• Animal Bioscience
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• 제34권5호
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• pp.789-800
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• 2021

Objective: Conservation and genetic improvement of cattle breeds require information about genetic diversity and population structure of the cattle. In this study, we investigated the genetic diversity and population structure of the three cattle breeds in the Korean peninsula. Methods: Jeju Black, Hanwoo, Holstein cattle in Korea, together with six foreign breeds were examined. Genetic diversity within the cattle breeds was analyzed with minor allele frequency (MAF), observed and expected heterozygosity (HO and HE), inbreeding coefficient (FIS) and past effective population size. Molecular variance and population structure between the nine breeds were analyzed using a model-based clustering method. Genetic distances between breeds were evaluated with Nei's genetic distance and Weir and Cockerham's FST. Results: Our results revealed that Jeju Black cattle had lowest level of heterozygosity (HE = 0.21) among the studied taurine breeds, and an average MAF of 0.16. The level of inbreeding was -0.076 for Jeju Black, while -0.018 to -0.118 for the other breeds. Principle component analysis and neighbor-joining tree showed a clear separation of Jeju Black cattle from other local (Hanwoo and Japanese cattle) and taurine/indicine cattle breeds in evolutionary process, and a distinct pattern of admixture of Jeju Black cattle having no clustering with other studied populations. The FST value between Jeju Black cattle and Hanwoo was 0.106, which was lowest across the pair of breeds ranging from 0.161 to 0.274, indicating some degree of genetic closeness of Jeju Black cattle with Hanwoo. The past effective population size of Jeju Black cattle was very small, i.e. 38 in 13 generation ago, whereas 209 for Hanwoo. Conclusion: This study indicates genetic uniqueness of Jeju Black cattle. However, a small effective population size of Jeju Black cattle indicates the requirement for an implementation of a sustainable breeding policy to increase the population for genetic improvement and future conservation.

• 한국축산식품학회지
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• 제39권3호
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• pp.402-409
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• 2019

Jeju black cattle are known as one of Korea's traditional cattle. However, Hanwoo is more well-known to Korean meat consumers as representative beef cattle. Despite the popularity of these two breeds, comparison of the nutritional characteristics between Jeju black cattle and Hanwoo have not been studied. Here, we compared the fatty acid and amino acid characteristics between two Korean traditional cattle and Wagyu breeds. A total of 62 cattle were used in this study. The Jeju black cattle beef had significantly higher unsaturated fatty acids than Hanwoo (p<0.05). Savory fatty acids, including oleic acid were also higher than in Hanwoo cattle (p<0.05). The negative flavor fatty acids, such as palmitic acid were significantly lower than in Hanwoo (p<0.001). On the other hand, linoleic acid which imparts a negative flavor was higher than Hanwoo (p<0.05). Amino acids, including alanine and glutamine, usually representative of the umami taste were present in significantly higher proportions in Jeju black cattle (p<0.05). In addition, bitter tasting amino acids, including valine, leucine, isoleucine, and methionine were lower in Jeju black cattle beef than in Hanwoo (p<0.05, p<0.001, p<0.001, and p<0.001 each). Taken together, our results suggest that Jeju black cattle beef had higher savory flavor and umami taste which affected consumers preference for the meat.

• 생명과학회지
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• 제24권8호
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• pp.910-914
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• 2014

본 연구는 현행 한우확인시험법에 이용되는 Microsatellite (MS)와 소의 유전형질 중 품종 판별에 주로 이용되는 Melanocortin Receptor 1 (MC1R) 유전자상의 Single Nucleotide Polymorphism (SNP)를 분석하여 설계한 Primer를 Multiplex PCR을 활용하여 소의 품종을 판별하였다. MC1R 유전자형은 $E^D$, $E^+$, e형의 3Type으로 나뉘며 $E^D/E^D$, $E^D/E^+$, $E^D/e$, $E^+/E^+$, $E^+/e$, e/e의 6가지 유전자형을 가진다. $E^D$유전자형은 외래종이 지닌 유전자형으로 $E^D/E^D$, $E^D/E^+$, $E^D/e$이 이에 속하며, e유전자형은 한우가 지닌 유전자형으로 e/e의 유전자형을 가진다. 흑한우의 경우 $E^D$, $E^+$, e의 모든 유전자형을 가지고 있으나 이는 교잡에 의해 나타난 것으로 보이며 $E^+$유전자형이 흑한우 고유의 유전자형으로 추정되어 본 연구에서는 이에 따라 $E^+/E^+$, $E^+/e$의 유전자형을 흑한우로 분류하였다. 그러나 $E^D$, $E^+$의 경우 단순 PCR기법만으로는 그 판별에 어려움이 있어, MS Maker를 활용한 다형성 분석을 통해 흑한우와 수입우를 판별할 수 있는 새로운 Primer를 설계하였으며, 이를 통해 소의 품종을 판별하였다.

• 동아시아식생활학회지
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• 제22권4호
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• pp.497-505
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• 2012

냉장한 제주흑우육, 한우육 및 호주산 수입우육의 품질 특성을 비교하였다. 영양적 특성에서, 제주흑우육의 수분은 65.91%, 조단백질은 17.39%로 가장 낮고 조지방은 15.89%, 조회분은 0.79%로 가장 높게 나타났으며, 조회분은 유의적 차이를 보이고 나머지는 모두 시료 간에 유의적 차이가 없었다. 제주흑우육은 철과 아연 함량이 각각 29.85 및 48.13 ppm으로 한우육보다 낮았으나 유의적 차이가 아니고 호주산 우육보다 많게 나타났으며(p<0.05), 칼슘, 인, 나트륨 및 구리의 함량은 시료 간 유의적 차이가 없는 것으로 분석되었다. 제주흑우육은 아미노산 총량과 필수 아미노산 함량이 각각 16.81 및 9.08%로 다른 시료에 비해 가장 낮았으나 시료 간에 유의적 차이가 나타나지 않았으며, 총아미노산에 대한 필수아미노산의 비율은 54.01%로 가장 높게 나타났다. 제주흑우육은 포화지방산 총량(43.25%), stearic acid(13.51%), linoleic acid(2.39%) 및 필수지방산(2.47%) 함량이 가장 낮은 반면 불포화지방산 총량(56.69%), 단가불포화지방산인 oleic acid 함량(47.80%) 및 MUFA/SFA 비율이 높게 나타나서 호주산 우육에 비해 유의적 차이가 있었다. PUFA/SFA 비율은 시료 간에 유의적 차이가 없었다. 물리화학적 특성에서, 제주흑우육은 적색도($a^*$)가 높고 융점이 가장 낮게 나타났으며(p<0.05), 명도($L^*$)와 황색도($b^*$), pH, 보수력, 가열 감량 및 전단력은 시료 간에 유의적 차이가 없었다. 관능평가 결과에서, 제주흑우육은 생육의 향, 가열육 향 및 기호도가 가장 우수하여 호주산 우육에 비교하면 유의성이 인정되었다(p<0.05). 그러나 가열육의 맛, 다즙성 및 연도는 시료 간에 유의적 차이가 나타나지 않았다.

• Molecules and Cells
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• 제20권3호
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• pp.325-330
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• 2005

Ancient cattle bones were excavated from archaeological sites in Jeju, Korea. We used molecular genetic techniques to identify the species and establish its relationship to extant cattle breeds. Ancient DNA was extracted from four sources: a humerus (Gonae site, A.D. 700-800), two fragments of radius, and a tooth (Kwakji site, A.D. 0-900). The mitochondrial DNA (mtDNA) D-loop regions were cloned, sequenced, and compared with previously reported sequences of various cattle breeds (9 Asian, 8 European, and 3 African). The results revealed that these bones were of the breed, Bos taurus, and a phylogenetic tree indicated that the four cattle bones formed a monophyletic group with Jeju native black cattle. However, the patterns of sequence variation and reports from archaeological sites suggest that a few wild cattle, with a different maternal lineage, may have existed on Jeju Island. Our results will contribute to further studies of the origin of Jeju native cattle and the possible existence of local wild cattle.

• 생명과학회지
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• 제27권7호
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• pp.760-766
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• 2017

본 연구는 Lysophospholipase I (LYPLA1) 유전자의 SNP 마커인 rs385360448 g.23655332G>C의 유전자형이 제주도 한우와 제주흑한우(한우${\times}$제주흑우, JCC) 집단의 도체형질에 미치는 영향을 시험하였다. 한우 거세우 집단에서 LYPLA1 rs385360448 G/-를 보유한 도체는 C/C 동형접합에 비해 유의적으로 높은 수준의 근내지방도(MARB)와 조직감지수(TI)를 나타내었다(p<0.05). 반면, LYPLA1 유전자형은 도체중, 등지방두께, 등심단면적, 육색, 지방색에서는 유의적인 차이를 보이지 않았다(p>0.05). JCC 거세우에서는 LYPLA1 G/- 도체에서 C/C 동형접합에 비해 등심단면적(EMA)의 수준이 유의적으로 더 높았다(p<0.05). 이번 연구의 결과들은 LYPLA1 유전자형이 소의 등심에서 인지질 대사를 통해 근내지방도와 조직감의 수준과 등심단면적의 수준을 변화시킬 수 있음을 보여주고 있다. 이러한 결과들은 LYPLA1 유전자형이 한우와 제주흑우-유래의 산업화축군에서 도체형질을 개선할 수 있는 유전자 마커로써의 기능을 수행할 수 있을 것으로 기대된다.

• 한국수정란이식학회지
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• 제21권3호
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• pp.191-198
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• 2006

본 연구는 $2001{\sim}2005$년(5년간) 제주도에서 사육중인 제주 한우와 흑우를 체내 수정란의 생산과 수정란 이식 기술을 통하여 조기 증식하고자 실시하였다. 한우 고등 등록우 286두와 흑우 경산우 69두(총 355두)에 대하여 발정 주기에 관계없이 CIDR를 질내에 삽입 후 7일째부터 성선 자극 호르몬($Folltropin^{(R)}-V$) 400 mg을 50 mg씩 균등하게 나누어 4일간 12시간 간격으로 근육주사하였다. 투여 6회째에 CIDR를 제거하였으며, 동시에 $PGF_{2{\alpha}}$ 25mg을 근육 주사하여 과배란을 유기하였다. 공란우의 인공수정은 $PGF_{2{\alpha}}$투여 후 발정을 확인하고 12 시간 간격으로 3회 인공 수정하였으며, 2회 인공 수정시 GnRH $250{\mu}g$을 근육 주사하였다. 수정란 회수는 1차 인공 수정 후 $7{\sim}8$일째에 비외과적 방법으로 채란하였다. 수란우는 CIDR를 7일간 삽입하였다가 제거하는 동시에 $PGF_{2{\alpha}}$ 25 mg을 근육 주사하여 공란우와 발정을 동기화 시켰다. 수정란 이식은 발정이 동기화된 수란우 1,219두에 대하여 6명의 수정란 이식 시술자가 비외과적 방법으로 황체가 존재하는 자궁각에 이식하였다. 한우 및 흑우 공란우의 평균 회수 총 난자수 및 이식 가능 수정란 수는 각각 7.4개 및 4.7개 였으며, 회수 총 난자수는 2 품종간에 차이가 없었으나, 이식 가능 수정란 수는 한우(5.0개)가 흑우(3.5개)에 비해 많았다(p<0.05). 공란우의 반복 과배란 처리에 따라 흑우에서는 과배란처리 두수 대비 수정란 채란우 비율이 감소되었으며(p<0.05), 한우에서는 회수 총 난자수 및 이식 가능 수정란 수의 감소가 나타났다(p<0.05). 과배란 처리 계절에 따른 수정란 회수 성적은 여름(5.6개)이 겨울(2.9개)에 비해 이식 가능 수정란의 수가 많았다(p<0.01). 수정란 이식 후 수태율은 평균 40%를 나타내었으며, 공란우의 품종간 차이가 없었으며, 이식년도가 2001년에서 2004년으로 경과될 때 수태율이 증가하였으며(p<0.05), 수정란 이식 시술자에 따른 수태율의 차이가($33.0{\sim}45.9%$) 인정되었다(p<0.01). 본 연구의 결과는 제주 한우 및 흑우에서 CIDR를 이용한 과배란 처리 및 수란우의 발정동기화 처리는 한우 체내 수정란의 안정적인 생산과 수란우의 준비가 가능함을 보여 주었다.

• 한국수정란이식학회지
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• 제32권3호
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• pp.221-226
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• 2017

Spermatozoa viability can be assessed by microscopy, flow cytometry, and other methods using fluorescent stain. Flow cytometry can be used to examine the morphological and functional characteristics of spermatozoa in a short time. The purpose of this study was to compare the viability of cryopreserved spermatozoa in Jeju black cattle by two dual fluorescent stain methods. Semen of Jeju black cattle raised in Subtropical Livestock Research Institute, National Institute of Animal Science, RDA were collected with artificial vaginal technique. Sperm was diluted with $Triladyl^{(R)}$-egg yolk diluent and then was performed cryopreservation. There was no significant difference in viability of spermatozoa according to the two dual fluorescent stain methods. However, when the distribution of spermatozoa according to the staining method was compared, the spermatozoa group stained with 6-CFDA/PI was more clearly distinguished than the spermatozoa group stained with calcein AM/PI.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.77-83
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• 2011

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

• 한국수정란이식학회지
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• 제27권3호
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• pp.149-154
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• 2012

This study was carried out to investigate effective condition for producing somatic cell nuclear transfer (SCNT) embryos of Jeju native cattle. As donor cells for SCNT, ear skin cells from Jeju native cattle were used. In experiment 1, the effect of recipient oocyte sources on the development of Jeju native cattle SCNT embryos were examined. Fusion rate of recipient oocyte and donor cell was not different between the Hanwoo and Holstein recipient oocytes (86.0% vs 89.9%). The rate of embryos developing to the blastocyst stage was significantly (p<0.05) higher in Hanwoo recipient oocytes than in Holstein recipient ones (28.2% vs 14.7%). Blastocysts derived from Hanwoo recipient oocytes contained higher numbers of total cells than those derived from Holstein ones ($115.1{\pm}40.8$ vs $101.4{\pm}33.3$), although there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the sources of recipient oocytes. In experiment 2, the development of Jeju native cattle and Hanwoo SCNT embryos were compared. Hanwoo oocytes were used as the recipient oocytes. Fusion rate was not different between the Jeju native cattle and Hanwoo SCNT embryos (92.1% vs 92.9%). The blastocyst rate of SCNT embryos was significantly (p<0.05) lower in Jeju native cattle than in Hanwoo (16.9% vs 31.0%). Blastocysts derived from Jeju native cattle SCNT embryos contained smaller numbers of total cells than those derived from Hanwoo ones ($136.6{\pm}33.7$ vs $149.9{\pm}39.7$), but there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the Jeju native cattle and Hanwoo SCNT embryos. The present study demonstrated that Hanwoo recipient oocytes were more effective in supporting production of Jeju native cattle SCNT embryos, although Jeju native cattle SCNT embryos showed reduced developmental capacity when compared to Hanwoo SCNT embryos.