• Journal of Conservation Science
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• v.38 no.1
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• pp.64-71
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• 2022

Many investigations have been conducted on the biological damage and environmental conditions necessary to preserve the Janggyeong Panjeon and Daejanggyeongpan (woodblocks). We performed a survey on the concentration and diversity of airborne fungi in the Janggyeong Panjeon and compared them with the results of a survey from 2012. The temperature of the Beopbojeon building was slightly lower, while the relative humidity was higher than those found at the Sudarajang building. The concentration of airborne fungi in the Beopbojeon was 1.44-fold that of the Sudarajang. It was confirmed that the concentration and diversity of airborne fungi in the Janggyeong Panjeon differed depending on the sampling site. In total, 23 fungal genera were identified from the air samples, and 11 fungal and 1 bacterial genera were identified from the surface of the woodblocks. Among these, only five types of fungi were commonly distributed in the indoor air and surface of the Daejanggyeongpan; however, 58.3% of the fungi identified on the surface of the woodblocks were not observed in the in the air samples. The surface-dwelling fungi may accumulate dust to form microbial communities over time.

• Mycobiology
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• v.39 no.4
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• pp.306-309
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• 2011

Cladosporium cladosporioides H1 was found to be the most abundant microbe in Janggyeong Panjeon. C. cladosporioides H1 produced a 20 kDa xylanase, which was generally stable below $60^{\circ}C$ and had specialized activity in an acidic condition. Our results may lead to the development of a strategy for preservation of organic cultural heritage environments.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.198-204
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• 2011

This study was carried out to investigate occurence of microbiales density and characteristics of xylanase produced by those in Janggyeong Panjeon. Cladosporium cladosporioides H1, Penicillium citreonigrum H3, Penicillilum toxicarium H4, Aspergillus versicolor H6, Acremonium alternarium H7 isolated from Janggyeong Panjeon produced xylanase, which had different production rates and specialized activities in an acidic condition. Cladosporium cladosporioides H1, Aspergillus versicolor H6, and Acremonium alternatum H7 produced xylanase at a faster rate than other fungi. A xylanase of Cladosporium cladosporioides H1 and Penicillilum toxicarium H4 showed a high thermostability in an acidic condition. As results, this study may lead to the development of a strategy for preservation of organic cultural heritages.

• 보존과학연구
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• s.30
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• pp.157-170
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• 2009

We have evaluated the antifungal activities of oak vinegar to develop a natural biocide for organic cultural heritage. Fungi used in this study were screened from the cultural heritages, Kyujanggak and JanggyeongPanjeon and tested on organic substrates-degrading ability. In the results, 7 species of fungi have produced the extracellular enzymes to degrade CMC, xylan, lignin. Thus, we have used these seven species fungi to investigate the antifungal activity of oak vinegar in this study. In the result, the antifungal activity of oak vinegar indicated positive potencial. Especially, methylene chloride and ethylacetate fractions of the oak vinegar had high activities at the concentration of 5.0mg/disc. In these fractions, many different kinds of compounds such as phenolic and furfural, etc. were analyzed by GC-MS. The experiments indicated that the development of a biocide using natural extracts can have a potential to conserve of organic cultural heritages.

• 보존과학연구
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• s.33
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• pp.5-17
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• 2012

An Ola leaf manuscript, which consists of words carved on an Ola leaf and is filled up with a mixture of herbal oils made from Vateria acuminata Hyne and charcoal, is one of the native writing media in Sri Lanka. According to historical records, Ola leaf manuscripts had been used from the 1st to the 18th century A.D. From the recent findings that Ola leaf manuscripts have been preserved well for the past 400 years and are in good state of preservation, it is supposed that herbal oils preserve Ola leaves against environmental and biological factors such as fungi and insects. To evaluate the anti-biological susceptibility of the herbal oils, the molds isolated from wooden printing blocks in Janggyeong Panjeon of Haeinsa Temple in South Korea and bacteria and fungi isolated from Ola leaves were cultured. After spreading the microorganisms suspension on an agar plate, a disk paper containing a certain volume of herbal oil was placed on the agar plate. It was found in the experiment that herbal oil exhibits a clear zone, which is optically clear and inhibits the growth of microorganisms, against some molds and bacteria. The study results indicate that the herbal oil from the plant Vateria acuminata Hyne has the anti-bacterial and anti-fungal properties.

• Korean Journal of Heritage: History & Science
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• v.54 no.2
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• pp.194-215
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• 2021

Termites are important decomposers in the ecosystem. They are also economically significant structural pests. In this study, we reviewed the developments of termite control and recent research on termite management to provide information on the prevention and control of termites. In Korea, most of the damage to wooden historical buildings is caused by subterranean termites. Reticulitermes speratus kyushuensis is the main species, which is widely found throughout the country. In the early 1900s, inorganic insecticides, such as arsenic dust, were used for termite control. After the synthesis of chlorinated hydrocarbon pesticide in the 1940s, it was widely utilized and demonstrated high termite control efficacy. However, chlorinated hydrocarbon insecticides were later banned, disappearing from markets after reports emerged concerning environmental contamination and toxicity to humans. Therefore, the termite control industry sought a new termiticide; hence many pesticides were utilized for termite control. Organophosphate (1960s), carbamate (1970s), pyrethroid, and insect growth inhibitor (1980s) were newly synthesized and adopted. In the 1990s, the first commercial baits using chitin synthesis inhibitors (CSI) were developed, providing a means to eliminate an entire colony of subterranean termites around a structure. Many studies have been carried out on soil termiticides (liquid termiticides) and CSI baits to increase their efficacy, and different baits such as aboveground bait stations, fluid bait, and high-durability bait were also developed in the 2000s. In addition, the paradigm of termite control has shifted from localized treatments using soil termiticides to area-wide pest management using CSI baits to create termite-free zones and protect buildings over time. Termite infestations in wooden historical buildings in Korea have been reported since 1980, and considerable attention was drawn in the 1990s when several UNESCO world heritages such as the Jongmyo Shrine and the Janggyeong Panjeon Depositories of Haeinsa Temple were infested by subterranean termites. Since then, a survey of termite infestation in wooden architectural heritage has been conducted, and the National Research Institute of Cultural Heritage and Heritage Care Program regularly monitors those properties. Finally, we suggest termite management using primarily CSI baits, selective application of various soil treatments applied to the object, foundation soil treatment, research and development of durable termite baits, application of area-wide programs for wooden-building complexes, application of integrated termite management (ITM), and regular education for owners and managers to prevent and reduce termite damage.