• 대한수의학회지
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• 제47권2호
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• pp.175-185
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• 2007

This study was carried out to investigate the pathogenesis and pathogenicity of the porcine circovirus type 2 (PCV2) Korean isolate from weaned pigs. Twenty four weaned pigs, PCV2, porcine reproductive and respiratory syndrome virus (PRRSV) and porcine parvovirus (PPV) antibodies free, were allocated to 4 groups (n = 6). Six pigs were inoculated intranasally with PCV2 alone, 6 with PCV2 and PRRSV, 6 with the combined PCV2/PRRSV/PPV inoculum, and 6 were remained as a uninoculated negative control. Pigs were killed 3 and 6 weeks after inoculation and tissue samples examined for gross and microscopic lesions and for the presence of PCV2 antigens and nucleic acids. Experimentally inoculated pigs were evaluated for 3 considerations: 1. development of postweaning multisystemic wasting syndrome (PMWS), 2. distribution of viral antigens by immunohistochemistry and polymerase chain reaction (PCR), and 3. cytokine mRNA levels in lymph nodes. Pigs inoculated with PCV2/PRRSV/PPV showed typical clinical signs, gross findings, and histopathologic characteristics of PMWS. In the PCV2/PRRSV/PPV inoculated group, the PCV2 antigen was widely distributed in various parenchymal organs such as brain, spinal cord, tonsil, lymph nodes, lung, heart, liver, kidney, spleen, and peyer's patch. Lymph node mRNA expression of IL-$1{\alpha}$, IL-2R and IL-8 was determined by real-time PCR. The pigs of PCV2/PRRSV and PCV2/PRRSV/PPV inoculation group, the mRNA expression was characterized by a decrease of IL-$1{\alpha}$, IL-2R and IL-8. The decrease of cytokine mRNA represent the state of T cell immuno-suppression in pig, and nicely support the evidence for the impairment of immune system in pigs with PMWS. In conclusion, PCV2 infection and some additional infectious causes such as PRRSV and/or PPV are warranted for the presence of PMWS in weaned pigs in Korea.

• 한국식품조리과학회지
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• 제20권2호
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• pp.180-186
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• 2004

느릅나무(Ulmus cotex) 근피의 추출액을 농도별로 첨가한 후 식빵의 품질특성을 기계적, 관능 검사 및 저장기간별 미생물수의 변화를 관찰한 결과, 느릅나무 추출액이 농도를 증가함에 따라 식빵의 수율은 증가하였으나 반죽의 부피와 수율 그리고 비용적은 유의적인 차이가 관찰되지 않았다. 식빵의 표면 색도 중 L값과 내부색도 a 값은 느릅나무 추출액의 농도의 증가함에 따라 유의적으로 증가하였으나, 내부색도 L값에는 추출액 농도의 증가에 따라 각 군간의 유의적인 차가 관찰되지 않았다. 식빵의 기계적인 검사에서 경도, 검성은 느릅나무 추출액을 30% 첨가한 식빵군이 가장 높았으나, 검성은 추출액의 농도가 증가함에 따라 유의적으로 증가하였다. 관능검사에서는 flavor와 조직감 및 전반적인 기호도에서 느릅나무 10% 추출액 첨가 식빵에서 제일 우수하게 나타났으나 추출액의 농도를 20%이상 증가시킴에 따라 식빵의 색과 기호도는 유의적으로 감소하였다. 일반적으로 관능검사와 기계적 검사간에 부의 상관관계를 나타내었으며, 특히 전반적인 기호도와 경도, 응집성, 탄력성, 검성 등의 기계검사 간에 부의 상관관계가 관찰되었다. 저장기간에 따른 일반 세균수의 변화는 저장 초기에는 각 군의 유의적인 차이가 없었으나 6일 경과 후 추출액의 증가에 따른 세균수의 감소가 관찰되었으며, 곰팡이는 느릅나무 추출액 첨가 식빵에서는 저장 3일까지는 관찰되지 않았고 저장기간이 연장됨에 따라 현저한 증가가 나타나지 않아 느릅나무 추출액의 탁월한 항균 활성을 입증하였다.

• 한국수자원학회논문집
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• 제45권6호
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• pp.607-616
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• 2012

본 연구는 AWS 관측강우정보를 이용하여 실시간 유량예측을 수행할 경우 적용가능한 예측선행시간 및 정확도를 평가하고자 하는데 그 목적이 있다. 이를 위해 남한강 상류유역을 대상유역으로 선정하였으며 2006~2009 홍수기간에 대해 SURF 모형을 구축하였다. 관측유량 자료동화 수행 유무에 따른 모의유량은 관측유량을 잘 모의하며 유효성지수를 이용하여 자료동화 효과를 분석한 결과에서 충주댐 32.08%, 달천 51.53%, 횡성 39.70%, 여주 18.23%가 개선된 것으로 나타났다. 첨두유량 발생시간 이전 가상의 현재시점까지의 AWS 관측강우정보를 이용하여 유량예측 적용성을 평가한 결과 허용오차 20% 범위 내에서 첨두유량은 충주 11시간, 달천 2시간, 횡성 3시간, 여주 5시간, 유출용적은 충주 13시간, 달천 2시간, 횡성 4시간, 여주 9시간 이내에서 예측이 가능한 것으로 나타났다. 따라서 유역의 지체효과로 인해 관측강우만을 이용하여 적정 예측시간에 대해서 실시간 첨두유량 예측이 가능할 것으로 판단된다.

• Pediatric Infection and Vaccine
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• 제19권2호
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• pp.61-70
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• 2012

목적: 본 연구는 단일 기관에서 소아암 환자의 2009 인플루엔자 A(H1N1) [A(H1N1)pdm09] 감염 양상을 조사하고 이전의 계절 인플루엔자 감염과 비교 분석하고자 하였다. 방법: 삼성서울병원에서 2009년 8월부터 2010년 2월까지 A(H1N1)pdm09 감염, 2000년 1월부터 2009년 5월까지 계절 인플루엔자 A 감염이 확진된 소아암 환자의 의무기록을 후향적으로 분석하였다. 결과: 82명의 소아암 환자에서 A(H1N1)pdm09 감염이 확진되었다. 10명(12.2%)의 환자에서 하기도 감염증 또는 호흡기 외 감염이 발생하였다. 3명(3.7%)의 환자가 사망하였고 그 중 2명은 A(H1N1)pdm09 기여 사망이었다. 합병된 감염증과 관련하여 유의성을 가지는 위험인자는 감염의 시점(2009년 44-45주)과 병원 내 감염이었다. 이전의 계절 인플루엔자 A 감염과 비교하였을 때 임상적 특성에는 유의한 차이가 없었으나 A(H1N1) pdm09 감염에서 적극적인 항바이러스제 치료가 시행되었다. 결론: 소아암 환자에서 A(H1N1)pdm 감염은 경증부터 중증까지 다양한 임상 경과를 보였으며 이전의 계절 인플루엔자와 비교했을 때 임상 양상의 유의한 차이는 없었다.

• Journal of Preventive Medicine and Public Health
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• 제38권1호
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• pp.82-92
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• 2005

Objective : To evaluate the relationships among sociodemographic characteristics, health behaviors, levels of pros and cons and stages of change in Pap testing for uterine cervical cancer. Methods : A questionnaire survey was performed on 560 randomly sampled people who were assigned to participate in a Pap testing program by the National Cancer Screening Project in 2003' between 25 September and 10 October in Gyeonggi, Korea. Data about the behaviors and intentions of Pap testing, sociodemographic characteristics, health behaviors, and levels of acknowledged benefit (pros) and barrier (cons) for Pap testing was collected. The stages of change were grouped according to behaviors and intentions of Pap testing as passive, active, and relapse. Results : Logistic analysis between the passive and active groups showed that city dwellers, 'high' and 'middle' groups in terms of the individual s health belief, those who had undergone a health examination within the past 2 years, and those who had undergone hormone replacement therapy had a higher odds ratios to be in the active group. As the 'benefit' scores increased and the 'Unnecessity' scores decreased, the probabilities to be in the active group increased. According to the logistic analysis results between the active and relapse groups, those who were 60 years or older, members of the National Heath Insurance, and those who had not undergone a health examination within the past 2 years had a higher odds ratio to be in the relapse group. The 'Benefit' scores were not significant in this relationship. The probabilities of being in the relapse group increased as the Unnecessity and 'Shamefulness' scores increased. Conclusions : In conclusion, health planners should inform women in the passive group of the benefits and necessity of Pap testing. It would be better to reduce the barriers to the active group of undergoing Pap smear. This study might be a useful guide for future planning of Pap testing program.

• 터널과지하공간
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• 제19권4호
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• pp.287-303
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• 2009

한라산 정상부의 백록담 암체는 백록담조면현무암이 분포하는 동측구역과 한라산조면암이 분포하는 서측구역으로 구분된다. 백록담에서 진행되는 풍화, 암벽붕괴, 낙반은 전반적으로 백록담 서측구역에 분포하는 한라산 조면암의 풍화작용과 밀접한 연관성을 가지며, 동측구역에서는 개별 암석 블록들의 국지적 붕락현상이 발생한다. 본 연구에서는 한라산조면암 분포지역의 지질학적 특성을 조사하였으며, 풍화단계별로 조면암 시료를 채취하여 편광현미경분석, X-선 회절분석, 지화학적 분석, 공학적 특성시험을 수행하였다. SEM, XRD, XRF 분석을 통하여 2차 변질광물의 종류와 함량 및 광물조성 변화를 관찰한 결과 점토광물이 확인될 정도의 심한 화학적풍화작용은 발생하지 않았지만 풍화에 따른 화학적풍화지수와 주요원소의 함량 변화에 대한 분석 결과에 의거할때 화학성분의 용탈에 의한 미약한 화학적풍화작용이 발생한 것으로 판단된다. 풍화에 기인된 암석의 공학적특성변화를 고찰하기 위하여 밀도, 비중, 흡수율, 공극률, 탄성파속도, 일축압축강도, 슬레이크내구성지수, 동결-융해시험을 수행하였다. 인공풍화시험을 통한 균열발현 양상과 물리적 특성 측정 결과 기계적풍화작용은 미세균열 및 공극을 발달시켜 암반의 역학적 강도 저하를 야기하고 있으며, 기계적풍화 진행에 의한 추가적인 공극률 저하 양상을 고려할 때 $D_B$ = 1.5 또는 공극률 = $20{\sim}21%$ 범위에서 한라산조면암의 공학적 강도 특성이 소멸될 것으로 분석되었다.

• 한국습지학회지
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• 제14권2호
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• pp.159-168
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• 2012

도시화된 토지이용은 불투수율이 높고 강우시 다량의 비점오염물질이 유출되어 지표수 및 지하수에 영향을 끼친다. 이러한 포장지역의 비점오염물질로 인한 수질오염을 저감하기 위하여 환경부는 수질오염총량제도 및 개발사업에 대한 비점오염 저감시설 설치신고 제도를 도입하여 운영 중에 있다. 그러나 비점오염저감시설의 규모 및 저감기능 설계를 위해서는 유출특성 분석이 필요하나 현재 기초자료가 부족하여 원단위 산정 등의 애로점으로 남아있다. 따라서 본 연구에서는 원단위 산정 및 저감시설의 규모산정에 필요한 강우유출수 특성과 EMC를 제시하고자 한다. 모니터링은 도로 및 주차장에서 3년간 총 30개의 강우사상에 대하여 수행되었다. 초기강우 유출현상은 강우 시작 후 30분 동안 매우 분명하게 나타났으며, 유출수의 농도에 영향을 미치는 인자는 강우량, 유역면적, 토지이용, 지리 및 지형적 특성으로 분석되었다. 본 연구결과는 향후 포장지역의 원단위 산정 및 저감시설 설계시 기초자료로써 활용가능할 것으로 판단된다.

• 대한지역사회영양학회지
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• 제3권5호
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• pp.695-706
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• 1998

This study was designed to investigate the associations of the percent body fat dietary intake, plasma lipoprotein profile, lipoprotein(a), and plasminogen activator inhibitor-1(PAI-1) concentrations of 1982 Korean subjects(men : 1000 and women : 982) between the ages of 40 and 59 years. The dietary assessment consisted of twenty-four hour dietary records and food frequency questionnaires. The subjects were identified into one of the five rating groups of % body fat : lean, underweight, normal, overweight and obese groups. The biochemical assessment included measurements of plasma total cholesterol(TC), HDL-cholesterol(HDL-C), LDL-cholesterol(LDL-C), triglyceride(TG), lipoprotein(a)(Lp(a)), and PAI-1. With respect to the ratio of percent energy intake of carbohydrate : protein : fat of the normal group of the women was 62% : 17% : 20%, respectively. Women apparently had a higher intake of carbohydrates than men(52% : 17 : 20%) did. There was a linear relationship between energy intake and % body fat in both mean and women(with the exception of the underweight group of women). The relationship of % body fat of men to the protein and fat intake was higher than that of the carbohydrate intake. Of the men in the study, intakes of energy, protein and alcohol were positively correlated to % body fat. In women, energy, carbohydrate and protein intake were positively correlated to % body fat, however, the fat, cholesterol and alcohol intake did not show any correlation to the % body fat in women. This study showed that % body fat was positively correlated with plasma TC, LDL-C, PAI-1 levels, and TG, but the % body fat was negatively correlated with plasma HDL-C level in both men and women. These results indicated that the high energy intake of obese or overweight subjects might contribute to several of the biochemical indices fo coronary heart disease(CHD) risk. In conclusion, increased energy intake is associated with overweight or obesity in middle aged Korean people. There was no relationship between % energy intake of fat and % body fat in the study, in middle-aged Korean men and women. The plasma lipid profile and PAI-1 level thought to be the risk factors of CHD were positively associated with percent body fat in middle aged Korean people.

• 대한한방내과학회지
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• 제15권1호
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• pp.60-79
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• 1994

The studies were conducted to investigate the combined effects of Tonics and Mitomycin C(MMC). The effects of Tonics and MMC on the proliferation of Molt-4 cells, human leukemic cell line, and activation of human lymphocytes were estimated by MTT colorimetric assays. Selected medicines among 9 kinds of Tonics by results of MTT assays were treated with MMC in mice. The Tonics itself enhanced the proliferation of Molt-4, but the anti-proliferative effect of MMC was not intercalated by the combined treatment of Tonic and MMC. Inhibitory action of MMC was augmented by Sa Kun Ja Tang(SKT). This result was due to the inhibition of DNA synthesis. Among 9 kinds of Tonics, Sip Jean Dae Bo Tang(SDT), Saeng Maek San(SMS) and Kwi Bi Tang(KBT) did not inhibit the action of MMC, but activated lymphocytes. When the mice were treated by MMC, the number of leukocytes was decreased significantly at the 1st day, but recovered at the 7th day. In the groups of MMC treated with SDT or KBT, the number of leukocytes was increased significantly than the group of MMC treated only at the 3rd day. Combined treatment of the Tonics(SDT, SMS) and MMC retained the body weight of mice at the level of normal mice. SDT, SMS and KBT did not change the number of plaque forming cells(PFC), but MMC treated group decreased the number of PFC. The combined treatment of MMC and SDT increased the number of PFC significantly than the MMC treated group. SDT, SMS and KBT did not influence the proliferation of T cells, but MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and those tonics increased the T cell proliferation significantly than the MMC treated group. In conclusion, the results presented in this paper suggest that SDT, SMS and KBT can recover the side effects of MMC, such as weight loss, leukopenia and immunosuppresion, without any intercalating the anti-proliferative action of MMC in vivo.

• 한국축산식품학회지
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• 제28권5호
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• pp.667-674
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• 2008

This study was conducted to investigate the effects of germinated and fermented unmarketable soybean (GFS) on laying performance and egg quality in laying hens. A total of two hundred laying hens were divided into 5 groups (5 treatment $\times$ 4 replication $\times$ 10 birds each) and fed with the experimental diets for 8 wk as follows: control, GFS free; T1, GFS 0.15%; T2, GFS 0.3%; T3, GFS 1%; T4, GFS 2%. The laying performance, egg quality, blood profiles, cecal microbial population, isoflavone content in egg yolk were investigated. There were no significant differences laying performance, relative liver and spleen weights, egg yolk color, eggshell color among groups. Eggshell strength in groups fed with diets containing GFS increased, but not significantly. Eggshell thickness significantly increased in the GFS-supplemented group. No significant differences were observed in the blood profiles and intestinal microflora after supplementation. The decrease of Haugh unit during storage was alleviated by feeding of GFS (p<0.05). The concentrations of malondialdehyde in groups fed with GFS were decreased as compared with control (p<0.05). Isoflavones in the egg yolk were detected in group fed with diet containing 2% GFS. These results showed that unmarketable GFS could be used as a favorable feed additive and feedstuff for production of quality enhanced and isoflavone fortified eggs.