• Journal of the Korean Society of Tobacco Science
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• v.10 no.1
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• pp.27-36
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• 1988

This experiment was tarried out to investigate the effect of plant spacing and amount of side dressing on the yield, quality and protein pattern in burley 21 and KB 101. The results obtained were summarized as follow. 1 Yield and alkaloid content are increased In high plant population and side dressing. 2 . Quality is not affected by plant population and side dressing. 3 . Nitrogen content is decreased by late growing stage. 4. The bands of burley 21 seed are fewer than KB 101 seed bands in protein pattern. 5. In protein pattern, the bands of KB 101s leave are thicker than that of burley21s leave between 18,000∼14,000 of molecular weight.

• BMB Reports
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• v.30 no.1
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• pp.33-36
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• 1997

Age-dependent deletion of mitochondrial DNA (mtDNA) was detected in mouse brain using PCR method. The size of the deleted fragment was 0.5 kb, 0.9 kb. 1.7 kb and 4.3 kb in the region between cytochrome b gene and ATPase 6 gene. The deleted fragment was increased gradually from 3-month to 22month Direct repeat sequence flanking the deletion in 0.5 kb PCR product was TAAT.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.821-825
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• 2018

A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.

• The Journal of Information Technology and Database
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• v.1 no.2
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• pp.3-24
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• 1994

기존의 데이타 모델 및 설계 방법론들은 실세계의 데이타 객체에 대해 고정된 한 측면의 모델 표현만을 허용하기 때문에 여러 측면으로 관측이 가능한 실세계 객체들의 표현에 어려움을 갖는다. 제시한 객체 중심 측면 모델(OOAM : Object-Oriented Entity Aspect Model)은 실세계의 각 객체에 대해 다수의 측면 표현을 가능하게 한 객체 중심의 데이타 모델로 데이타와 지식 표현에 유용한 모델이다. 데이타베이스 시스템과 지식베이스 시스템 중 어느 하나의 시스템이 다른 시스템의 특징을 빌리거나 통합할 수 있다면 두 시스템에게 서로 이득이 될 수 있다. 이러한 KB/DB(Knowledge Base/Data Base)의 통합은 최근에 객체 지향 개념과 연역 개념에 의해 연구가 활발히 진행되고 있다. 본 논문에서는 객체의 측면 개념을 제공하는 OOAM의 기본 개념을 보여주고 OOAM에 의해 구축되는 데이타베이스 스키마의 시맨틱을 분석하고 서술하기 위해 OOAM을 형식적으로 정의하였다. 그리고 KB/DB 통합에 관련된 연구들을 분석하고 데이타베이스에 관련된 지식의 종류를 서술한 후 OOAM을 사용하여 KB/DB 통합을 위한 지식베이스와 데이타베이스의 개발 방법론을 제시하였다.

• Journal of the Korean Institute of Landscape Architecture
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• v.30 no.1
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• pp.96-104
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• 2002

This study was conducted to investigate physical properties of soil and turfgrass wear characteristics within turfgrasses inside or outside the stadium A 1/1000 scale model Inchon world cup soccer d[me was constructed for this test. Turfgrasses planted inside and outside the model dome were; Kentucky bluegrass(KB), Kentucky bluegrass + perennial ryegrass mixture (KB+PR), Kentucky bluegrass + tall fescue + perennial ryegrass mixture (KB+TF+PR), Zoysia japonica 'Anyangjungzii'(ZA) and Zoysia japonica 'Zenith\`(ZZ). The rootzone was constructed by the multi-layer method (United States Golf Association method). Traffic on turfgrasses was treated with a 120kg roller. Surface soil hardness, soil penetration and water infiltration values on cool-season grasses(KB, KB+PR, KB+TF+PR) was found to be better for soccer play compared to zoysiagrasses(ZA, ZZ). No big differences in surface soil hardness, soil penetration and water infiltration values were found between inside and outside of the model dome. Wear damage on cool-season grasses caused by the traffic treatment was low compared to zoysiagrasses. However, there was no difference in wear damage by the traffic treatment within cool-season grasses while wear damage on ZA was higher than on ZZ within zoysiagrasses. It could be concluded that physical properties and wear characteristics on cool-season grasses were much better for soccer play than on zoysiagrasses.

• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.17-21
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• 1991

A gene coding for ${\beta}-xylosidase$ in alkalophilic Bacillus sp. YC-335 isolated from soil was cloned into Escherichia coli HB101 using plasmid pBR322. The recombinant plasmid pYK40 was isolated, and the cloned HindIII fragment was 15 kilobases (kb). To reduce the size of the inserted DNA fragment of pYK40, the 15 kb HindIII fragment was subjected to a series of subclonings. A 6 kb subfragment was found to code for ${\beta}-xylosidase$ activity, and the recombinant plasmid was named pYK44. Southern hybridization analysis revealed that the cloned gene hybridized with 3.5 kb, 1.5 kb, and 1.0 kb of HindIII cleaved chromosomal DNA from Bacillus sp. YC-335. ${\beta}-xylosidase$ activity produced by recombinant E. coli was found to be 11 times higher than that produced by Bacillus sp. YC-335. Xylan was required to induce the production of ${\beta}-xylosidase$ in Bacillus sp. YC-335.

• The Korean Journal of Pesticide Science
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• v.13 no.4
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• pp.332-335
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• 2009

Rhizo-bacteria were isolated from organic-farming soils to select antagonistic agent for controlling citrus melanose disease. Among several antagonistic bacteria, KB-401 effectively inhibited mycelial growth of several plant fungal pathogens, including the pathogen of citrus melanose, Diaporthe citri. KB-401 also inhibited spore germination of the fungal pathogen. The tip of germ tube was swollen when conidia of D. citri were co-culture with KB-401 in PD broth amended 1% glucose. KB-401 was identified as Bacillus subtilis through the investigation for physiological characters and the analysis of nucleotide sequences of 16S rDNA.

• Microbiology and Biotechnology Letters
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• v.17 no.2
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• pp.160-164
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• 1989

A 5.7Kb EcoRI fragment containing alkaline amylase gene of Bacillus sp. AL-8 obtained in the previons experiment (10) was transformed in B. subtilis via plasmid pUB110. The enzymatic proper-ties of the amylase produced by the transformants were Identical to those of the donor strain. Thus, the alkaline amylase activity from the transformant was maximum at pH 10 and 5$0^{\circ}C$. And the enzyme was very stable over the ranges of alkaline pH. In order to determine the location of the alkaline amylase gene within the 5.7Kb DNA fragment, the fragment was subcloned in E. coli. It was found that the alkaline amylase gene was located k EcoRI fragment of 3.7Kb.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.144-149
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• 1996

To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), we characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein molecular weight of 30 kDa determined by electronmicroscopy and SDS-AGE analysis, respectively. The nucleotid suquences of 876 bases including the coding region of polyhedrin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0Kb and Nco I 6.0 Kb by Southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0 Kb fragments were cloned and restriction enzyme map of these clones were determined.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.83-91
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• 1997

The vein-necrosis strain or potato virus Y (PVY-Vff) and black shank (Phytophlhora parasitica roar. nicotianae) causes severe damage on burley tobacco(Wicotiana tabacum L.) in Korea, A new burley tobacco resistance to PVY and black shank, KB 110, was developed by Korea Ginseng and Tobacco Research Institute. It was developed from the cross of Burley 21 with TC 591 in 1990, and was backrossed to Burley 21 in the following season. TC 591 has resistance to PVY and moderate resistance to race 0 of black shank, but it is susceptible to tobacco mosaic vim (TMV). KB 110 was evaluated for its resistance to PVY, TMV and black shank in the greenhouse and at fields for preliminary and performance trials. KB 110 which has secreting glandular trichomes was resistant to PVY-VN, TW and black shank. It had an erect growth habit and two more leaves per plant than that of Burley 21, and matures two to three days later. It yielded approximately 3 percent more cured leaf than the standard cultivar Burley 21, but other plant characteristics were very similar to those of Burley 21. It had acceptable standards for chemical and physical characteristics of lured leaf on regional farm test in 1995-1997. KB 110 produced average yields of good quality tobaccos and was appeared to be resistant to PVY inwhere occurrence of the virus are severe chronic at burley growing area.