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Carbonate Biomineralization Using Speleothems and Sediments from Baekasan Acheon Cave (Limestone Cave) in Hwasun-gun, Jeollanam-do, South Korea (전남 화순군 백아산 아천동굴(석회동굴) 동굴생성물을 이용한 생광물화작용 연구)

  • Kim, Yumi;Seo, Hyunhee;Jo, Kyoung-nam;Jung, Dayae;Shin, Seungwon;Huh, Min;Roh, Yul
    • Journal of the Mineralogical Society of Korea
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    • v.31 no.2
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    • pp.113-121
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    • 2018
  • Baekasan Acheon cave located in Hwasun-gun, Jeollanam-do is a natural limestone cave only found in this province. In this study, the mineralogical and geochemical characteristics of speleothems collected from Baekasan Acheon cave were identified and the capability of carbonate mineral formation by aerobic microorganisms enriched from the cave and the mineralogical and geochemical characteristics of carbonate minerals formed by the microorganisms were investigated. The samples of sediments (clay) and speleothems (shelfstone and cave coral) were collected at three sites in the cave. The samples of shelfstone and cave coral were identified mainly as carbonate mineral, Mg-rich calcite, and clay minerals were composed of quartz, muscovite, and vermiculite by X-ray diffraction (XRD) analysis. To cultivate the carbonate forming microorganisms, parts of the sediment and speleothems were placed in D-1 medium containing urea, respectively, and the growth of microorganisms was observed under the aerobic condition at room temperature. The capability of carbonate mineralization of the cultured Baekasan Acheon cave microorganisms was examined through adding 1% (v/v) of the cultured microorganisms and calcium sources, Ca-acetate or Ca-lactate, into the D-1 medium. XRD analysis showed that the microorganisms cultured in cave deposits formed calcium carbonate ($CaCO_3$) under all conditions, and these microbial carbonate minerals included calcite and vaterite. The morphological characteristics and chemical composition of biologically formed minerals were observed by SEM-EDS showed various crystal forms such as rhomboid, spherical, perforated surface with Ca, C, and O of major chemical components. The existence of such microorganisms in the cave can contribute the formation of carbonate minerals, and it is likely to affect the geochemical cycles of carbon and calcium in the cave.

Suitability Classes for Italian Ryegrass (Lolium multiflorum Lam.) Using Soil and Climate Digital Database in Gangwon Province (강원도에서 토양과 기후 데이터베이스를 이용한 이탈리안 라이그라스의 재배 적지 구분)

  • Kim, Kyung-Dae;Sung, Kyung-Il;Jung, Yeong-Sang;Lee, Hyun-Il;Kim, Eun-Jeong;Nejad, Jalil Ghassemi;Jo, Mu-Hwan;Lim, Young-Chul
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.4
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    • pp.437-446
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    • 2012
  • As a part of establishing suitability classification for forage production, use of the national soil and climate database was attempted for Italian ryegrass (Lolium multiflorum Lam., IRG) in Gangwon Province. The soil data base were from Heugtoram of the National Academy of Agricultural Science, and the climate data base were from the National Center for Agro-Meteorology, respectively. Soil physical properties including soil texture, drainage, slope available depth and surface rock contents, and soil chemical properties including soil acidity and salinity, organic matter content were selected as soil factors. The crieria and weighting factors of these elements were scored. Climate factors including average daily minimum temperature, average temperature from March to May, the number of days of which average temperature was higher than $5^{\circ}C$ from September to December, the number of days of precipitation and its amount from October to May of the following year were selected, and criteria and weighting factors were scored. The electronic maps were developed with these scores using the national data base of soil and climate. Based on soil scores, the area of Goseong, Sogcho, Gangreung, and Samcheog in east coastal region with gentle slope were classified as the possible and/or the proper area for IRG cultivation in Gangwon Province. The lands with gentle or moderate slope of Cheolwon, Yanggu, Chuncheon, Hweongseong, Pyungchang and Jeongsun in west side slope of Taebaeg mountains were classified as the possible and/or proper area as well. Based on climate score, the east coastal area of Goseong, Sogcho, Yangyang, Gangreung and Samcheog could be classified as the possible or proper area. Most area located on west side of the Taebaeg mountains were classified as not suitable for IRG production. In scattered area in Chuncheon and Weonju, where the scores exceeded 60, the IRG cultivation should be carefully managed for good production. For better application of electronic maps.

Recent Progress in Air-Conditioning and Refrigeration Research : A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2012 (설비공학 분야의 최근 연구 동향 : 2012년 학회지 논문에 대한 종합적 고찰)

  • Han, Hwataik;Lee, Dae-Young;Kim, Sa Ryang;Kim, Hyun-Jung;Choi, Jong Min;Park, Jun-Seok;Kim, Sumin
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.25 no.6
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    • pp.346-361
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    • 2013
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2012. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. The conclusions are as follows : (1) The research works on thermal and fluid engineering have been reviewed as groups of fluid machinery, pipes and valves, fuel cells and power plants, ground-coupled heat pumps, and general heat and mass transfer systems. Research issues are mainly focused on new and renewable energy systems, such as fuel cells, ocean thermal energy conversion power plants, and ground-coupled heat pump systems. (2) Research works on the heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer, and industrial heat exchangers. Researches on heat transfer characteristics included the results for natural convection in a square enclosure with two hot circular cylinders, non-uniform grooved tube considering tube expansion, single-tube annular baffle system, broadcasting LED light with ion wind generator, mechanical property and microstructure of SA213 P92 boiler pipe steel, and flat plate using multiple tripping wires. In the area of pool boiling and condensing heat transfer, researches on the design of a micro-channel heat exchanger for a heat pump, numerical simulation of a heat pump evaporator considering the pressure drop in the distributor and capillary tubes, critical heat flux on a thermoexcel-E enhanced surface, and the performance of a fin-and-tube condenser with non-uniform air distribution and different tube types were actively carried out. In the area of industrial heat exchangers, researches on a plate heat exchanger type dehumidifier, fin-tube heat exchanger, an electric circuit transient analogy model in a vertical closed loop ground heat exchanger, heat transfer characteristics of a double skin window for plant factory, a regenerative heat exchanger depending on its porous structure, and various types of plate heat exchangers were performed. (3) In the field of refrigeration, various studies were executed to improve refrigeration system performance, and to evaluate the applicability of alternative refrigerants and new components. Various topics were presented in the area of refrigeration cycle. Research issues mainly focused on the enhancement of the system performance. In the alternative refrigerant area, studies on CO2, R32/R152a mixture, and R1234yf were performed. Studies on the design and performance analysis of various compressors and evaporator were executed. (4) In building mechanical system research fields, twenty-nine studies were conducted to achieve effective design of mechanical systems, and also to maximize the energy efficiency of buildings. The topics of the studies included heating and cooling, HVAC system, ventilation, renewable energy systems, and lighting systems in buildings. New designs and performance tests using numerical methods and experiments provide useful information and key data, which can improve the energy efficiency of buildings. (5) In the fields of the architectural environment, studies for various purposes, such as indoor environment, building energy, and renewable energy were performed. In particular, building energy-related researches and renewable energy systems have been mainly studied, reflecting interests in global climate change, and efforts to reduce building energy consumption by government and architectural specialists. In addition, many researches have been conducted regarding indoor environments.

Effect of $H_2O_2$ on Alveolar Epithelial Barrier Properties (폐상피세포 장벽에 대한 $H_2O_2$의 영향)

  • Suh, Duk-Joon;Cho, Se-Heon;Kang, Chang-Woon
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.3
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    • pp.236-249
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    • 1993
  • Background: Among the injurious agents to which the lung airspaces are constantly exposed are reactive species of oxygen. It has been widely believed that reactive oxygen species may be implicated in the etiology of lung injuries. In order to elucidated how this oxidant causes lung cell injury, we investigated the effects of exogenous $H_2O_2$ on alveolar epithelial barrier characteristics. Methods: Rat type II alveolar epithelial cells were plated onto tissue culture-treated polycarbonate membrane filters. The resulting confluent monolayers on days 3 and 4 were mounted in a modified Ussing chamber and bathed on both sides with HEPES-buffered Ringer solution. The changes in short-circuit current (Isc) and monolayer resistance (R) in response to the exogenous hydroperoxide were measured. To determine the degree of cellular catalase participation in protection against $H_2O_2$ injury to the barrier, experiments were repeated in the presence of 20 mM aminotriazole (ATAZ, an inhibitor of catalase) in the same bathing fluid as the hydroperoxide. Results: These monolayers have a high transepithelial resistance (>2000 ohm-$cm^2$) and actively transport $Na^+$ from apical fluid. $H_2O_2$(0-100 mM) was then delivered to either apical or basolateral fluid. Resulting indicated that $H_2O_2$ decreased Isc and R gradually in dose-dependent manner. The effective concentration of apical $H_2O_2$ at which Isc (or R) was decreased by 50% at one hour ($ED_{50}$) was about 4 mM. However, basolateral $H_2O_2$ exposure led to $ED_{50}$ for Isc (and R) of about 0.04 mM. Inhibition of cellular catalase yielded $ED_{50}$ for Isc (and R) of about 0.4 mM when $H_2O_2$ was given apically, while $ED_{50}$ for basolateral exposure to $H_2O_2$ did not change in the presence of ATAZ. The rate of $H_2O_2$ consumption in apical and basolateral bathing fluids was the same, while cellualr catalase activity rose gradually with time in culture. Conclusion: Our data suggest that basolateral $H_2O_2$ may affect directly membrane component (e.g., $Na^+,\;K^+$-ATPase) located on the basolateral cell surface. Apical $H_2O_2$, on the other hand, may be largely degraded by catalase as it passes through the cells before reaching these membrane components. We conclude that alveolar epithelial barrier integrity as measured by Isc and R are compromised by $H_2O_2$ being relatively sensitive to basolateral (and insensitive to apical) $H_2O_2$.

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Bonding efficacy of cured or uncured dentin adhesives in indirect resin (간접 레진수복시 상아질 접착제의 중합 여부에 따른 결합 효능)

  • Jang, Ji-Hyun;Lee, Bin-Na;Chang, Hoon-Sang;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.36 no.6
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    • pp.490-497
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    • 2011
  • Objectives: This study examined the effect of the uncured dentin adhesives on the bond interface between the resin inlay and dentin. Materials and Methods: Dentin surface was exposed in 24 extracted human molars and the teeth were assigned to indirect and direct resin restoration group. For indirect resin groups, exposed dentin surfaces were temporized with provisional resin. The provisional restoration was removed after 1 wk and the teeth were divided further into 4 groups which used dentin adhesives (OptiBond FL, Kerr; One-Step, Bisco) with or without light-curing, respectively (Group OB-C, OB-NC, OS-C and OS-NC). Pre-fabricated resin blocks were cemented on the entire surfaces with resin cement. For the direct resin restoration groups, the dentin surfaces were treated with dentin adhesives (Group OB-D and OS-D), followed by restoring composite resin. After 24 hr, the teeth were assigned to microtensile bond strength (${\mu}TBS$) and confocal laser scanning microscopy (CLSM), respectively. Results: The indirect resin restoration groups showed a lower ${\mu}TBS$ than the direct resin restoration groups. The ${\mu}TBS$ values of the light cured dentin adhesive groups were higher than those of the uncured dentin adhesive groups (p < 0.05). CLSM analysis of the light cured dentin adhesive groups revealed definite and homogenous hybrid layers. However, the uncured dentin adhesive groups showed uncertain or even no hybrid layer. Conclusions: Light-curing of the dentin adhesive prior to the application of the cementing material in luting a resin inlay to dentin resulted in definite, homogenous hybrid layer formation, which may improve the bond strength.

The Character of Distribution of Solar Radiation in Mongolia based on Meteorological Satellite Data (위성자료를 이용한 몽골의 일사량 분포 특성)

  • Jee, Joon-Bum;Jeon, Sang-Hee;Choi, Young-Jean;Lee, Seung-Woo;Park, Young-San;Lee, Kyu-Tae
    • Journal of the Korean earth science society
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    • v.33 no.2
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    • pp.139-147
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    • 2012
  • Mongolia's solar-meteorological resources map has been developed using satellite data and reanalysis data. Solar radiation was calculated using solar radiation model, in which the input data were satellite data from SRTM, TERA, AQUA, AURA and MTSAT-1R satellites and the reanalysis data from NCEP/NCAR. The calculated results are validated by the DSWRF (Downward Short-Wave Radiation Flux) from NCEP/NCAR reanalysis. Mongolia is composed of mountainous region in the western area and desert or semi-arid region in middle and southern parts of the country. South-central area comprises inside the continent with a clear day and less rainfall, and irradiation is higher than other regions on the same latitude. The western mountain region is reached a lot of solar energy due to high elevation but the area is covered with snow (high albedo) throughout the year. The snow cover is a cause of false detection from the cloud detection algorithm of satellite data. Eventually clearness index and solar radiation are underestimated. And southern region has high total precipitable water and aerosol optical depth, but high solar radiation reaches the surface as it is located on the relatively lower latitude. When calculated solar radiation is validated by DSWRF from NCEP/NCAR reanalysis, monthly mean solar radiation is 547.59 MJ which is approximately 2.89 MJ higher than DSWRF. The correlation coefficient between calculation and reanalysis data is 0.99 and the RMSE (Root Mean Square Error) is 6.17 MJ. It turned out to be highest correlation (r=0.94) in October, and lowest correlation (r=0.62) in March considering the error of cloud detection with melting and yellow sand.

A Comparison of Peripheral Doses Scattered from a Physical Wedge and an Enhanced Dynamic Wedge (금속쐐기와 기능강화동적쐐기의 조사야 주변부 선량 비교)

  • Park, Jong-Min;Kim, Hee-Jung;Min, Je-Soon;Lee, Je-Hee;Park, Charn-Il;Ye, Sung-Joon
    • Progress in Medical Physics
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    • v.18 no.3
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    • pp.107-117
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    • 2007
  • In order to evaluate the radio-protective advantage of an enhanced dynamic wedge (EDW) over a physical wedge (PW), we measured peripheral doses scattered from both types of wedges using a 2D array of ion-chambers. A 2D array of ion-chambers was used for this purpose. In order to confirm the accuracy of the device we first compared measured profiles of open fields with the profiles calculated by our commissioned treatment planning system. Then, we measured peripheral doses for the wedge angles of $15^{\circ},\;30^{\circ},\;45^{\circ},\;and\;60^{\circ}$ at source to surface distances (SSD) of 80 cm and 90 cm. The measured points were located at 0.5 cm depth from 1 cm to 5 cm outside of the field edge. In addition, the measurements were repeated by using thermoluminescence dosimeters (TLD). The peripheral doses of EDW were (1.4% to 11.9%) lower than those of PW (2.5% to 12.4%). At 15 MV energy, the average peripheral doses of both wedges were 2.9% higher than those at 6MV energy. At a small SSD (80 cm vs. 90 cm), peripheral dose differences were more recognizable. The average peripheral doses to the heel direction were 0.9% lower than those to the toe direction. The results from the TLD measurements confirmed these findings with similar tendency. Dynamic wedges can reduce unnecessary scattered doses to normal tissues outside of the field edge in many clinical situations. Such an advantage is more profound in the treatment of steeper wedge angles, and shorter SSD.

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Characterization of MACS Isolated Cells from Differentiated Human ES Cells (인간 배아줄기세포로부터 분화된 세포에서 MACS 방법을 이용하여 분리한 세포의 특성에 대한 연구)

  • Cho, Jae Won;Lim, Chun Kyu;Shin, Mi Ra;Bang, Kyoung Hee;Koong, Mi Kyoung;Jun, Jin Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.3
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    • pp.171-178
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    • 2006
  • Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

Taxonomical Classification and Genesis of Cheongpung Series Distributed on Diluvial Terrace (홍적대지 토양인 청풍통의 분류 및 생성)

  • Song, Kwan-Cheol;Hyun, Byung-Geun;Sonn, Yeon-Kyu;Zhang, Yong-Seon;Park, Chan-Won;Jang, Byoung-Choon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.224-229
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    • 2010
  • This study was conducted to reclassify Cheongpung series based on the second edition of Soil Taxonomy, and to dicuss the formation of Cheongpung series distributed on the diluvial terrace. Morphological properties of typifying pedon of Cheongpung series were investigated, and physico-chemical properties were analyzed according to Soil survey laboratory methods manual. The typifying pedon of Cheongpung series has red (2.5YR 4/6) silty clay loam Ap horizon (0-18 cm), red (2.5YR 4/8) clay BAt horizon (18-35 cm), red (2.5YR 4/2) cobbly clay Bt1 horizon (35-65 cm), and red (2.5YR4/6) cobbly clay Bt2 horizon (more than 65 cm). The typifying pedon has an argillic horizon from adepth of 18 to more than 65 cm, and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. It can be classified as Ultisol, not as Alfisol. It has udic soil moisture regime, and can be classified as Udult. Also that meets the requirements of Typic Hapludults. It has 35% or more clay at the particle-size control section, and have mesic soil temperature regime. Therefore Cheongpung series can be classified as fine, mesic family of Typic Hapludults, not as fine, mesic family of Ultic Hapludalfs. Cheongpung series occur on moderately elevated diluvial terrace which have relatively stable geomorphic surface. They are developed as Ultisols with clay mineral weathering, translocation of clays to accumulate in an argillic horizon, and leaching of base-forming cations from the profile for relatively long periods under humid, and temperate climates in Korea.

Optimization of Pre-treatment of Tropical Crop Oil by Sulfuric Acid and Bio-diesel Production (황산을 이용한 열대작물 오일의 전처리 반응 최적화 및 바이오디젤 생산)

  • Kim, Deog-Keun;Choi, Jong-Doo;Park, Ji-Yeon;Lee, Jin-Suk;Park, Seung-Bin;Park, Soon-Chul
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.762-767
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    • 2009
  • In this study, the feasibility of using vegetable oil extracted from tropical crop seed as a biodiesel feedstock was investigated by producing biodiesel and analysing the quality parameters as a transport fuel. In order to produce biodiesel efficiently, two step reaction process(pre-treatment and transesterificaion) was required because the tropical crop oil have a high content of free fatty acids. To determine the suitable acid catalyst for the pre-esterification, three kinds of acid catalysts were tested and sulfuric acid was identified as the best catalyst. After constructing the experimental matrix based on RSM and analysing the statistical data, the optimal pre-treatment conditions were determined to be 26.7% of methanol and 0.982% of sulfuric acid. Trans-esterification experiments of the pre-esterified oil based on RSM were carried out, then discovered 1.24% of KOH catalyst and 22.76% of methanol as the optimal trans-esterification conditions. However, the quantity of KOH was higher than the previously established KOH concentration of our team. So, we carried out supplemental experiment to determine the quantity of catalyst and methanol. As a result, the optimal transesterification conditions were determined to be 0.8% of KOH and 16.13% of methanol. After trans-esterification of tropical crop oil, the produced biodiesel could meet the major quality standard specifications; 100.8% of FAME, 0.45 mgKOH/g of acid value, 0.00% of water, 0.04% of total glycerol, $4.041mm^2/s$ of kinematic viscosity(at $40^{\circ}C$).