• International Journal of Naval Architecture and Ocean Engineering
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• v.6 no.4
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• pp.1024-1040
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• 2014

The theoretical background and a numerical solution procedure for a time domain hydroelastic code are presented in this paper. The code combines a VOF-based free surface flow solver with a flexible body motion solver where the body linear elastic deformation is described by a modal superposition of dry mode shapes expressed in a local floating frame of reference. These mode shapes can be obtained from any finite element code. The floating frame undergoes a pseudo rigid-body motion which allows for a large rigid body translation and rotation and fully preserves the coupling with the local structural deformation. The formulation relies on the ability of the flow solver to provide the total fluid action on the body including e.g. the viscous forces, hydrostatic and hydrodynamic forces, slamming forces and the fluid damping. A numerical simulation of a flexible barge is provided and compared to experiments to show that the VOF-based flow solver has this ability and the code has the potential to predict the global hydroelastic responses accurately.

• Structural Engineering and Mechanics
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• v.41 no.1
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• pp.1-24
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• 2012

A reinforced concrete (RC) framed structure detailed according to non-seismic detailing provisions as per Indian Standard was tested on shake table under dynamic loads. The structure had 3 main storeys and an additional storey to simulate the footing to plinth level. In plan the structure was symmetric with 2 bays in each direction. In order to optimize the information obtained from the tests, tests were planned in three different stages. In the first stage, tests were done with masonry infill panels in one direction to obtain information on the stiffness increase due to addition of infill panels. In second stage, the infills were removed and tests were conducted on the structure without and with tuned liquid dampers (TLD) on the roof of the structure to investigate the effect of TLD on seismic response of the structure. In the third stage, tests were conducted on bare frame structure under biaxial time histories with gradually increasing peak ground acceleration (PGA) till failure. The simulated earthquakes represented low, moderate and severe seismic ground motions. The effects of masonry infill panels on dynamic characteristics of the structure, effectiveness of TLD in reducing the seismic response of structure and the failure patterns of non-seismically detailed structures, are clearly brought out. Details of design and similitude are also discussed.

• Structural Engineering and Mechanics
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• v.45 no.6
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• pp.803-819
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• 2013

This paper addresses two main issues relevant to the structural assessment of buildings subjected to explosions. The first issue regards the robustness evaluation of steel frame structures: a procedure is provided for computing "robustness curves" and it is applied to a 20-storey steel frame building, describing the residual strength of the (blast) damaged structure under different local damage levels. The second issue regards the precise evaluation of blast pressures acting on structural elements using Computational Fluid Dynamic (CFD) techniques. This last aspect is treated with particular reference to gas explosions, focusing on some critical parameters (room congestion, failure of non-structural walls and ignition point location) which influence the development of the explosion. From the analyses, it can be deduced that, at least for the examined cases, the obtained robustness curves provide a suitable tool that can be used for risk management and assessment purposes. Moreover, the variation of relevant CFD analysis outcomes (e.g., pressure) due to the variation of the analysis parameters is found to be significant.

• Journal of Korean Ophthalmic Optics Society
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• v.9 no.1
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• pp.125-134
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• 2004

By applying the test methods of "Protection Eyewear Standard (KS P 8147)", "Glasses Frame Standard (KS G 3306)" and "Lens Standard for Sunglasses (KS P 4404)", this study analyzed optical functions of sunglasses now being sold in a road shop (A shop), a fashion goods store (B shop), a shop of highway rest area (C shop) and an optical shop (D shop). When compared with sunglasses (UV transmittance rate 8.8%) of a road shop, UV Block effects of sunglasses (UV transmittance rate 0.7%) of an optical shop showed good results. In addition, the average surface resistance against abrasion for sunglasses which was sold at an optical shop was 1.4% that was much higher than that of sunglasses of a road shop(2.2%).

• Proceedings of the Korean Information Science Society Conference
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• 2007.06b
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• pp.344-349
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• 2007

무선 통신 환경에서 실시간 화상 통신을 위해 제안된 3G-324M 프로토콜은 회선 교환 통신을 사용함으로 인해 제한된 대역폭을 이용해야만 하므로 전송 시 여러 가지 제약이 발생하게 된다. 특히 송수신의 스케줄링을 효율적으로 하지 못할 경우 H.223 프로토콜에서 매 타임 슬롯마다 전송 가능한 최대 크기의 데이터를 전송 버퍼에 채워주지 못해 전송 지연 시간이 발생하게 된다. 본 논문에서는 이러한 문제들을 해결하기 위한 통신 스케줄링 기법을 제시한다. 이 통신 스케줄링 기법은 화상 프레임의 종류에 따라 크기가 크게 변한다는 사실에 기초해 H.223 프로토콜의 실행 비율을 임시적으로 증가시켜 지연시간이 늘어나는 것을 최소화하고 이로 인해 내부 버퍼 사용량을 줄일 수 있도록 한다. 또 수신 버퍼에 처리해야 할 데이터가 많은 경우, 임시로 H.223 프로토콜의 실행 비율을 증가시켜 불필요한 수신 지연 시간이 발생하지 않도록 한다. 실험은 내부 버퍼의 사용량은 제안한 통신 스케줄링 기법이 다른 통신 스케줄링 기법들에 비해 효율적으로 관리 되며 패킷의 손실률, 수신 단말기에서의 지연이 줄어드는 것을 보여준다.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.577-586
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• 2019

The engineered Aspergillus oryzae has a high NADPH demand for xylose utilization and overproduction of target metabolites. Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) is one of two key enzymes in the oxidative part of the pentose phosphate pathway, and is also the main enzyme involved in NADPH regeneration. The open reading frame and cDNA of the putative A. oryzae G6PDH (AoG6PDH) were obtained, followed by heterogeneous expression in Escherichia coli and purification as a his6-tagged protein. The purified protein was characterized to be in possession of G6PDH activity with a molecular mass of 118.0 kDa. The enzyme displayed maximal activity at pH 7.5 and the optimal temperature was $50^{\circ}C$. This enzyme also had a half-life of 33.3 min at $40^{\circ}C$. Kinetics assay showed that AoG6PDH was strictly dependent on $NADP^+$ ($K_m=6.3{\mu}M$, $k_{cat}=1000.0s^{-1}$, $k_{cat}/K_m=158.7s^{-1}{\cdot}{\mu}M^{-1}$) as cofactor. The $K_m$ and $k_{cat}/K_m$ values of glucose-6-phosphate were $109.7s^{-1}{\cdot}{\mu}M^{-1}$ and $9.1s^{-1}{\cdot}{\mu}M^{-1}$ respectively. Initial velocity and product inhibition analyses indicated the catalytic reaction followed a two-substrate, steady-state, ordered BiBi mechanism, where $NADP^+$ was the first substrate bound to the enzyme and NADPH was the second product released from the catalytic complex. The established kinetic model could be applied in further regulation of the pentose phosphate pathway and NADPH regeneration of A. oryzae to improve its xylose utilization and yields of valued metabolites.

• Smart Structures and Systems
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• v.21 no.4
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• pp.521-535
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• 2018

The main purpose of this paper is to predict missing absolute out-of-plane displacements and failure limits of infill walls by artificial neural network (ANN) models. For this purpose, two shake table experiments are performed. These experiments are conducted on a 1:1 scale one-bay one-story reinforced concrete frame (RCF) with an infill wall. One of the experimental models is composed of unreinforced brick model (URB) enclosures with an RCF and other is composed of an infill wall with bed joint reinforcement (BJR) enclosures with an RCF. An artificial earthquake load is applied with four acceleration levels to the URB model and with five acceleration levels to the BJR model. After a certain acceleration level, the accelerometers are detached from the wall to prevent damage to them. The removal of these instruments results in missing data. The missing absolute maximum out-of-plane displacements are predicted with ANN models. Failure of the infill wall in the out-of-plane direction is also predicted at the 0.79 g acceleration level. An accuracy of 99% is obtained for the available data. In addition, a benchmark analysis with multiple regression is performed. This study validates that the ANN-based procedure estimates missing experimental data more accurately than multiple regression models.

• Structural Engineering and Mechanics
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• v.39 no.4
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• pp.499-520
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• 2011

The effectiveness of a technique for the repair of reinforced concrete members in combination with a technique for the repair of masonry walls of infilled frames, damaged due to cyclic loading, is experimentally investigated. Three single - story, one - bay, 1/3 - scale frame specimens are tested under cyclic horizontal loading, up to a drift level of 4%. One bare frame and two infilled frames with weak and strong infills, respectively, have been tasted. Specimens have spirals as shear reinforcement. The applied repair technique is mainly based on the use of thin epoxy resin infused under pressure into the crack system of the damaged RC joint bodies, the use of a polymer modified cement mortar with or without a fiberglass reinforcing mesh for the damaged infill masonry walls and the use of CFRP plates to the surfaces of the damaged structural RC members, as external reinforcement. Specimens after repair, were retested in the same way. Conclusions concerning the effectiveness of the applied repair technique, based on maximum cycles load, loading stiffness, and hysteretic energy absorption capabilities of the tested specimens, are drawn and commented upon.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.06a
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• pp.67-86
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• 2001

A strain producing strongly fibrinolytic enzyme was isolated from soil and was identified to be Bacillus subtilis by biochemical and physiological characterization. The optimal culture conditions for the production of fibrinolytic enzyme was determined to be 1.0% tryptone, 1.5% soluble starch, 0.5% Peptone, 0.5% NaCl, $(NH_{4})_{3}PO_4.3H_{2}O, and MgSO_{4}.7H_{2}O.$ Initial pH and temperature were pH 8.0 and $30^{\circ}C$ , respectively, The highest enzyme production was observed at 30 hours of cultivation at $30^{\circ}C$ The fibrinolytic enzyme was purified to homogeneity by DEAE Sephadex A-50 ion exchange column chromatography, 70% ammonium sulfate precipitation, Sephadex G-200 and G-75 gel filtration column chromatography. The molecular weight of the purified enzyme was 28,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A gene encoding the fibrinolytic enzyme was cloned into a plasmid vector pBluescript, transforming E.coli XL-1 Blue. The clone was able to degrade fibrin, This indicated that the gene could encode a fibrinolytic enzyme. The nucleotide sequence of the 2.7 kb insert was determined in both direction. One open reading frame composed of 1023 nucleotides was found to be a potential protein coding region. There was the putative Shine-Dalgano sequence and TATA box upstream of the open reading frame. The homology search data in the genome database showed that both the 2.7 kb insert and 1 kb open reading frame carried no significance in the nucleotide sequence of known fibrinolytic enzyme from Bacillus serovars. The recombinant cell harboring the novel gene involved in fibrinolysis was subjected to protein purification. The molecular mass of the purified fibrinolytic enzyme was determined to be 31864 Dalton, which was highly in accordance with the molecular mass(33 kDa) of the fibrinolytic gene deduced from the insert. The fibrinolytic enzyme was Purified 50.5 folds to homogeneity in overall yield of 10.7% by DEAE Sephadex A-50 ion exchange, 85% ammonium sulfate precipitation, Sephadex G-50, Superdex 75 HR FPLC gel filtration. In conclusion, a novel fibrinolytic gene from Bacillus subtilis was identified and characterized by cloning a genomic library of Bacillus subtilis into pBleuscript. For the soybean fermented by this strain, it is found that there increased assistant protein about 20% compared to the soybean not fermented and increased about 30% according to amino acid analysis and, in particular, essential amino acid increased about 40%. When keeping this fermented soybean powder at room temperature for about 70days, it showed very high stability maintaining almost perfect activity and, therefore, it gave us great suggestion its possibility of development as a new functional food.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.5
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• pp.62-73
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• 2010

In this paper we proposed a candidate algorithm on G.711.1 and G.722 superwideband extension codec which is under standardization by ITU-T. The proposed codec not only provides an interoperable bitstream with ITU-T G.711.1 and G.722, but also encodes a superwideband signal with a bandwidth of 50-14,000 Hz using superwideband extension layer. The candidate codec consists of a core layer to provide an interoperability with conventional wideband codecs and superwideband extension layer using linear prediction-based sinusoidal coding. The proposed extension codec operates on 5ms frame and provides four superwideband bitrates of 64, 80, 96, and 112 kbit/s depending on the core codec. Since the resulting bitstream has an embedded structure, it can be converted into core bitstream by simple truncation without transcoding. The proposed codec has a short algorithmic delay and low complexity and passed the qualification test of G.711.1 and G.722 superwideband extension codec performed by ITU-T.