• Title/Summary/Keyword: Isolation system

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Isolation of Polysaccharides Modulating Intestinal Immune System and Single Oral Dose Toxicity Test in Astragalus membranaceus Abovegroud Parts (황기 지상부로부터 장관면역 활성 다당체의 분리 및 단회 경구 투여 독성시험)

  • Choi, Ri Na;Park, Yeong Chul;Lee, Ji Sun;Kim, Jung Woo;Kim, Jong Bong;Cheoi, Yu Soon;Kim, Kwang Ki;Lee, Jae Geun;Yu, Chang Yeon;Kim, Seung Hyn;Chung, Ill Min;Kim, Jae Kwang;Lim, Jung Dae
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.4
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    • pp.276-288
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    • 2014
  • The six polysaccharide fractions were prepared by chromatographic procedure from the hot water extracts of the aboveground parts of Astragalus membranaceus. These six polysaccharides from aboveground parts of Astragalus membranaceus Bunge were tested for gut-mucosal immune activity and acute toxicity. In a view of molecular weight, the six fractions were estimated to be 75000, 88000, 129000 and 345000 Da, respectively. Component sugar analysis indicated that these fractions were mainly consisted of galactose (46.3 ~ 11.8%) and arabinose (35.4 ~ 9.9%) in addition to glucose, rhamnose, fucose, arabinose, xylose, mannose, glucuronic acid and galacturonic acid. Among the six major purified polysaccharides, AMA-1-b-PS2 showed highest bone merrow cell proliferation and lymphocyte of Peyer's patch stimulating activity. It may be concluded that intestinal immune system modulating activity of aboveground parts from Astragalus membranaceus Bunge is caused by polysaccharides having a polygalacturonan moiety with neutral sugars such as arabinose and galactose. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs. The results indicated that lethal dose 50 ($LD_{50}$) of water extracts from Astragalus membranaceus-aboveground parts was found to be higher than 5000 mg/kg/day in this experiment. From the above results, we may suggest that Astragalus membranaceus-aboveground parts might have useful as a safe material for functional food and pharmaceutics.

Isolation and Characteristics of Exopolysaccharide Producing Bacteria in a Ginseng Root System (인삼 근계로부터 다당 생성세균의 분리 및 특성)

  • Cho, Geon-Yeong;Jeon, In-Hwa;Han, Song-Ih;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.49 no.3
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    • pp.297-300
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    • 2013
  • EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed $9.0{\times}10^6$ CFU/g, $7.0{\times}10^6$ CFU/g, and $1.4{\times}10^3$ CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6 strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter (6 strains), Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain). Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1 (KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were detected. The major EPS sugar of these strains was glucose (72.7-84.9%).

The Partial Full Duplex Relay Scheme for Cell ID Detection of Type 1 Relay in 3GPP LTE-Advanced System (3GPP LTE-Advanced 시스템에서 Type 1 relay의 셀 ID 검출을 위한 부분 전이중 relay 기법)

  • Min, Young-Il;Jang, Jun-Hee;Choi, Hyung-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.6A
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    • pp.558-567
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    • 2011
  • In this paper, we propose a partial full duplex relay scheme for 3GPP (3rd Generation Partnership Project) LTE (Long Term Evolution)-Advanced system using a Type 1 relay. The Type 1 relay as inband relay is prohibited to transmit and receive simultaneously because of self-interference. Therefore, the Type 1 relay cannot receive synchronization signals which are transmitted to eNB. To overcoming this problem, we propose the partial full duplex relay scheme which transmits to R-UE (Relay-User Equipment) and receives from eNB (evolved NodeB) simultaneously when eNB and the Type 1 relay transmit subframes which have synchronization signals. Additionally, for solving self-interference, the Type 1 relay transmitter and receiver antennas are sufficiently sufficiently isolated and self-interference cancellation is applied for the self-interference signal from the relay transmitter. Thus, the partial full duplex relay scheme can receive synchronization signals from eNB and solve the problems of conventional solutions and we propose the partial channel estimation scheme for partial full duplex relay scheme using SCI. By extensive computer simulation, we verify that the partial full duplex relay scheme is attractive and suitable for the Type 1 relay system.

Isolation and Characterization of An Alcohol Fermentation Strain from Anaerobic Acid Fermentor to Treat Food Wastes (음식폐기물 처리용 혐기성 산 발효조로부터 알코올발효 균주의 분리 및 특성)

  • Kim, Jung-Kon;Han, Gui-Hwan;Yoo, Jin-Cheol;Seong, Chi-Nam;Kim, Seong-Jun;Kim, Si-Wouk
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.451-455
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    • 2006
  • An efficient pilot scale (10 ton) three-stage methane fermentation system to digest food waste has been developed in this laboratory. This system consisted of three stages: semianaerobic hydrolysis, anaerobic acidogenesis and strictly anaerobic methanogenesis. From the secondary acidogenesis reactor, a novel strain KA4 responsible for alcohol fermentation was isolated and characterized. The cell was oval and its dimension was $5.5-6.5{\times}3.5-4.5\;{\mu}m$. This strain was identified as Saccharomyces cerevisiae KA4 by 26S rDNA D1/D2 rDNA sequence. Optimal culture temperature was $30-35^{\circ}C$. Cells were tolerant to 5% (v/v) ethanol concentration, however, were inhibited significantly by higher ethanol concentration up to 7%. The strain could grow well up to 50% (w/v) initial glucose concentration in the YM liquid medium, however, optimal concentration for ethanol fermentation was 10%. It could produce ethanol in a broad initial pH range from 4 to 10, and optimal pH was 6. In this condition, the strain converted 10% glucose to 7.4% ethanol during 24 hr, and ethanol yield was estimated to be 2.87 moi EtOH/mol glucose.

A Survey on Diseases and Symptoms of the nurses who were Employed at the City and University hospitals in Seoul (서울시내 종합병원에 근무하는 간호원들의 질병 및 증상에 관한 조사)

  • 김매자;이선자;박순자
    • Journal of Korean Academy of Nursing
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    • v.5 no.1
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    • pp.70-78
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    • 1975
  • The investigators conducted this survey to find out prevalence of diseases and symptoms of the 812 nurses who were working at the 12 Seoul city and University's hospital in Seoul by retrospective study The results of survey were summarized as follows: 1. Distribution of Prevalence Rate of Diseases and Symptoms. 1) An average prevalence rate of diseases and Symptoms of the nurses/100 persons was 64.3 cases at Present October I.1972 2) The total number of diseases and Symptoms was 522 cases at present Oct, I 1972 for the survey nurses. The prevalence rate of following diseases/100 persons were as, foot sore-11, 3. myopia-9.7, neuralgia-9.6, anemia-9.1, insomnia-8.6 cases and the corrected number percent of prevalence rate of diseases and symptoms of the above diseases into 100 denominator were as follows : foot sore-17.6% , myopia-15.4%, neuralgia-14.9%, anemia-14.2% insomnia-13.2%. 3) The prevalence rate of diseases and symptoms/100 person for the age group of 45 and over was 140.0 cases and the age of 35-39 years was 27.3 cases. 4) The prevalence rate of diseases and symptoms/100 persons by the rotation system of nurses'duty/day were as follows: two shift system rotation-86.7 cases, three shift system rotation-67.9 cases and day duty only was 56.2 cases, 5) The prevalence rate of diseases and symptoms/100 person by the basic nursing education background seemed to be lower occurrence against to the higher education and it was 94.4 cases at the Technical Nursing High School graduates. 6) The prevalence rate of diseases and symptoms/100 persons by the marital status of the nurses were as follows; single-64.8 cases, married-48, 7 cases, and widowed-28.6 cases. II. Relationship Between working Experience and prevalence of the Disease and symptoms. 1) There were no relationship by statistical test between prevalence of eye disease and experience at the eye ward(p〉0.05), skin disease and experience at the dermatology ward (p〉0.05), foot sore and experience at the operating room (p〉0.05), varicose vein and experience at the operating room (p〉0.05), sore finger and experience at the central supply room (p〉0.05), infectious disease and experience at the isolation ward(p〉0.05). 2) There was significant relationship by statistical test between pulmonary tuberculosis and experience at the tuberculosis ward (p〉0.05) prevalence of pulmonary tuberculosis was five times in experienced group than non experienced group.

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A Study on the Mobile Communication System for the Ultra High Speed Communication Network (초고속 정보통신망을 위한 이동수신 시스템에 관한 연구)

  • Kim, Kab-Ki;Moon, Myung-Ho;Shin, Dong-Hun;Lee, Jong-Arc
    • Journal of IKEEE
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    • v.2 no.1 s.2
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    • pp.1-14
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    • 1998
  • In this paper, Antenna, LNA, Mixer, VCO, and Modulation/Demodulation in Baseband processor which are the RF main components in Wireless LAN system for ultra high-speed communications network are studied. Antenna bandwidth and selective fading due to multipath can be major obstacles in high speed digital communications. To solve this problem, wide band MSA which has loop-structure magnetic antenna characteristics is designed. Distributed mixer using dual-gate GaAs MESFET can achieve over 10dB LO/RF isolation without hybrid, and minimize circuit size. As linear mixing signal is produced, distortions can be decreased at baseband signals. Conversion gain is achieved by mixing and amplification simultaneously. Mixer is designed to have wide band characteristics using distributed amplifier. In VCO design, Oscillator design method by large signal analysis is used to produce stable signal. Modulation/Demodulation system in baseband processor, DS/SS technique which is robust against noise and interference is used to eliminate the effect of multipath propagation. DQPSK modulation technique with M-sequences for wideband PN spreading signals is adopted because of BER characteristic and high speed digital signal transmission.

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The Design of an Auto Tuning PI Controller using a Parameter Estimation Method for the Linear BLDC Motor (선형 추진 BLDC 모터에 대한 파라미터 추정 기법을 이용하는 오토 튜닝(Auto Tuning) PI 제어기 설계)

  • Cha Young-Bum;Song Do-Ho;Koo Bon-Min;Park Moo-Yurl;Kim Jin-Ae;Choi Jung-Keyng
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.4
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    • pp.659-666
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    • 2006
  • Servo-motors are used as key components of automated system by performing precise motion control as accurate positioning and accurate speed regulation in response to the commands from computers and sensors. Especially, the linear brushless servo-motors have numerous advantages over the rotary servo motors which have connection with the friction induced transfer mechanism such as ball screws, timing belts, rack/pinion. This paper proposes an estimation method of unknown motor system parameters using the informations from the sinusoidal driving type linear brushless DC motor dynamics and outputs. The estimated parameters can be used to tune the controller gain and a disturbance observer. In order to meet this purpose high performance Digital Signal Processor, TMS320F240, designed originally for implementation of a Field Oriented Control(FOC) technology is adopted as a controller of the liner BLDC servo motor. Having A/D converters, PWM generators, rich I/O port internally, this servo motor application specific DSP play an important role in servo motor controller. This linear BLDC servo motor system also contains IPM(Intelligent Power Module) driver and hail sensor type current sensor module, photocoupler module for isolation of gate signals and fault signals.

Estimate of Nitrous Oxide Emission Factors from Municipal Wastewater Treatment Plants (하수처리 공정별 아산화질소(N$_2$O) 배출계수 산정)

  • Yang, Hyung-Jae;Park, Jung-Min;Kim, Min-Jung
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.12
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    • pp.1281-1286
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    • 2008
  • Nitrous oxide(N$_2$O) is well known as a greenhouse gas that contributes to the global warming (310 times more per molecule than carbon dioxide) and to the destruction of the ozone layer. The objective of this study is to estimate N$_2$O emission factor using an emission isolation flux chamber from municipal wastewater treatment plants. N$_2$O gas was analysed by GC/ECD with 6 port gas sampling valve. The results of this study were as follows. N$_2$O emission factor of 5-Stage process from Y wastewater treatment plants was lowest as 0.94 g-N$_2$O/kg-TN. And that of other processes were 2.65 g-N$_2$O/kg-TN for Activated sludge process, 9.30 g-N$_2$O/kg-TN for Denipho process, and 26.73 g-N$_2$O/kg-TN for Sequencing Batch Reactor process. We have known that 5-Stage process is most appropriate process to reduce greenhouse for municipal wastewater treatment plants.

Comparison of biological activities of essential oils from Foeniculum vulgare Mill, Boswellia carteii Birew and Juniperus rigida Sieb. by a supercritical fluid extraction system (초임계 추출 공법을 이용해 회향, 유향 및 노간주나무로부터 분리한 정유 성분의 생리활성 비교)

  • Lee, Hyun-Soo;Mun, Chul-Hyung;Park, Jin-Hong;Kim, Dae-Ho;Yoo, Jae-Eun;Park, Young-Sik;Ryu, Lee-Ha;Choi, Keun-Pyo;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.115-121
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    • 2003
  • Essential oils from Fennel fruit(Foeniculum vulgare Mill), Olibanum resin(Boswellia carteii Birew) and Needl Juniperus stem(Juniperus rigida Sieb.) were extracted by a supercritical fluid extraction system(SFE) and biological activity of each essential oils were observed. SFE technique was applied for the isolation and purification of nonpolar biologically active essential oils from each samples. The quantitative analysis of essential oils was carried out by gas chromatography-mass spectrometer(GC/MS). About 60% of the growth of AGS and A549 cells were inhibited by adding 1.0g/l of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell(HEL299) was scored as $15{\sim}18%$ for the crude essential oils and 12% for control, respectively. It meant that the essential oils were more effective than the control in anti-mutagenecity tested by CHO V79 cells. The effect of the essential oils on the growth of nerve cells, PC12 was observed as follows: The viable cell density was about two times higher than control.

A Distributed Real-Time Concurrency Control Scheme using Transaction the Rise of Priority (트랜잭션 우선 순위 상승을 이용한 분산 실시간 병행수행제어 기법)

  • Lee, Jong-Sul;Shin, Jae-Ryong;Cho, Ki-Hyung;Yoo, Jae-Soo
    • Journal of KIISE:Databases
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    • v.28 no.3
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    • pp.484-493
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    • 2001
  • As real-time database systems are extended to the distributed computing environment, the need to apply the existing real-time concurrency control schemes to the distributed computing environment has been made. In this paper we propose an efficient concurrency control scheme for distributed real-time database system. Our proposed scheme guarantees a transaction to commit at its maximum, reduces the restart of a transaction that is on the prepared commit phase, and minimizes the time of the lock holding. This is because it raises the priority of the transaction that is on the prepared commit phase in the distributed real-time computing environment. In addition, it reduces the waiting time of a transaction that owns borrowed data and improves the performance of the system, as a result of lending the data that the transaction with the raised priority holds. We compare the proposed scheme with DO2PL_PA(Distributed Optimistic Two-Phase Locking) and MIRROR(Managing Isolation in Replicated Real-time Object Repositories) protocol in terms of the arrival rate of transactions, the size of transactions, the write probability of transactions, and the replication degree of data in a firm-deadline real-time database system based on two-phase commit protocol. It is shown through the performance evaluation that our scheme outperforms the existing schemes.

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