• BMB Reports
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• v.40 no.1
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• pp.44-52
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• 2007

Kinesins, as a kind of microtubule-based motor proteins, have a conserved microtubule-binding site in their motor domain. Here we report that two homologous kinesins in Arabidopsis thaliana, KatB and KatC, contain a second microtubule-binding site in their tail domains. The prokaryotic-expressed N-terminal tail domain of the KatC heavy chain can bind to microtubules in an ATP-insensitive manner. To identify the precise region responsible for the binding, a serious of truncated KatC cDNAs encoding KatC N-terminal regions in different lengths, KatC1-128, KatC1-86, KatC1-73 and KatC1-63, fused to Histidine-tags, were expressed in E. coli and affinity-purified. Microtubule cosedimentation assays show that the site at amino acid residues 74-86 in KatC is important for microtubule-binding. By similarity, we obtained three different lengths of KatB N-terminal regions, KatB1-384, KatB1-77, and KatB1-63, and analyzed their microtubule-binding ability. Cosedimentation assays indicate that the KatB tail domain can also bind to microtubules at the same site as and in a similar manner to KatC. Fluorescence microscopic observations show that the microtubule-binding site at the tail domain of KatB or KatC can induce microtubules bundling only when the stalk domain is present. Through pull-down assays, we show that KatB1-385 and KatC1-394 are able to interact specifically with themselves and with each other in vitro. These findings are significant for identifying a previously uncharacterized microtubule-binding site in the two kinesin proteins, KatB and KatC, and the functional relations between them.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.313-323
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• 2014

Significant alteration in the microbial community can occur across reclamation areas suffering subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, and Firmicutes. However, the bacterial communities' abundance, diversity, and composition differed significantly between the sites. Site A presented higher bacterial diversity and more complex community structures than site B. The majority of sequences related to Proteobacteria, Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria, and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria, and Phycisphaerae originated from site B. The distribution of some bacterial groups and subgroups in the two sites correlated with soil properties and vegetation due to reclamation practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were important factors shaping the underlying microbial communities. Furthermore, the specific plant functional group (legumes) was also an important factor influencing soil microbial community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil bacterial diversity was validated and an association between land ecological system restoration, mostly mediated by microbial communities, and an improvement in soil properties in coal-mining reclamation areas was suggested.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2023.05a
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• pp.367-368
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• 2023

Kiscon, Seumteo, KONEPS and CSI are representative construction-related DBs. All four DBs are operated by the public. However, the characteristics of data are different depending on the purpose. Therefore, it is difficult to utilize integrated data and it is only used sparingly. Creating and sharing a unique key that can identify a construction site will enable integrated accumulation and management of construction-related data for various purposes. At this point, it is most efficient to assign a unique key based on KISCON. KISCOn data conforms to the construction site definition and covers most of the public, private, architectural and civil works. In addition, there is an advantage in that DB construction is performed in the construction situation, which is a relatively preceding process. In the future, it is necessary to create a practical construction site integrated DB through the production of an integrated key table containing linkage information of unique keys for site management, performance indicators, and statistics production.

• BMB Reports
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• v.45 no.8
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• pp.452-457
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• 2012

Diketoreductase (DKR) from Acinetobacter baylyi contains two tryptophan residues at positions 149 and 222. Trp-149 and Trp-222 are located along the entry path of substrate into active site and at the dimer interface of DKR, respectively. Single and double substitutions of these positions were generated to probe the roles of tryptophan residues. After replacing Trp with Ala and Phe, biochemical and biophysical characteristics of the mutants were thoroughly investigated. Enzyme activity and substrate binding affinity of W149A and W149F were remarkably decreased, suggesting that Trp-149 regulates the position of substrate at the binding site. Meanwhile, enzyme activity of W222F was increased by 1.7-fold while W222A was completely inactive. In addition to lower thermostability of Trp-222 mutants, molecular modeling of the mutants revealed that Trp-222 is vital to protein folding and dimerization of the enzyme.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.534-538
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• 2001

The xynA gene encoding an alikali-tolerant endo-1,4-${\beta}$-xylanase (XYN) was cloned from the alkalophilic Bacillus pumilus A-30. The nucleotide sequence of a 974-bp DNA fragment containing the xynA was determined. An ORF of 684 nucleotides that encoded a protein of 228 amino aicds was detected. Asparagine-71 of XYN from B. Pumilus A-30 showed to be highly conservative in alkaline xylanases of family G/11, upon comparing the amino acid sequences of 17 family G/11 xylanases. Site-directed mutation of N71D of the xynA gene resulted in a decrease of 12.4% in the specific acitivity and a significant decline in the enzyme activity in the alkaline pH range.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.06a
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• pp.52-57
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• 2004

In order to analyze key parameters of important radionuclides in dose evaluation model of decommissioning site, a sensitivity analysis was performed. This analysis assumed a resident farmer for an exposure scenario and 0.037Bq/g for the concentration of radionuclides. As a result of sensitivity analysis, the key parameters of radionuclides considered were the area of contaminated zone, external gamma shielding factor and indoor time fraction for Cs-137 and Co-60. The key parameters for C-14 were the environmental parameters and hydrological parameters of unsaturated zone. Also, the key parameter for Sr-90 was the density of contaminated zone.

• Bulletin of the Korean Chemical Society
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• v.32 no.6
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• pp.1851-1858
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• 2011

Details of the double-bond isomerization of 1-hexene over H-ZSM-5 were clarified using density functional theory. It is found that the reaction proceeds by a mechanism which involves the Br${\o}$nsted acid part of the zeolite solely. According to this mechanism, 1-hexene is first physically adsorbed on the acidic site, and then, the acidic proton transfers to one carbon atom of the double bond, while the other carbon atom of the double bond bonds with the Br${\o}$nsted host oxygen, yielding a stable alkoxy intermediate. Thereafter, the Br${\o}$nsted host oxygen abstracts a hydrogen atom from the $C_6H_{13}$ fragment and the C-O bond is broken, restoring the acidic site and yielding trans-2-hexene. The calculated activation barrier is 12.65 kcal/mol, which is in good agreement with the experimental value. These results well explain the energetic aspects during the course of double-bond isomerization and extend the understanding of the nature of the zeolite active sites.

• Earthquakes and Structures
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• v.9 no.1
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• pp.173-194
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• 2015

Site response analysis is an important topic in earthquake engineering. A time-domain numerical method called as one-dimensional (1D) finite element artificial boundary method is proposed to simulate the homogeneous plane elastic wave propagation in a layered half space subjected to the obliquely incident plane body wave. In this method, an exact artificial boundary condition combining the absorbing boundary condition with the inputting boundary condition is developed to model the wave absorption and input effects of the truncated half space under layer system. The spatially two-dimensional (2D) problem consisting of the layer system with the artificial boundary condition is transformed equivalently into a 1D one along the vertical direction according to Snell's law. The resulting 1D problem is solved by the finite element method with a new explicit time integration algorithm. The 1D finite element artificial boundary method is verified by analyzing two engineering sites in time domain and by comparing with the frequency-domain transfer matrix method with fast Fourier transform.

• Architectural research
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• v.25 no.1
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• pp.11-17
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• 2023

This study aims to derive key construction supervision work that can be digitalized among construction supervision work. The scope of the study includes domestic construction sites to which the Building Act of South Korea is applied. First, the construction supervision process was identified through extensive consultation of construction expert witnesses. Afterwards, the key construction supervision works for digitization were derived through survey results construction expert witnesses. Among the 24 supervision works throughout the pre-construction, construction, and post-construction stages, the proportion of supervision work manpower or cost, the type of data generated by supervision work and the urgency of introducing digital technology, and the impact on productivity and safety of supervision work were evaluated on a 5-point scale from "very low" to "very high" was investigated. The survey was conducted by t-test analysis, and as a result, it was possible to derive that the level of digitization was higher than average in "quality management," "safety management," and "collection and cooperation of maintenance instructions." It can be interpreted that digitization has been introduced on a pilot testing basis or has already been completed according to the needs of the site. On the other hand, it can be interpreted that the work that are obtained below the average score are considered to be realistically constrained at the construction site and that it is inevitable for the supervisor to proceed manually. Future studies include analyzing the quantitative impact of the key construction supervision work once digitalization is fully applied.

• Journal of the Korea Institute of Building Construction
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• v.6 no.1 s.19
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• pp.93-99
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• 2006

In the inner turn key constructions the problems of uniform types are repeatedly occurred, and, especially, many problems happen up to the stage of pre-construction(from a bid to a contract) owing to the specialties of the turn key constructions. So the claim factors of the Korean turn key constructions were abstracted through the literature searches, the site document examinations, the case studies and the interviews with the experts. When the technicians meeting the interior turn key constructions for the first time and working here now are well acquainted with only these factors, many claims will be prevented. By analysing the degree of claims by claims factors through the questionnaires to the experts about the abstracted factors and surveying the amount of the claims through the case studies, what factors exerted how much of influence on the claims was tested and analyzed. Proposing the response devices to the factors affecting the claims much led the technicians in charge of internal turn key constructions to the prevention and the proper solution of the site claims.