• Archives of Pharmacal Research
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• 제27권1호
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• pp.35-39
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• 2004

The method for the chiral analysis of (-)-yatein was developed and the distribution of this component in the plants of three genera like Juniperus, Thuja and Chamaecyparis belonging to Cupressaceae family was examined. The chiral analysis of (-)-yatein from the plants was carried out by high performance liquid chromatography on (R,R)-Whelk-O1 column using 81 v/v% methanol as mobile phase. The yatein content in the leaves of Juniperus was the highest in compare with that of the other two genera, providing the possibility of the chemical discrimination of the plants in Juniperus from the other plants in the Cupressaceae family. In general, the yatein content in the leaves was much higher than that in the twigs. This method could be applied for the quality control of (-)-yatein in the plants belonging to Cupressaceae family.

• Natural Product Sciences
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• 제4권1호
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• pp.9-14
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• 1998

Jaceidin, Jaceidin-7-O-methylether and quercetin were isolated from-Juniperus phoenicea L. alcoholic extract, however, Sequoiaflavone was isolated from Cupressus semperiverns L. In addition, the alcoholic extracts of both plants were found to contain also kaempferol-3-O-rhamnoside, quercetrin, myricitrin, cupressuflavone. The chemical identities of the isolated compounds were established using UV, IR, $^1H-and\;^{13}C-NMR$ spectroscopy.

• 조경수
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• 통권121호
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• pp.18-19
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• 2011

• Journal of Ecology and Environment
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• 제42권3호
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• pp.111-119
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• 2018

Background: Juniperus chinensis L. populations are distributed locally on several areas including coastal cliffs which are difficult to access in the central eastern Korea. Wild populations inhabit relatively barren environments such as rocky areas and cliffs, which are very sensitive to even minor environmental disturbances including artificial interventions and natural disturbances, and thus demonstrate great fluctuations in the population size and density. This study aims to analyze the genetic diversity, differentiation, and genetic structure of each population in order to provide useful data required to establish a substantial conservation strategy of J. chinensis. Results: The genetic diversity of J. chinensis at the population level (P = 78.7%, h = 0.282, S.I. = 0.420) was somewhat higher compared with those measured in the same genus, Juniperus. The genetic differentiation degree among nine populations established naturally in central eastern Korea was 11.50% and that among sub-populations within the same area was 5.52%. On the other hand, genetic variation of individuals within the populations was 82.93%. But frequency of the main allele was different among loci. In particular, fixation of allele frequency and occurrence of rare allele in the highly isolated population suggest a likelihood that genetic drift would occur in populations of this plant. As the result of analysis on the genetic structure of nine populations, nearby populations and isolated populations tended to form separate clusters from each other as the hypothetical number of clusters (K) increase. Conclusions: This result implies that if the population size of J. chinensis is reduced due to environmental change and artificial and/or natural disturbances in the future, it could affect negatively on the genetic diversity of the plant species. In order to maintain and conserve genetic diversity of J. chinensis, ecological network, which can help genetic exchange among the local populations, should be prepared, and conservation strategies in situ as well as ex situ are also required with continuous monitoring.

• Applied Biological Chemistry
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• 제41권5호
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• pp.395-398
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• 1998

생약에서 충치균에 대한 항균성 물질을 개발하기 위하여 수종의 야생생약을 수집하여 항균활성을 측정해 보았다. 그 중에서 충치균에 강한 항균활성을 나타낸 두송실(Juniperus rigida S. et Z.)을 methanol로서 추출하고 극성이 다른 수종의 용매로서 분획하여, 충치균에 대한 항균활성과 유기산의 생성억제효과를 측정해 본 결과, n-hexane, chloroform 분획에서 활성이 나타났다. 충치균에 의하여 생성된 유기산은 주로 lactic acid와 citric acid로 전체의 $80{\sim}90%$를 차지하였는데 두송실의 methanol추출물과 n-hexane분획물을 1 mg/ml 처리한 시험군에서 각각 89.3, 90.8%의 유기산 생성 억제효과를 나타내었다.

• Applied Microscopy
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• 제42권3호
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• pp.124-128
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• 2012

Leaf surface structures were investigated in the Chinese juniper Juniperus chinensis by scanning electron microscopy. Adult scale leaves were collected from the tree, air-dried at room temperature, and sputter-coated with gold without further specimen preparation. Approximately fi ve stomata were locally distributed and arranged in clusters on the leaf surface. Stomata were ovoid and ca. 40 ${\mu}m$ long. The epicuticular wax structures of J. chinensis leaves were tubules and platelets. Numerous tubules were evident on the leaf regions where stomata were found. The tubules were cylindrical, straight, and ca. 1 ${\mu}m$ in length. They almost clothed the stomatal guard cells, and occluded the slit-shaped stomatal apertures. Moreover, the wax ridges were flat crystalloids that were connected to the surface by their narrow side. They did not have distinct edges, and their width/height ratio varied. In particular, the wax ridges could be discerned on the leaf regions where stomata were not present nearby. Since the wax ridges did not have distinct edges on their margin, they were identified as platelets. Instances were noted where platelets were oriented either parallel to each other or perpendicular to the cuticle surface. These results can be used in biomimetics to design the hierarchical structures for mimicking the plant innate properties such as hydrophobicity and self-cleaning effects of the leaf surface.

• 한국조경학회지
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• 제18권2호
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• pp.57-65
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• 1990

To select suitable siecies for barrier trees in the middle tmperature forest zone of Korea, sprout ratio, verdi cal density ratio and impenetrability were analyzed for 41 species including 9 evergreen conifer trees which had been transplanted 18 years ago and maintained as a hedge at Pocheon - Gun, Kyonggi -Do, Korea. 1. The species with high sprout ratio of hedges were Rosa multiflora, Lonicera maackii, Chamaecyparis pisifera, Thuja occidentalis in order at the upper portion, Thuja occidentalis, Rosa multiflora, Chamaecyparis pisifera, in order oa the middle portion, and Thuja occidentals, Chamaecyparis pisigera, Rosa multiflora, Cercdiphyllum japonicum, Cornus kousa, Ulmus pumila, Robinia pseudoacacia in order at the lower portion of hedges. 2. the species with high vertical density ratio were Thuja occidentalis, Chamaecyparis pisifera, Weigela subsessilis, Acer ginnala in order. 3.The species with hight impnetrability were Thuja occidentalis, Chamaecyparis pisifera, Forsythia Koreana, Juniperus rigida, Juniperus chinensis, Pinus strobus in order. 4. the values of correlation coefficents between the sprout ratio and impenetrability are given in table 3 and positive significant correlations can be observed in conifer trees.5. The species with high suitability for tree barrier were Thuja occidontalis, Chamaecyparis pisifera, Acer ginnala, Juniperus chinensis, Rosa multiflora, Counus kousa, Cercidiphyllum japonocum, Weigela subsessilis, Cornus officinalis, Philadelphus schrenckii in order.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.729-732
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• 2000

양파의 저온저장중 부패방지를 위한 천연항균제 개발의 일환으로 저장양파로부터 부패세균을 분리한 후 이 균에 대한 식물추출물의 항균활성을 조사하였다. 양파의 저온저장중 과육으로부터 분리한 곰팡이를 MEA와 PDA에서 배양하면서 분생자병과 분생자의 형태적 특성을 조사한 결과 플라스크형의 phialide, 분생자병의 branching type이 simple type이며, conidial head가 columnar shape 가지고 있는 것으로 확인되어 Penicillium sp. ON-211으로 동정하였다. 또한 자생 식물은 채집하여 각종 유기용매로 추출한 후 항균활성을 조사하였다. 정향나무, 탱자나무의 잎은 chloroform 분액에서 높은 활성을 나타내었고, 측백나무 열매는 물분획에서 높은 활성을 나타내었으며, 연필향나무의 잎은 ethyl acetate 분획에서 비교적 높은 항균 활성이 나타났다.

• Natural Product Sciences
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• 제24권2호
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• pp.125-131
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• 2018

Berries and branches essential oil of Juniperus phoenicea were obtained by electromagnetic induction heating assisted extraction and by hydrodistillation with a yield varied from ($1.2{\pm}0.3$ to $2.4{\pm}0.7%$) and from ($0.6{\pm}0.1%$ to $1.1{\pm}0.1%$), respectively. forty eight compounds were identified representing (97.2 - 99.7%) of the oil. ${\alpha}$-Pinene (40.3 - 67.8%) and ${\delta}$-3-carene (13.5 - 26.8%) were the main compounds in berries and branches essential oils. Antioxidant activity was evaluated by three means: inhibition of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical, reducing power and ${\beta}$-Carotene/linoleic acid bleaching. The antioxidant activity of essential oils showed $IC_{50}$ ranging from $67.6{\pm}1.02{\mu}g/mL$ to $131.5{\pm}0.8{\mu}g/mL$ for berries and from $98{\pm}1.25{\mu}g/mL$ to $166.8{\pm}0.29{\mu}g/mL$ for the branches. Berries oil show more potent antioxidant activity compared to branches. This result is supported by the three methods investigated in this work.

• 생약학회지
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• 제43권3호
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• pp.239-242
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• 2012

This study was carried out to investigate the anti-malarial activity of Juniperus chinensis by in vitro and in vivo system using Plasmodium falciparum chloroquine-sensitive(3D7) and P. falciparum chloroquine-resistant(S20) strains. According to cytotoxicty test on NIH 3T3 cell, the ethanol extract(EtOH), ethylacetate(EtOAc) fraction and aqueous fraction possessed significant anti-malarial activity against both 3D7 and S20 strains at non-toxic concentrations(<100 /). In vitro assay, EtOAc fraction showed notable activity against 3D7 and S20 strains of P. falciparum with $IC_{50}$ values of $37{\pm}2{\mu}g/ml$ and $36{\pm}6{\mu}g/ml$. In animal test using P. falciparum infected human erythrocytes, the treatment of EtOAc fraction significantly inhibited parasitaemia in mice in a dose-dependent manner that is parasitaemia of 42%, 34% and 31% in doses of 10 mg/kg, 20 mg/kg and 40 mg/kg, respectively. The study provides data to support the medicinal importance of the J. chinensis.