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Radioimmunoassay Using Quality Control Materials BIO-RAD of the Suggestions for Setting Acceptable Range (BIO-RAD 정도관리 물질을 이용한 방사면역측정법의 Acceptable Range 설정에 대한 제언)

  • Park, Hee-Won;Kim, Tae-Hoon;Roh, Gyeong-Un;Kim, Hyun-Joo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.1
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    • pp.108-114
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    • 2012
  • Purpose: Immunosorbent assay most commonly used in the laboratory and commercial third-party quality control material is a substance that provided by BIO-RAD. However, in Reference Sheet by radioimmunoassay test kit or a measuring device for the mention of Acceptable Range is somewhat lacking. Radioimmunoassay for the inspection of test results by setting Acceptable Range to increase the objectivity of the recommendations on the data accumulated by the manufacturer listed in the Reference Sheet is to be issued. Materials and Methods : In our hospital since 2009 partially BIO-RAD using quality control materials in 2011, excluding certain items, some items were most of the BIO-RAD third-party quality control materials are used. Thus, internal quality control data accumulated BIO-RAD's Unity Real Time program by using the items were measured. Results : BIO-RAD using quality control material items were about 50 of the 20 Point Data averages, standard deviations, variation coefficients were calculated to measure the Acceptable Range of kit, automated immunoassay attributed Roche Elecsys / E170 / cobas e Systems the measures and compared. Conclusions : BIO-RAD QC materials commonly used hospital and peer group by setting the measurement kit, suitable for laboratory equipment for radioimmunoassay of Acceptable Range manufacturer recommendation to increase the objectivity of the test results by national and international recognition for radioimmunoassay should seek to increase.

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Korean Paddy Soil Microbial Community Analysis Method Using Denaturing Gradient Gel Electrophoresis (Denaturing gradient gel electrophoresis를 이용한 한국의 논 토양 미생물 다양성 분석 방법)

  • Choe, Myeongeun;Hong, Sung-Jun;Lim, Jong-Hui;Kwak, Yunyoung;Back, Chang-Gi;Jung, Hee-Young;Lee, In-Jung;Shin, Jae-Ho
    • Journal of Applied Biological Chemistry
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    • v.56 no.2
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    • pp.95-100
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    • 2013
  • Soil microbes are important integral components of soil ecosystem which have significant and diverse role in organic matter decomposition, nitrogen cycling, and nitrogen fixation. In this study an effective denaturing gradient gel electrophoresis (DGGE) method was employed for paddy soil microbial diversity survey. For optimum paddy soil microbial DNA extraction, different methods such as Lysis buffer, skim milk bead, sodium phosphate buffer, Epicentre Soil Master DNA extraction kit (Epicentre, USA) and Mo Bio Power Soil DNA kit (MO BIO, USA) methods were utilized. Among all the method, using Mo Bio Power Soil kit was most effective. DGGE analysis of Bacteria was carried out at 6% polyacylamide gel and 45-60% denaturing gradient in the optimal conditions. Whereas DGGE analysis of fungi was done at 6% polyacrylamide gel and 45-80% denaturing gradient in the optimal conditions. By applying the above assay, it was found that variation within the microbial community of paddy soil occurs by a factor of time. DGGE assay used in this study through for a variety of soil microbial analysis suggests the potential use of this method.

Reliability Design of the Hinge Kit System in Common Refrigerator Under Repetitive Load (상용 냉장고에서 반복 하중을 받는 힌지 키트 시스템(HKS)의 신뢰성 설계 연구)

  • Woo, Seong-woo;Lee, Jongkil
    • 대한공업교육학회지
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    • v.33 no.2
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    • pp.312-324
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    • 2008
  • To improve the newly designed HKS(hinge kit system) in common refrigerators, it was investigated the new robust methodologies. There were the study of failure modes, mechanisms in the marketplace, and the design parameters of HKS with various improvements using accelerated life testing. Based on the claimed marketplace product returns and 1st ALT reproduction, the fracturing and cracking occur in the housing of the HKS. The missing design parameters of the failed HKS in the design phase of the refrigerator was the housing hinge kit structure. The corrective action plans are the modifications of the housing hinge kit structure from the open supporting to all supporting structure. Based on 2nd ALTs, the fracturing and cracking occur in the torsion shaft. The missing design parameter was the roundness of torsion shaft. After a sequence of ALT testing, the levels of the missing design parameters were setup. The yearly failure rate and B1 life of the redesigned HKS, based on the results of ALT, were over 0.01 percent and 10 years, respectively. The parameter design through the inspection of the failed product, load analysis, and three rounds of ALT, was very effective in the new robust design methodologies of the mechanical system and this method can be applied to other design system.

A Study on the Estimation Accuracy of Energy Expenditure by Different Attaching Position of Accelerometer (가속도계의 부착위치에 따른 에너지 소비량의 예측 정확도에 관한 연구)

  • Kang, Dong-Won;Choi, Jin-Seung;Mun, Kyung-Ryoul;Bang, Yun-Hwa;Tack, Gye-Rae
    • Korean Journal of Applied Biomechanics
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    • v.19 no.1
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    • pp.179-186
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    • 2009
  • This works studied to compare gas analyzer with accelerometer and the estimation of energy expenditure based on different attaching position of tri-axial accelerometer such as waist and top of the foot Based on the fact that oxygen intake increases more radically linearly during walking more than 8.0km/hr. 9 male subjects performed walking and running on the treadmill with speed of $1.5{\sim}8.5km$/hr and $4.5{\sim}13.0km$/hr, respectively. Commercially available Nike + iPod Sports kit was used to compare energy expenditure with sensor module attached to their foot. Actual energy expenditure was determined by a continuous direct gas analyzer and two multiple regression equations of walking and running mode for different attaching position were developed. Results showed that estimation accuracy of energy expenditure using waist mounted accelerometer was higher than that of the top of the foot and Nike + iPod Sports kit. Results of energy expenditure based on waist and top of the foot showed that the crossover state of energy expenditure occurred at 7.5km/hr. But Nike + iPod Sports kit could not find intersection of energy expenditure in all nine subjects. Therefore the sensor module attached to the waist and separate multi regression equation by walking and running mode was the best to estimate more accurate prediction.

Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis (소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가)

  • Hong, Leegon;Choi, Woojae;Ro, Younghye;Ahn, Sunmin;Kim, Eunkyung;Choe, Eunhee;Kim, Danil
    • Korean Journal of Veterinary Service
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    • v.43 no.4
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    • pp.201-209
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    • 2020
  • In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon-gamma (IFN-γ) is included in Korea's diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM®) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM® and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM®, respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM® kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TB-Feron Plus kit was not inferior to BOVIGAM® in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.

A Study on Photonic sensor Interface in SOPC platform (SOPC기반 광-센서 인터페이스에 관한 연구)

  • Son, Hong-Bum;Park, Seong-Mo
    • Proceedings of the IEEK Conference
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    • 2005.11a
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    • pp.971-974
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    • 2005
  • In this paper, we describe photonic sensor interface in SOPC(System on a programmable chip) platform. This platform uses device that has ARM922T processor and APEX FPGA area on a chip. We use two development kits. The one is embedded kit that using Intel's Xscale device, the another is SOPC kit that using Altera's Excalibur device. We implement some device logic that DMAC, ADCC, etc. and application.

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SOME RESULTS ON THE QUESTIONS OF KIT-WING YU

  • Majumder, Sujoy
    • Communications of the Korean Mathematical Society
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    • v.31 no.2
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    • pp.295-309
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    • 2016
  • The paper deals with the problem of meromorphic functions sharing a small function with its differential polynomials and improves the results of Liu and Gu [9], Lahiri and Sarkar [8], Zhang [13] and Zhang and Yang [14] and also answer some open questions posed by Kit-Wing Yu [16]. In this paper we provide some examples to show that the conditions in our results are the best possible.

Electrical, optical, and thermal properties of AZO co-sputtered ITO electrode for organic light emitting diodes

  • Park, Young-Seok;Kim, Han-Ki
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.416-419
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    • 2008
  • In this study, we report on the characteristics of Aldoped ZnO (AZO) co-sputtered indium tin oxide (ITO) films prepared by dual target direct current (DC) magnetron sputtering at room temperature for organic light emitting diodes (OLEDs). The electrical and optical properties of co-sputtered IAZTO electrode were critically dependent on the DC power of AZO. Furthermore, the characteristics of co-sputtered IAZTO electrode were influenced by rapid thermal annealing temperature.

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Bonding Mechine for Axiomatic Design (공리적 설계 방법에 의한 Bonding Mechine의 개념 설계)

  • Kim, Won-Jong;Hwang, Eun-Ha
    • Journal of the Korean Society of Industry Convergence
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    • v.17 no.4
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    • pp.194-202
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    • 2014
  • Bonding Machine is new concept of semi industry. In this study, bonding machine is designed with aximatic design, then manufactured based on it. Axiomatic design offers a scientific base for design in an efficient way. Many application of the independence axiom have been pulished, however, the information axiom has been mainly applied to FR - DP problems except for few case studies.