• Applied Microscopy
• /
• 제39권1호
• /
• pp.79-83
• /
• 2009

We report a useful method to estimate the electron dose rate which may be a decisive factor to characterize sample properties. Even though most mircoscopes have their own exposure meters, there are several practical concerns when such exposure meters are used to measure the electron dose rate: 1) Specimen should be avoided within the entire area of exposure meter; 2) beam current has to be always recorded whenever the operation mode is changed; 3) the electron dose rate can not be calculated for the beam current beyond the detectable range. To overcome these limitations, we suggest a useful method which utilize a CCD (charge coupled device) camera which is now a popular detector to obtain the final electron micrographs. We have evaluated the CCD sensitivity using the linear relationship between electron current on the exposure meter and counter ratio on the CCD camera which are built in KBSI-HVEM (high voltage electron microscope). Applying the new method, we obtained the CCD sensitivity which are approximately 0.039 counts/$e^-$ and 1.37 counts/$e^-$ for the Top-TV and the HV-GIF CCD cameras, respectively.

• Applied Microscopy
• /
• 제39권1호
• /
• pp.73-77
• /
• 2009

Contaminative artifacts such as carbonaceous materials on carbon-coated microgrids are unavoidable, which is induced by electron beam exposure inside electron microscopes. This phenomenon raise a source to produce confusing information to the samples investigated by analytical TEM, which should be alleviated as much as possible. As experimental precautions for reducing this unwanted effect, the use of $LN_2$ cooled anti-contaminator and pre-illumination of electron beam at low magnification can be helpful. Nevertheless, we should be cautious to set an illumination condition for microanalysis because the contaminative effect is dependent with the types of irradiation situations, which is well known to be a decisive factor for causing the carbonaceous artifacts. Accordingly, it is necessary that optimal illumination to minimize the contaminative effect should be selected for improving the accuracy of microanalysis. In this paper, we introduce the practical method to determine the optimal illumination condition by evaluating the contaminative effect as a function of instrumental spot size, which is directly linked with electron current density.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 추계학술대회 논문집
• /
• pp.184-187
• /
• 2005

This paper is described about the technique of ultra-precision machining for optical parts in HMD system. Machining technique for PMMA and BK7 with single point diamond turning machining is reported in this paper. The main factors influencing on the machined surface quality are discovered and regularities of machining process are drawn. The purpose of our research is to find the optimum machining conditions fur cutting of PMMA and grinding of BK7. Also, apply the SPDTM technique to the manufacturing of ultra precision optical components of HMD system. Aspheric PMMA lens without a polishing process, the surface roughness of 5 nm Ra, and the form error of ${\lambda}/2\;({\lambda}=632.8nm)$ for reference curved surface 30 mm has been required.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 춘계학술대회 논문집
• /
• pp.444-447
• /
• 2005

This paper is described about the technique of ultra-precision machining for a infrared camera aspheric mirror. A 200 mm diameter aspheric mirror was fabricated by SPDTM. Aluminum alloy as mirror substrates is known to be easily machined, but not polishable due to its ductility. Aspheric large reflector without a polishing process, the surface roughness of 5 nm Ra, and the form error of $\lambda/2\;(\lambda=632.8 nm)$ for reference curved surface 200 mm has been required. The purpose of this research is to find the optimum machining conditions for cutting reflector using A16061-T651 and apply the SPDTM technique to the manufacturing of ultra precision optical components of Al-alloy aspheric reflector.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 춘계학술대회 논문집
• /
• pp.448-451
• /
• 2005

An aspheric mirror, which requires less than $\lambda/2\;(\lambda=632.8nm)$ of form error for the $\phi$ 200mm reference curved surface, has been manufactured with an ultra-precision turning machine. We have known through several tests that the deformation patterns of the reflecting surface is related with bolting positions. In this paper the effect of main factors on deformation of a reflector is studied with a FE code. The considered factors are angular velocity, natural frequencies for a mirror, temperature increment during machining. The obtained test results are similar to the deformation shape due to the assumed temperature increment.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 춘계학술대회 논문집
• /
• pp.1566-1570
• /
• 2005

The aspherical lenses are used as optical lens of HMD optical system. The optimum cutting condition of PMMA lens sample with ultra precision SPDT, the diamond tool nose radius, the cutting speed, the feed rate, the depth of cut, and cutting fluid type are found. The demanded surface roughness 10 nm Ra, aspherical form error $1.0\;\mu{m}$ P-V for aspherical lens of optical data storage device are satisfied.

• Bulletin of the Korean Chemical Society
• /
• 제26권1호
• /
• pp.34-35
• /
• 2005

• Bulletin of the Korean Chemical Society
• /
• 제33권6호
• /
• pp.2099-2101
• /
• 2012

• 한국전자현미경학회:학술대회논문집
• /
• 한국현미경학회 2004년도 제35차 춘계학술대회
• /
• pp.69-73
• /
• 2004

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
• /
• pp.411.1-411.1
• /
• 2014

This study evaluates the utility of an antibacterial microneedle composed of green tea extract (GT) and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ${\sim}60mm^2$ with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate were sustained for 72 h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles are not generally cytotoxic to chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24 h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida) and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth in the infected skin wound sites and improved skin wound healing process in rat model.