• Korean Journal of Veterinary Service
• /
• v.37 no.1
• /
• pp.45-49
• /
• 2014

Pullorum disease and fowl typhoid are septicemic avian diseases transmitting through egg by transovarian infections. A series of tests has been performing in breeding flocks of chickens and test plans for proper inspection have been modified by government veterinary institute to control of such diseases. To improve inspection plans, different test methods were compared using fowl typhoid positive samples from a poultry farm located in Jeonbuk state in 2012. Based on first inspection, 11 samples among total 200 samples were positive by rapid slide agglutination (RSA) test and 7 samples among RSA positive samples were finally diagnosed as Salmonella Gallinarum infection by ELISA, bacterial isolation, PCR, and histopathologic examination. In the second inspection, 20 samples among total 100 samples were positive by RSA test. Among RSA positive ones, 19 samples were positive by ELISA, S. Gallinarm were successfully isolated in 3 samples, and 16 samples were positive by PCR in the cecal tonsils where were not successful for bacterial isolation. Based on histopathologic examination, severe inflammation in the 13 cecal tonsils and infiltration of lymphocytes and heterophils in the 11 livers were observed. Therefore, we suggest that bacterial isolation, PCR, and histopathologic examination methods in the third inspection need to be further used in various tissues for correct diagnosis and for final eradication of pullorum disease and fowl typhoid in breeding flocks of chickens.

• Journal of Genetic Medicine
• /
• v.17 no.2
• /
• pp.68-72
• /
• 2020

Purpose: Trisomy 21, the cause of Down syndrome (DS) with various medical problems, is the most common aneuploidy during the fetal period. For diagnosis, a non-invasive screening test using maternal blood, which cannot be confirmed and invasive confirmation test with a risk of miscarriage, may be performed. The trophoblast retrieval and isolation of the cervix (TRIC) have been proposed by some researchers as an alternative to overcome the limitations of current tests. We experimented using TRIC to identify the possibility of trisomy 21 for the first time in Asia. Materials and Methods: Three cases of DS were analyzed confirmed by invasive tests (chorionic villus sampling, amniocentesis). All samples of trophoblasts immediately were immersed in phosphate-buffered saline and processed with formalin for fixation. The trophoblasts were isolated using an anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. β-human chorionic gonadotropin (hCG)-expressing cells were considered as trophoblast cells, and the detection rate calculated. DS was confirmed by fluorescence in situ hybridization (FISH). Results: The mean trophoblast detection rate using β-hCG was 78.1%, and the detection rate using FISH was 22.2%. In all cases, the trisomy of chromosome 21 was identified. Conclusion: Trophoblast can be obtained from the five weeks of gestation and has a high detection rate, so it is noted that it can replace the current prenatal genetic test. To realize the clinical application as a prenatal genetic test, we will need additional efforts to identify trisomy 21 as well as other chromosomal abnormalities in future large-scale studies.

• Journal of the Korean Geotechnical Society
• /
• v.34 no.1
• /
• pp.5-16
• /
• 2018

In this study, seismic performance of geotechnical seismic isolation system capable of primary seismic isolation in the ground was evaluated. 1-G shaking table test was used to assess the performance of Teflon or steel slag as geotechnical seismic isolation systems installed beneath superstructure foundation. Response acceleration and response spectra were analyzed considering different input motions. The results were compared with those of fixed foundation structure without seismic isolation system. The steel slag-type seismic isolation system showed significant reduction in acceleration. The teflon-type seismic isolation system did not show significant effects on acceleration reduction in low-to-moderate seismicity condition, but it did show better effects in case of strong seismic condition. As input motion was transferred to the upper mass, the response spectrum of the fixed foundation structure was amplified in the short period range. In contrast, the response spectrum of the structure with seismic isolation using teflon or steel slag amplified in the long period range. It is found that the change of periodicity and the friction characteristics between isolation materials and foundations affected acceleration reduction.

• Advances in environmental research
• /
• v.9 no.3
• /
• pp.215-232
• /
• 2020

Crude oils are essential source of energy. It is majorly found in geographical locations beneath the earth's surface and crude oil is the main factor for the economic developments in the world. Natural crude oil contains unrefined petroleum composed of hydrocarbons of various molecular weights and it contains other organic materials like aromatic compounds, sulphur compounds, and many other organic compounds. These hydrocarbons are rapidly getting degraded by biosurfactant producing microorganisms. The present study deals with the isolation, purification, and characterization of biosurfactant producing microorganism from oil-contaminated soil. The ability of the microorganism producing biosurfactant was investigated by well diffusion method, drop collapse test, emulsification test, oil displacement activity, and blue agar plate method. The isolate obtained from the oil contaminated soil was identified as Bacillus subtilis. The identification was done by microscopic examinations and further characterization was done by Biochemical tests and 16SrRNA gene sequencing. Purification of the biosurfactant was performed by simple liquid-liquid extraction, and characterization of extracted biosurfactants was done using Fourier transform infrared spectroscopy (FTIR). The degradation of crude oil upon treatment with the partially purified biosurfactant was analyzed by FTIR spectroscopy and Gas-chromatography mass spectroscopy (GC-MS).

• Transactions of the Korean hydrogen and new energy society
• /
• v.23 no.6
• /
• pp.612-618
• /
• 2012

In this paper, in order to prevent electric shock of high voltage system of HFCV after crash test, insulation performance measurement methods were studied. Under conditions of in-use, insulation performance tests can be divided into measurement method using the vehicle's own RESS as DC voltage source and measurement method using DC voltage from off-vehicle sources. However, these tests can not be applied after a post-crash because parts of high voltage system cover should be removed, and insulation performance can be influenced during these tests. Therefore, we proposed post-crash insulation performance test methods for preventing electric shock through problem analysis of previous post-crash insulation performance tests. Also, test equipment which can measure voltage absence and total energy was developed. We verified performance of the equipment through experiments with vehicle test.

• Journal of the Korean Society for Railway
• /
• v.13 no.4
• /
• pp.440-446
• /
• 2010

This paper was investigated on the laminated rubber bearing, which mitigates damages of bridges from threat of earthquakes. Laminated rubber bearing can bear large loads for long periods of time and be capable of large deformations during an earthquake. To evaluate seismic isolation with laminated rubber bearing on several earthquake waves, we performed a shaking table test. In this test, deck acceleration was measured by accelerometers and shear force on piers was surveyed by load cells. Furthermore, seismic capacity of isolated systems with laminated rubber bearing was compared with non-isolated systems through shaking table test. The results show that deck acceleration and shear force were relatively reduced by laminated rubber bearing.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.11 no.5
• /
• pp.131-139
• /
• 2001

In this paper, aerials gas pipeline vibrations due to the passing of vehicles have been measured, and peak values and characteristics of vibration were analyzed. In order to develop isolation system for gas pipeline, six cases with various support condition and hanging method were tested. The hanger used instead of wore rope isolated almost all vibrations transmitted from main beam. In the results of test, we could decrease response of vibration velocity from 49 % to 56 % and response of vertical vibration acceleration from 49 % to 60 %, because hanger instead of wire rope was used and rubber pads instead of wooden shield plates at support positions for Proofing horizontal vibration were used.

• Microbiology and Biotechnology Letters
• /
• v.22 no.1
• /
• pp.13-17
• /
• 1994

We estabilished the procedure for isolation of Streptococcus salivarius subsp. thermophilus from raw milk. First, urease-producing lactic acid bacteria in raw milk were screened on the HY agar medium containing urea. Thereafter the urease-producing colonies were tested the ability to ferment maltose and to grow at43$\circ $C. We obtained about 400 maltose-negative colonies that grew at 43$\circ $C. No significant difference in carbohydrate fermentation test for isolated and type strains(S. salivarius subsp. thermophilus ATCC 19258 and ST-4) was found. And all of the isolated strains were able to ferment galactose. Furthermore, it was investigated that the cellular fatty acid profiles of isolated strains were similar to that of type strains. These results indicated that the isolated strains from raw milk were S. salivarius subsp. thermophilus. But when the isolated and type strains were incubated in 12% reconstituted skim milk at 43$\circ $C, the isolates produced lactic acid more slowly than the type strains.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2001.04a
• /
• pp.426-431
• /
• 2001

Seismic performance of base isolated rigid-mass model were studied through shaking table tests. Friction pendulum systems (FPS), pure-friction systems with laminated rubber bearing (LRB) were selected for the comparison of performance. Performance of specially designed isolation systems were tested statically using actuator and dynamically using shaking table. Numerical methods were developed to simulate the nonlinear behavior of the frictional base isolation systems. Two models were considered. one is modified Bouc-Wen model considering breakaway coefficient of friction and the other is classical Coulomb model. The results of numerical methods are found to be in very good agreement with test results.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2009.04a
• /
• pp.524-525
• /
• 2009

Heavy-weight impact noise(HN) is the most irritating noise in Korean apartment houses. It has been proclaimed standard floor system of 210mm thick slab with isolation material in the wall type structure. But this regulation is applied only new construction field and is not considered remodeling field. In general, the LN can be reduced by using isolation material but HN is known as relating with stiffness, strength & boundary condition of slab. Therefore it is difficulty in improving the HN on remodeling field. In this study, We conducted the reinforcement of concrete slab using C.F.S.(carbon fiber sheet), steel plate and steel beam after on-dol with isolation material on the remodeling field. As the test results, It appeared using C.F.S was no improved but using steel plate & steel beam were a little improved on HN.