• Journal of Microbiology
• /
• 제43권5호
• /
• pp.406-410
• /
• 2005

In contrast to Saccharomyces cerevisiae, little is known about the kinesin-like protein (KLP) in Candida albicans. The motor domain of kinesin, or KLP, contains a subregion, which is well conserved from yeast to humans. A similarity search, with the murine ubiquitous kinesin heavy chain region as a query, revealed 6 contigs that contain putative KLPs in the genome of C. albicans. Of these, the length of an open reading (ORF) of 375 amino acids, temporarily designated CaKAR3, was noticeably short compared with the closely related S. cerevisiae KAR3 (ScKAR3) of 729 amino acids. This finding prompted us to isolate a ${\lambda}$ genomic clone containing the complete CaKAR3 ORF, and here the complete sequence of CaKAR3 is reported. CaKAR3 is a C-terminus motor protein, of 687 amino acids, encoded by a non-disrupting gene. When compared with ScKAR3, the amino terminal region of 112 amino acids was unique, with the middle part of the 306 amino acids exhibiting $25\%$ identity and $44\%$ similarity, while the remaining C-terminal motor domain exhibited $64\%$ identity and $78\%$ similarity, and have been submitted to GeneBank under the accession number AY182242.

• 미생물학회지
• /
• 제30권1호
• /
• pp.37-41
• /
• 1992

Saccharomjyces cerevisiae에서 KEM1 유전자는 세포분열시 microtubules과 spindle pole body 구조체의 새로운 기능에 관여하는 것으로 알려져 있다. KEM1과 유사하거나 연관이 있는 기능을 갖는 새로운 유전자들을 찾는 목적으로 kem1 돌연변이의 high copy suppressor 유전자, ROK1, 를 찾아냈다. ROK1은 high copy 플라스미드에 클로닝되었을 때 kem1을 suppression 하고, low copy 플라스미드에서는 suppression 하지 않는다. kem1 돌연변이의 benomyl에 대한 민감성과 Kar enhancing 표현서을 동시에 suppression 하는 두 개의 클론을 분리하였으며, 제한요소로 분석했을 때 9.0kb의 insert 를 지닌 동일한 클론이었다. 이 suppressor 유전자 ROK1의 제한지도를 작성하였고, 그 결과 KEM1 이 아닌 다른 유전자인 것으로 나타났다. Suncloning 실험으로 ROK1은 적어도 3.0kb의 기능부위를 갖음을 확인했다.

• Journal of Microbiology and Biotechnology
• /
• 제24권8호
• /
• pp.1034-1043
• /
• 2014

This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at $30{\pm}2^{\circ}C$. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight ($4.32{\pm}0.03g/l$) as well as total carotenoids ($0.783{\pm}0.002mg/g$ dry cell weight). These values were significantly higher than those for dry cell weight ($3.77{\pm}0.02g/l$) and total carotenoid content ($0.706{\pm}0.008mg/g$) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.