• 한국수정란이식학회지
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• 제30권1호
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• pp.23-31
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• 2015

Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.

• 한국수정란이식학회지
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• 제31권1호
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• pp.61-64
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• 2016

We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock.

• 한국가스학회지
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• 제24권1호
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• pp.49-55
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• 2020

산업용 혹은 발전용 가스터빈에 사용되는 이중 콘형 예혼합 연소기의 다단 연소 특성을 이해하기 위하여 실험적연구를 수행하였다. 이를 위하여 기존에 모두 경사면에 공급되는 연료를 콘부분으로 일부 할애하는 방식으로 다단연소 방식을 구성하였으며 콘에서 분사되는 연료공급은 축방향과 콘 경사면 방향으로 하였다. 다단연소 연소특성을 이해하기 위하여 콘에서의 연료 분사 방향과 연료 분배율 변화에 대한 NOx와 CO의 배출 농도 그리고 벽면 온도분포를 측정하였다. 그 결과 총 연료에 대한 콘으로의 분배율이 3%인 경우 콘에서의 연료 분사방향에 관계없이 노즐내의 예혼합 영역에서 연료가 공기와 균일하게 혼합됨으로서 연소영역의 고온점 감소에 의하여 NOx 배출 농도가 감소된다. 그러나 콘에서 축방향으로 분사되는 연료분배율이 8%로 증가하는 경우 노즐 내부 예혼합 영역으로의 화염 역화로 인하여 NOx의 배출농도가 오히려 증가하게 된다.

• 한국수정란이식학회지
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• 제30권3호
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• pp.249-255
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• 2015

Diabetes mellitus, the most common metabolic disorder, is divided into two types: type 1 and type 2. The essential treatment of type 1 diabetes, caused by immune-mediated destruction of ${\beta}-cells$, is transplantation of the pancreas; however, this treatment is limited by issues such as the lack of donors for islet transplantation and immune rejection. As an alternative approach, stem cell therapy has been used as a new tool. The present study revealed that bone marrowderived mesenchymal stromal cells (BM-MSCs) could be transdifferentiated into pancreatic cells by the insertion of a key gene for embryonic development of the pancreas, the pancreatic and duodenal homeobox factor 1 (PDX1). To avoid immune rejection associated with xenotransplantation and to develop a new cell-based treatment, BM-MSCs from ${\alpha}$-1,3-galactosyltransferase knockout (GalT KO) pigs were used as the source of the cells. Transfection of the EGFP-hPDX1 gene into GalT KO pig-derived BM-MSCs was performed by electroporation. Cells were evaluated for hPDX1 expression by immunofluorescence and RT-PCR. Transdifferentiation into pancreatic cells was confirmed by morphological transformation, immunofluorescence, and endogenous pPDX1 gene expression. At 3~4 weeks after transduction, cell morphology changed from spindle-like shape to round shape, similar to that observed in cuboidal epithelium expressing EGFP. Results of RT-PCR confirmed the expression of both exogenous hPDX1 and endogenous pPDX1. Therefore, GalT KO pig-derived BM-MSCs transdifferentiated into pancreatic cells by transfection of hPDX1. The present results are indicative of the therapeutic potential of PDX1-expressing GalT KO pig-derived BM-MSCs in ${\beta}-cell$ replacement. This potential needs to be explored further by using in vivo studies to confirm these findings.

• 한국해양환경ㆍ에너지학회지
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• 제2권1호
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• pp.40-48
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• 1999

해양공학과 관련된 모형실험을 위하여 많이 사용되고 있는 조파수조(2차원수조, 장수조, 사각수조)에 소파장치는 필수적인 장치이다. 본 논문에서는 새로운 개념의 소파장치의 소파성능을 실험을 통하여 살펴보았다. 새로 고안된 소파장치는 소파성능이 우수하고 가볍기 때문에 설치가 용이하다. 또한 넓은 설치공간이 필요치 않아 수조공간 활용에 있어 효과적이며 조파기로부터 발생된 파장에 큰 영향을 받지 않기 때문에 아주 큰 장파만 피한다면 거의 완벽하게 파를 소멸시킬 수 있는 장점을 가지고 있다. 본 소파장치는 일정한 크기의 구멍이 균일하게 배열된 타공판을 입사파의 진행방향과 수평으로 수면밑에 잠기게 설치한 형태로 파가 수평형 타공판을 통과하면서 타공판의 구멍을 통하여 강한 제트 흐름이 형성되면서 파 에너지가 소멸되는 특징을 가지고 있다. 모형실험 결과 수평형 타공판의 소파성능은 타공판의 잠긴 깊이와 판의 전체면적과 뚫린 부분의 면적의 비로 표현되는 공극율에 밀접한 관련이 있으며, 각각의 값은 최대 소파성능을 발휘하는 최적의 값을 가지고 있음을 규명하였다. 수평형 타공판의 앞쪽을 뒤쪽보다 약간 기울게 설치하는 방법도 특정한 주파수 범위내에서는 수평형에 비하여 우수한 소파성능을 보이며, 이때 타공판의 경사각도도 소파장치 성능을 결정하는데 중요한 변수임을 밝혔다.

• 한국수정란이식학회지
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• 제29권1호
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• pp.7-12
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• 2014

본 연구는 한우 번식 기록이 잘 유지되고 있는 4개 농장의 2007년 1월부터 2010년 10월까지의 번식 자료 수집하여 분석하였다. 수태 당 평균 수정 횟수와 평균 공태일은 A농장 $1.7{\pm}0.1$회와 $77.4{\pm}4.8$일, B농장 $1.5{\pm}0.1$회와 $150.8{\pm}11.2$일, C농장 $1.5{\pm}0.1$회와 $90.4{\pm}4.5$일, D농장 $1.4{\pm}0.1$회와 $71.4{\pm}2.5$일이었다. 호르몬으로 발정을 유도하는 D농장을 제외한 3개 농장 531두의 번식 기록으로 분만 후 첫 수정 시기에 따른 평균 수정 횟수와 평균 공태일을 분석한 결과, 총 5개의 수정 시기에 따른 수정 횟수는 30일 이전 첫 수정이 $2.1{\pm}0.2$회로 31일 이후 첫 수정보다 유의적으로 높았다. 번식 장애우 58두에 2가지 배란 동기화법을 사용하여 수태율을 확인해 본 결과, Ovsynsh 법은 55.2%의 수태율을, CIDR-based TAI 법은 65.5%의 수태율을 나타냈다. 농장의 번식률을 높이기 위해서는 정확한 번식 기록 작성, 발정 관찰, 수정 후 임신 감정, 번식 기관 검진, 번식률을 고려한 첫 수정 시기 수정 등이 필요하다.

• 한국수정란이식학회지
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• 제31권3호
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• pp.255-260
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• 2016

The aim of this study is to evaluate the effects of vitamin or mineral supplements on the conception rates of dairy heifers when replacing the last injection of GnRH with hCG in ovsynch protocol (experiment 1) and also to investigate whether the estrus synchronization treatment in the heifer stage affects the conception rates after $1^{st}$ parturition (experiment 2). In experiment 1, 50 heifers were randomly assigned into 3 groups: 20 heifers each in groups 1 and 2, and 10 in group 3. All three groups were treated with an intramuscular injection of GnRH on day 0 (day 0 = the day of program start), $PGF_{2{\alpha}}$ on day 7 and hCG on day 9, and were inseminated on day 10, 12~16h after hCG injection. In group 1 (vitamin group), the heifers were treated with an intramuscular injection of 5 ml of vitamin-ADE $500^{(R)}$, and group 2 (mineral group) was treated twice with an intramuscular injection of 30 ml of mineral supplement-LAPTOVET$^{(R)}$ on a one-week interval beginning on the day of hormone treatment (day 0 and day 7 respectively). Group 3 (control) was treated only with hormones. Pregnancy diagnosis was performed by ultrasonography through a rectal probe. First service conception rates (FSCR) and average services per conception (ASPC) were recorded for all subjects. Of the total 50 heifers, 6 (2 in group 1, 3 in group 2, and 1 in group 3) heifers were eliminated due to accidents during experiment 1. FSCRs were 58.8% (10/17), 66.7% (12/18) and 44.4% (4/9) in groups 1, 2 and 3, respectively. ASPCs were $1.53{\pm}0.72$, $1.27{\pm}0.59$ and $1.63{\pm}0.74$ in groups 1, 2 and 3, respectively. Although there were no significant difference between the groups, relatively good results (higher FSCR and lower ASPC) were obtained in both group 1 and 2. In experiment 2, 11 primiparous cows from group 2 of experiment 1 in heifer stage which had been treated both with the hormones for estrus synchronizing and mineral supplements (ES group), and 12 primiparous cows treated only with minerals (non-ES group) were compared to examine the effects of estrus synchronization program on conception rates after $1^{st}$ parturition. Following the examination, postpartum ASPCs were $1.55{\pm}0.82$ and $2.17{\pm}1.47$ in ES group and non-ES group, respectively. The postpartum average days open (ADO) were $116{\pm}56$ and $197{\pm}93$ in ES group and non-ES group, respectively. Although there were no significant difference between the two groups, desirable results (lower ASPC and shorter ADO) were found in ES group after $1^{st}$ parturiton. In conclusion, experiment 1 indicates that vitamin or mineral supplement with ovsynch protocol may have some positive effect on FSCR and ASPC of dairy heifers, and in experiment 2, ES program in heifer stage had a positive effect on ASPC and ADO following $1^{st}$ parturition.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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• pp.268-268
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• 2012

Atmospheric pressure microwave induced plasmas are used to excite and ionize chemical species for elemental analysis, for plasma reforming, and for plasma surface treatment. Microwave plasma differs significantly from other plasmas and has several interesting properties. For example, the electron density is higher in microwave plasma than in radio-frequency (RF) or direct current (DC) plasma. Several types of radical species with high density are generated under high electron density, so the reactivity of microwave plasma is expected to be very high [1]. Therefore, useful applications of atmospheric pressure microwave plasmas are expected. The surface characteristics of SUS304 stainless steel are investigated before and after surface modification by microwave plasma under atmospheric pressure conditions. The plasma device was operated by power sources with microwave frequency. We used a device based on a coaxial transmission line resonator (CTLR). The atmospheric pressure plasma jet (APPJ) in the case of microwave frequency (880 MHz) used Ar as plasma gas [2]. Typical microwave Pw was 3-10 W. To determine the optimal processing conditions, the surface treatment experiments were performed using various values of Pw (3-10 W), treatment time (5-120 s), and ratios of mixture gas (hydrogen peroxide). Torch-to-sample distance was fixed at the plasma edge point. Plasma treatment of a stainless steel plate significantly affected the wettability, contact angle (CA), and free energy (mJ/$m^2$) of the SUS304 surface. CA and ${\gamma}$ were analyzed. The optimal surface modification parameters to modify were a power of 10 W, a treatment time of 45 s, and a hydrogen peroxide content of 0.6 wt% [3]. Under these processing conditions, a CA of just $9.8^{\circ}$ was obtained. As CA decreased, wettability increased; i.e. the surface changed from hydrophobic to hydrophilic. From these results, 10 W power and 45 s treatment time are the best values to minimize CA and maximize ${\gamma}$.

• Wind and Structures
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• 제29권5호
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• pp.339-359
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• 2019

Pre-stressed concrete poles are among the supporting systems used to support transmission lines. It is essential to protect transmission line systems from harsh environmental attacks such as downburst wind events. Typically, these poles are designed to resist synoptic wind loading as current codes do not address high wind events in the form of downbursts. In the current study, the behavior of guyed pre-stressed concrete Transmission lines is studied under downburst loads. To the best of the authors' knowledge, this study is the first investigation to assess the behaviour of guyed pre-stressed concrete poles under downburst events. Due to the localized nature of those events, identifying the critical locations and parameters leading to peak forces on the poles is a challenging task. To overcome this challenge, an in-house built numerical model is developed incorporating the following: (1) a three-dimensional downburst wind field previously developed and validated using computational fluid dynamics simulations; (2) a computationally efficient analytical technique previously developed and validated to predict the non-linear behaviour of the conductors including the effects of the pretension force, sagging, insulator's stiffness and the non-uniform distribution of wind loads, and (3) a non-linear finite element model utilized to simulate the structural behaviour of the guyed pre-stressed concrete pole considering material nonlinearity. A parametric study is conducted by varying the downbursts locations relative to the guyed pole while considering three different span values. The results of this parametric study are utilized to identify critical downburst configurations leading to peak straining actions on the pole and the guys. This is followed by comparing the obtained critical load cases to new load cases proposed to ASCE-74 loading committee. A non-linear failure analysis is then conducted for the three considered guyed pre-stressed concrete transmission line systems to determine the downburst jet velocity at which the pole systems fail.

• 천문학회지
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• 제51권6호
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• pp.197-206
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• 2018

We present the analysis of KMT-2016-BLG-0212, a low flux-variation ($I_{flux-var}{\sim}20mag$) microlensing event, which is in a high-cadence (${\Gamma}=4hr^{-1}$) field of the three-telescope Korea Microlensing Telescope Network (KMTNet) survey. The event shows a short anomaly that is incompletely covered due to the brief visibility intervals that characterize the early microlensing season when the anomaly occurred. We show that the data are consistent with two classes of solutions, characterized respectively by low-mass brown-dwarf (q = 0.037) and sub-Neptune (q < $10^{-4}$) companions. Future high-resolution imaging should easily distinguish between these solutions.