• 제목/요약/키워드: KFDA

검색결과 710건 처리시간 0.038초

SAFETY EVALUATION OF ADENOVIRUS-MEDIATED P16 GENE TRANSFER BY USING MICROARRAY AND 2D/MALDI-TOF

  • Park, Misun;Hoil Kang;Jaehee Pyo;Sinae Lim;Seungwan Jee;Miok Eom;Taikyung Ryeom;Kim, Okhee
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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    • pp.196-196
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    • 2002
  • p16INK4a tumor suppressor gene transfer in the non-small cell lung cancer cells by transduction of recombinant adenovirus (Ad5CMV-p16) resulted in significant inhibition of cancer cell growth (Anticancer Res., 1998, 18:3257-3261). As a safety concern, we have investigated gene and protein expression after transduction of adenoviral vector (Ad5CMV-p16) in human non-small cell lung cancer (A549) cells by using microarray and 2D gel electrophoresis/ MALDI-TOF.(omitted)

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Differential Protein and Gene Expression after Adenovirus-Mediated p16 Gene Transfer in Human Non-Small Cell Lung Cancer Cells

  • Park, Mi-Sun;Kang , Ho-Il;Jee, Seung-Wan;Lim, Si-Nae;Pyo, Jae-Hee;Eom , Mi-Ok;Ryeom , Tai-Kyung;Kim, Ok-Hee
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.291.2-291.2
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    • 2002
  • For the safety evaluation of adenovirus-mediated gene therapy. we have investigated gene and protein expression after transduction of adenoviral vector (Ad5CMV-p16) which contains tumor suppressor gene. p161NK4$\alpha$ in human non-small cell lung cancer (A549) cells. We compared the differential gene expression level in the A549 cells treated with Ad5CMV (null type) and Ad5CMV-p16 virus. respectively. by using cDNA membrane chip and oligonucleotide chip. (omitted)

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Effect of organophosphorous pesticides as neuro-endocrine disruptors on the immune system of Balb/c mice

  • Kim, Hyung-Soo;Juno H. Eom;Chung, Seung-Tae;Park, Jae-Hyun;Kil, Jung-Hyun;Lee, Jong-Kwon;Oh, Hye-Young
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2003년도 춘계학술대회 논문집
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    • pp.78-79
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    • 2003
  • Organophosphorus (OP) pesticides have largely replaced the use of organochlorine pesticides and have been widely used in agriculture and houses in recent years because of their rapid breakdown in water and their low environmental persistence. As a result, consumers are directly or indirectly exposed to organophosphorus pesticides through several food groups including meat, dairy products, fruits, vegetables, dried foods, and most processed food in which a significant amount of pesticide residues have been found.(omitted)

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Neoplastic Transformation of Immortalized Human Keratinocytes by 2,3,7,8-Tetrachlorodibenzo-P-Dioxin

  • Kang, Mi-Kyung;Kang, Ho-Il;Park, Young-Sill;Ryeom, Tai-Kyung;Eom, Mi-Ok;Park, Mi-Sun;Jee, Seung-Wan;Kim, Ok-Hee
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2003년도 추계학술대회
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    • pp.178-178
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    • 2003
  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a prototype of many halogenated aromatic hydrocarbons, is a ubiquitous, persistent environmental contaminant and the most powerful carcinogen categorized by IARC. It is display high toxicity in animals and is associated with several cancers in human. Although the mechanism of carcinogenesis by TCDD is unclear, it is considered to be a non-genotoxic and rumor promoter.(omitted)

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Immunotoxicity of Organophosphorous Pesticides, Pirimiphos-Methyl and Methidathion on Mouse Spleen Cells in Vitro

  • Juno H. Eom;Park, Jae-Hyun;Byun, Jung-A;Cho, Hye-Young;Lee, Jong-Kwon;Oh, Hye-Young;Kim, Hyung-Soo
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2003년도 추계학술대회
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    • pp.183-183
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    • 2003
  • In recent years, organophosphorous (OP) pesticides have largely replaced the use of organochlorine pesticides and have been widely used in agriculture because of their rapid breakdown in water and their low environmental persistence. Consumers are directly or indirectly exposed to organophosphorous pesticides through several food groups in which a significant amount of pesticide residues have been found.(omitted)

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Epigallocatechin-3-gallate, a green tea polyphenol, reduces MPTP-induced neurotoxicity by down-regulation of inducible nitric oxide synthase

  • Suh, Soo-Kyung;Kim, Jong-Min;O, Jeong-Ja;Park, Chang-Won;Seo, Kyung-Won
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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    • pp.198-198
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    • 2002
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