• 제목/요약/키워드: K-ABC

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Comparison of Endonuclease-Sensitive Sites by T4 Endonuclease V and UvrABC Nuclease Treatments Followed by Formamide or Sodium Hydroxide Denaturation

  • Chang, Yung-Jin
    • BMB Reports
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    • 제31권4호
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    • pp.405-408
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    • 1998
  • Endonuclease-sensitive sites detected by T4 endonuclease V or UvrABC nuclease treatments were compared in the dihydrofolate reductase gene of UV-irradiated Chinese hamster ovary B-11 cells. The number of endonuclease-sensitive sites detected by T4 endonuclease V treatment followed by NaOH denaturation was twice that of formamide denaturation. Repeated treatment of damaged genomic DNA with T4 endonuclease V resulted in no further increase in the number of endonuclease-sensitive sites detected. The numbers of endonuclease-sensitive sites detected by UvrABC nuclease using each denaturation condition were similar. Sequential treatment with the two endonucleases using formamide denaturation resulted in twice the number of endonuclease-sensitive sites detected by treatment of each nuclease alone. Due to a lack of AP endonuclease activity these results suggest the presence of T4 endonuclease V-sensitive sites which could be complemented by alkaline gel separation or by UvrABC nuclease treatment.

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Hydrolytic Dechlorination of 4-Chlorobenzoate Specified by fcbABC of Pseudomonas sp. DJ -12

  • Chae, Jong-Chan;Ahn, Kyung-Joon;Kim, Chi-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제8권6호
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    • pp.692-695
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    • 1998
  • Pseudomonas sp. DJ-12 was able to degrade 4-chlorobenzoate by hydrolytic dechlorination to produce 4-hydroxybenzoate and chloride ion. The fcbABC genes responsible for the hydrolytic dechlorination were cloned from the chromosomal DNA of the organism. The genes were found to be organized in the order fcbB-fcbA-fcbC, but there was an intergenic space between the fcbA and fcbC genes.

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Modeling of Co(II) adsorption by artificial bee colony and genetic algorithm

  • Ozturk, Nurcan;Senturk, Hasan Basri;Gundogdu, Ali;Duran, Celal
    • Membrane and Water Treatment
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    • 제9권5호
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    • pp.363-371
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    • 2018
  • In this work, it was investigated the usability of artificial bee colony (ABC) and genetic algorithm (GA) in modeling adsorption of Co(II) onto drinking water treatment sludge (DWTS). DWTS, obtained as inevitable byproduct at the end of drinking water treatment stages, was used as an adsorbent without any physical or chemical pre-treatment in the adsorption experiments. Firstly, DWTS was characterized employing various analytical procedures such as elemental, FT-IR, SEM-EDS, XRD, XRF and TGA/DTA analysis. Then, adsorption experiments were carried out in a batch system and DWTS's Co(II) removal potential was modelled via ABC and GA methods considering the effects of certain experimental parameters (initial pH, contact time, initial Co(II) concentration, DWTS dosage) called as the input parameters. The accuracy of ABC and GA method was determined and these methods were applied to four different functions: quadratic, exponential, linear and power. Some statistical indices (sum square error, root mean square error, mean absolute error, average relative error, and determination coefficient) were used to evaluate the performance of these models. The ABC and GA method with quadratic forms obtained better prediction. As a result, it was shown ABC and GA can be used optimization of the regression function coefficients in modeling adsorption experiments.

Isolation and characterization of BrMDR1 a novel MDR-type ATP-binding cassette (ABC) transporter in Brassica rapa L.

  • Lee, Sun-Yong;Jung, Yu-Jin;Kang, Kwon-Kyoo
    • 한국자원식물학회지
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    • 제22권3호
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    • pp.273-280
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    • 2009
  • A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

융합 인공벌군집 데이터 클러스터링 방법 (Combined Artificial Bee Colony for Data Clustering)

  • 강범수;김성수
    • 산업경영시스템학회지
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    • 제40권4호
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    • pp.203-210
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    • 2017
  • Data clustering is one of the most difficult and challenging problems and can be formally considered as a particular kind of NP-hard grouping problems. The K-means algorithm is one of the most popular and widely used clustering method because it is easy to implement and very efficient. However, it has high possibility to trap in local optimum and high variation of solutions with different initials for the large data set. Therefore, we need study efficient computational intelligence method to find the global optimal solution in data clustering problem within limited computational time. The objective of this paper is to propose a combined artificial bee colony (CABC) with K-means for initialization and finalization to find optimal solution that is effective on data clustering optimization problem. The artificial bee colony (ABC) is an algorithm motivated by the intelligent behavior exhibited by honeybees when searching for food. The performance of ABC is better than or similar to other population-based algorithms with the added advantage of employing fewer control parameters. Our proposed CABC method is able to provide near optimal solution within reasonable time to balance the converged and diversified searches. In this paper, the experiment and analysis of clustering problems demonstrate that CABC is a competitive approach comparing to previous partitioning approaches in satisfactory results with respect to solution quality. We validate the performance of CABC using Iris, Wine, Glass, Vowel, and Cloud UCI machine learning repository datasets comparing to previous studies by experiment and analysis. Our proposed KABCK (K-means+ABC+K-means) is better than ABCK (ABC+K-means), KABC (K-means+ABC), ABC, and K-means in our simulations.

Hybrid artificial bee colony-grey wolf algorithm for multi-objective engine optimization of converted plug-in hybrid electric vehicle

  • Gujarathi, Pritam K.;Shah, Varsha A.;Lokhande, Makarand M.
    • Advances in Energy Research
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    • 제7권1호
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    • pp.35-52
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    • 2020
  • The paper proposes a hybrid approach of artificial bee colony (ABC) and grey wolf optimizer (GWO) algorithm for multi-objective and multidimensional engine optimization of a converted plug-in hybrid electric vehicle. The proposed strategy is used to optimize all emissions along with brake specific fuel consumption (FC) for converted parallel operated diesel plug-in hybrid electric vehicle (PHEV). All emissions particulate matter (PM), nitrogen oxide (NOx), carbon monoxide (CO) and hydrocarbon (HC) are considered as optimization parameters with weighted factors. 70 hp engine data of NOx, PM, HC, CO and FC obtained from Oak Ridge National Laboratory is used for the study. The algorithm is initialized with feasible solutions followed by the employee bee phase of artificial bee colony algorithm to provide exploitation. Onlooker and scout bee phase is replaced by GWO algorithm to provide exploration. MATLAB program is used for simulation. Hybrid ABC-GWO algorithm developed is tested extensively for various values of speeds and torque. The optimization performance and its environmental impact are discussed in detail. The optimization results obtained are verified by real data engine maps. It is also compared with modified ABC and GWO algorithm for checking the effectiveness of proposed algorithm. Hybrid ABC-GWO offers combine benefits of ABC and GWO by reducing computational load and complexity with less computation time providing a balance of exploitation and exploration and passes repeatability towards use for real-time optimization.

Activities-Specific Balance Confidence(ABC)척도에 대한 라쉬분석의 적용 (Application of Rasch Analysis to the Activities-Specific Balance Confidence (ABC) Scale)

  • 황수진;이충휘;박소연
    • 한국전문물리치료학회지
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    • 제14권1호
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    • pp.37-45
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    • 2007
  • This study was designed to examine, applying Rasch analysis based on item response theory, the questionnaires of the Activities-Specific Balance Confidence (ABC) scale for the elderly. The subjects were 99 institutional older adults and clients of social welfare facilities. The subjects (17 men, 72 women) ranged in age from 65 to 94 years (mean age 76.5 yrs). The Winsteps software was used to assess whether the ABC scale fits the Rasch model, to estimate the score and to refine the rating scale. The results are as follows. Twenty-two subjects were excluded as misfit persons. Four items were found to be misfits and the order of difficulty of the remaining 12 items was rearranged. Their balance confidence is indicated by -.64~1.12 logit, and the transformation formula is score=[(logit score+2.76)/(2.76+3.48)]${\times}$100. The most difficult item was "Walk outside in icy sidewalks" and the easiest item was "Walk around house." In conclusion, the ABC scale for the elderly has been proven reliable and valid. Therefore, it is expected to be used as an effective examination tool for treatment planning and screening for older adults.

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OFDM시스템에서 블록코딩을 이용한 PAR감소에 대한 연구 (PAR reduction using Adaptive Block Coding in the OFDM system)

  • 김태한;김영수
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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    • pp.2745-2754
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    • 2011
  • In this paper, we propose a Peak to Average power Ratio(PAR) reduction algorithm by using the Adaptive Block Coding(ABC). The problem of a PAR due to each sub-carrier overlap phenomenon in the OFDM (Orthogonal Frequency Division Multiplexing) system causes the an increasing in the complexity of the A/D converter in the transmitter and a reduction in the efficiency of the amplifier. The proposed ABC algorithm selects the block coding which provides the lowest PAR value based on the various check bit position. It is shown that a PAR of the ABC algorithm can be reduced from 8.35dB to 6.02dB in 16 sub-carriers compared to theconventional block coding scheme. We also extended the ABC algorithm to a larger number of sub-carriers and estimated their PAR values.

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NIC과 연계된 산부인과 환자 간호중재에 대한 한국 건강보험 수가체계와 미국 ABC 코드체계와의 수가 비교 분석 (Korean and United States: Comparison of Costs of Nursing Interventions)

  • 홍성정;이은주
    • 성인간호학회지
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    • 제24권4호
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    • pp.358-369
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    • 2012
  • Purpose: This study was performed to compare the costs of nursing interventions implemented for the obstetrical and gynecological patients using Korean Reimbursement System and ABC codes system developed in the US for costing out interventions performed by health care professional. Methods: First, the narrative data on nursing interventions were extracted from electronic medical record system of a tertiary university and mapped with Nursing Intervention Classification (NIC) by two researchers until 100% consent was reached. Narrative nursing interventions mapped with NIC were then remapped with ABC codes system using the electronic program developed in the research. The mapping data were analyzed with real numbers, frequency, percentage, mean, and standard deviation. Results: More nursing interventions were mapped with ABC codes than Korean reimbursement system. Total of 97 different types of narrative interventions could be mapped with NIC, 43 NIC interventions could be reimbursed by ABC code but only 16 NIC interventions were reimbursed by Korea Reimbursement System. Conclusion: Korean medical insurance fee system needs amendment to include more comprehensively interventions performed by nurses which are very important to patient outcomes. Further study is needed to develop strategies to costing out nursing interventions.

A Study on the Predictive Causal Model of Codependency for introducing Implications in Family Welfare Policy - Basing on the application of Triple ABC-X Model -

  • Ju, Sunyoung;Kweon, Seong-Ok;Park, Hwieseo
    • 한국컴퓨터정보학회논문지
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    • 제22권3호
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    • pp.139-145
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    • 2017
  • The purpose of this study is to establish a predictive causal model of codependency that is a main issue of family problem on the base of Triple ABC-X model which is a kind of family stress model. For the purpose of this study, we reviewed the concept and characteristics of codependency, affecting factors of codependency, and then reviewed the basic concept and logic of Triple ABC-X Model as theoretical viewpoint for the purpose of establishing a predictive causal model of codependency. We established it through examining main variables of codependency from Triple ABC-X Model. Main ingredients of the predictive causal model include boundary ambiguity, internal working model, internal and external locus of control, self-regard, social support, individual maladjustment etc. We established a predictive model of codependency basing on logic inferences among the variables. This study is expected to be used basic data to introduce some implications and for hereafter research.