• 한국식품영양과학회지
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• 제21권3호
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• pp.291-295
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• 1992

고들빼기의 부위별 메탄을 엑스가 고콜레스테롤 생쥐의 혈청 콜레스테롤농도에 미치는 영향을 살펴보았다. 고콜레스테롤 생쥐는 식이에 1% 콜레스테롤과 0.5% 답즙산을 첨가하므로서 유도하였으며. 잎의 메탄을 엑스를 투여한 군은 대조군에 비해 혈청 콜레스테롤 농도를 현저히 감소시켰으나 뿌리의 경우에는 효과가 나타나지 않았다. 또한 잎의 메탄을 엑스는 고콜레스테롤 흰쥐에 대해서도 100mg/kg투여 용량에서 혈청 콜레스테롤 농도를 현저히 감소 시켰으며 동맥경화성 지표를 개선시켰다. 반면에, 정상흰쥐에 대해서는 효과가 나타나지 않았다. 이상과 같은 결과로 잎의 메탄을 엑스는 과잉의 콜레스테롤을 섭취하였을때 체내대사 이용도를 증가시키므로서 혈청콜레스테롤 농도를 감소시킨다고 생각된다.

• 한국수산과학회지
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• 제54권5호
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• pp.742-750
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• 2021

Ixeris repens is a type of halophyte that grows in high salinity sands found in coastal sand dunes and sandy shores. This study was conducted to investigate the contents, antioxidant potency, and physiological activities of I. repens. In analyses of general composition, carbohydrate, protein, ash, and moisture content were 57.42%, 10.48%, 11.99% and 10.29%, respectively. Potassium, calcium, sodium, and magnesium were its most prevalent minerals. The solvents used to extract I. repens were 70% ethanol, 80% methanol, and distilled water. Among the resultant extracts, ethanol and methanol extracts displayed higher total polyphenol and flavonoid contents than the water extract. ABTS (IC50, 0.12 mg/mL) and FRAP (0.77 mM) radical scavenging activity were highest in the water extract, while methanol extract exhibited the strongest DPPH radical scavenging activity (IC50, 1.32 mg/mL), NO scavenging activity (IC50, 4.10 mg/mL), and reducing power (EC50, 0.14 mg/mL). Tyrosinase, elastase, and α-glucosidase inhibitory activities were highest in the ethanol extract. The ethanol extract also possessed the most potent acetylcholinesterase inhibitory activity. These results indicate that I. repens may be useful as an antioxidant, and a functional substance in food and pharmaceutical materials.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.308-313
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• 2008

Ixeris dentata (ID, family Asteraceae), called Seumbakuy in Korea, was fermented with lactic acid bacteria (LAB) and their antiallergic activities were investigated. Fermentation of ID with Bifidobacterium breve or Lactobacillus acidophilus increased its inhibition of degranulation in RBL-2H3 cells induced by the IgE-antigen complex. Oral administration of these extracts to mice inhibited the passive cutaneous anaphylaxis (PCA) reaction induced by the IgE-antigen complex and scratching behaviors induced by compound 48/80. The fermented ID more potently inhibited the PCA reaction and scratching behaviors than the non-fermented one. These extracts also inhibited mRNA expression of TNF-${\alpha}$ and IL-4, as well as NF-${\kappa}B$ activation in RBL-2H3 cells induced by the IgE-antigen complex. These findings suggest that LAB fermentation improves ID-mediated inhibition of IgE-induced allergic diseases such as rhinitis and asthma, and that ID works by inhibiting degranulation and NF-${\kappa}B$ activation in mast cells and basophils.

• 한국식품영양과학회지
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• 제27권3호
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• pp.525-530
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• 1998

The effects of godulbaegi(Ixeris sonchifolia H.) powder on body weight gain, lipid and protein concentrations, and enzyme activities were studied in growing male rats for 4 and 8 week. The fats (Sprague-Dawley) were fed diets containing 5% godulbaegi or without. The body weght gain, food efficiency ratio, protein efficiency ratio, hemoglobin concentration and hematocrit value of rats fed godulbaegi diet were similar to the control diet. The ratio of spleen weight to body weight in godulbaegi group for 8 weeks was greater than in that of control group, but they were similar both feeding groups in liver and kidney. The concentrations of the total lipid and triglyceride in serum were increased in rats fed godulbaegi diet for 4 week than in those fed control diet, but those were decreased in rats fed godulbaegi diet for 8 week than in those fed the control diet. The concentrations of the phospholipid, total cholestrol and HDL-cholestrol in serum were decreased in rats fed godulbaegi diet for 8 week than in those fed control diet. The HDL-cholestrol/total cholestrol ratio and athergenic index were similar to both diet groups. There were no differences in growth, serum total protein, albumin concentrations, aspartate aminotransferase and alanine aminotransferase activities between rats fed godulbaegi diet and those fed the control diet.

• Archives of Pharmacal Research
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• 제25권3호
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• pp.289-292
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• 2002

The ethyl acetate extract of the leaves of Ixeris sonchifolia afforded two new and two known sesquiterpene lactone glucosides of the guaiane-type, together with a known alkenol glucoside. The known compounds were identified as ixerin Z (1), ixerin Z-6'-p-hydroxyphenylace-tate (2), and (Z)-3-hexen-1-ol-$\beta$-D-glucopyranoside (3), respectively. The structures of the new compounds were elucidated as 11, 13a-dihydroixerin Z [4, 3-hydroxy-2-oxo-guaia-1 (10), $3-dien-5{\alpha},6{\beta},7{\alpha},11{\beta}H-12,6-olide-3-O-{\beta}-D-glucopyranoside],{\;}and{\;}3,10{\$beta}-dihydroxy-2-oxo-guaia-3,11(13)-dien-1{\alpha},5{\alpha},6{\alpha},7aH-12,6-olide-10-O-{\beta}-D-glucopyranoside$ (5), respectively. The cytotoxicity of these compounds against human hepatocellular carcinoma cell (HepG2) and human melanoma cell (SK-MEL-2) was evaluated.

• 생명과학회지
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• 제8권2호
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• pp.131-136
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• 1998

외근에 귀화한 가시상추(Lactuca serriola)의 분산과 적응을 연구하였다. 가시ㅛ　추의 분포는 한반도 중부지방의 남부에 제한되었다. 그 분포지역은 인간에 의한 이동으로 점차 확산되고 잇었다. 10개의 조사지역의 관측결과 천이의 초기단뎨에 있는 노변식생으로 나타났다. 즉 측수의 90%의 초본층과 5%의 관목층으로 구성되었으나 교목층은 없었다. 우점종군은 Kummeroverowia stipulacea, Ixeris chimensis, Ambrosia artemisiifolia., Lactuca indica, Tiungia sonchifolia, Cephalonoplos segetum, Rubus parvifolius, Ixeris polycephala, Hemistepta Lyrata, Cercis chinensis Artemisia capillaris 이었다. 조사지역은 식생의 높은 상이도로 4개유형으로 구분되지만 많은 우형이 나타나는 것은 아직 가시상추의 특유한 군락은 형성하지 못하고 있음을 보였다.

• Archives of Pharmacal Research
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• 제26권2호
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• pp.151-156
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• 2003

We investigated the anti-proliferative effects of luteolin and apigenin, isolated from Ixeris sonchifolia Hance, on HepG2 human hepatocellular carcinoma cells. In MTT assay luteolin showed more efficient anti-proliferative effects on cells than apigenin did. According to propidium iodide staining and flow cytometry studies, we postulated that these effects might be a result of cell cycle arrest. Hence we examined the changes of protein expressions related to cell cycle arrest. Western blotting data demonstrated that the down-regulated expression of CDK4 was correlated to the increase of p53 and CDK inhibitor $p21^{WAF1/CIP1}$ protein. These data suggest that luteolin may have potential as an anti-cancer agent.

• 한국식품영양과학회지
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• 제24권2호
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• pp.305-312
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• 1995

Ixeris dentata was extracted with methanol and then the methanol extract was further fractionated to hexane, chloroform, ethyl acetate, butanol and aqueous fraction. The methanol extract of lxeris dentata had the strong antimutagenic effect on the aflatoxin B1(AFB1) and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Ames mutagenicity test and SOS chromotest. Among the solvent extracted fractions from the methanol extract, the chloroform fraction exhibited the greatest antimutagenic effect suppressing the mutagenicity of AFB1 with inhibition rate of 74 percent. The methanol extract of Ixeris dentata also revealed the inhibitory effect on the growth of MG-63 human osteosarcoma cells after 6 days of breeding at 37℃. The chloroform fraction and the ethyl acetate fraction from the methanol extract of lxeris dentata were most effective and inhibited the growth of MG-63 cells by 97 and 93 percent, respectively. It is suggested that the inhibitory effects of lxeris dentata on the mutagenicity and the growth of MG-63 human osteosarcoma cells are strong in the lipid soluble fractions.

• 동아시아식생활학회지
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• 제24권6호
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• pp.739-747
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• 2014

본 연구는 인체유방암세포인 MDA-MB-231세포를 이용하여 IDE를 처리하였을 때 세포사멸에 미치는 영향을 세포화학적 방법으로 확인하였다. IDE를 각각 0, 20, 30 및 $40{\mu}g/mL$ 첨가하여 24시간 배양한 후, 세포증식 억제, 막투과성, 세포내 ROS 분석 및 세포사멸 단계의 특성을 FACS 분석하고, RT-PCR에 의한 사멸관련 유전자 중 Bax/Bcl-2 ratio 분석을 통하여 IDE의 항암작용의 조절작용을 밝히고자 하였다. MTT의 세포 증식 억제는 첨가된 IDE의 농도에 따라 유의적으로 감소하였다(p<0.05). 이와 동시에, trypan blue에 대한 염색성과 DCF-DA 형광 분석의 결과는 각각, 막투과성과 세포내 ROS 농도가 모두 농도 의존적으로 증가됨을 보였다(p<0.05). 이와 같은 IDE 농도에 따른 세포화학적 변화 중에서 세포의 초기사멸에서 후기사멸 과정으로 급격한 사멸단계의 변화가 특히, IDE 농도 30과 $40{\mu}g/mL$에서 일어났다. RT-PCR 분석에 의한 Bax/Bcl-2 ratio도 IDE 농도 30과 $40{\mu}g/mL$에서 급격히 증가하였다(p<0.05). 이와 같은 세포화학적 결과와 RT-PCR 결과를 종합해 볼 때, IDE의 유방암세포(MDA-MB-231)에 대한 세포사멸작용은 막 투과성의 증가와 ROS 증가를 통하여 세포에 점진적인 손상을 일으키며, 이는 세포의 생화학적 변화도 초래하여 세포 증식 억제를 감소시켜, 결국 세포내의 사멸관련 유전자 지표인 Bax/Bcl-2 ratio를 크게 변화시키는 일련의 세포사멸을 점진적으로 유도시켜 항유방암의 효과의 가능성을 제시하였다.

• 한국식품저장유통학회지
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• 제15권4호
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• pp.524-531
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• 2008

씀바귀뿌리를 이용하여 침출차를 제조하고자 건조온도 및 처리조건을 달리하여 유리당, 유기산, cynaroside 및 총폴리페놀성물질 함량, 유리아미노산, 색도 및 관능적 특성을 검토하였다. 총유리당, 유기산, 유리아미노산 및 cynaroside 의 함량은 천일건조한 것이 가장 높았으며 총폴리페놀성 함량은 열풍건조 후 볶음 처리한 것이 가장 높았다. 건조한 씀바귀뿌리의 유리당 중 sucrose, glucose, fructose 및 galactose는 열풍건조에 의하여 감소하였고 천일건조 시 검출되지 않았던 maltose가 열풍건조에 정량되었으며 증숙 후 열풍건조 시 1.67%의 높은 함량을 보여주었다. 씀바귀뿌리 내에 함유되어있는 유리아미노산함량 중 arginine이 669.2 mg%으로 가장 높았고 뒤이어 serine이 379.9 mg%으로 높은 함량을 보여주었다. 씀바귀뿌리 건조분말의 색도는 열풍건조 후 볶음처리를 한 것이 밝기, 적색도 및 황색도 모두 가장 높았으나 열수침출 후의 색도는 적색도와 황색도는 타처리구에 비하여 가장 높았지만 밝기는 가장 낮게 나타났다. 씀바귀뿌리를 이용한 침출차의 관능검사결과 열풍건조 후 볶음처리한 처리구가 가장 높은 점수를 보였다.