• Title/Summary/Keyword: John School in Korea

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Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins

  • Lim, Hyoun-Sub;Lee, Mi Yeon;Moon, Jae Sun;Moon, Jung-Kyung;Yu, Yong-Man;Cho, In Sook;Bae, Hanhong;DeBoer, Matt;Ju, Hojong;Hammond, John;Jackson, Andrew O.
    • The Plant Pathology Journal
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    • v.29 no.1
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    • pp.17-30
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    • 2013
  • Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

Hierarchical Regimentation of Korean Language Uses (반말의 비인문성 -철학자가 본 한국의 언어연구-)

  • 정대현
    • Lingua Humanitatis
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    • v.5
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    • pp.75-92
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    • 2003
  • One of the distinctive features of Korean language is that it has a fine hierarchical regimentation of language uses, perhaps finer than English, Chinese or Japanese. If English language uses have a hierarchical structure they are to be thick. You may respond to any of your male colleagues often by saying either "Yes, Sir" or "Yes, John". But Korean speakers attend to the One grades of differences of social positions of a speaker and a hearer and they show the respect of the difference by adding or dropping relevant suffixes of verbs which Korean language has developed. For example, one yew difference would affect how you choose a suffix of verbs you use to speak to your hearer and two year difference often leads to the adoption of still another fitting suffix of the same verb. One year criterion works not only in 3my barracks, school dormitories but also in government offices. business sectors. Korean speaking people have been taught to use this finely regimented hierarchical language. I try, in this paper, to develop the idea that hierarchical regimentation of Korean language uses is not humane. 1 of for the main argument for the thesis as what follows: How could one justify the hierarchical regimentation of a language like Korean\ulcorner Only if there is an essential structure in which the fine grades of differences of social positions of all the people are distinct; The essentialism here involved is not plausible. And I may add that language is to be used fur the purposes of communication, rationalization and expression. If true, language use is a genuine art of liberation or humanization. Any overt hierarchical language tends to damage those purposes and more to enforce those oppressive elements already existing in the community. Then, a hierarchical language is to defeat its own purpose.

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Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

  • Choi, Go-Woon;Oh, June-Pyo;Cho, In-Sook;Ju, Hye-Kyoung;Hu, Wen-Xing;Kim, Boram;Seo, Eun-Young;Park, Jong-Seok;Domier, Leslie L;Hammond, John;Song, Kihak;Lim, Hyoun-Sub
    • The Plant Pathology Journal
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    • v.35 no.5
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    • pp.538-542
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    • 2019
  • In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

Knowledge of the negative effect of Cigarette Smoking among adult in sokoto metropolis

  • John, Ikpeama Osita;Mariam, Onuzulike Nonye;Adimabua, Okafor Patrick;Anthonia, Ikpeama Chizoba;Joy, Ikpeama Chinwe;Osazuwa, Igbineweka Osa;Andrew, Ikpeama Emeka;Jacob, Ofuenyi;Paulastella, Nwosu Nchedochukwu;Nnanna, Ibeh Isaiah;Mokwe, Gerald Chukwudi
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.2
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    • pp.13-25
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    • 2019
  • This study was aimed at ascertaining the level of knowledge of the health effects of cigarette smoking on health and wellbeing among adults. A cross-sectional form of descriptive survey research design was used for this study. Two hundred and seventy copies of the questionnaire collected were analyzed using descriptive statistic of frequency count, normative percentage and grand mean; as well as inferential statistics of chi-square (${\chi}^2$). The level of significant was fixed at 0.05. Appropriate degrees of freedom were worked out. The influence of gender on the knowledge of the negative effect of cigarette smoking among adults in sokoto. The results shows that 48% agreed on the negative effects of showing among male followed by 29.3% among female, those who undecided 8.8% male and 4.4% females while those who disagree are 6.2% for male and 2.5% for female. The research concluded that the knowledge of negative effect of cigarette smoking among adults in sokoto metropolis was very good across the different variable (marital status, gender, and year in higher institution) with single, male and first year in higher institution showing significantly good knowledge of the negative effect of cigarette smoking.

Adding AGC Case Studies to the Educator's Tool Chest

  • Schaufelberger, John;Rybkowski, Zofia K.;Clevenger, Caroline
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.1226-1236
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    • 2022
  • Because students majoring in construction-related fields must develop a broad repository of knowledge and skills, effective transferal of these is the primary focus of most academic programs. While inculcation of this body of knowledge is certainly critical, actual construction projects are complicated ventures that involve levels of risk and uncertainty, such as resistant neighboring communities, unforeseen weather conditions, escalating material costs, labor shortages and strikes, accidents on jobsites, challenges with emerging forms of technology, etc. Learning how to develop a level of discernment about potential ways to handle such uncertainty often takes years of costly trial-and-error in the proverbial "school of hard knocks." There is therefore a need to proactively expedite the development of a sharpened intuition when making decisions. The AGC Education and Research Foundation case study committee was formed to address this need. Since its inception in 2011, 14 freely downloadable case studies have thus far been jointly developed by an academics and industry practitioners to help educators elicit varied responses from students about potential ways to respond when facing an actual project dilemma. AGC case studies are typically designed to focus on a particular concern and topics have thus far included: ethics, site logistics planning, financial management, prefabrication and modularization, safety, lean practices, preconstruction planning, subcontractor management, collaborative teamwork, sustainable construction, mobile technology, and building information modeling (BIM). This session will include an overview of the history and intent of the AGC case study program, as well as lively interactive demonstrations and discussions on how case studies can be used both by educators within a typical academic setting, as well as by industry practitioners seeking a novel tool for their in-house training programs.

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Preparation and Characterization of PLGA Scaffold Impregnated Keratin for Tissue Engineering Application (케라틴이 함유된 조직공학적 PLGA 지지체의 제조 및 특성 분석)

  • Oh, A-Young;Kim, Soon-Hee;Lee, Sang-Jin;Yoo, James J.;Dyke, Mark van;Rhee, John M.;Khang, Gil-Son
    • Polymer(Korea)
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    • v.32 no.5
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    • pp.403-408
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    • 2008
  • Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.

International Comparison of Humane Entrepreneurship: Status, Typology and Policy Implications (사람중심 기업가정신의 국제비교 연구)

  • Kim, Ki-Chan;Bae, Zong-Tae;Enriquez, John Laurence;Song, Chang Seok
    • The Journal of Small Business Innovation
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    • v.20 no.4
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    • pp.23-32
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    • 2018
  • Humane entrepreneurship is defined as "the pursuit of entrepreneurial growth and humane development for opportunity realization and sustainable organization." In this study, we evaluate and compare 19 countries by the degree to which 'humane entrepreneurship' is realized or emphasized. This study compares two factors of humane entrepreneurship, enterprise cycle and humane cycle, between different countries and examines a balance between the two. To make country-by-country comparison along humane entrepreneurship, we adopted Sethia and von Glinow's (1985) organizational culture model, which originated from Blake and Mouton's (1964) managerial grid model. Here, enterprise cycle and humane cycle of humane entrepreneurship correspond to concern for performance and concern for people. We suggest that keeping balance between humane cycle and enterprise cycle is important, because humane entrepreneurship is an internal condition for firm growth.

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A Study on the Professional Consciousness of Crew based on the Characteristics of Demography and On-board Working (선원 개인적 특성과 승선근무 특성에 따른 선원직업의식에 관한 연구)

  • Shin, Yong-Joo;Im, Seong-Ju;Suha, Nadhilla;Shin, Yong-John
    • Journal of Navigation and Port Research
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    • v.42 no.4
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    • pp.299-310
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    • 2018
  • This study examined the significance of the crew's professional consciousness and sought to clarify the differences in the professional consciousness of crew by considering biographical characteristics of the crew and onboard working situations, which were expected to influence the professional consciousness of crew. The empirical analysis showed the crew's individual characteristics such as age, marital status, position in ship organization and nationality significantly influenced professional consciousness. The comparison in the differences of professional consciousness level among crew's nationalities showed that the professional consciousness of Filipino crew was the highest, and in the descending order of Indonesia, Myanmar, Chinese, and South Korea. The analysis results of other variables of on-board working characteristics showed that as crew's satisfaction of welfare increased, crew's professional consciousness increased, whereas the feeling of isolation from home and society had a statistically significant negative (-) impact on crew's professional consciousness. Furthermore, the analysis of multinational crew manning characteristics stated that cross-cultural acceptance did not have a statistically significant impact on the crew's professional consciousness, while cross-cultural adaptability had a statistically significant impact. This study emphasize the importance of the crew's professional consciousness and suggest how to enhance it, which allows crew to continue their career with the charm and pride of their profession.

HIV/AIDS Management: Dolutegravir Based Antiretroviral Drug Therapy

  • John, Ikpeama Osita;Emmanuel, Okoh Emeka;Anthonia, Ikpeama Chizoba;Joy, Ikpeama Chinwe;Adimabua, Okafor Patrick;Osazuwa, Igbineweka Osa;Andrew, Ikpeama Emeka;Mariam, Onuzulike Nonye;Gami, Hilary Tumba
    • The Korean Journal of Food & Health Convergence
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    • v.6 no.4
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    • pp.17-19
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    • 2020
  • HIV/AIDS disease still remain a global pandemic and it's management has undergone series of treatment changes and improvement although there is still no permanent cure.Dolutegravir belongs to a group of HIV drugs called integrase inhibitors. Integrase inhibitors block an HIV enzyme called integrase. By blocking integrase, integrase inhibitors prevent HIV from multiplying and can reduce the amount of HIV in the body.Dolutegravir combination based regimen has turned out to be very effective (antiviral) with negligible rare side effects on clients. This drug (Dolutegravir based regimen) combination has successfully increased the appetite for food of all the clients, unlike others and has shown to reduce viral load in the most shortest period ever. It can be deduced that development of resistant mutant virus will be reduced if not eliminated with dolutogravir based regimen.The role of Continuous adherence counseling has shown to improve clients treatment management. It is important to note that the availability of food has direct effect on the economic status or financial weight on the client. Hence the progress that is increase in body mass index (BMI) is a direct impact of the availability of food for the clients.

The Comparative Analysis on the Scrap Transport Costs of Container and Bulk Ship (스크랩(Scrap) 화물의 운송경로별 경제성 평가에 관한 연구)

  • Park, Jae-Kwan;Joung, Eun-Sun;Shin, Yong-John
    • Journal of Navigation and Port Research
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    • v.33 no.8
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    • pp.563-572
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    • 2009
  • The steel industry, as the national representative strategic industry of our country, has played the motive power for the economic growth of Korea in 1970s as the positive support of the government and the management endeavor of the private were harmonized. However, in case of our country, we have mostly relied on import of the raw materials for the steel industry, and as the weight of the imported raw materials is heavy, the steel industry is the industry whose transport burden is big as it is called 'transport industry'. So, the transport rationalization will be the important task of the steel industry. This study has analyzed the economic efficiency per the transport route (container ship vs bulk ship) of the sea transport related to import/export of the steel scrap on the level acquiring the stable supply of the steel scrap. For this, this study firstly researched the status of domestic/foreign steel industry. And analyzed the world crude steel production volume, steel scrap consumption volume and world steel scrap trading structure. Also, in order to compare the transport logistics expenses between two transport devices, namely, container ship and bulk ship, this study calculated the logistics expenses per ton by using the traffic hours and traffic expenses items as the imported/exported scrap freights of 'D' company.