• Advances in Traditional Medicine
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• 제1권2호
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• pp.14-20
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• 2000

This study was designed to investigate the effect of dietary Platycodon grandiflorum on plasma glucose and lipid metabolism in $KK-A^y$ mice and STZ-induced diabetic rats. Both plasma triglyceride and plasma cholesterol levels in STZ-induced diabetic rats were significantly decreased by dietary Platycodon grandiflorum feeding for 4 weeks compared to those of control rats, but there were no marked differences in $KK-A^y$ mice. However, for plasma glucose values, Platycodon grandiflorum feeding resulted in a significant decrease in both STZ-induced diabetic rats and $KK-A^y$ mice. Also, dietary Platycodon grandiflorum slightly decreased the postprandial glucose level at 30 and 60 mins during oral glucose tolerance test in $KK-A^y$ mice. Although there was no statistical significance, the fasting plasma insulin levels of Platycodon grandiflorum dieted $KK-A^y$ mice tended to decrease when compared to that of control mice. Therefore, the present results suggested that dietary Platycodon grandiflorum may have a beneficial effect on preventing hypercholesterolemia and hyperlipidemia.

• Journal of Nutrition and Health
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• 제30권10호
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• pp.1153-1159
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• 1997

We investigated the effects of vitamin E supplementation on lipid peroxidation and on the activities of antioxidative enzymes in kidney of KK mice of various age and duration of diabetes. Weaned KK mice were fed high fat a diet containing 20% corn oil(wt/wt), and were sacrificed at 4, 6, and 9 months of age. The high vitamin E diet consisted of the high fat diet supplemented with an excessive amount of dl-$\alpha$-tocopheryl acetate (2080 IU/kg diet) . In the diabetic groups, we found an increase in lipofuscin and decrease in antioxidative enzyme activities with aging. Treatment with vitamin E did not modify the level of fasting blood glucose. However, a significant decrease in lipofuscin and increase in antioxidative enzyme acitvities were observed in diabetic mice.

• Journal of Nutrition and Health
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• 제31권2호
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• pp.153-158
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• 1998

The purpose of this study was to investigate the effect of vitamin E supplementation on the lipid peroxidation and activities of antioxidative enzymes in the pancreas of diabetic KK mice. KK mice were fed high ft diet containing 20% corn oil(wt/wt), and sacrificed at 2 months of diabetes. A hish vitamin E diet consisted of the high fat diet supplemented with an excessive amount of 이-$\alpha$-tocopheryl acetate (2080IU/kg diet). The incidence of diabetes mellitus was 61% when mice were fed the high fat diet, but was 44% when mice were fed the high vitamin E diet, Vitamin E supplementation fhus seems to have the effect of decreasing of decreasing the onset of diaetes. In the diabetic group, we found increases of MDA (malondialdehyde) and antioxidative enzyme activities. Treatment with vitamin E did not modify the level of fasting blood glucose. However, MDA and antiosicative enzyme activities in diabetic mice were decreased by the high vitamin E diet. Increased levels of lipid peroxidation products suggests the occurrence of oxidative damage in the pancreas of diabetic mice. The increased level of antiosicative enzyme activities could be due to an adaptive response to conditions of increased peroxidative stress. Significant normalization on catalase activity was noted in vitamin E supplemented animals.

• The Korean Journal of Physiology
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• 제17권2호
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• pp.183-190
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• 1983

Effects of water extracts of antler on blood sugar levels were studied in normal rats and KK-mice. a spontaneously diabetic strain of murine. Blood sugar levels were determined at 30, 60, and 120 minutes after intraperitoneal administration of 1 ml/kg or 2ml/kg AE into normal rats. A moderate but significant decrease in blood sugar level was noticed only in 30 minutes following 2 ml/kg AE administration. On the other hand no significant changes in blood sugar level was observed in KK-mice to which 1ml/kg/day AE had been given for 10 weeks. The results of present study strongly indicate that antler extract exerts no significant effect on blood sugar levels in experimental animals.

• 한국식품영양과학회지
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• 제26권5호
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• pp.914-919
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• 1997

We investigated the effects of vitamin E supplementation on the protein glycosylation in vivo. Weaned KK-mice were fed high fat diet containing 20% corn oil(wt/wt), and sacrificed at 4, 6, and 0 months of age. High vitamin E diet was the high fat diet supplemented with an excess amount of 이-$\alpha$-tocopheryl acetate(2080IU/kg diet). We measured $HbA_{1C}$ as a glycosylation early product, and collagen-linked fluorescence (CLF) of skin as a glycosylation and product. We found that diabetic group had increased levels of $HbA_{1C}$ within 2 months after onset of diabetes and during the experiments. The skin CLF increased dramatically 5 months after onset of diabetics. Treatment with vitamin E did not modify the level of blood glucose. However, we observed a significant lowering in CLF and $HbA_{1C}$ in diabetic mice.

• 한국식품영양과학회지
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• 제27권1호
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• pp.149-156
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• 1998

The purpose of this study was to investigate the effects of vitamin E supplementation on the activities of antioxidative enzymes in liver of KK mice of various ages and various duration of diabetes. Diabetes was induced by feeding high fat diet containing 20% corn oil(wt/wt). Weaned KK mice were fed high fat diet containing 51 IU or 2080 IU vitamin E per kg diet. Animals were sacrificed at 4, 6, and 9 months of age. In nondiabetic group, we found the decrease of antionxidative enzyme activities with aging. In diabetic group, antioxidative enzyme activities were decreased, and the change of hepatic vitamin E was related to glutathione peroxidase activity (r=0.71, p<0.001). Treatment with vitamin E did not modify the level of fasting blood glucose. However, it was observered that glutathione reductase and glutathione peroxidase activities as well as hepatic glutathione levels were increased by vitamie E supplementation, whereas catalase activity did not changed. The present result suggest that high vitamin E supplementation protects against lipid peroxidative damage in diabetic KK mice.

• 한국식품영양과학회지
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• 제46권2호
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• pp.267-272
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• 2017

본 연구에서는 팔미틴산을 처리한 지방간질환 간세포 모델과 비만/당뇨 동물 모델인 KK/HlJ 마우스를 이용하여 피세아테놀과 레스베라트롤 투여가 염증조절에 주는 영향을 알아보고자 하였다. 4주간의 피세아테놀과 레스베라트롤 섭취는 공복혈당과 경구당부하 검사 2시간 후 AUC를 감소시켜 혈당 조절 개선 효과를 보였다. 또한, 팔미틴산을 처리한 지방간질환 간세포 모델에 피세아테놀과 레스베라트롤을 처리한 결과 염증조절 경로인자인 TLR4와 $NF-{\kappa}B$의 발현을 유의적으로 감소시켰다. 이를 in vivo 비만/당뇨 동물 모델인 KK/HlJ 마우스의 간 조직에서 확인한 결과 피세아테놀 섭취는 NLRP3와 $NF-{\kappa}B$의 간 조직에서의 발현을 유의적으로 감소시켰고, IL-1 발현을 감소시키는 경향을 보였다. 하지만 동량의 레스베라트롤 섭취는 이와 같은 항염증 효과를 보이지 않았다. 결론적으로 혈당 조절 개선 효과와 항염증 효과에 있어 피세아테놀이 레스베라트롤보다 우수한 효과를 가지고, 피세아테놀의 항염증 효과는 혈당 조절 개선 효과에 부분적으로 기여할 것으로 제안한다.

• Journal of Nutrition and Health
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• 제31권6호
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• pp.1024-1030
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• 1998

KK마우스에 고지방 식이를 섭취시켜 당뇨를 유발하고, Maillard 반응의 초기산물인 Hb $A_{IC}$와 후기 산물인 신장조직의 CLF가 증가되는지를 확인하고. 당뇨병에 의해 증가되는 Maillard 반응이 비타민 I의 투여로 억제되는지 관찰하였다. 또 신장조직의 광학 현미경 관찰을 통하여 신장병변을 검사하였다. 이유즉시 KK마우스를 1개월간 pellet diet로 적응시킨 후 저비타민 E 식이(corn oil 20%, wt/wt)를 먹여 당뇨를 유도하였다. 비타민 E 보강은 2080IU/kg diet 수준으로 하였으며. 생후 4개월 , 6개월 . 9개월(당뇨 0개월. 2개월, 5개월)에 희생시켰다. 정상 KK-마우스에서는 월령이 증가하였을 때. 혈당과 Hb $A_{IC}$는 유의적인 변화를 보이지 않았으나 신장조직의 CLF는 점진적으로 증가되었다. 당뇨군에서 Hb $A_{IC}$는 6개월에서 증가되기 시작하였고 9개월군과 차이가 없었으며 . 신장조직의 CLF는 9개월 군에서 급격히 증가하였다. 또 고비타민 E 식이를 섭취시킨 경우 당뇨군에서 초기 및 후기 Maillard 반응생성물의 감소가 관찰되었다. 이상의 결과로부터 KK마우스에서는 월령이 증가함에 따라 초기 및 후기 당화산물의 축적과 신장 병변의 변화가 관찰되었고, 또한 당뇨 유발에 의해 이 변화들은 더욱 촉진되었으나, 2080IU/kg diet 수준의 고비타민 E 식이를 섭취시켰을 때 이런 변들이 억제되었음을 확인하였다.확인하였다.

• Journal of Nutrition and Health
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• 제37권5호
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• pp.347-351
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• 2004

This study was carried out to investigate the supplementary effects of ${\gamma}$-oryzanol extracted from rice bran on lipid metabolism in diabetic mice. We supplied 2 kinds of experimental diets (CO without and GO with 0.2％ ${\gamma}$-oryzanol) to diabetic mice for 8 weeks. Diet intake, body weight, organ weight, contents of serum and hepatic lipid profiles, and fecal lipid levels were measured. Though there was no significant difference in diet intake, body weight change and organ weight between experimental groups, the concentration of serum total cholesterol and hepatic total lipid, total cholesterol and HMG-CoA reductase activity was significantly lower in GO group treated with 0.2％ ${\gamma}$-oryzanol of diet than CO group after supplementary period of experimental diets. And total lipid, triglyceride, total cholesterol, and bile acid levels excreted to feces were significantly higher in GO group than CO group. These results suggest that ${\gamma}$-oryzanol decrease the serum and hepatic lipid levels by lowing HMG-CoA reductase activity or increasing the contents of fecal lipid in diabetic KK mice.

• 한국동물학회지
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• 제35권3호
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• pp.372-382
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• 1992

당뇨병유발제인 streptozotocin이 생쥐 신장 사구체곁세포의 미세구조에 어떠한 영향을 미치는 지를 알아보고자 일반계통인 ICR생쥐와 유전성 당뇨병계통인 KK생쥐에 streptozotocin을 투여하여 경시적으로 각 동물의 신장 사구체곁세포의 미세구조의 변화를 관찰하였다. Streptozotocin을 투여한 ICR생쥐의 사구체곁세포는 3일째부터 과립형질내세망의 미약한 팽창과 과립내에 대소 공포의 출현 및 용해소체가 간혹 관찰되었다. 그후 시간이 지남에 따라 더욱 심하여 특히 2주 및 4주에서는 과립형질내세망의 팽창, 사립체, 골지장치 및 리보소곤 등이 소수 출현하였는데 비해 대소 용해소체는 많이 관찰되었으며 심한 탈과립으로 인해 세포질내 과립의 면적이 현저히 감소되었다. 그러나 KK생쥐의 실험군에서는 전 실험군에 걸쳐 퇴행성변화가 적었으며 ICR 생쥐 실험군에 비해 그 영향이 훨씬 적었다. 이상의 결과를 종합하여 보면 정상 ICR생쥐에 streptozotocin을 투여하자 되면 ICR생쥐 사구체곁세포에서 과립의 유의한 감소 및 세포내 미세구조의 퇴행성변화가 뚜렷한데 비해 KK생쥐 실험군에서는 ICR생쥐 실험군에 비해 손상을 적게 받았는데 이는 KK생쥐가 갖고 있는 당뇨병에 대한 내성에 의해 영향을 적게 미치는 것이 아닌가 추측된다.