• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.3
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• pp.576-583
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• 2005

Tooth formation is a complex developmental process that is mediated through a series of reciprocal epithelial-mesenchymal interactions. Several signal pathways and transcription factors have been implicated in regulating molar crown development, but relatively little is known about the regulation of root development. It was reported that NFI-C knockout mice showed abnormal root formation with normal crown. The aims of this study are to elucidate how the NFI-C regulate the determine of root shape and odontoblasts differentiation. We carried out immunohistochemistry using cytokeratin to investigate the role of Hertwig's epithelial root sheath and DSPP mRNA in-situ hybridization to conform the nature of root dentin during root development in NFI-C knockout mice. Cytokeratin reacted with all the HERS cells and the continuity of cytokeratin positive cells between the HERS cells and enamel epithelium was lost in the cervical region both wild and K/O types. After root dentin deposition cytokeratin positive-HERS cells showed irregularity and loss of polarity in the cervical region in K/O type. DSPP mRNA was strongly expressed in odontoblasts of crown and root dentin in wild type mice, whereas expression of DSPP mRNA was restricted in odontoblast of crown dentin in the K/O type. During root formation in NFI-C knockout mice, HERS normally grow out of the crown but fail to induce odontoblast differentiation in root portion. These results suggest that NFI-C may play important roles in odontoblast differentiation during root dentin formation.

• The Journal of the Petrological Society of Korea
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• v.16 no.2 s.48
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• pp.82-99
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• 2007

The Paleogene dikes intruding into the late Cretaceous granodiorite are pervasively observed in the Irun-myeon, eastern Geoje Island. They are classified into three groups: NW-trending acidic dike swarm and WNW- (A-Group) and $NS{\sim}NNE-trending$ (B-Group) basic dike swarms. Based on their cross-cutting relationships, the earliest is the acidic dike group and fellowed by A- and B-Groups in succession. The acidic dikes seem to have intruded into tension gashes induced by the sinistral strike-slip faulting of the Yangsan fault system during the late $Cretaceous{\sim}early$ Paleogene. In terms of rock-type, orientation, age, and geochemistry, A-Group and B-Group are intimately correlated with the intermediate and basic dike swarms in the Gyeongju-Gampo area, respectively. These results significantly suggest that the corresponding dike swarms are genetically related. Based on the K-Ar and Ar-Ar age data, A- and B- Groups were intruded during $64{\sim}52\;Ma$ and $51{\sim}44\;Ma$, respectively. The result means that the direction of tensional stress in and around the SE Korean peninsula was changed abruptly from NNE-SSW to $EW{\sim}WNW-ESE$ at about 51 Ma. Considering the tectonic environments during the Paleogene, it is interpreted that A-Group was injected along the WNW-trending tensional fractures developed under an regional sinistral simple shear regime which was caused by the north-northwestward oblique subduction of the Pacific plate beneath the Eurasian plate. Meanwhile, the regional stress caused by the collision of India and Eurasia continents at about 55 Ma was likely propagated to the East Asia at about 51 Ma, and then the East Asia including the Korean peninsula was extruded eastwards as a trench-rollback and the dip of downgoing slab of the Pacific plate was abruptly steepened. As a result, the strong suction-force along the plate boundary produced a tensional stress field trending EW or WNW-ESE in and around the Korean peninsula, which resultantly induced B-Group to intrude passively into the study area.

• Journal of Life Science
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• v.26 no.6
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• pp.698-704
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• 2016

The intracellular transport of organelles and protein complexes is mediated by kinesin superfamily proteins (KIFs). The first kinesin, kinesin 1, was identified as a molecular motor protein that moves various organelles and protein complexes along the microtubule rails in cells. Kinesin 1 is a tetramer of two heavy chains (KHCs, also called KIF5s) and two kinesin light chains (KLCs). KIF5s interact with many different proteins through their tail region, but their binding proteins have not yet been fully identified. To identify the interaction proteins for KIF5A, we performed yeast two-hybrid screening and found a specific interaction with ferritin heavy chain (Frt-h), which has a role in iron storage and detoxification. Frt-h bound to the amino acid residues between 800 and 940 of KIF5A and to other KIF5s in the yeast two-hybrid assay. The coiled-coil domain of Frt-h is essential for interaction with KIF5A. In addition, ferritin light chain (Frt-l) interacted with KIF5s in the yeast two-hybrid assay. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to KHC specifically co-immunoprecipitated Frt-h and Frt-l from mouse brain extracts. These results suggest the kinesin 1 motor protein may transport the ferritin complex in cells.

• Journal of Life Science
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• v.22 no.12
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• pp.1672-1679
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• 2012

The effect of high stocking density (HSD) on the expression of stress and lipid metabolism associated genes in the liver of broiler chickens was examined by chicken genome array analysis. The chickens in a control group were randomly assigned to a $495cm^2/bird$ stocking density, whereas the chickens in a HSD group were arranged in a $245cm^2/bird$ stocking density with feeding ad libitum for 35 days. The chickens assigned to the HSD group had a significantly lower body weight, weight gain, and feed intake compared with those of the control group (p<0.05). The mortality of chickens was higher in the HSD group than in the control group. The microarray analysis indicated up-regulation of stress associated genes such as HMGCR, $HSP90{\alpha}$, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, and down-regulation of interferon-${\gamma}$ and PDCD4 genes. The endoplasmic reticulum stress associated genes, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, were highly expressed in the HSD group. The genes, ACSL5, TMEM195 and ELOVL6, involved in fatty acid synthesis, were elevated in the HSD group. The genes, ACAA1, ACOX1, EHHADH, LOC423347 and CPT1A, related to fatty acid oxidation, were also activated in the HSD group. These results suggest that a HSD rearing system stimulates the genes associated with fatty acid synthesis as well as fatty acid oxidation in the liver of broiler chickens.

• Journal of Life Science
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• v.22 no.12
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• pp.1658-1664
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• 2012

Conjugated linoleic acid (CLA) exhibits several beneficial biological activities including anticarcinogenesis and body-fat reduction. Now, we report that CLA ameliorated the oxidative stress in rat cardiomyoblast cells, H9c2, treated with hydrogen peroxide ($H_2O_2$). Cells were cultured in DMEM/F-12 media at $37^{\circ}C$ with humidified atmosphere of 5% $CO_2$. The cells, cultured for 48 hrs, were seeded at a density $3.5{\times}10^3$ cell/well in a 24 well-plate and incubated for 24 hr. Using these cells, two experiments were performed: the cytotoxicity test of CLA (10, 20, 30, 40, and $50{\mu}Ms$), and the oxidative stress amelioration test of CLA (20 and $50{\mu}Ms$) against cells treated with $H_2O_2$ (10 and 50 ${\mu}Ms$) for 1 and 2 hrs. CLA enhanced the growth of H9c2 cells at any concentrations of CLA and at any incubation times (up to 6 days), indicating that CLA acts as a growth stimulant. No protective effect of CLA (20 and $50{\mu}Ms$) was seen in cells treated $50{\mu}M$ $H_2O_2$ for 1 and 2 hr, but these CLA concentrations ameliorated (p<0.05) the adverse effect of $10{\mu}M$ $H_2O_2$ in cells treated for 1 hr. These CLA concentrations significantly (p<0.05) reduced the proportion of apoptotic cells, relative to control cells. These results suggest that CLA protected H9c2 cells from the oxidative stress of $H_2O_2$ through the suppression of cell apoptosis and could be a useful compound for the prevention of cardiac diseases caused by oxidative stress.

• Journal of the Korean earth science society
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• v.39 no.5
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• pp.419-435
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• 2018

The purpose of this study is to understand meteorological and climatological factors that have influence on the garlic product in Seosan and Taean, and to analyze the economic value according to the use of climatical information data for garlic farmers. The climatological characteristics and trends in this area are analyzed using the meteorological data at the Seosan local meteorological agency from 1984 to 2013, the national statistical data for the product of garlic from 1989 to 2013, and the scenario data for climate change (RCP 4.5 and 8.5) for the period from 2001 to 2100. The results are as follows. First, the condition of lower temperature for garlic growth in winter season is satisfied with the mean air temperature. The wind speed are lower and stronger in Seosan and Taean than other garlic area. The suitable condition for the growth of northern type of garlic shows the decreasing trend in the accumulated precipitation in May. However, the area of growing the northern type garlic in the future is likely diminished because mean air temperature, accumulated precipitation, and mean wind speed are strong in the harvest time of garlic. Second, the seedtime of the northern and southern type of garlic using climate change scenarios (RCP 4.5, 8.5) in Seosan and Taean is getting late as time passes. and the harvest time gets faster, which indicates s that the period of garlic cultivation becomes shorter from 50 days to around 90 in the next 100 years. Third, the beginning days of white rot and delia platura of garlic are estimated by applying to the meteorological algorithm using mean air temperature and soil humidity. Especially, the beginning day of white rot garlic is shown to be faster according to the scenarios of RCP 4.5 and RCP 8.5. Fourth, the product of garlic (kg/10a) shows a high correlation with the minimum air temperature of a wintering time, the mean wind speed of a wintering time, the accumulated precipitation of a corpulent time, and the mean relative humidity of corpulent time of garlic. On the other hand, the analysis of garlic product when using the meteorological information data in cultivating garlic in Seosan and Taean reveals that the economic value increases up to 9% in total.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.10
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• pp.1528-1534
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• 2010

The antibacterial effects of seed decontamination during presoaking before sprouting as an intervention step for eliminating foodborne pathogens on red radish seeds were evaluated. The effect of seed decontamination on seed germination rate was also evaluated. Red radish seeds were inoculated (at a level of 3 to 4 log CFU/g) with Listeria monocytogenes ATCC 19111 and decontaminated with 20,000 ppm calcium hypochlorite, 50 and 100 ppm chlorinated water, acidic electrolyzed water, low-alkaline electrolyzed water, and ozonated water for 6 hours. The control seeds were immersed in distilled water. The germination rate was measured on each treatment for 48 hours. Treatments with 20,000 ppm calcium hypochlorite, acidic and low-alkaline electrolyzed water were more effective than treatments with chlorinated water and ozonated water. Immersion in 20,000 ppm calcium hypochlorite resulted in the largest microbial reduction (more than 3 logs). Treatments with acidic and low-alkaline electrolyzed water reduced APC by 3 logs and L. monocytogenes counts by 2 logs. After sprouting, APC and L. monocytogenes counts on seeds treated with 20,000 ppm calcium hypochlorite, acidic and low-alkaline electrolyzed water were significantly lower than the control. The germination rate ranged from 93.5% to 97.7% except for 20,000 ppm calcium hypochlorite (from 82.3% to 84.8%) after 48 hours. Although the treatments tested in this study will not eliminate L. monocytogenes on inoculated red radish seeds, the results show that rapid growth of surviving cells during sprouting could be prevented if red radish seeds are given a presoak treatment used in combination with a disinfectant treatment of irrigation water.

• Journal of Gastric Cancer
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• v.8 no.4
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• pp.256-261
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• 2008

Purpose: Intra-abdominal fluid collection is one of the risk factors associated with postoperative morbidity or mortality for patients who undergo gastric cancer surgery. The aim of this study was to analyze the clinicopathological characteristics of the patients with postoperative intra-abdominal fluid collection and to identify the indications for inserting a percutaneous drain (PCD) in patients with intra-abdominal fluid collection. Materials and Methods: Among the 1,277 patients who underwent operations for gastric cancer at Seoul National University Hospital between April 2005 and July 2006, the data of 117 patients with an intra-abdominal fluid collection were reviewed. Results: The number of patients' with pathologic stage I, II, III and IV disease was 42 (36.8%), 23 (20.2%), 16 (14%) and 33 (28.9%), respectively. Forty-three patients (36.3%) underwent PCD insertion and the other 43 patients received conservative management. A univariate analysis of multiple clinical variables revealed that age, gender, diabetes, liver disease, lymph node dissection, the pathologic stage and the body mass index (BMI, $kg/m^2$) were not significantly associated with PCD insertion (P>0.05). However, the univariate analysis showed that two characteristics were associated with a significantly high incidence of PCD insertion: a diameter of an intra-abdominal fluid collection greater than 4 cm and infectious signs such as leukocytosis, fever and bacteremia. Conclusion: About two thirds of the intra-abdominal fluid collections after surgery for gastric cancer were managed with only conservative method without other morbidities of mortality. Surgeons should consider performing PCD insertion if the largest diameter of an intra-abdominal fluid collection is over 4 cm or if infectious signs are seen.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.35 no.1
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• pp.36-47
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• 2017

In this research, sematic comparative study on formative idea and landscape elements composition was made between Soswaewon which was a remote villa garden in Joseon Dynasty and Canglang Pavilion which was built at a time when Neo-confucianism had been created in Song dynasty. From which the summary was resulted as below: Firstly, Soswaewon was fully devoted to Neo-confucianism among Korean remote villa gardens, Canglang-Pavilion was deeply affiliated with Neo-confucianism the deep-rooted thought. Adding a comment, as a richly historied Chinese private garden it was the best preserved even now and the farmost point from now and approximately 500 years advanced than Soswaewon, moreover Sosunheum the builder belonged to gentry society in Song dynasty. Secondly, Both Soswaewon and Canglang-Pavilion were remarkable thank to nature friendly concept which was deeply rooted in the eremitic thought(隱逸思想). Most of all, it might be interpreted the location of Soswaewon lay down in a mountain, as to be swallow hidden(小隱) but that of Canglang-Pavilion lay down in a city, as to be middle hidden(中隱). Thirdly, approaching with comparative study to gardens' factors implicated in symbolization when naming both Soswaewon and Canglang-Pavilion, neo-confucianism in Song dynasty in which diverse thoughts ranging Confucianism and Buddhism and Taoism had formed ensemble and developed was deeply built in Soswaewon, as to be in consistency to nature and to be a basis for comprehending diverse symbolization factors found in Canglang-Pavilion. Fourthly, given that forms or factors of gardens was tangibly came up with thoughts' variation we surely underwent to comparative study. Through which we could know Soswaewon was made by maximizing use of nature but minimizing addition of artificial things. In other words, it was trial of expressing semantic feature of the site in a way blending natural circumstance and liberal one. One of the representatives, Daebongdae in Soswaewon was a site where the owner's idea came up with. Scenary was recreated and nature was represented in interspace of Canglang-Pavilion with the high wall around the border, for which it renounced the world. To the end, it was understood there was the location characteristic of Canglang-Pavilion which let us look down from mountain and take some time for introspection. It might be said that the cultural root was in common between Korea and China, however it was found there were lots of differences in forms and features of gardens. We were able to interpret that social and cultural background were led to gardens' formation in which individual characteristic of two nations were blended, from which difference was resulted.

• The Journal of Engineering Geology
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• v.25 no.4
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• pp.557-576
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• 2015

Groundwaters in granite, gneiss, and two-mica granite formations, including faults, in the Hoengseong area are examined to determine the relationship between their uranium and radon-222 contents and rock types. The chemical compositions of 38 groundwater samples and four surface water samples collected in the study area were analyzed. Sixteen of the samples showing high uranium and radon-222 contents were repeatedly analyzed. Surface radioactivities were measured at 30 points. The uranium and radon-222 concentrations in the groundwater samples were in the ranges of 0.02-49.3 μg/L and 20-906 Bq/L, respectively. Four samples for uranium and 35 samples for radon had concentrations exceeding the alternative maximum contaminant level of the US EPA. The chemical compositions of groundwaters indicated Ca(Na)-HCO₃ and Ca(Na)-NO₃(HCO₃+Cl) types. The pH values ranged from 5.71 to 8.66. High uranium and radon-222 contents in the groundwaters occurred mainly at the boundary between granite and gneiss, and in the granite area. The occurrence of uranium did not show any distinct relationship to that of radon-222. The radon-222, an inert gas, appeared to be dissolved in the groundwater of the aquifer after wide diffusion along rock fractures, having been derived from the decay of uranium in underground rocks. The results in this study indicate that groundwater of neutral or weakly alkaline pH, under oxidizing conditions and with a high bicarbonate content is favorable for the dissolution of uranium and uranium complexes such as uranyl or uranyl-carbonate.