar observations and radiative transfer calculations. The column radiation model (CRM) is modified to ingest the AERONET measured variables (aerosol optical depth, single scattering albedo, and asymmetric parameter) and subsequently calculate the optical parameters at the 19 bands from the data obtained at four wavelengths. The aerosol radiative forcing at the surface and the top of the atmosphere, and atmospheric absorption on pollution (April 15, 2001) and dust (April 17~18, 2001) days are 3~4 times greater than those on clear-sky days (April 14 and 16, 2001). The atmospheric radiative heating rate (${\Delta}H$) and heating rate by aerosols (${\Delta}H_{aerosol}$) are estimated to be about $3\;K\;day^{-1}$ and $1{\sim}3\;K\;day^{-1}$ for pollution and dust aerosol layers. The sensitivity test showed that a 10% uncertainty in the single scattering albedo results in 30% uncertainties in aerosol radiative forcing at the surface and at the top of the atmosphere and 60% uncertainties in atmospheric forcing, thereby translated to about 35% uncertainties in ${\Delta}H$. This result suggests that atmospheric radiative heating is largely determined by the amount of light-absorbing aerosols.

• Journal of the Korean earth science society
• /
• v.36 no.1
• /
• pp.90-108
• /
• 2015

The WRF-Chem model was applied to simulate the Asian dust event affecting the Korean Peninsula from 11 to 13 November 2010. GOCART dust emission schemes, RADM2 chemical mechanism, and MADE/SORGAM aerosol scheme were adopted within the WRF-Chem model to predict dust aerosol concentrations. The results in the model simulations were identified by comparing with the weather maps, satellite images, monitoring data of $PM_{10}$ concentration, and LIDAR images. The model results showed a good agreement with the long-range transport from the dust source area such as Northeastern China and Mongolia to the Korean Peninsula. Comparison of the time series of $PM_{10}$ concentration measured at Backnungdo showed that the correlation coefficient was 0.736, and the root mean square error was $192.73{\mu}g/m^3$. The spatial distribution of $PM_{10}$ concentration using the WRF-Chem model was similar to that of the $PM_{2.5}$ which were about a half of $PM_{10}$. Also, they were much alike in those of the UM-ADAM model simulated by the Korean Meteorological Administration. Meanwhile, the spatial distributions of $PM_{10}$ concentrations during the Asian dust events had relevance to those of both the wind speed of u component ($ms^{-1}$) and the PBL height (m). We performed a regressive analysis between $PM_{10}$ concentrations and two meteorological variables (u component and PBL) in the strong dust event in autumn (CASE 1, on 11 to 23 March 2010) and the weak dust event in spring (CASE 2, on 19 to 20 March 2011), respectively.

• Economic and Environmental Geology
• /
• v.31 no.6
• /
• pp.463-472
• /
• 1998

The survey was carried out in order to delineate the occurrence of ore deposits and the mineralized characteristics in the Estancia de la Virgen area through the 1:2,000 scaled geological mapping and topographic measuring surveys. Gold-silver mineralization is in the fault block developed between the San Agustin Fault and Cabanas Fault. It is associated with ore bearing quartz veins controlled by the fault structure. The contents of Au and Ag range from traces up to 72 g/t and 180 g/t respectively. According to traversing the outcrops, the quartz veins are traced by 0.5 Km trended to north and south. In those extended part, they continue for 1,000 m intermittently. Gold-silver mineralization could be divided into three stages. In the first stage, pyrite, galena, sphalerite, and chalcopyrite were formed with the primary silver and gold associated with galena and copper sulfides respectively. In the second stage, Cu-Bi-Au-Ag bearing sulfides such as chalcocite, covellite, and linarite are formed and usually deposited on the cataclastic fractures of galena and/or chalcopyrite. In the third stage, both the carbonation of galena and sphalerite and the sulphatization of galena, took place in the surface environment. And then primary silver was carried away off and was deposited on galena and/or copper sulfides during oxidation near the water table. Low partitionings of Fe in sphalerite assist that the minerals were formed at the relatively low temperature, which is coincided with previously reported homogenization temperature of fluid inclusions.

• International Journal of Highway Engineering
• /
• v.17 no.2
• /
• pp.107-122
• /
• 2015

PURPOSES : The use of virtual driving tests to determine actual road driving behavior is increasing. However, the results indicate a gap between real and virtual driving under same road conditions road based on ergonomic factors, such as anxiety and speed. In the future, the use of virtual driving tests is expected to increase. For this reason, the purpose of this study is to analyze the gap between real and virtual driving on same road conditions and to use a calibration formula to allow for higher reliability of virtual driving tests. METHODS : An intelligent driving recorder was used to capture real driving. A driving simulator was used to record virtual driving. Additionally, a virtual driving map was made with the UC-Win/Road software. We gathered data including geometric structure information, driving information, driver information, and road operation information for real driving and virtual driving on the same road conditions. In this study we investigated a range of gaps, driving speeds, and lateral positions, and introduced a calibration formula to the virtual record to achieve the same record as the real driving situation by applying the effects of the main causes of discrepancy between the two (driving speed and lateral position) using a linear regression model. RESULTS: In the virtual driving test, driving speed and lateral position were determined to be higher and bigger than in the real Driving test, respectively. Additionally, the virtual driving test reduces the concentration, anxiety, and reality when compared to the real driving test. The formula includes four variables to produce the calibration: tangent driving speed, curve driving speed, tangent lateral position, and curve lateral position. However, the tangent lateral position was excluded because it was not statistically significant. CONCLUSIONS: The results of analyzing the formula from MPB (mean prediction bias), MAD (mean absolute deviation) is after applying the formula to the virtual driving test, similar to the real driving test so that the formula works. Because this study was conducted on a national, two-way road, the road speed limit was 80 km/h, and the lane width was 3.0-3.5 m. It works in the same condition road restrictively.

• The Korean Journal of Emergency Medical Services
• /
• v.8 no.1
• /
• pp.117-126
• /
• 2004

In general, the patients who transferred to the hospital by 119 rescue service at night go to the emergency room(ER) of general hospital rather than that of their nearby the 1st(clinic) and 2nd(local hospital) hospital. And the hospital is mainly selected not by 119 EMST but by patients or his/her conservators. Therefore we had studied retrospectively with emergency situation diary and medical chart for 697 patients of being transferred to one emergency medical center for 6 months since January 2004, and results are as follows. 1. The 280 patients(42.5%) of being transferred hospital at night by 119 rescue service were not emergency case and their average staying time in hospital was about $7.15{\pm}10.06$ minutes. 2. Transfer time was distributed in each time intervals of 1819, 2021, 2223, and 2401 and patients ratio in each intervals were 15.1%, 17.8%, 16.4%, and 15.2%. 3. In response of ambulance, the average time from the spot to the hospital was $14.53{\pm}9.27min$. and average distance of that was $7.95{\pm}9.21km$. 4. Diseases rather than traffic accidents or traumatic injury were main causes of ambulance calling and its value was 533(76.5%), and accidents were mainly occurred in patients' house and its value was 479(68.7%). 5. In time of transfer by 119 rescue service, hospital was mainly selected by patient/conservator and its value was 648(93.0%). In result. the hospital was selected not by EMST but by patient/conservator. 6. The case that the 1st grade EMT was rode in ambulance was 161(23.1%), and the case that 2nd EMT and emergency team member who educated for emergency were rode in ambulance were 504(72.3%). So the number of the 1st grade EMT was short in fire station of Kwangju metropolitan city than other city. 7. The first aids for patients before reaching hospital were limited to oxygen inhalation, airway control, and BLS for maintaining limbs and spine. So it seems to be a simple patients transfer. Consequently, to establish an efficient emergency medical system, it has been thought that it should be advanced a moderate education and public information about the appropriate use of emergency medical system toward citizen, and also need the hospital selection by the patients categorizing standards for 119 rescue service member, securing the 1st grade EMT, appropriate first-aids education, and securing professional human power in emergency room of the Ist(clinic) and 2nd(local hospital) hospital at night.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.3
• /
• pp.424-431
• /
• 2007

The aim of this study was to determine the effects of short road transportation in an open truck during hot season on live weight shrink, physiological responses, and carcass and meat quality of Omani sheep at 6 and 12 months of age. Thirty-six male sheep, 18 of each age group, were used. Age groups were assigned randomly to transported and not-transported groups. The transported group was transported to the slaughterhouse the day of slaughter in an open truck covering a distance of approximately 100 km. The average temperature during transportation was $37^{\circ}C$. The not-transported group was kept in a lairage of a commercial slaughterhouse with ad libitum feed and water for 48 h prior to slaughter. Blood samples were collected from sheep before loading and prior to slaughter via jugular venipuncture to assess their physiological response to transport in relation to hormonal levels. Animals were weighed just before loading onto a truck and after transport to assess shrinkage. Muscle ultimate pH, expressed juice, cooking loss percentage, WB-shear force value, sarcomere length and colour L*, a*, b* were measured on samples from longissimus dorsi, biceps femoris and semitendinosus muscles collected at 24 h postmortem at $1-3^{\circ}C$. Live weight shrinkage losses were 1.09 and 1.52 kg for 6 and 12 month transported sheep, respectively. The transported sheep had significantly (p<0.05) higher cortisol, adrenaline, noradrenaline, and dopamine concentration levels prior to slaughter at both ages than the not-transported sheep. Transportation significantly influenced meat quality characteristics of three muscles. Muscle ultimate pH and shear force values were significantly higher, while CIE L*, a*, b*, expressed juice and cooking loss were lower in transported than not-transported sheep. Age had a significant effect on meat quality characteristics of Omani sheep. These results indicated that short-term pre-slaughter transport at high ambient temperatures can cause noticeable changes in physiological and muscle metabolism responses in sheep.

• Journal of Korean Society of Transportation
• /
• v.21 no.1
• /
• pp.53-63
• /
• 2003

The Origin-Destination(OD) matrix is very important in describing transport movements in a region. The OD matrix can be estimated using traffic counts on links in the transport network and other available information. This information on the travel is often contained in a target OD matrix and traffic counts in links. To estimate an OD matrix from traffic counts, they are the major input data which obviously affects the accuracy of the OD matrix estimated, Generally, the quality of an estimated OD matrix depends much on the reliability of the input data, and the number and locations of traffic counting points in the network. Any Process regarding the traffic counts such as the amount and their location has to be carefully studied. The objective of this study is to select of the optimal location of traffic counting points for the OD matrix estimation. The model was tested in nationwide network. The network consists of 224 zones, 3,125 nodes and 6,725 links except to inner city road links. The OD matrix applied for selection of traffic counting points was estimated to 3-constrained entropy maximizing model. The results of this study follow that : the selected alternative to the best optimal counting points of six alternatives is the alternative using common links of OD matrix and vehicle-km and traffic density(13.0% of 6,725 links), however the worst alternative is alternative of all available traffic counting points(44.9% of 6,725 links) in the network. Finally, it should be concluded that the accuracy of reproduced OD matrix using traffic counts related much to the number of traffic counting points and locations.

• Journal of Korean Society of Environmental Engineers
• /
• v.35 no.3
• /
• pp.192-199
• /
• 2013

The Tandong-cheon stream is a 7.4 km long small urban stream that passes through Daeduk Science Town in Daejeon Korea. Despite the stream has great potential as an educational and recreational site due to its central location in the science town and science museums nearby, environmental aspect especially for water quality has not been evaluated properly. Through field survey, major pollution sources of the stream were identified and effect of water quality improvement alternatives were evaluated using a QUAL2K water quality model for the stream. The study indicated that controlling major pollution sources of the stream alone may not be sufficient for reaching the water quality target. Therefore, additional pollution control methods are necessary. We applied the developed model to evaluate the effects of a constructed wetland on the terrace land, and analyzed whether the water quality target can be met at the outlet of the stream. It is expected that this study would provide a good reference for environmentally sound management of small urban streams in Korea.