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A Theoretical Analysis of the Weak Shock Waves Propagating through a Bubbly Flow (기액 이상류를 전파하는 약한 충격파에 관한 이론해석적 연구)

  • Jun, Gu-Sik;Baek, Seung-Cheol;Kim, Heuy-Dong
    • Proceedings of the KSME Conference
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    • 2004.11a
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    • pp.1617-1622
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    • 2004
  • Two-phase flow of liquid and gas through pipe lines are frequently encountered in nuclear power plant or industrial facility. Pressure waves which can be generated by a valve operation or any other cause in pipe lines propagate through the two-phase flow, often leading to severe noise and vibration problems or fatigue failure of pipe line system. It is of practical importance to predict the propagation characteristics of the pressure waves for the safety design for the pipe line. In the present study, a theoretical analysis is performed to understand the propagation characteristics of a weak shock wave in a bubbly flow. A wave equation is developed using a small perturbation method to analyze the weak shock wave through a bubbly flow with comparably low void fractions. It is known that the elasticity of pipe and void fraction significantly affect the propagation speed of shock wave, but the frequency of relaxation oscillation which is generated behind the shock wave is not strongly influenced by the elasticity of pipe. The present analytical results are in close agreement with existing experimental data.

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Development of An Inspection Method for Defect Detection on the Surface of Automotive Parts (자동차 부품 형상 결함 탐지를 위한 측정 방법 개발)

  • Park, Hong-Seok;Tuladhar, Upendra Mani;Shin, Seung-Cheol
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.22 no.3
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    • pp.452-458
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    • 2013
  • Over the past several years, many studies have been carried out in the field of 3D data inspection systems. Several attempts have been made to improve the quality of manufactured parts. The introduction of laser sensors for inspection has made it possible to acquire data at a remarkably high speed. In this paper, a robust inspection technique for detecting defects in 3D pressed parts using laser-scanned data is proposed. Point cloud data are segmented for the extraction of features. These segmented features are used for shape matching during the localization process. An iterative closest point (ICP) algorithm is used for the localization of the scanned model and CAD model. To achieve a higher accuracy rate, the ICP algorithm is modified and then used for matching. To enhance the speed of the matching process, aKd-tree algorithm is used. Then, the deviation of the scanned points from the CAD model is computed.

Study on the Thermostability of Contractile Myofibrillar Proteins from Fish Species (어류의 수축성 근섬유단백질의 열안정성에 관한 연구)

  • Yang, Ryung;Hong, Sang-Pill;Shin, Wan-Chul;Song, Jae-Chul
    • Korean Journal of Food Science and Technology
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    • v.20 no.6
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    • pp.862-867
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    • 1988
  • Myofibrillar proteins were prepared from cold(pollack, salmon) and warm current fish(shark), and their thermostabilities were compared. Thermodynamic data for inactivation of myofibrillar proteins, such as D-value, Kd-value, revealed that thermostability of myofibrillar proteins from warm current fish was higher than that from cold current fish.

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Polyhedral Protein Synthesis and DNA Replication of Bombyx mori, Nuclear Polyhedrosis Virus in a B. mori Cell Line (가잠 배양세포에서 핵다각체병 바이러스의 다각체 단백질 합성과 DNA 복제)

  • 진병래;박범석
    • Journal of Sericultural and Entomological Science
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    • v.33 no.1
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    • pp.21-26
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    • 1991
  • Bombyx mori nuclear polyhedrosis virus (BmNPV) was successfully multiplied in the nuclear of BmN4 cells cultured with insect Grace's medium. By electron microscopic observation, the virons had a single nucleocapsid in an envelope. Polyhedral protein synthesis of BmNPV in BmN4 cells was detected at 18 hr p.i. and polyhedral protein was a singlepolypeptide with a M.W of 30 kd. At 48 hr p.i. polyhedra formation was observed by inverted mociroscope and electron microscope. Genome analysis of BmNPV by restriction endonucleases was not revealed the difference between virus produced in vivo and that in vitro.

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Congenital transmission of Theileria sergenti in cattle verified by immunohistochemistry (소 Theileria sergenti의 태반감염에 대한 면역세포화학적 증명)

  • Baek, Byeong-kirl;Kim, Jin-ho;Onuma, Misao
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.825-829
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    • 1997
  • The spleen and placenta from the aborted fetuses as well as lymphnodes and placenta of the corresponding dams naturally infected with T sergenti were used to localize the parasite antigens by immunohistochemical staining for the possible congenital transmission of theileriosis. Parasite-specific antigens were detected immunohistochemically by incubating the sections with specific monoclonal antibody prepared against 34KD surface antigen of T sergenti and visualized via the avidin biotin complex(ABC) method. Specific T sergenti antigen was detected in the sections of formalin or acetone-fixed fetal spleens and placenta. Similar antigens were also demonstrated in lymphnodes and placentas of the corresponding dams. It is concluded that this technique will eventually play an important role in specialized diagnostic laboratories in the verification/evaluation of congenital infection with T sergenti.

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Sequence Analysis of $\beta$-Xylosidase Gene from Bacillus stearothermophilus (Bacillus stearothermophilus $\beta$-Xylosidase 유전자의 염기 서열 결정 및 분석)

  • 오현주;최용진
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.134-142
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    • 1994
  • The neucleotide sequences of the xylA gene encoding $\beta $-xylosidase of Bacillus stearothermophilus and is its flanking regions were datermined. Three open reading frame(ORFs) were found, one of which(ORF1) appeared to code for the $\beta $-xylosidase. The 1830 base pair ORF1 encoded 609 amino acids starting from a TTG initiation codon. The molecular weight deduced from the nucleotide sequence(68 KD) was in agreement with that estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme(66 KD). The Shine-Dalgarno sequence(5'-AGGAGG-3') was found 11 bp upstream of the initiation codon. Further 15 bp upstream, there observed a potential transcription initiation signals. The putative -10 sequence(CATAAT) and -35 sequence(TTGTTA) coresponded closely to the consensus sequences for Bacillus subtilis RNA polymerase with major sigma factor. The guanine-plus-cytosine content of the coding region of the xylA gene was 56mol% while that of the third position of the codons was 63 mol%. Based on the comparison with the amino acid sequences of several other carbohydrate degrading enzymes, two conserved regions, possibly participating in the catalytic mechamism of $\beta $-xylosidase xylA, were identified in 278-298 and 329-350 regions of the translated xylA gene. The nucleotide sequence of the xylA was found to exhibit no homology to any other genes so far reproted.

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Indexing Moving Objects with Real-Time Updates (실시간 갱신을 통한 이동 객체의 색인 기법)

  • Bok Kyoung-Soo;Seo Dong-Min;Yoo Jae-Soo
    • The Journal of the Korea Contents Association
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    • v.4 no.4
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    • pp.141-152
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    • 2004
  • In this paper, we propose the index structure supporting the future position retrievals with efficiently updating continuous positions of moving objects in location based services. For reducing update costs of moving objects, our index structure directly accesses to the leaf node with moving objects using secondary index structure and performs bottom up update when node information is changed. Positions of moving objects are stored in primary index structure. In primary index structure, the split information similar to kd-tree is stored to internal node for increasing node's fanout. And the proposed index structure supports the future position retrievals using velocity of moving objects in the child node.

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Molecular Cloning of Pseudomonas sp.Inulinase Gene and its Expresstion in E. coli (Pseudomonas sp. Inulinase 유전자의 클로닝 및 Escherichia coli에서의 발현)

  • 엄수정;권영만;최용진
    • Microbiology and Biotechnology Letters
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    • v.23 no.5
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    • pp.550-555
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    • 1995
  • A strain of Pseudomonas sp. isolated from soil was shown to produce a high level of extracellular endo-inulinase. In this work, the endo-inulinase gene (inu1) of the bacterial strain was cloned into the plasmid pBR322 by using EcoRI restriction endonuclease and E. coli HB101 as a host strain. One out of 7, 000 transformants obtained from the above cloning experiment formed a clear zone around its colony on the selective medium supplemented with 2.0% inulin after a prolonged incubation at 37$\circ$C and subsequent cold shock treatment. The functional clone was found to carry a recombinant plasmid (pKMG50) with a 3.7 kb genomic insert containing the genetic information for the inulinase activity. The inulinase from E. coli HB101/pKMG50 was proved to be an endo-acting enzyme and produced constitutively in the recombinant E. coli cells. Zymogram of the enzyme from the recombinant cells with inulin substrate indicated that the molecular mass of the active protein was 190 Kd, while that of the endo-inulinase from the Pseudomonas strain was 170 Kd. This size discrepancy suggested that the inulinase from the recombinant E. coli HB101 cells might be the initial product of translation, not the mature form produced in the strain of Pseudomonas sp..

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A Database Creation and Retrival Method of Feature Descriptors for Markerless Tracking (마커리스 트래킹을 위한 특징 서술자의 데이터베이스 생성 및 검색방법)

  • Yun, Yo-Seop;Kim, Tae-Young
    • Journal of Korea Game Society
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    • v.11 no.3
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    • pp.63-72
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    • 2011
  • In this paper, we propose a novel database creation and retrieval method of feature descriptors to support real-time marker-less tracking in the augmented reality environments. Each feature descriptor is encoded by integer and multi-level database is created in order to retrieve a feature descriptor efficiently. The retrieval of a feature descriptor is performed as follows: Firstly, candidate feature descriptors are searched by traversing the multi-level database. Secondly, the euclidean distance between input feature descriptor and each candidate one is compared. The shortest one is retrieved. The proposed method is 16 ms faster than previous KD-Tree method for each feature descriptor.

Chorion Gene Expression in the Cellular Differentiation and Accumulation of Chorion Protein of Silkmoth, Bombyx mandarina I. Specific Structures of Egg-shell and Chorion Protein (한국산 멧누에 (Bombyx mandarina)에 있어서 난각유전자의 형질발현. I. 난각구조의 특이성과 Chorion 단백질)

  • 노시갑
    • Korean journal of applied entomology
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    • v.29 no.3
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    • pp.157-164
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    • 1990
  • The surface patterns and the structures of transverse section of the egg-shell of the sikmoth, Bombyx mandarina, have been described by scanning electron microscope. Three spatially differentiated cross section, called lamellar, conic pillar and cover layers, are found on the mature eg-shell. Silkmoth chorion proteins were detected more than 80 components from a single chorion by two-dimensional electrophoresis. Major protein components of the egg-shell have bee identified on the basis of their isoelectric points and molecular weights, pH 4-6 and 6-30 kd. Several protein components are found entirely or predominantly in th cover layers.

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