• Proceedings of the KOSOMBE Conference
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• v.1996 no.11
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• pp.277-280
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• 1996

We have developed a ground-isolation circuit in order to reduce the noise of the internal controller system for the total artificial heart(TAH) and ventricular assist device(VAD). Using the ground-isolation technique, we could transmit the analog target signal to other pheriperal device including IBM PC via RS232C and polygraph, with no noise. Experimental results of VAD showed that there was less impulsive noise in current signal which caused in our previous conventional system. Therefore it could be proved that implementation of isolation technique is very effective to improve the signal to noise ratios of analog signal transmission for TAH or VAD.

• Microbiology and Biotechnology Letters
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• v.31 no.4
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• pp.322-328
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• 2003

The Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5'and 3' gene targeting sequences. In this study, we examined the minimum size of specific hooks required for a single-copy gene isolation and compared the utility of different TAR vectors, radial and unique vectors, by cloning the same single-copy gene. The efficiency of TAR cloning of the hHPRT gene was same using hooks varying from 750 to 63 bp. The number of transformants decreased approximately 20-fold when the TAR vector contained two unique hooks versus using a radial vector, but the percentage of positive recombinants increased over 2-fold when a unique TAR vector was used. Therefore, we suggest that the two-unique TAR vector is suitable for general TAR cloning given its high selectivity, and the radial TAR vector is more suitable when genomic DNA is in limited quantity, for example, DNA isolated from pathological specimens. Moreover, we confirm the minimal length of a unique sequence in a TAR vector is approximately 60 bp for a single-copy gene isolation.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.2
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• pp.500-510
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• 1996

This paper presents a new prototype of an engine mount for a passenger vehicle featuring ER(elector-rheological) fluids and piezoactuators. Conventional rubber mounts and various types of passive or semi-active hydraulic engine mounts have their own functional aims on the limited frequency band in the board engine operating frequency range. However, the proposed engine mount covers all frequency range of the engine operation. A mathematical model of the proposed engine mount is derived using the bond graph method which is inherently domain, the ER fluid is activated upon imposing electric field for vibration isolation while the piezoactuator. Computer control electric fluid for the ER fluid H.inf. cotrol technique is adopted for the piezoactuator. Computer simulation is undertaken in order to demonstrate isolation efficiency of the engine mount over wide operating frequency range.

• Applied Biological Chemistry
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• v.23 no.4
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• pp.206-210
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• 1980

The minor components of $saponin-ginsenoside-Rf,\;-Rg_2\;and\;-Rh_1$ were isolated from Panax ginseng C.A. Meyer by preparative, semi-preparative and analtical high performance liquid chromatography. The rapid method developed in this work was proved to be very effective in separation and isolation of these minor ginsenosides. A further isolation was achieved by using the recycling technique.

• Journal of IKEEE
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• v.23 no.1
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• pp.266-272
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• 2019

Multicopters have become more popular since they are advantageous in their ability to take off and land vertically. In order to guarantee the normal operations of such multicopters, the problem of fault detection and isolation is very important. In this paper, a new method for detecting and isolating an actuator fault of a hexacopter is proposed based on the analytical approach. The residual is newly defined using the angular velocities of actuators estimated by the mathematical model and an actuator fault is detected comparing the residuals to a threshold. And a fault is isolated combining a dynamic model and generated residuals when a fault is detected. The proposed method is a simple, but effective technique because it is based on mathematical model. The results of the computer simulation are also given to demonstrate the validity of the proposed algorithm in case of a single failure.

• Smart Structures and Systems
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• v.24 no.5
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• pp.641-648
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• 2019

The authors investigate the feasibility of applying a vision-based displacement-measurement technique in the characterization of a SMA damper recently introduced in the literature. The experimental campaign tests a steel frame on a uni-axial shaking table driven by sinusoidal signals in the frequency range from 1Hz to 5Hz. Three different cameras are used to collect the images, namely an industrial camera and two commercial smartphones. The achieved results are compared. The camera showing the better performance is then used to test the same frame after its base isolation. U-shaped, shape-memory-alloy (SMA) elements are installed as dampers at the isolation level. The accelerations of the shaking table and those of the frame basement are measured by accelerometers. A system of markers is glued on these system components, as well as along the U-shaped elements serving as dampers. The different phases of the test are discussed, in the attempt to obtain as much possible information on the behavior of the SMA elements. Several tests were carried out until the thinner U-shaped element went to failure.

• Smart Structures and Systems
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• v.31 no.5
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• pp.531-543
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• 2023

Base isolation is one of the most widely implemented and well-known technique to reduce structural vibration and damages during an earthquake. However, while the base-isolated structure reduces storey drift significantly, it also increases the base drifts causing many practical problems. This study proposes the use of Shape Memory Alloys (SMA) wires for the reduction in base drift while controlling the overall structure vibrations. A multi-degree-of-freedom (MDOF) structure along with base isolators and Shape-Memory-Alloys (SMA) wires in diagonal is tested experimentally and analytically. The isolation bearing considered in this study consists of laminates of steel and silicon rubber. The performance of the proposed structure is evaluated and studied under different loadings including harmonic loading and seismic excitation. To assess the seismic performance of the proposed structure, shake table tests are conducted on base-isolated MDOF frame structure incorporating SMA wires, which is subjected to incremental harmonic and historic seismic loadings. Root mean square acceleration, displacement and drift are analyzed and discussed in detail for each story. To better understand the structure response, the percentage reduction of displacement is also determined for each story. The result shows that the reduction in the response of the proposed structure is much better than conventional base-isolated structure.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.3
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• pp.415-422
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• 2003

Currently, 'National program of dental sealant' has begun in Korea, but various isolation techniques and preparation is performing. The aim of the present study was to compare pit and fissure sealant retention rates between different operators using two different isolation techniques. Additionally, the failed surface was examined. One examiner performed the examination after one year of the initial operation. The population consisted of 119 children(mean age $8.59{\pm}0.772$) of which the total sealed number was 279 permanent first molars. Pediatric dentists sealed 131 molars using the rubber dam isolation technique (Group 1), general practitioners sealed 80 molars using the rubber dam isolation technique (Group 2), and dental hygienists sealed 69 molars using the cotton roll isolation (Group 3). The results were as follows ; 1. The complete retention rate between Group 1 (90.8%) and 2 (85.0%) showed no significant statistical difference (P>0.05). However, there were significant statistical different retention rates between Group 1 and Group 3(64.7%) and between Group 2 and Group 3 (p<0.05). 2. In comparing retention rates between maxilla and mandible, Only Group 3 showed a significantly lower complete retention rate in mandible than maxilla (p<0.05). 3. In failed surface analysis, occlusal failed surface was 58.3%, buccal/palatal failed surface was 41.7%.

• Korean Journal of Veterinary Research
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• v.13 no.1
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• pp.39-46
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• 1973

Cultural method, dark field microscopy & fluorescent antibody technique were compared for their sensitivity of the detection of leptospires from experimentally infected mice. Two groups of mice were infected with L. icterohemorrhagiae (M20) and L. australis (Ballico), and the infected blood, urine and a number of organs were subjected to the bacterial isolation. The results obtained were summarized as follows: 1. L. icterohemorrhagiae (M20) and L. australis (Ballico) in blood, urine and various tissues of experimentally infected mice were detected with a negrigible non specificity, by the fluorescent antibody technique. 2. The fluorescent antibody technique, as applied to detection of leptospires in blood, urine and various infected tissue, proved to be better than cultural method and dark-field microscopy. 3. Early detection of leptospires by fluorescent antibody technique were possible in blood at 2 days after inoculation, whereas detection of organisms in liver, spleen, lung and kidney were observed later. By means of fluorescent antibody technique, the detection of leptospires in kidney and urine was possible up to 34 days postinoculation, whereas those in other parts were impossible. 4. Fluorescent antibody reaction of leptospires were highly specific to homologous antigen rather than to heterologous one. 5. Fluorescent antibody technique may be of value in the application for the demonstration of leptospira from clinical specimens.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1380-1385
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• 2004

In this paper, auto-tuning technique of the PID controller gain by particle swarm optimization algorithm is presented. PID controller is easy to implement to numerous control systems. After PID gain tuning is completed, its result could be implemented to control spacecraft vibration such as jitter that is high frequency vibration usually over 10Hz. The off-line PID controller tuning is done under system nonlinearities and uncertainties existence, then its result is applied to control experiment device to prove the PSO efficiencies.