• Korean Journal of Microbiology
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• v.31 no.2
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• pp.123-128
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• 1993

Genes for dinitrogenase reductase (nifH) and dinitogenase a subunit (nifD) were found to be located on 7.9 kb of EcoRI, 6.5 kb of Sail, 7.3 kb of HindlII and 4.4 kb of Pstl fragments of the genomic blot of Rhizobium sp. SNU003. a symbiotic strain from root nodule of Canavalia lineata. Nine recombinant phage nif-clones were selected from the genomic library constructed by using EMBL-3 BamHI arms of bacteriophage lambda. Among them. Rnif-6 had insert DNA of 15.3 kb. in which 7.6 kb of BamHI!SacI fragment contained nifHD region. Therefore, the 7.6 kb fragment was subcloned into pUC19 and partial restriction map was constructed. As the results, nifH and nifD were found to be located continuously on 4.5 kb of BamHI/BglIl in the genome of Rhizobium sp. SNU003 strain.

• Journal of the Korean Institute of Landscape Architecture
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• v.30 no.2
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• pp.88-94
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• 2002

This study was conducted to select suitable turfarasses for use at 2002 world cup soccer fields in Korea. A 1/1000 scale Inchon worldcup soccer dome was constructed for this research. Species and seeding rates of cool-season grasses used inside and outside the dome were Kentuck bleugrass 10g/$m^2$ (KB), Kentucky bleugrass 10g/$m^2$+ perennial ryegrass 10g/$m^2$ mixture (KB+PR) and Kentucky bleugrass 6g/$m^2$+tall fescue 14g/$m^2$+ perennial ryegrass 4g/$m^2$ mixture (KB+TF+PR). Warm-season grasses also used in this study were Zoysia japonica 'Anyangjungzii' (ZA) and Zoysia japonica 'Zenith'(ZZ) which were layed as sod. So, total 5 types of grasses were used inside and outside the dome. The rootzone was constructed by the multi-layer method(United States Golf Association method). The plots were designed by randomized block design. Cool-season grasses(KB, KB+PR, KB+TF+PR) were found to be better performers for visual rating and visual color than the zoysiagrasses(ZA, ZZ). There were no significant differences in turf performance within cool-season grasses, while ZA showed better turf performances than ZZ within zoysiagrasses. The green color was maintained for about 10 months in the col-season grasses(KB, KB+PR, KB+TF+PR) compared to about 5~6 months in the zoysiagrasses. Root length and density data revealed higher values for KB, KB+PR and KB+TF+PR compared to ZA and ZZ. Root performance of 22 was better than ZA within zoysiagrasses which was the opposite result of turf performances. There was also no significant difference between U performance inside and outside the dome. However, the decreasing tendency of turf quality inside the dome at the end of the study showed that more proper maintenance technology was needed inside the d[me. It could be concluded by this study that cool-season grasses(KB, KB+PR, KB+TF+PR) were more suitable turfgrasses than waits-season zoysiagrasses(ZA, ZZ) for use at 2002 world cup soccer fields in Korea.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.11 no.3
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• pp.179-192
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• 2013

This study is characterized the groundwater flow pattern near crystalline site of Yangbook-Myeon, Gyeong-ju City, South Korea. From the time series analyses, groundwater level could be classified into 4 types reflecting the hydrogeological characteristics and rainfall pattern. The type I (DB1-1, DB1-2) may be directly influenced by rainfall pattern. The type II (DB1-3, DB1-7, KB-1, KB-2, KB-3, KB-7, KB-14, KB-15) may be influenced by rainfall event as well as groundwater flow through water-conducting features. The type III (DB-5, DB1-6, DB2-2, KB-10, KB-11, KB-13) may be predominantly happens in the crystaline rock mass, groundwater in this type flows through the minor fracture networks rather than direct effect of rainfall event. The type IV (DB1-8, KB-9) may be influenced by irregular variation of the groundwater level due to anisotropy and heterogeneity of crystalline rock.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.115-122
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• 1993

KB103 was developed from a cross between Burley 21 and Va 528 in 1983. It was developed by haploid method derived from anther culture method. It has moderate resistance to black shank(Phytophhorn nicotianae Breda de Haan Var. nictiana Waterhouse). KB103 produced good yields and quality in 1988-1990. It flowered about four or five days later than standard variety Burley 21, and plant height, leaf width, leaves per plants were similar to those of Burley 21. In maturing and curing properties as well as in chemical constituents of cured leaves, KB 103 was very similar to that of Burley 21. Regional farm test with the breeding line, KB 8304- ADH 65 named as KB 103 in 1986, was performed for 4 crop years from 1987 at 29 locations. It was released on burley tobacco area in 1992.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3725-3728
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• 2013

Background: The mammalian target of rapamycin (mTOR) /RPS6KB1 activation has recently been implicated in tumour development, but its role in lung cancer remains unclear. The aim of this study was to explore the role of mTOR/RPS6KB1 signaling pathway in non-small-cell lung cancer (NSCLC). Methods: Immunohistochemistry was performed to assess the expression of phosphorylated mammalian target of rapamycin (p-mTOR) and its downstream ribosomal phosphorylated RPS6KB1 (p-RPS6KB1) in NSCLC patients. We also analyzed p-mTOR/p-RPS6KB1 protein expression in 45 fresh NSCLC tissues using Western blotting. Results: The expression level of p-mTOR and p-RPS6KB1 was significantly higher in NSCLC tumor specimens than that in adjacent noncancerous normal lung tissues (P<0.01). p-mTOR expression correlated with p-RPS6KB1. Furthermore, high expression level of p-mTOR or p-RPS6KB1 in NSCLC was associated with a shorter overall survival (both P<0.01). Multivariate analysis indicated high level of p-mTOR expression was an independent prognostic factor (HR=2.642, 95%CI 1.157-4.904, p=0.002). Conclusions: p-mTOR and p-RPS6KB1 could be useful prognostic markers for NSCLC.

• The KIPS Transactions:PartA
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• v.12A no.2 s.92
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• pp.127-136
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• 2005

This paper measured a proper ratio of the size of demand fetch cache $L_1$ to that of prefetch cache $L_P$ by imulation when the size of $L_1$ and $L_P$ are constant which organize space-limited level 1 cache of a single microprocessor chip. The analysis of our experiment showed that in the condition of the sum of the size of $L_1$ and $L_P$ are 16 KB, the level 1 cache organization by constituting $L_P$ with 4 KB and employing OBL and FIFO as a prefetch technique and a cache replacement policy respectively resulted in the best performance. Also, this analysis showed that in the condition of the sum of the size of $L_1$ and $L_P$ are over 32 KB, employing dynamic filtering as prefetch technique of $L_P$ are more advantageous and splitting level 1 cache by constituting $L_1$ with 28 KB and $L_P$ with 4 KB in the case of 32 KB of space are available, by constituting $L_1$ with 48 KB and $L_P$ with 16 KB in the case of 64 KB elicited the best performance.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.5
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• pp.85-93
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• 2004

This study was carried out to find out the effect of cool-season turfgrass overseeding onto zoysiagrass(Zoysia japonica L.) for extending green color and solving traffic problem during dormant periods. Overseeding rates of cool-season turfgrasses were $10g/m^2$ of Kentucky bluegrass(KB1), $20g/m^2$of Kentucky b1uegrass(KB2), $60g/m^2$ of perennial ryegrass(PR), $80g/m^2$ of Tall fescue(TF), and $10g/m^2$ of Kentucky bluegrass + $10g/m^2$ of perennial ryegrass mixture(KB+PR) onto 2m${\times}$2m(width${\times}$length) zoysiagrass plots with 3 replications.1 to 9-sca1e of visual quality and visual color were measured after treatment, Consequently cool-season turfgrass overseeding gave a good performances of extending the green periods and enhancing the turf grass quality. KB1 and KB2 were found to be excellent performances for visual quality and visual color compared to PR, TF, KB+PR and control. KB2 showed better turf performances than KB1 within Kentucky bluegrass overseedings. Turfgrass color was kept up for 10 months by overseeding of cool-season turfgrasses(KB1, KB2, PR, TP, KB+PR) compared to about 5~6 months by zoysiagrass(Control). The visual quality and visual color of PR and TF showed good performances during a green-up period of spring, but decreasing tendencies through the summer.Percent diseases data revealed also higher ratings for PR and TF compared to for KB1 and KB2.

• Journal of the Korean Society of Tobacco Science
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• v.24 no.2
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• pp.130-132
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• 2002

The new burley tobacco variety, KB 301, was developed by the cross combination ’(Ky 17 x TI 1068)F$_4$ x TC 613’. The modified pedigree breeding method was used in the selection procedures. The agronomic traits and chemical constituents of KB 301 were very similar to those of Burley 21. But KB 301 had significantly higher content of total DVT than Burley 21. KB 301 was also resistant to black shank, TMV and PVY.

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.57-64
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• 1994

Burley tobacco (Nicotiana tabacum L.) KB 108 was developed from a single cross between KB 104 and TC 591 which was developed from a cross between Burley 49 and Tobacco Introduction 1406. It was tested for its resistance to black shank, potato virus Y(PVY), TMV and agronomic characteristics under field conditions. KB 108 has resistance to tobacco mosaic virus(TMV) and necrotic strain of potato virus Y(PVY-VN) with secreting glandular trichomes. It has also moderate resistance to black shank caused by phytophthora parasitica val. Nicotianae. KB 108 has an up-right plant growth habit similar to Burley 21. It flowers about 1-2 days later than Burley 21. The leaf width and length of KB 108 are approximately 3 cm wider and longer than those of Burley 21. The yield of KB 108 was higher 4%, nearly equal in value per kg compared to Burley 21.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.141-147
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• 1993

Plasmid DNAs were detected from the mitochondrial fraction of four strains of whiterot fungus, Pleurotus ostreatus. The size of the plasmids were 10.2 and 7.2 kb in strain NFFA 2, 10.2 kb in NFFA 4001, 11.2 kb in NFFA 4501, and 10.2 and 11.2 kb in KFCC 11635. The two strains,NFFA 2ml and NFFA 2m2, which are mutant derivatives of NFFA 2, did not contain any plasmids. The cleavage by proteinase K indicated that these plasmids have DNA ends associated with proteins. In digestion with proteinase K all the plasm ids remained resistant to lambda exonuclease which hydrolyzes DNA from 5' ends and were sensitive to exonuclease III which hydrolyzes DNA from 3' ends. This suggests that the plasmids are linear double-stranded DNA and the terminal proteins are covalently linked to 5' ends of plasm ids. In order to find relationship between these plasmids, hybridization of plasm ids by each separate plasmid DNA was done. The result indicated that the plasmids can be classified into at least 3 groups. Plasmids of group I were present in all the P ostreatus. More mitochondrial plasmids were detected in P cornucopiae. P ,florida, P pulmonarius, P sajor-caju, and P spodoleucus. The size of plasmids ranged between 7.2 kb and 14 kb. All the species except P cornucopiae contained plasmids of approximately 10 kb which hybridized with the 10.2 kb plasmid (group I) of P ostreatus NFFA 2.