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검색결과 755,411건 처리시간 0.272초

무 자엽과 하배축에서 저온과 ABA처리로 유도된 중탕에 강한 단백질 분석 (Induction of Boiling Stable Proteins by Cold and ABA Treatment in Radish Cotyledon and Hypocotyl)

  • 조봉희
    • 분석과학
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    • 제13권3호
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    • pp.346-350
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    • 2000
  • 백경 가을무 자엽은 선천적으로 53kDa과 29kDa인 중탕에 강한 단백질을 소유하고 있다. 저온 스트레스를 주면 중탕에 강한 36kDa과 16.5kDa 단백질이 새로 유도되었고, 53kDa과 29kDa의 단백질은 더 증가되었다. 중탕에 강한 53kDa 단백질은 자엽을 제거한지 2시간 이내에 변성되었다. 백경 가을무 하배축에서는 자엽에서와 같이 선천적으로 중탕에 강한 53kDa 단백질이 존재하고, 스트레스를 받으면 중탕에 강한 24kDa과 18kDa 단백질이 새로 유도되었다. 장춘대형 봄무 자엽에서는 저온과 ABA 스트레스를 주면 중탕에 강한 53kDa 단백질이 유도되었고, 25kDa과 23kDa 단백질이 증가되어 백경 가을무와 장춘대형 봄무에서 스트레스에 대항하여 유도되는 중탕에 강한 단백질이 다름을 알 수 있었다. 중탕에 강한 25kDa과 23kDa 단백질의 유도는 cycloheximide에 의해 방해 되었다. 22kDa 단백질은 ABA 처리로 사라졌다가 cycloheximlde의 처리로 다시 증가되어, cycloheximide는 생체 내 단백질의 분해에도 관여하는 것으로 보인다. 장춘대형 봄무의 하배축에서 스트레스로 유도되는 단백질의 양상은 백경 가을무 하배축에서 유도되는 단백질의 양상과 유사하였다.

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Spatial Optical Modulator (SOM);Samsung's Light Modulator for the Next Generation Laser Display

  • Yun, Sang-Kyeong;Song, Jong-Hyeong;Lee, Tae-Won;Yeo, In-Jae;Choi, Yoon-Joon;Lee, Yeong-Gyu;An, Seung-Do;Han, Kyu-Bum;Victor, Yurlov;Park, Heung-Woo;Park, Chang-Su;Kim, Hee-Yeoun;Yang, Jeong-Suong;Cheong, Jong-Pil;Ryu, Seung-Won;Oh, Kwan-Young;Yang, Haeng-Seok;Hong, Yoon-Shik;Hong, Seok-Kee;Yoon, Sang-Kee;Jang, Jae-Wook;Kyoung, Je-Hong;Lim, Ohk-Kun;Kim, Chun-Gi;Lapchuk, Anatoliy;Ihar, Shyshkin;Lee, Seung-Wan;Kim, Sun-Ki;Hwang, Young-Nam;Woo, Ki-Suk;Shin, Seung-Wan;Kang, Jung-Chul;Park, Dong-Hyun
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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    • pp.551-555
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    • 2006
  • A new type of diffractive spatial optical modulators, named SOM, has been developed by Samsung Electro-Mechanics for projection display and other applications. A laser display in full HD format $(1920{\times}1080)$ was successfully demonstrated by using prototype projection engines having SOM devices, signal processing circuits, and projection optics.

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치어기 조피볼락 사료내 구기자 첨가효과 (Effects of Dietary Kugija Lycium chinense Supplementation on Juvenile Korean Rockfish Sebastes schlegeli)

  • 임동규;유광열;신동건;김종응;배준영;배승철;이정열
    • 한국수산과학회지
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    • 제42권3호
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    • pp.250-256
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    • 2009
  • A 8-week feeding trial was conducted to investigate the effects of dietary supplementation of Kugija (Lycium chinense) on the growth and immunological response in juvenile Korean rockfish (Sebastes schlegeli). Six experimental diets were supplemented with Kugija at 0, 0.1, 0.5 1.0, 3.0 and 5.0% ($K_0,\;K_{0.1},\;Ko_{0.5},\;K_{1.0},\;K_{3.0},\;K_{5.0}$) on a dry-matter basis. After 2 weeks, triplicate groups of 30 fish initially averaging 3.36$\pm$0.2 g (mean$\pm$SD) were randomly distributed into the aquarium and were fed one of the experimental diets for 8 weeks. By the end of the 8-week feeding trial, fish fed the $K_{0.5}$ and $K_{1.0}$ diets exhibited a higher weight gain and specific growth rate than fish fed $K_0$ and $K_{0.1}$ diets (P<0.05). Feed efficiency of fish fed the $K_{0.5}$ diet showed significant higher value than that of fish fed the $K_0$, $K_{3.0}$ and $K_{5.0}$ diets (P<0.05). Hepatosomatic index of fish fed the $K_{0.5}$ diet was significantly higher than that of fish fed the $K_{0.1}$ and $K_{5.0}$ diets (P<0.05). Hematocrit of fish fed the $K_{0.5},\;K_{1.0}$ and $K_{5.0}$ diets was significantly higher than that of fish fed the $K_0$ diet (P<0.05). Glutamic oxaloacetic transaminase of fish fed the $K_{0.5},\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets was significantly lower than in fish fed the other diets (P<0.05). Glutamic pyruvic transaminase activity of fish fed the $K_{3.0}$ diet was significantly lower than those of fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). However, there was no significant difference in the activity of the transaminase in fish fed the $K_{0.5}\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets. Lysozyme activity of fish fed the $K_{0.5}$ and $K_{1.0}$ diets was significantly higher than that in fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). Respiratory burst activity of fish fed the $K_{3.0}$ diet was significantly higher than those of fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). However, there was no significant difference in respiratory burst activity of fish fed the $K_{0.5}\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets. Fish fed Kugija showed lower early mortality than fish fed a Kugija-free diet upon challenge with Vibrio ordalii. The results suggest that feeding of Kugija (Lycium chinense) enhances growth, non-specific immunity and disease resistance in juvenile Korean rockfish.

Isolation and Characterization of Two Mosquitocidal Bacillus thuringien- sis Strains Belonging to subsp. kurstaki and subsp. aizawai

  • Roh, Jong-Yul;Li, Ming-Shun;Chang, Jin-Hee;Shim, Hee-Jin;Jin, Byung-Rae;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • 제3권1호
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    • pp.19-23
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    • 2001
  • Two B. thuringiensis strains, which possess mosquitocidal activities, were isolated from Korean soil samples and named K-1205-1 and K-1381-1. Serological studies indicated that K-1205-1 and K-1381-1 belonged to B. thuringiensis subsp. kurstaki (H3a3b3c) and subsp. aizawai (H7), respectively. K-1205-1 produced typical bipyramidal parasporal inclusions, but K-1381-1 produced irregular bipyramidal shape. Total plasmid DNA patterns analysis shewed that K-1205-1 and K- 1381-1 were different from their reference strains, subsp. kurstaki and subsp. aizawai, respectively, in high molecules, whereas their crystal protein patterns showed no difference. The cry gene contents of K-1205-1 and K-1381-1 were identical with those of the reference strains. Mosquitocidal activities of crystal proteins produced by K-1205-1 and K-1381-1 were significantly high by about 40-50 folds at $LC_50$ when compared to those of subsp. kurstaki and subsp. aizawai. Finally, in southern blot analysis using cry1A-type specific probe, K-1205-1 and K-1381-1 had different bands from subsp. kurstaki and subsp. aizawai, respectively. In conclusion, our results suggest that K-1205-1 and K-1381-1 appear to be new moquitocidal B. thuringiensis strains isolated from Korean soil.

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머스크멜론의 과실성숙기 토양수분이 수량과 품질에 미치는 영향 (The Effect of Soil Water Content during at Fruit Ripening Stage on Yield and Quality in Musk melon)

  • 권준국;이재한;엄영철;김회태;최영하;박동금
    • 생물환경조절학회지
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    • 제7권4호
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    • pp.330-335
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    • 1998
  • 네트 멜론 재배에 있어서 과실비대성숙기의 토양수분관리방법이 과실의 수량과 품질에 미치는 영향을 구명하고자 원더풀하계1호 품종을 플라스틱하우스 내에서 착과후 20일부터 관수개시점을 -10, -20, -30, -50, -100kPa로 각각 달리하여 검토한 결과, 경엽의 생체중은 관수량이 가장 많았던 -10kPa에서 가장 무거웠고 -100kPa에서 가벼웠으나 그 차이는 작았으며, 건물율은 100kPa구가 가장 높고 -10kPa에서 낮았다 상품수량은 -50kPa, -100kPa, -30kPa, -20kPa구 사이에는 차이가 없었으나 토양수분이 많았던 -10kPa구에서 가장 적었다. 과실의 크기는 처리간 차이가 없었으며, 당도는 -50kPa구가 15.1 $^{。}$ Brix로 높았던데 비해 -10kPa구가 14.4 $^{。}$ Brix로 가장 낮았다. 이상의 결과로 보아 성숙기에는 -50kPa 도달시 10mm 내외로 관수하는 것이 적당하다고 판단되었다.

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Precurved Endosonic K-file에 의한 근관확대율 및 만곡도 변화에 대한 연구 (COMPARATIVE STUDY ON PERCENTAGE OF CANAL ENLARGEMENT AND CANAL ANGULATION CHANGE BY PRECURVED ENDOSONIC K-FILE)

  • 허혜경;오원만;양규호
    • Restorative Dentistry and Endodontics
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    • 제20권1호
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    • pp.316-327
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    • 1995
  • The purpose of this study was to evaluate canal shaping ability and canal angulation change of K-file, straight endosonic K-file and pre curved endosonic K-file. Twenty staight canals and thirty curved canals were selected and divided into five groups according to canals curvature and canal instrumentation method. VI group was prepared by straight endosonic K-file and H1 group by K-file in straigt canals. V2 group was instrumented by straight endosonic K-file, V3 group by pre curved endosonic K-file and H2 group by precurved K-file in curved canals. Radiographs of canals were obtained before and after canal shaping. And postoperative radiographs were compared with preoperative radiographs using superimposition method. The results obtained were as follows ; l. In straight canals, K-file group demonstrated lager percentage of canal enlargement than endosonic K-file group on facial view, but reverse results exhibit on mesial view. 2. In curved canals, precurved K-file group showed largest percentage of canal enlargement, followed by precurved endosonic K-fine group and straight endosonic K-file group was smallest. 3. Percantage of canal enlargement at convex side was greater than at concave side in apical portion of each group. Especially in straight endosonic K-file group, percantage of canal enlargement at convex side and concave side showed sharply difference. 4. In angulation change, the straight endosonic K-file group exhibited the greatest its change, followed by precurved endosonic K-file group and precurved K-file group was the least. Above results suggest that K-file is more effective endodontic instrument than endosonic K-file, and that precurved file is effective for canal shaping in curved canal.

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히스톤 H3K27 변형과 유전자 전사 (Histone H3K27 Modifications and Gene Transcription)

  • 김애리
    • 생명과학회지
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    • 제21권4호
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    • pp.616-620
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    • 2011
  • 진핵세포의 크로마틴에서 히스톤 단백질 H3와 H4의 라이신 잔기는 공유결합에 의해 변형된다. 히스톤 H3에서 27번 라이신은 아세틸화되거나(H3K27ac) 세 가지 단계로 메틸화가 될 수 있으며(H3K27me1, H3K27me2, H3K27me3), 이러한 H3K27의 변형들은 각각 독특한 형태로 유전자 전사 및 크로마틴 구조와 관련된다. 일반적으로 H3K27ac과 H3K27me1은 좌위조절부위나 활발히 전사되는 유전자처럼 활성 크로마틴에서 나타나고, 이에 반해 전사가 일어나지 않은 유전자는 높은 수준의 H3K27me2과 H3K27me3이 관찰된다. 이러한 변형들은 각각 다른 종류의 변형효소에 의해 촉매된다. 최근 연구들은 유전자 전사 및 크로마틴 구조 형성에서 H3K27의 네 가지 변형들 사이에 상관 관계가 있음을 제시하고 있다.