• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.275-280
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• 2001

Bacteriocin J105 is a proteinaceous inhibitory substance produced by Latococcus latis subsp. lactis j105 isolated from Kimchi. Bacteriocin J105 was purified to homogeneity by the pH-dependent adsorption-desorption method and reverse-phase HPLC from the culture broth of Lactococcus lactis subsp. lactis J105. Purification of bacteriocin J105 resulted in a 1.47-fold increase in the specific activity and the recovery was 1.5%. Its molecular mass measured by the electrophoretic pattern in the sodium, dodecyl sulfate polyacrylamide gel was about 3.4 kDa. It was stable at $121^{\circ}C$ for 15 min at pH between 2 and 4. However, at pH above 5, bacteriocin was rapidly inactivated. Twenty-one residues from the N-terminal portion of bacteriocin J105 were sequenced using sequence analysis of lantibiotics. Bacteriocin J105 showed significant homology with known nisin A from lactic acid bacteria.

• 한국산학기술학회논문지
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• 제21권5호
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• pp.443-450
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• 2020

본 논문은 K105A1자주포에 적용중인 발사지지대 장치에서 발생한 하강운용간 멈춤현상을 해소하기 위한 설계 개선에 관한 연구이다. K105A1자주포는 기존의 105mm 견인곡사포를 차량에 탑재하여 기존의 궤도장비보다 신속한 기동성을 확보한 무기체계로써 사격충격력 및 부하를 지지하기 위해 발사지지대가 적용되어 있다. 이러한 발사지지대는 사격 전 지면에 단단히 고정되고 사격제원 유지, 차체 자세제어 등 사격임무 수행에 중추적인 역할을 하여 성능발휘에 대한 높은 신뢰성이 요구된다. 그러나, 야전운용시험 간 발사지지대를 설치하는 중 간헐적인 멈춤현상이 발생하여 지면에 고정되지 못하는 문제가 발생하였다. 이러한 문제를 해소하기 위하여 야전에서의 재연시험 및 부품단위 분해검사를 통해 고장원인을 분석 및 검토하였다. 더불어 발사지지대의 운용개념, 작동 메커니즘 및 구성품 단위의 설계분석을 통해 근본적인 원인을 도출하고 이를 해소하고자 관련된 부품의 설계를 변경하여 발사지지대의 멈춤현상을 해소하였다. 마지막으로 설계변경사항의 효과성을 확인하고자 완성체계 상태에 적용하여 발사지지대 성능 및 사격임무 수행이 정상적으로 이루어짐을 확인 및 입증하였다. 이에 따라, 본 연구에서는 K105A1자주포 발사지지대의 성능에 대한 안정성 및 신뢰성을 확보하였으며, 본 연구가 향후 군용장비 및 유사품목 개발시 많은 도움이 될 것으로 기대된다.

• 동의신경정신과학회지
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• 제13권1호
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• pp.79-115
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• 2002

This research investigates the effect of the Crataegus pinnatifida BGE. var. major N.E. BR(CPVM) on Alzheimer's disease. Specifically, the effects of the DYHT extract on (1) $IL-1{\beta}$, IL-6, amyloid precursor proteins(APP), acetylcholinesterase(AChE), and glial fibrillary acidic protein(GFAP) mRNA of PC-12 cells treated with CTI05; (2) the AChE activity and the APP production of PC-12 cell treated with CT105; (3) the behavior; and (4) expression of $IL-1{\beta}$, $TNF-{\alpha}$, reactive oxygen species(ROS), nitrite oxide(NO); and (5) the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with CT105 were investigated. The results are as follow. 1. The CPVM extract suppressed the expression of $IL-1{\beta}$, IL-6, APP, AChE, and GFAP mRNA in PC-12 cells treated with CT105. 2. The CPVM extract suppressed the AChE activity and the production of APP significantly in PC-12 cells treated with CT105. 3. The CPVM extract group showed a significant inhibitory effect on the memory deficit for the mice with Alzheimer's disease induced by CT105 in the Morris water maze experiment. 4. The CPVM extract suppressed the over-expression of $IL-1{\beta}$, $TNF-{\alpha}$, ROS and NO in the mice with Alzheimer's disease induced by CT105. 5. The CPVM extract reduced the infarction area of hippocampus, and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by CT105. These results suggest that the CPVM extract may be effective for the prevention and treatment of Alzheimer's disease.

• 대한의생명과학회지
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• 제12권1호
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• pp.53-56
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• 2006

The suprachiasmatic nucleus (SCN) of the anterior hypothalamus is the circadian pacemaker entrained to the 24-hr day by environmental time cues. Major circadian genes such as mPeriod ($mPer1{\sim}3$) and mCryptochrome ($mCry1{\sim}2$) are actively transcribed by the action of CLOCK/BMAL heterodimers, and in turn, these are being suppressed by the mPER/mCRY complex. In the study, the locomotor activity rhythms of mPer1 Knockout (KO) mice are measured, and the expression profiles of Heat Shock Protein 105kDa (HSP 105) genes in the SCN were measured by in situ hybridization. In agreement with previous reports, the locomotor activity rhythm of mPer1 KO mice was much shorter than that of wildtype. In addition, the total bout of activity of mPer1 KO was less in comparison to control mice. The expression of HSP 105 in the SCN of mPer1 KO mice was ranged from CT6 to CT22, with a peak level at CT14, implying that the gene are under the control of circadian clock. However, the expression of HSP 105 in the SCN of wildtype could not be detected in our study. Further analysis will reveal the direct or indirect regulation by mPer1 on the expression in the SCN and the role of the gene in the circadian clock.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.956-964
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• 2017

Compactin and pravastatin are competitive cholesterol biosynthesis inhibitors of 3-hydroxy-3-methylglutaryl-CoA reductase and belong to the statin drugs; however, the latter shows superior pharmacokinetic characteristics. Previously, we reported that the bacterial P450, CYP105D7, from Streptomyces avermitilis can catalyze the hydroxylation of 1-deoxypentalenic acid, diclofenac, and naringenin. Here, we demonstrate that CYP105D7 could also catalyze compactin hydroxylation in vitro. In the presence of both bacterial and cyanobacterial redox partner systems with an NADPH regeneration system, the reaction produced two hydroxylated products, including pravastatin (hydroxylated at the C6 position). The steady-state kinetic parameters were measured using the redox partners of putidaredoxin and its reductase. The $k_m$ and $k_{cat}$ values for compactin were $39.1{\pm}8.8{\mu}M$ and $1.12{\pm}0.09min^{-1}$, respectively. The $k_{cat}/K_m$ value for compactin ($0.029min^{-1}{\cdot}{\mu}M^{-1}$) was lower than that for diclofenac ($0.114min^{-1}{\cdot}{\mu}M^{-1}$). Spectroscopic analysis showed that CYP105D7 binds to compactin with a $K_d$ value of $17.5{\pm}3.6{\mu}M$. Molecular docking analysis was performed to build a possible binding model of compactin. Comparisons of different substrates with CYP105D7 were conclusively illustrated for the first time.

• 생물정신의학
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• 제1권1호
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• pp.67-78
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• 1994

The author examined the relationship of two markers, D5S39(p105-153Ra), D5S76(p105-599Ha) of chromosome 5 and $D_2$, $D_3$ receptor genes in a Korean schizophrenic pedigree using polymerase chain reaction(PCR). The results were as follows : 1) On D5S39 locus, 5 different alleles(224/226 bp : 4 cases, 218/226 bp : 3 cases, 222/226 bp : 3 cases, 218/230 bp : 1 case, 222/224 bp : 1 case) were produced. 2) On D5S76 locus, 5 different alleles(102/112 bp : 4 cases, 94/112 bp : 3 cases, 108/112 bp 3 cases, 94/94 bp : 1 case, 102/108 bp 1 case) were produced. 3) On $D_2$ receptor gene, 3 different alleles($A_1A_2$ : 8 cases, $A_1A_1$ : 2 cases, $A_2A_2$ : cases) were produced. 4) On $D_3$ receptor gene, 2 different alleles(1/2 : 7 cases, 1/1 : 5 cases) were produced. The author had not find any specific alleles on all four loci in all pedigree nor any specific alleles in the schizophrenic patients. Though the author has not found absolute relationship between the four loci and the onset of schizophrenia, there still remains the possibilities if the more detailed and elaborated pedigree studies are done.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 A
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• pp.537-539
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• 2003

퓨즈 부착 고압 교류부하개폐기(Alternating current switch-fuse combination)는 개폐기와 한류형 퓨즈의 조합으로서 각각의 규격(개폐기는 IEC60265-1, 한류형 퓨즈는 IEC60282-1)에 따른 개발시험을 실시 한 후 휴즈부착 고압교류 부하개폐기의 규격인 IEC62271-105에 따라 개발시험이 이루어진다. 본 논문에서는 최근에 개정된 IEC62271-105의 신구 비교, 시험종류 및 시험방법에 대해서 기술하였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.105.1-105.1
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• 2001

• 천문학회보
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• 제39권2호
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• pp.105.1-105.1
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• 2014

The study on linear correlation between auxiliary channels in LIGO data has been performed by using Pearson's correlation coefficient, generating a correlation map.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2001년도 춘계학술발표회요약집
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• pp.105.1-105
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• 2001