• Korean Journal of Ichthyology
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• v.25 no.4
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• pp.200-207
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• 2013

We investigated developmental stages of egg and early life history of the Korean indigenous fish, Korean Chub Zacco koreanus from the South Han River in 2011 for phylogenetic study and conservation of this species. Eggs of Zacco koreanus were artificially fertilized by the dry method in the laboratory. The fertilized eggs were demersal, almost spherical in shape, transparent and unpigmented, with a pale yellow yolk, and no oil globule and average $3.09{\pm}0.07$ mm (n=10) in diameter. The hatching of the embryo took place in about 68 hrs after fertilization under water temperature of $20.0{\sim}23.0^{\circ}C$ and the newly hatched larvae were average $10.30{\pm}0.40$ mm (n=10) in total length (TL). Six days after hatching, the larvae grew up to $16.12{\pm}0.42$ mm (n=8) in TL and york sac absorption, mouth and anus opening were shown postflexion larvae stage. 17 days after hatching, most of fin-rays appeared at $18.21{\pm}0.38$ mm (n=6) in TL and brown spot appeared on the abdomen. 27 days after hatching, the larvae were brought up to $20.01{\pm}1.12$ mm(n=5) in TL and all their fin-rays were formed. 120 days after hatching, the larvae (juvenile) were $23.29{\pm}3.12$ mm (n=10) in TL and their body shape and color pattern were similar to the adult fish.

• Korean Journal of Ichthyology
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• v.29 no.2
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• pp.101-108
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• 2017

Egg development and early life history of the Korean natural monument fish Hemibarbus mylodon (Pisces: Cyprinidae) were investigated to provide basic data on biological characteristics and ecological recovery. Adult fish were collected from nature and transferred to the laboratory. For the first time, artificial maturation of females and males succeeded after 15 months of indoor culture. Mature eggs and sperm were obtained using Ovaprim injections (0.5 mL/kg) and then the eggs were fertilized using the dry method in the laboratory. The mature eggs were adhesive, turbid, and greyish; they averaged $2.21{\pm}0.06mm$ (n=30) in diameter. The embryos began to hatch about 78 h after fertilization at a water temperature $20{\pm}1^{\circ}C$, and the newly-hatched larvae were $6.6{\pm}0.75mm$ in total length (TL). At 14 days after hatching, the post-larval individuals were $13.5{\pm}0.23mm$ (TL), and their yolk sacs were completely absorbed. Twenty one days after hatching, they entered the juvenile stage and reached $13.5{\pm}0.23mm$ (TL). At 100 days after hatching, their band patterns, external form, and a pair of barbels were similar to those of adults, and they averaged $33.0{\pm}4.25mm$ (TL). The breeding technology and characteristics of early life history obtained in this study will be very helpful for conservation of H. mylodon in nature.

• Journal of Aquaculture
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• v.10 no.4
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• pp.417-424
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• 1997

This study was carried out to investigate the effects of experimental formulated diets, commercial diets and natural diet(dried Undaria) on growth and body composition of Haliotis discus hannai. Experimental diets was formulated to provide 34.1% protein and 7.5% lipid, and prepared by different $CaCl_2$ concentration (5, 10 and 15%) and dipping time (10, 20 and 60 second) in solution. The juvenile abalone average weighing 0.3g were distributed in a flow-through aquarium system using a cmpletely randomized desing with 13 diets and 3 replictes per diet (50 abalone/tank), and fed diets for 17 weeks. The abalone fed the time nine experimental formulated diets and two commercial diets showed better growth performance than that of natural diets. No significant differences were found among abalone fed the experimental diets and commercial diets in survival rate, weight gain, soft body weight and soft body composition (P>0.05).

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.123-131
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• 2012

This study analyzed on the characteristics of community structure, species composition, population conditions and annual mean radial growth of Taxus cuspidata in the subalpine zone of the Seoraksan, Deokyusan, and Hallasan National Parks. Deokyusan and Hallasan sites had three layers of stratification structure without tree layer in it and four layer in Seoraksan site. The major dominant species in the order of importance value were as follows: T. cuspidata, Acer tschonoskii var. rubripes, Quercus mongolica, Abies koreana and Sorbus commixta. The trees (> 5 cm DBH) of T. cuspidata were extremely high with 986.0 individuals/ha at the Hallasan site. Average DBH class were 42.0 cm at the Hallasan site and mainly showed large class. The populations of seedlings and saplings with 357.3 individuals/ha, and juvenile with 128.6 individuals/ha, as a succession tree, were found to be the highest at the Hallasan site. In the size frequency distribution, the populations of T. cuspidata in Mt. Halla site showed a reverse J-shaped curve and it was estimated that T. cuspidata community of this site might be maintained continuously as a stable state like present state. Annual mean radial growth of T. cuspidata populations at Seoraksan, Deokyusan, and Hallasan sites showed up as 1.27 mm/year, 0.93 mm/year and 0.89 mm/year respectively.

• Journal of Korean Home Economics Education Association
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• v.8 no.1
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• pp.1-10
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• 1996

This study aims at finding a new home economics education which will include male and female students as its teaching objects, and then providing home economics teachers with useful materials. For this purpose I examined analized male and female the upper secondary school students’and parents’recognition and demand to the home economics education The conclusion of this study is summarized as follows： 1. There was significant difference between male students(73.7％) and female students(89.8％) in the necessity for studying Home Economics(P＜.001). In the necessity of Home Economics education, male students emphasized the spiritual part but female students emphasized the functional part. In part of parents, there was no significant difference between fathers(95.5％) and mothers(96.4％). 2. The necessity of each part in the contents of Home Economics 1) In the part of family, there was significant difference between male and female students in the true nature of family, the role and responsibility of family, and the laws relating to family(P＜.001). There was significant difference between mothers and fathers in the true nature of family and the role and responsibility of family. Fathers and male students emphasized the true nature of family and the role and responsibility of family more than mothers and female students did. 2) In the Home management and economy parts, there was significant difference between male and female students in the significance of home management(P＜.001), the relation of family to society(P＜.001), and fluctuating measures for home economy. There was significant difference between fathers and mothers in the relation of family to society(P＜.05). Fathers and male students emphasized the relation of family to society more than mothers and female students did. Mothers, male and female students emphasized the importance of the protection of consumers. 3) In the clothes part, male students emphasized the role of clothes, the disign and making of clothes(P＜.001), the present situation of the resources of clothing and the distribution of clothes (P＜.05) more than female students did. 4) In the part of food, there was significant difference between male and female students in the kinds and function of nutritive elements(P＜.05). Parents and students emphasized the importance of selecting good food. 5) In the part of housing, male students emphasized home planning, and arrangements and facilities of house more than female students did. Female students emphasized the interior of the house(P＜.05). Fathers emphasized the planning of a residential space and arrangements and facilities of a house(P＜.05) more than mothers did. 6) In the parts of human development and upbringing, and upbringing, male students emphasized the significance of being parents, family planning and the population problem(P＜.001), pregnancy and delivery(P＜.01), and the chracteristic of juvenile development(P＜.05) more than female students did. Parents and students emphasized the importance of prevention and countermeasures for diseases and the significance of being parents.

• Ecology and Resilient Infrastructure
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• v.2 no.3
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• pp.216-223
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• 2015

The conservation project to protect an endangered fish, Gobiobotia naktongensis was executed against declining the gene diversity of the fish after the construction of Youngju Dam in a sand-bed stream (Naeseongcheon Stream). We tried to move the populations of G. naktongensis from submerged planned sites to alternative habitats, bred artificially and augmented the juveniles to optimal habitat, and monitored the results of the restoration implementation. No entity of G. naktongensis was confirmed at the planned submerged sites despite attempting to capture more than 8 times and eventually the movement to alternative habitat could not be implemented. About 40 individuals of G. naktongensis were captured in the Naeseongcheon Stream and a total of 5,000 individuals were artificially spawned up. The population of juvenile inherited the genetic diversity from the brood stock. The bred juveniles were discharged at the selected optimal site that had a habit condition what was similar to their natural habitat. The micro-dispersion around the discharging area was found at the early stages of the augmentation. The re-capturing rate of discharged juveniles was reduced as time passed. The discharged juveniles seemed to adapt to the natural environment of Naeseongcheon Stream. The observation of their high abdominal distension and excrement demonstrated that the juveniles fed successfully in the discharging area. Therefore, securement of genetic homogeneity and enhancement of restoration population of G. naktongensis in upstream and downstream of Youngju Dam site from the artificial seed producing seemed to be primarily successful and long-term monitoring and analysis of the effect was expected to be necessary.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1383-1391
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• 2016

The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to section Flavi, based on the morphological characteristics and sequence analysis of the ITS rDNA, calmodulin (CaM), and β-tubulin (BenA) genes. The strain reduced the pH value to 5.62 after 7 days of incubation. Organic acid analysis revealed the presence of citric acid (515.0 mg/kg), malic acid (506.6 mg/kg), and fumaric acid (21.7 mg/kg). The three organic acids showed moderate nematicidal activities, but the mixture of citric acid, malic acid, and fumaric acid did not exhibit the full nematicidal activity of the culture filtrate of EML- DML3PNa1. Bioassay-guided fractionation coupled with ¹H- and ¹³C-NMR and EI-MS analyses led to identification of kojic acid as the major nematicidal metabolite. Kojic acid exhibited dose-dependent mortality and inhibited the hatchability of M. incognita, showing EC₅₀ values of 195.2 μg/ml and 238.3 μg/ml, respectively, at 72 h post-exposure. These results suggest that A. oryzae EML-DML3PNa1 and kojic acid have potential as a biological control agent against M. incognita.

• Archives of design research
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• v.19 no.2 s.64
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• pp.293-300
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• 2006

Mr. Miyazaki's heroines tend to be plucky young women who combine guileless decency with tough-mindedness. However, in his the latest film the female character often change herself into wise older women who sometimes serve as foils, sometimes as mother figures. His heroine, Sophie, starts out as a shy girl, but then a curse transforms her into 90-year-old. Her partner is Howl who supports and is supported by Sophie their mutual drawbacks. At first horrified by the change, she comes to embrace it as a liberation from anxiety, fear and self-consciousness, and discovers in herself a new zest for adventure. The castle is surely one of the most extraordinary contraptions to appear on screen recently, more ingenious in its way than the intergalactic armada. It ranges freely from place to place and is also, somehow, in several places at once, its magical front door opening onto different landscapes with the flip of a knob. It is not exactly haunted, but it is nonetheless infused with whimsical and sometimes somber spirits. Sophie, in both her incarnations, joins an impressive sisterhood of Miyazaki heroines, whose version of girl power presents a potent alternative to the mini-machismo that dominates American juvenile entertainment.

• Reproductive and Developmental Biology
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• v.33 no.3
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• pp.163-169
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• 2009

Many biological systems are regulated by an intricate set of feedback loops that oscillate with a circadian rhythm of roughly 24 h. This circadian clock mediates an increase in body temperature, heart rate, blood pressure, and cortisol secretion early in the day. Recent studies have shown changes in the amplitude of the circadian clock in the hearts and livers of streptozotocin (STZ)-treated rats. It is therefore important to examine the relationships between circadian clock genes and growth factors and their effects on diabetic phenomena in animal models as well as in human patients. In this study, we sought to determine whether diurnal variation in organ development and the regulation of metabolism, including growth and development during the juvenile period in rats, exists as a mechanism for anticipating and responding to the environment. Also, we examined the relationship between changes in growth factor expression in the liver and clock-controlled protein synthesis and turnover, which are important in cellular growth. Specifically, we assessed the expression patterns of several clock genes, including Per1, Per2, Clock, Bmal1, Cry1 and Cry2 and growth factors such as insulin-like growth factor (IGF)-1 and -2 and transforming growth factor (TGF)-${\beta}1$ in rats with STZ-induced diabetes. Growth factor and clock gene expression in the liver at 1 week post-induction was clearly increased compared to the level in control rats. In contrast, the expression patterns of the genes were similar to those observed after 5 weeks in the STZ-treated rats. The increase in gene expression is likely a compensatory change in response to the obstruction of insulin function during the initial phase of induction. However, as the period of induction was extended, the expression of the compensatory genes decreased to the control level. This is likely the result of decreased insulin secretion due to the destruction of beta cells in the pancreas by STZ.

• The Korean Journal of Pesticide Science
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• v.11 no.3
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• pp.164-170
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• 2007

The concept of innate immunity in insects which refers to the first line of host defense constitutes the humoral and cellular components which are involved in recognition and actively participate in the elimination of the intruding foreign micro- or macro-organisms. Several recent studies suggest that juvenile hormone (JH) modulates the cellular immune reactions in response to pathogen. In this study, pyriproxyfen (a JH agonist as an insect growth regulator) was tested in its any inhibitory effect on the immune reactions of the beet armyworm, Spodoptera exigua. To this end, five different hemocyte morphotypes of final instar S. exigua were identified by phase contrast microscopy. Plasmatocytes and granular cells, which constitute about 90% of the total hemocyte count, were prominently distinguished based on their basophilic/acidophilic nature using Giemsa stain. The role of pyriproxyfen on the functional ability of hemocytes was analyzed using FITC-labeled Providencia vermicola for the phagocytic potential of the hemocytes. Both granular cells and plasmatocytes exhibited phagocytosis behavior. Pyriproxyfen significantly inhibited the phagocytosis of both cell types, proposing its novel action as an immunosuppressant.