• Journal of Biosystems Engineering
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• v.29 no.6 s.107
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• pp.487-494
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• 2004

This study was conducted to develope a fertilizer-soil incorporation band tiller for the walking cultivator. Because the mixing of soil and fertilizer in the furrow of dry-field has been done manually, several time, heavy labor and much man power were required for the job. This rotary type implement is developed to substitute this manual operation for soil-fertilizer incorporation. The results of this study are summarized as follows : 1) This implement was composed of tilling device, fertilizer application device, frame and tail wheel device. 2) The revolution of driving wheel was $11\~28\;rpm$, that of application roller was $13\~14\;rpm$ the application rate range per revolution of driving wheel was $4.43\~11.80\;g$g and the application rate range by the working speed and the opening quantity was $84.12\~557.20\;g/min$. 3) The adequate working speed was $0.20\~0.40\;m/s$ and the required minimum width of open furrow was 250 mm.

• Korean Journal of Materials Research
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• v.9 no.9
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• pp.926-931
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• 1999

The microstructure, wettability, shear strength and aging effect of Sn-3.5Ag/Cu and Alloy42 lead-frame solder joints were measured for comparison. In the case of Sn-3.5Ag/Cu, $Ag_3Sn and Cu_6Sn_5$ phases in the matrix Sn and $1~2\mu\textrm{m}$ thick $Cu_6Sn_5$ phase at the interface of solder/lead-frame were formed. In the case of Sn-3.5Agl Alloy42, only AgJSn phase of low density in the matrix Sn and $0.5~1.5\mu\textrm{m}$ thick $FeSn_2$, phase at the interface of solder/leadframe were formed. Comparing to Cu, Alloy42 showed wider area of spread and smaller contact angle, thus better wet­tability. But shear strength and ductility of Alloy 42 solder joints were only 33% and 75% of those of Cu, respectively After aging at $180^{\circ}C$ for 1 week, $\xi-Cu_3Sn$ layer on $\eta-Cu_6Sn_5$ layer was formed on Cu lead-frame, while coarsened cir­cular $Ag_3Sn$ phase in the matrix and thickened $FeSn_2$, at the interface were formed on Alloy42 lead- frame.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.51-56
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• 2007

A plant pathogenic fungus, Gibberella zeae (anamorph: Fusarium graminearum), not only generates economic losses by causing disease on cereal grains, but also leads to severe toxicosis in human and animals through the production of mycotoxins in infected plants. Here, we characterized a histidine auxotrophic mutant of G. zeae, designated Z43R1092, which was generated using a restriction enzyme-mediated integration (REMI) procedure. The mutant exhibited pleiotropic phenotypic changes, including a reduction in mycelial growth and virulence and loss of sexual reproduction. Outcrossing analysis confirmed that the histidine auxotrophy is linked to the insertional vector in Z43R1092. Molecular analysis showed that the histidine requirement of Z43R1092 is caused by a disruption of an open reading frame, designated GzHIS7. The deduced product of GzHIS7 encodes a putative enzyme with an N-terminal glutamine amidotransferase and a C-terminal cyclase domain, similar to the Saccharomyces cerevisiae HIS7 required for histidine biosynthesis. The subsequent gene deletion and complementation analyses confirmed the functions of GzHIS7 in G. zeae. This is the first report of the molecular characterization of histidine auxotrophy in G. zeae, and our results demonstrate that correct histidine biosynthesis is essential for virulence, as well as sexual development, in G. zeae. In addition, our results could provide a G. zeae histidine auxotroph as a recipient strain for genetic transformation using this new selectable marker.

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.441-449
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• 2005

Photo system II (PSII) is one of the two photosynthetic reaction centers in the chloroplast of higher plants. The chlorophyll a/b-light harvesting complex serves primarily as an antenna for PSII. We isolated a cDNA that encodes a chlorophyll a/b-binding protein (Cab) from Panax ginseng. The small subunit consists of 935 nucleotides long and has an open reading frame of 795 bp with the deduced amino acid of 265 residues (pI 5.63), 28.6 kDa. The deduced amino acid sequence matched to the previously reported Cab genes. Their degree of amino acid identity ranged from 68 to $92\%$. Phylogenetic analysis based on the amino acid residues was showed that the ginseng Cab gene was grouped with P. persica (AAC34983), A. thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), and V. radiate (AAF89205).

• Structural Engineering and Mechanics
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• v.48 no.1
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• pp.1-16
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• 2013

The seismic response of RC space frame structures with isolated footing resting on a shallow soil stratum on rock is presented in this paper. Homogeneous soil stratum of different stiffness in the very soft to stiff range is considered. Soil, footing and super structure are considered to be the parts of an integral system. A finite element model of the integrated system is developed and subjected to scaled acceleration time histories recorded during two different real earthquakes. Dynamic analysis is performed using mode superposition method of transient analysis. A parametric study is conducted to investigate the effect of flexibility of soil in the dynamic behaviour of low-rise building frames. The time histories and Fourier spectra of roof displacement, base shear and structural response quantities of the space frame on compliant base are presented and compared with the fixed base condition. Results indicate that the incorporation of soil flexibility is required for the realistic estimate of structural seismic response especially for single storey structures resting on very soft soil.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.3
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• pp.193-203
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• 2018

Capture efficiencies of commercial shrimp trawls may improve if their designs took into better account behavioral responses of wild shrimp to approaching cod-end of the trawls. Here we report results of water tunnel-based experimental studies of responses of wild California target prawns to several different near-realistic netting configurations over a range of water velocities (0.3-0.7 m/s). Netting panels were oriented at parallel to water flows (FP) on the bottom of test section, vertical (VT) or diagonal sloping backward (DG), bottom to top. Behavioral responses were recorded by video camera and analyzed frame by frame. Measured responses included rates of penetrating through netting by behavioral features and tail-flip frequencies. Frequencies of prawn passing through the nets increased with flow speed for both orientations and were higher at given speeds for sloped nets. Other behavioral features (e.g., passage head-or tail-first) also varied significantly with water velocities and netting orientation. Interactions of penetrating rates between netting orientations and flow speeds also were significantly dependent, except for prawn size. Additional studies are needed of other shrimp species and at higher water velocities more similar to actual field operations using trawls to improve size selectivity.

• Structural Engineering and Mechanics
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• v.61 no.5
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• pp.663-674
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• 2017

The determination of the fundamental period of vibration of a structure is essential to earthquake design. Current codes provide formulas for the approximate estimation of the fundamental period of earthquake-resistant building systems. These formulas are dependent only on the height of the structure or number of storeys without taking into account the presence of infill walls into the structure, despite the fact that infill walls increase the stiffness and mass of the structure leading to significant changes in the fundamental period. Furthermore, such a formulation is overly conservative and unable to account for structures with geometric irregularities. In this study, which comprises the companion paper of previous published research by the authors, the effect of the vertical geometric irregularities on the fundamental periods of masonry infilled structures has been investigated, through a large set of infilled frame structure cases. Based on these results, an attempt to quantify the reduction of the fundamental period due to the vertical geometric irregularities has been made through a proposal of properly reduction factor.

• 한국전산유체공학회:학술대회논문집
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• 2005.04a
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• pp.47-50
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• 2005

The Grid'[1] means the collaboration of computing and experimental resources in dispersed organizations by high-speed network. It has been paid much attention for an unlimited number of potential resources available and the easiness to build collaborative environments among multiple disciplines. However, the difficulty in establishing the environments and accessing and utilizing the resources has prevented application scientists from conducting Grid computing. Thus, the present study focuses on building PSE(Problem Solving Environment) which assists application researchers to easily access and utilize the Grid. The Cactus toolkit, originally developed by astrophysicists, is used as a base frame for Grid PSE. Some modules are newly developed and modified for CFD(Computational Fluid Dynamics) analysis. Simultaneously, a web portal, Grid-One portal, is built for remote monitoring/control and job migration. Cactus frame through the web portal service has been applied to various CFD problems, demonstrating that the developed PSE is valuable for large-scaled applications on the Grid.

• International Journal of Oral Biology
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• v.35 no.3
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• pp.75-81
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• 2010

The present study investigated the role of ERK in the onset of mechanical and cold allodynia in a rat model of compression of the trigeminal ganglion by examining changes in the air-puff thresholds and number of scratches following the intracisternal injection of PD98059, a MEK inhibitor. Male Sprague Dawley rats weighing between 250 and 260 g were used. Under anesthesia, the rats were mounted onto a stereotaxic frame and received 4% agar ($10\;{\mu}l$) solution to compress the trigeminal ganglion. In the control group, the animals were given a sham operation without the application of agar. Changes in behavior were examined at 3 days before and at 3, 7, 10, 14, 17, 21, 24, 30, and 40 days after surgery. Compression of the trigeminal ganglion significantly decreased the air-puff thresholds. Mechanical allodynia was established within 3 days and persisted over postoperative day 24. To evaluate cold allodynia, nociceptive scratching behavior was monitored after acetone application on the vibrissa pad of the rats. Compression of the trigeminal ganglion was found to produce significant cold allodynia, which persisted for more than 40 days after surgery. On postoperative day 14, the intracisternal administration of $1\;{\mu}g$ or $10\;{\mu}g$ of PD98059 in the rat model significantly decreased the air-puff thresholds on both the ipsilateral and contralateral side. The intracisternal administration of $10\;{\mu}g$ of PD98059 also significantly alleviated the cold allodynia, compared with the vehicle-treated group. These results suggest that central ERK plays an important role in the development of mechanical and cold allodynia in rats with compression of the trigeminal ganglion and that a targeted blockade of this pathway is a potential future treatment strategy for trigeminal neuralgia-like nociception.

• Korean Journal of Microbiology
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• v.36 no.2
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• pp.103-108
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• 2000

We have cloned and analyzed cDNA coding for non-virion (NV) protein of the m V - P R T The NV gene contained 336 bp open readmg frame and encoded a protein of 11 1 amino acids with a molecular weight of 13.2 kDa. The deduced amino acid sequence of NV of IHNVPRT was found to be 90-95% identical to those of foreign isolates of IHNV. These results indicate that NV gene of the MNV is highly conserved among &ifferent strains of THNV Northern blot analyses revealed that the levels of NV gene expression were strongly elevated after 20 h post-infection. In order to identify the role of NV in the replication of MNV in fish cells, IHNVinfected cells were treated with antisense oligonucleotides. While IHNV-PRT exposed to glycoprotein (G) antisense oligonucleotide showed severely reduced growth, the growth of virus exposed to NV antisense oligonucleotide was not affected by NV antisense oligonucleotide, which suggests that NV is not essential for replication of IHNV in fish cells.