• Bulletin of the Korean Chemical Society
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• v.28 no.10
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• pp.1787-1791
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• 2007

Several hydroxylated cinnamic acid derivatives were prepared from the corresponding acids and amino acid residues, and their hypocholesterolemic activities were evaluated in high cholesterol-fed mice. The presence of the double bond in hydroxylated cinnamide derivatives decreases cholesterol-lowering activities and the number of free phenolic hydroxy groups affect greatly the activities. 3,4-Dihydroxy hydrocinnamides obtained from amino acid derivatives containing a hydrophobic side chain such as alanine, valine, phenylalanine, and isoleucine exhibited potent cholesterol-lowering activities.

• YAKHAK HOEJI
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• v.32 no.3
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• pp.170-176
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• 1988

The resolution of five amino acids derivatives by gas chromatography on phenylthiohydantoin derivatives as chiral stastionary phase was investigated. (S)-5-isopropyl, isobutyl, (sec)-butyl and benzoyl derivatives of $N^3-phenyl-2-thiohydantoin$ were synthesized from L-valine, L-leucine, L-isoleucine and L-phenylalanine, respectively. The phases were employed at several column temperatures ($140^{\circ}C{\sim}210^{\circ}C$). The separation factors were compared with each stationary phase with varying R groups. The possible mechanism of chiral recognition was investigated by NMR technique.

• YAKHAK HOEJI
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• v.10 no.2_3
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• pp.15-19
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• 1966

In order to find 2-(5-nitro)furylacrylic acid derivatives possessing antimicrobial activity, nine new 2-(5-nitro)furylacrylamino acids were synthesized which were obtained by the action of 2-(5-nitro)furylacryla chloride on amino acids, such as $_{L}$-phenylalanine, glycine, $_{L}$-isoleucine, $_{L}$-glutamic acid, $_{DL}$-methionine, $_{L}$-threonine, $_{L}$-valine, $_{L}$-tryptophan and $_{DL}$-alanine, according to Schotten-Baumann method. These compounds generally showed a good bactericidal and bacteriostatic activity against Bacillus subtilis but were less effective against staphylococcus aureus, Proteus vulgaris and Escherichia coli. Of the above nine compounds, 2-(5-nitro)furylacryl glycine exhibited a good bactericidal activity.

• Journal of Nutrition and Health
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• v.7 no.4
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• pp.12-20
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• 1974

Quantitative analysis was achieved by gas-liquid chromatographic method (GLC) with a single column system of OV-17 for 16 of protein amino acids in mushroom (agaricus bisporus). The quantities of protein amino acids in mushroom were determined $48.32{\sim}255.94mg%$ alanine, $108.6F{\sim}364.82mg%$ glycine, $124.30{\sim}314.17mg%$ Valine, $32.99{\sim}418.79mg%$ leucine and isoleucine, $151.78{\sim}669.07mg%$ threonine, $88.12{\sim}4F6.3Fmg%$ Serine, $21.9F{\sim}114.94mg%$ Hydroxyproline, $20.F4{\sim}174.63mg%$ proline, $34.52{\sim}173.59mg%$ Methionine, $225.25{\sim}1417.61mg%$ Aspartic Acid, $10F.00{\sim}392.17mg%$ Phenylalanine, $12F.46{\sim}535.65mg%$ Glutamic Acid and Lysine, Tyrosine in trace amount.

• Journal of the Korean Society of Food Science and Nutrition
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• v.3 no.1
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• pp.53-68
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• 1974

The thiohydantoin derivative derived from amino acid was used for the stepwise sequence analysis of peptide or protein from the carboxyl termini. Recently, SUZUKI reported the mass spectrometric identification about a part of these compounds. In this paper, was described the mass spectrometric identification of thiohydantoins derived from 20 amino acids. Mass spectra were obtained with a mass spectrometer, JEOL model JMS-06H and samples were introduced with a direct inlet probe. The molecular ion peaks and fragment ion peaks were identified easily, because these peaks appeared differently every amino acids and specially, it was easy discrimination between leucine and isoleucine. It is suggested that mass spectrometry was one of the useful methods to identify thiohydantoins derived from amino acids.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.35-39
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• 1991

Properties of a protease purified from Neungee[Sarcodon aspratus(Berk, ) S. Ito] have been investigated. The enzyme displays a glycosylated serine protease. The enzyme is able to hydrolyze alanine glycine methionine glutamine and cysteine of N-CBZ and N-t-BOC-L-amino acid derivatibes relatively strongly but splits valine proline and isoleucine derivatives with low affinity which means the enzyme has the broad substrate spectrum toward the amino acids. Interestingly the enzyme was inhibited by bromelain inhibitor. That is the active site environ-ment of the enzyme is believed to be similar to that of bromelain However peptide mapping studies show that the two enzymes have distinct different cleavage sites.

• BMB Reports
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• v.31 no.3
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• pp.258-263
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• 1998

Acetolactate synthase (ALS) is the common enzyme in the biosynthetic pathways leading to leucine, valine, and isoleucine. Tobacco ALS was expressed in E. coli and purified to homogeneity. The recombinant tobacco ALS was inactivated by thiol-specific reagents, N-ethylmaleimide (NEM) and 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Inactivation of the ALS by NEM followed pseudo-first order kinetics and was first order with respect to the modifier. The substrate pyruvate protected the enzyme against the inactivation by NEM and DTNB. Extrapolation to complete inactivation of the enzyme by DTNB showed modification of approximately 2 out of 4 total cysteinyl residues (or 2 cysteinyl and 1 cysteinyl residues), with approximately 1 residue protected by pyruvate. The tobacco ALS was also inactivated by the tryptophanspecific reagent, N-bromosuccinimide (NBS), and was similarly protected by pyruvate. The kinetics of the inactivation was first-order with respect to NBS. The present data suggest that cysteinyl and tryptophanyl residues play a key role in the catalytic function of the enzyme.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.96.3-96
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• 1978

Biotin 요구성 glutamic acid 생산능을 가진 Br-evibacterium sp.의 isoleucine 영양요구성 변이주가 다량의 L-leucine을 생성함을 발견하였다. 본균주에 관한 탄소원, 질소원, 무기염, vitamin, amino acid 등의 영양물질과 온도, 공기주입량, pH등에 관한 실험으로 다음과 같은 결과를 얻었다. 본균주는 탄소원, 질소원으로서 10% glucose와 4% $(NH_4)_2SO_4를$ 공합하여 $30^{\circ}C,$ 88시간 배양시킨 결과, 액중에 21 mg/ml의 L-leucine이 축적되었다. Fe$^{2+}$와 $Mn^{2+}$의 동시첨가는 L-leucine생성에 효과적이었으며 thiamine 역시 L-leucine 생성에 효과적이었다. 30ι jar fermentor 에서 폐당밀을 주원료로 한 실험결과, 폐당밀, $(NH_4)/SO_4,$ $KH_2PO_4,$ $MgSO_4ㆍ7H_2O로$ 구성된 액체배지에서 $30^{\circ}C,$ 36시간 배양시킨 것이, 27mg/ml의 L-leucine이 축적되었으며 이때의 대당수율은 16%이었다.

• Korean Journal of Pharmacognosy
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• v.16 no.4
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• pp.206-209
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• 1985

White needle crystalline compound, mp.\;78{\sim}79$, was isolated from the petroleum ether fraction of the aerial parts of Chenopodium acuminatum. This compound was supposed to be cycloalkane by chemical properties, spectral data and elemental analysis. After extraction with petroleum ether, the methanolic extract of this plant found to contain aspartic acid, threonine, serine, glutamic acid, glycine, cystine, valine, isoleucine, tyrosine, and phenylalanine. Inorganic elements contained in this plant are as following: Mg 4.84%, Ca 9.602%, Zn 1.11%, K 29.104%, Mn 5.124%, Fe 27.039%, Co 0.31%, Pb 0.995%, Na 0.039% and Cu, 0.0076%.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.204-204
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• 1994

Ganoderma lucidum의 균사체로 부터 얻어진 G009의 이화학적 특성을 규명하였다. G009는 넓은 범위의 분자량 분포를 가지고 있는 다당체로 분자량 9.4kD의 물질이 주성분이었다 G009의 당의 함량은 약 70%이었고 단백질은 약 2.4%인 것으로 나타났다. G009의 주요 구성당은 glucose, xylose, mannose, galactose 등이었고, glycine, leucine, alanine, valine, isoleucine, proline 등 17종의 아미노산이 확인되었다. 유기원소 분석 결과 G009는 탄소 약 40%, 수소 5.7%, 질소 1.8%의 구성비를 보였으며, 무기원소 분석결과 Ca, Mg, Zn 등이 확인되었다. 이상의 결과로 부터 G009는 9.4 kD의 분자량을 갖는 단백다당체를 주성분으로 하는 당화합물로 추정된다.