• Journal of Marine Life Science
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• v.1 no.1
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• pp.41-49
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• 2016

Kisspeptin (Kiss) and its cognate receptor, kisspeptin receptor (KissR; G protein coupled receptor 54, GPR54), have recently been recognized as potent regulators of reproduction in teleosts. Additionally, leptin plays an important role in energy homeostasis and reproductive function in teleosts. The purpose of this study was to examine differences in the concentration of the hormones of the Kiss/KissR system and leptin and the expression of their underlying genes, all of which are involved in the sexual maturation of female goldfish, Carassius auratus, following treatment with Kiss. The expression levels of KissR increased after the Kiss injection. Furthermore, the peptide hormone leptin also increased after the injection (in vivo and in vitro). Additionally, the expression of GnRH and GTHs (GTHα, FSHβ, and LHβ) increased in the brain and pituitary (in vitro and in vitro). These results support the hypothesis that Kiss plays important roles in the direct regulation of the hypothalamus-pituitary-gonad axis and leptin in goldfish. Therefore, we suggest that Kiss system gene expression is correlated with energy balance and reproduction.

• Journal of Technology Innovation
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• v.12 no.2
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• pp.209-225
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• 2004

This study aims to explore and understand the role of knowledge intensive service activities in the industrial innovation of Korea. It analyzes public and private service inputs to the innovation of manufacturers. The contribution of KISs to the innovation of both service suppliers and manufacturers was analyzed by using survey questionnaire. The results of the analysis revealed that the contribution of KISs to the innovation capability of manufacturers is significant. Large manufacturers that hold internal capability to supply KISs are also active in using external KISs. The manufacturing firms that utilized KISs intensively proved to be more innovative than those did not. Heavy KISs users also entertained benefits of capability enhancement as they improved monitoring and achieved efficient application of knowledge asset into product and process innovation. The role of public KISs in the innovation of manufacturers appeared to be indirect and done mainly through education and public R&D activities. Direct input, such as public software service, was not utilized as much in service suppliers themselves. The major reason is that public KISs may not be relevant to their actual needs. Manufacturers have implemented both tighter integration of familiar KISs and loosely coupled unfamiliar KISs.

• International Journal of Highway Engineering
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• v.15 no.5
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• pp.145-156
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• 2013

PURPOSES : This research is a study on the changes in the road network of the surrounding area is installed according to the Kiss & Ride Zone. METHODS : Estimating the transportation mode of students by using the Metropolitan household Surveys(2006) and estimating the O/D by Kiss & Ride ratio with the estimated data, then being applied to a method of reducing the number of lanes for certain sections of the road which would be installed with Kiss & Ride Zone. RESULTS : The reason why it is different for delay resolving time and the affected roads as the Kiss & Ride percentage change, was the impact of the Kiss & Ride Zone's installation position. CONCLUSIONS : The purpose of the study was to analyze the impact of the road network in accordance with the installation of Kiss & Ride Zone by using speed and queue delay resolving time, and it is a need to develop a quantitative evaluation technique which was using various indicators in impact analysis according to the installation of the traffic safety facilities in the future.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.3
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• pp.372-378
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• 2016

Clutch torque control is the key to the ride comfort improvement of a vehicle equipped with AMT (automated manual transmission). For such control, the torque transfer starting point, known as the "kiss point," should be indicated or at least estimated to compensate for the clutch torque. The kiss point changes due to wear, high temperature, and fatigue; as such, it should be estimated while the vehicle is being driven. In this study, the method of kiss point active estimation for an AMT vehicle with a dry-type clutch was devised. The kiss point is learned while the engine is in an idle state and while the transmission is at a neutral gear position. It is determined when the input shaft of the transmission starts to rotate by slowly engaging the clutch. The noise of the shaft speed signal during the slow engagement process is filtered for accurate control. The kiss point estimation at various clutch engagement speeds was analyzed via a vehicle test.

• Development and Reproduction
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• v.16 no.1
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• pp.31-38
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• 2012

Kisspeptin has been implicated in the process of puberty onset in various animal groups. This peptide is encoded by a gene, Kiss1 in avian and mammalian species. Contrary to these higher vertebrates, however, fish appeared to have another gene, Kiss2 that also codes for the precursor peptide of kisspeptin. To figure out biological significance of this gene during the puberty onset in fish, the expression profile of Kiss2 gene was investigated in the brain of Nile tilapia together with genes of GPR54, GnRH receptorI (rGnRHI) and GTH subunits ($LH{\beta}$ and $FSH{\beta}$). Expression of Kiss2 mRNA significantly increased at 2 weeks post hatch (wph) and 13 wph ($P$<0.05). This increase coincided with the increases of GPR54 and rGnRH I gene expression. Detection of $LH{\beta}$ and $FSH{\beta}$ subunit gene expression was possible later than 13 wph, indicating the activation of gonadotrophs in the pituitary. Data obtained from this study strongly suggest that, in addition to Kiss1 gene, Kiss2 gene is deeply associated with the onset of puberty by the activation of hypothalamus pituitary gonadal axis in Nile tilapia.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2002.09a
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• pp.19-27
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• 2002

The four agencies in Korea - KMA, KIGAM, KEPRI, and KINS - have been operating their own seismic network for many years. In this study we have developed an integrated seismic system named KISS (Korea Integrated Seismic System), which is very similar to LISS (Live Internet Seismic Server) of Albuquerque Seismological Laboratory. Through KISS we could share all the earthquake data observed by those organizations in near real time. This research result will lead to provide the opportunity to use all seismic information of the earthquakes around Korean peninsula. And KISS will make us enable to do systematic researches, such as study on focal mechanisms of earthquakes around Korean peninsula, seismic design, earthquake prediction, etc. KISS will be used in developing an Early Earthquake Warning System like TriNet in Southern California, USA so as to minimize seismic hazard.

• JSTS:Journal of Semiconductor Technology and Science
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• v.13 no.4
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• pp.282-290
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• 2013

This paper describes a spread spectrum clock generator (SSCG) circuit for DisplayPort 1.2 standard. A Hershey-Kiss modulation profile is generated by dual sigma-delta modulators. The structure generates various modulation slopes to shape a non-linear modulation profile. The proposed SSCG for DisplayPort 1.2 generates clock signals with 5000 ppm down spreading with a Hershey-Kiss modulation profile at three different clock frequencies, 540 MHz, 270 MHz and 162 MHz. The measured peak power reduction is about 15.6 dB at 540 MHz with the chip fabricated using a $0.13{\mu}m$ CMOS technology.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.211-220
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• 2015

We investigated the differences in the expression of the neurohormones kisspeptin (Kiss) and gonadotropin-inhibitory hormone (GnIH) and cytochrome P450 aromatase (P450arom), gonadotropin hormones (GTHs), and sex steroids in the goldfish Carassius auratus exposed to light-emitting diodes (LEDs). The expression levels of Kiss1, Kiss2, G-protein-coupled receptor 54 (GPR54), GTHs, GnIH, and P450arom were compared between the control (white light) and LED-treated goldfish. Furthermore, we measured the plasma levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The levels of Kiss1 mRNA and protein; Kiss2, GPR54, and $GTH{\alpha}$ protein; GTH mRNA; and plasma FSH and LH in the hypothalamus and cultured hypothalamus cells were significantly higher in the green and purple LED treatment groups than in the other groups. These results suggested that red LEDs inhibit the sex maturation hormones, Kiss, GPR54, GTHs, and P450arom, and that GnIH plays a role in the negative regulation of reproductive function in goldfish.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.6
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• pp.835-841
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• 2018

Objective: The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods: Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. Results: The specific anti-KISS1 antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS (KISS1-hepatitis B surface antigen S) fusion fragment of KISS1 gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. Conclusion: The effects of KISS1 gene vaccine in immunocastration were reversible and no integration events were recorded.

• Ocean and Polar Research
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• v.38 no.1
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• pp.81-88
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• 2016

In many fish species, including Nile tilapia (Oreochromis niloticus), gonadal development occurs at the expense of stored energy and nutrients. Therefore, reproductive systems are inhibited by limited food supply. It has been well established that reproductive function is highly sensitive to both metabolic status and energy balance. Nothing is known about the possible mediated connection between energy balance and reproduction. Kisspeptin, a neuropeptide product of the Kiss gene has emerged as an essential gatekeeper of reproduction and may be possibly be linked to energy balance and reproduction in non-mammalians. Thus, in this study, the effect of fasting (10 days) on the expression of kisspeptin and the gonadotropin-releasing hormone (GnRH) gene were assessed in Nile tilapia (male and female) using qRT-PCR. In addition, plasma levels of estradiol-$17{\beta}$ ($E_2$) and 11-ketotestosterone (11-KT) in adult tilapia were measured by ELISA. In male tilapia, fasting reduced Kiss2 and GnRH I mRNA expression in the brain and 11-KT level in comparison with the fed tilapia (p < 0.05). In females, however, there were no significant differences in GnRH I mRNA expression and $E_2$ between fish subjected to fasting and those fed (p > 0.05). These data indicate the impact of nutritional states on kisspeptin as a potential regulatory mechanism for the control of reproduction in male Nile tilapia.