• 한국수산과학회지
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• 제55권3호
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• pp.379-385
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• 2022

This study was conducted to evaluate the dietary supplementation of citrus byproduct (CBP) on the growth performance, feed utilization, and innate immune responses of juvenile olive flounder Paralichthys olivaceus under low water temperatures (11-15℃). Dietary L-ascorbyl-2-polyphosphate was replaced with graded CBP levels at 0 (Con), 25 (CBP25), 50 (CBP50), 75 (CBP75), and 100% (CBP100). Triplicate groups of juvenile olive flounder were handfed with one of the diets twice a day for 42 days. The growth performance and feed utilization of fish fed with diet containing levels of CBP75 or CBP100 increased significantly compared to those of fish with fed Con. Dietary CBP supplementation increased the protein efficiency ratio in fish. There was no significant differences in innate immune responses between groups, even though CBP supplementation tended to increase. These findings indicate that CBP could be used as a vitamin C source and improve the growth performance of juvenile olive flounder under low water temperatures.

• 한국양식학회지
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• 제10권3호
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• pp.289-300
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• 1997

실험실에서 사육시킨 Neomysis intermedia 의 섭이, 성장, 탈피, 생식 및 대사에 관한 자료로부터 생활사에 따른 에너지수지를 분석하였다. 1. 치하 및 성체의 성장은 암수 모두 초기성장이 빠르고 성장해 감에 따라 성장률이 현저히 저하되어 암컷의 경우 최대체장이 8.55 mm에 도달하여 6.87 cal 그리고 수컷의 최대체장은 7.53 mm로서 5.67 cal 에너지를 체성장에 사용하였다. 2. 성장에 수반되어 탈피체로 손실된 에너지는 암컷이 0.46 cal, 수컷은 0.38 cal의 에너지를 탈피에 사용하였다. 3. N. intermedia 암컷의 생식에 사용된 에너지는 6.75 cal 이었다. 4. 호흡에 사용된 에너지는 암컷이 48.48 cal, 수컷이 36.45 cal 이었다. 5. 치하 및 성체의 암컷은 84.15 cal의 에너지를 섭이를 통해 섭취하였으며, 수컷은 67.09 cal를 섭취하였다. 6. 요로 배설된 에너지는 암컷이 10.36 cal, 수컷은 6.46 cal 이었으며 분배출은 암컷이 11.23 cal, 수컷이 12.08 cal의 에너지를 배출하였다. 7. 섭취한 에너지에 대한 동화효율은 암컷이 86.65%, 수컷이 81.99%이었다. 8. 성장으로 전환된 에너지의 섭이에너지에 대한 백분율인 총성장효율(K 하(1))은 암컷이 8.71%, 수컷이 9.02%이었다. 9. 성장으로 전환된 에너지의 동화에너지에 대한 백분율인 순성장효율(K 하(2))은 암컷이 10.05%, 수컷은 12.36%이었다. 10. 동화에너지에 대한 대사에너지의 백분율은 암컷이 66.48%, 수컷이 66.26% 였다.

• 환경생물
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• 제31권4호
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• pp.353-361
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• 2013

해양에서 이산화탄소 증가에 따른 해양산성화가 넙치치어의 성장에 미치는 영향을 조사하기 위하여 현장 메조코즘 실험을 실시하였다. 동해 남부에 위치한 기장군 장안읍의 사질저질에서 실험군(이산화탄소 주입)과 대조군의 실험장비를 설치하여 43일 동안 총 447개체 (실험군 223개체, 대조군 224개체)를 대상으로 실시하였다. 실험군의 pH는 평균 $7.63{\pm}0.13$, 이산화탄소 ($fCO_2$)농도는 평균 $1660{\pm}540$ ${\mu}atm$로 2100년도에 예상되는 수준으로(IPCC 2007) 유지하였으며, 대조구는 현재 기장군 장안읍 해역의 해양환경으로 유지하였다. 실험기간동안 사망률은 실험군과 대조군 사이에서 큰 차이를 나타내지 않았고 시간이 지남에 따라 감소하는 경향을 나타내었다. 넙치치어의 체장과 체중은 대조군에서 더 큰 증가양상을 나타내어, 그 결과 대조군에서 더 큰 성장률(specific growth rate)을 보였다. 결론적으로 본 연구는 해양에서 이산화탄소 농도의 증가는 넙치 치어의 초기 성장에 영향을 줄 수 있는 가능성을 제시하였다.

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.127-132
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• 2010

A study was conducted to investigate the effects of dietary lipid sources on the growth and fatty acid composition of juvenile sea cucumber. For 12 weeks, three replicate groups of sea cucumber (average weight 1.4 g) were fed one of three diets, containing squid liver oil (SLO), soybean oil (SO), or linseed oil (LO) as a dietary lipid source, or a control diet (CON) without added lipids. Sea cucumber survival was not significantly different among dietary treatments (P>0.05). The highest weight gain was observed in sea cucumber fed the SLO diet, whereas the weight gain of sea cucumber fed the SO diet was the lowest among dietary treatments (P<0.05). No significant differences were found in the moisture, crude protein, crude lipid, and ash contents of whole sea cucumber body among dietary treatments (P>0.05). Concentrations of 20:5n-3 and 22:6n-3 were significantly higher in sea cucumber fed the SLO diet than in those fed on the other diets. The highest 18:2n-6 and 18:3n-3 contents were observed in sea cucumber fed the SO and LO diets, respectively. The results of this study suggest that squid liver oil could be used as a good lipid source in formulated diets for juvenile sea cucumber.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.

• Fisheries and Aquatic Sciences
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• 제26권1호
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• pp.58-68
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• 2023

This study was conducted to determine dietary threonine (Thr) requirement for juvenile olive flounder (Paralichthys olivaceus). A total of 450 juvenile fish (23.2 ± 0.4 g) were randomly distributed to 18 tanks (215 L) with 25 fish per tank. Experimental diets included with graded levels of Thr at 0.0%, 0.4%, 0.8%, 1.2%, 1.6%, and 2.0% were assigned for triplicate groups of fish and fed two times daily to apparent satiation for 12 weeks. Weight gain, specific growth rate, feed intake, feed utilization and survival were significantly (p < 0.05) increased in fish fed with dietary Thr levels over 0.8%, and no significant differences were observed between 0.8% to 2.3% levels. Non-specific immune parameters of serum lysozyme, myeloperoxidase activity, antiprotease activity, and total immunoglobulin were significantly increased by dietary Thr over 0.8%. Based on the broken-line regression analysis, the Thr requirement for the optimum growth and immune response in olive flounder is likely to be 1.03% in the diet.

• 한국어류학회지
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• 제32권2호
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• pp.70-77
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• 2020

최초 체중 3.09±0.02 g 조피볼락 치어에서 먹이원으로 냉동크릴 (FWK) 그리고 크릴분말 (KM) 첨가량 (0~40%)에 따라 6종 실험사료로 7주간 사육한 결과 최종 체중, 증체율, 사료효율 및 전어체의 일반성분 중 회분에서는 유의적으로 차이가 나타났지만, 생존율과 전어체의 일반성분 중 수분, 조단백질 및 지질에서는 유의적인 차이 없이 유사하였다. 사육실험 후 크릴 첨가 먹이 종류에 따른 최종 체중은 7.4~12.0 g, 증체율은 141~286%로 먹이 종류에 따라서 유의적으로 성장 차이를 나타내었다. 사료효율은 36~72%로 성장처럼 유사한 경향을 보였다. 크릴을 첨가하지 않은 대조구에서는 12.0 g으로 FWK10구에서 9.3 g과 유의적으로 유사한 최종 체중을 나타내어 다른 시험구보다는 높은 최종 체중을 나타내었다. 즉, FWK 20% 이상 및 크릴분말 0%, 10%, 20%구에서는 7.4~7.8 g으로 유의적으로 낮게 나타났다. 냉동크릴구에서 냉동크릴 무첨가구와 유사하거나 냉동크릴 및 크릴분말 함유량에 따라 유의적으로 낮은 전어체 회분 함량을 나타내었다. 또한 FWK20KM20구에서 어분으로 단백질 함량을 높여주어도 성장, 사료효율 및 전어체의 일반성분에서 차이가 나지 않았다. 이상의 결과로 조피볼락 치어 사료에 냉동크릴 및 크릴분말 10~40% 첨가 시 성장, 사료효율 및 생존율 향상에 효과가 없었다. 그러나 냉동크릴만 10% 첨가 시에는 어분만을 첨가한 사료와 유사한 양호한 성장, 사료효율 및 생존율을 나타내었다.

• 한국수산과학회지
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• 제46권4호
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• pp.377-383
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• 2013

We ran a feeding trial to determine optimal dietary protein and lipid levels for growth of juvenile long snout bullhead Leiocassis longirostris Gunther. Eight experimental diets (P20L7, P20L14, P30L7, P30L14, P40L7, P40L14, P50L7 and P50L14) were formulated to contain 20%, 30%, 40% or 50% protein combined with either 7% or 14% lipid. Three replicate groups of fish (mean mass: 3.9 g/fish) were fed one of the experimental diets ad libitum for 8 weeks. Survival of fish fed the P20L14 diet was lower than that of fish fed the P40L14, P50L7 and P50L14 diets. Growth of fish fed diets containing 7% lipid increased with increasing protein level (up to 50% protein); growth of fish fed diets containing 14% lipid increased with increasing protein level (up to 30% protein). The feed efficiency of fish fed a diet with 50% protein and 7% lipid was higher than that of other groups. Whole body moisture and lipid contents were affected by dietary lipid level but not by dietary protein level. The crude lipid contents of fish fed 14% lipid diets were higher than those fed 7% lipid diets across all protein levels (other than the 50% level). Thus, under our experimental conditions, an increase in dietary protein level improved growth and feed efficiency of fish; a diet containing 50% protein with 7% lipid was optimal for growth and effective feed utilization in juvenile long snout bullhead.

• Fisheries and Aquatic Sciences
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• 제5권2호
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• pp.122-126
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• 2002

A feeding trial was conducted to determine compensatory growth of juvenile olive flounder in the spring. Five treatments of fish with 3 replicates were prepared: the control group fish (C) fed twice daily for 8 weeks, the Sl, S2, S3 and S4 fish fed for 7, 6, 5 and 4 weeks after 1, 2, 3 and 4 weeks of starvation, respectively. Survival of olive flounder was not significantly different among treatments. Weight gain of flounder in S2 was significantly (P<0.05) higher than that of fish in S3 or S4, but not significantly different from that of fish in C or Sl. The poorest weight gain was observed in fish of S4 treatment. Specific growth rate (SGR) for flounder in S2 was significantly (P<0.05) higher than that for fish in C or S4, but not significantly different from that of fish in Sl or S3. Feed intake (g/fish) was proportional to duration of days of feeding except for flounder in S2, but not significantly different among C, Sl or S2. Feed efficiency ratio (FER) and protein efficiency ratio (PER) for flounder in S2 were significantly (P<0.05) higher than for fish in C, but not significantly different from those for fish in Sl, S3 or S4. Hepatosomatic index (HSI) and condition factor (CF) for flounder in Sl, S3 and S4 were not significantly different from those for fish in C, but significantly (P<0.05) lower than S2 except for CF in Sl at the end of the experiment. Proximate composition of the whole body of flounder was not significantly different among treatments. In considering results of the experiment, juvenile olive flounder achieved compensatory growth when properly fed after starved up to 2 weeks in the spring. Compensatory growth of fish was supported by improvement in SGR, FER and PER in fish starved.

• 한국양식학회지
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• 제19권2호
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• pp.95-98
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• 2006

This study was performed to determine possibility of compensatory growth of juvenile olive flounder fed a commercial feed during the summer season. Five treatments of fish with triplicates were prepared: C, S1, S2, S3 and S4. Fish in the control group (C) was hand-fed with the commercial feed to apparent satiation twice daily for 6 days a week during 6 weeks. Fish in S1, S2, S3, and S4 experienced 1, 2, 3, and 4 weeks of starvation before fed to satiation twice daily for 5, 4, 3, and 2 weeks, respectively. The feeding trial lasted far 6 weeks. Survival of flounder in C, S1 and S2 was significantly (P<0.05) higher than that offish in S4. Weight gain and specific growth rate (SGR) of flounder in C and S1 were significantly (P<0.05) higher than those of fish in S2, S3 or S4. And weight gain and SGR of flounder in S2 and S3 were significantly (P<0.05) higher than those of fish in S4. Feed consumption of flounder tended to increase with weeks of feeding. Feed efficiency ratio and protein efficiency ratio for flounder in C, S1, S2 and S3 were significantly (P<0.05) higher than those for fish in S4. Moisture content of the whole fish in C was lowest, but highest for fish in S4, respectively. Crude protein content of the whole fish in C was highest, but lowest far fish in S4, respectively. Crude lipid content of the whole fish in C, S1 and S2 was significantly (P<0.05) higher than that of fish in S4. In conclusion, full compensatory growth was obtained in juvenile olive flounder fed for 5 weeks after 1-week feed deprivation during the summer season. Compensatory growth of fish was well supported by improvement in feed efficiency ratio and protein efficiency ratio.