• Journal of Institute of Control, Robotics and Systems
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• v.14 no.1
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• pp.49-53
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• 2008

A fuzzy inference logic system is proposed for gas turbine engine fault isolation. The gas path measurements used for fault isolation are exhaust gas temperature, low and high rotor speed, and fuel flow. The fuzzy inference logic uses rules developed from a model of performance influence coefficients to isolate engine faults while accounting for uncertainty in gas path measurements. Inputs to the fuzzy inference logic system are measurement deviations of gas path parameters which are transferred directly from the ECM(Engine Control Monitoring) program and outputs are engine module faults. The proposed fuzzy inference logic system is tested using simulated data developed from the ECM trend plot reports and the results show that the proposed fuzzy inference logic system isolates module faults with high accuracy rate in the environment of high level of uncertainty.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.12
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• pp.856-865
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• 2015

In a high-speed press, numerous moving links are interconnected and each link executes a constrained motion at high speed. As a consequence, high-level dynamic unbalance force and unbalance moment are transmitted to the main frame of the press, which results in unwanted vibration and significantly degrades manufacturing accuracy. Dynamic unbalance force and unbalance moment inevitably transmits high-level vibrational force to the foundation on which the press is installed. Minimizing the vibrational force transmitted to the foundation is critical for the protection of both the operators and the surrounding structures. The whole task should be carried out in two steps. The first step is to reduce dynamic unbalance based upon kinematic and dynamic analyses. The second step is to design and build an optimal vibration isolation system minimizing the vibrational force transmitted to the foundation. Firstly, the dynamic design method is presented to reduce dynamic unbalance force and moment. For this a 3D CAD software was utilized and a computer program was written to compute dynamic unbalance force and moment. Secondly, the design method for vibration isolation system is presented. The method for designing coil springs and viscous dampers are explained in detail.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.305-313
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• 2008

The present study was conducted to investigate the epidemiological characteristics such as isolation rate and antimicrobial drug susceptibility of etiological agents. The specimens (stool or intestine) were collected from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. The isolation rate of Salmonella spp was higher in summer (8.4%) than in winter (4.8%) and the average was 7.2% (23/319 head). Some of Salmonella spp isolated were resistant to penicillin, oxytetracycline, tetracycline, and cephalexin (>90%), but some of them were susceptible to norfloxacin, ciprofloxacin, sulfamethoxazole/trimethoprim and amikacin(>30%). There was no statistical difference in the isolation rate of Eimeria spp between summer(48.9%) and winter(42.3%). For the evaluation of infection level of Eimeria spp oocyst per gram of feces (OPG) was examined, and severe, moderate and light infection level were 11.9%, 12.5% and 22.3%, respectively. In the isolation rates of Eimeria spp the calves under 19 days was lowerthan those over 60 days, but there was not different among herd size.

• Earthquakes and Structures
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• v.13 no.3
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• pp.267-277
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• 2017

Base-isolation is now being adopted as a retrofitting strategy to improve seismic behaviour of reinforced concrete (r.c.) framed structures subjected to far-fault earthquakes. However, the increase in deformability of a base-isolated framed building may lead to amplification in the structural response under the long-duration horizontal pulses of high-magnitude near-fault earthquakes, which can become critical once the strength level of a fire-weakened r.c. superstructure is reduced. The aim of the present work is to investigate the nonlinear seismic response of fire-damaged r.c. framed structures retrofitted by base-isolation. For this purpose, a five-storey r.c. framed building primarily designed (as fixed-base) in compliance with a former Italian seismic code for a medium-risk zone, is to be retrofitted by the insertion of elastomeric bearings to meet the requirements of the current Italian code in a high-risk seismic zone. The nonlinear seismic response of the original (fixed-base) and retrofitted (base-isolated) test structures in a no fire situation are compared with those in the event of fire in the superstructure, where parametric temperature-time curves are defined at the first level, the first two and the upper levels. A lumped plasticity model describes the inelastic behaviour of the fire-damaged r.c. frame members, while a nonlinear force-displacement law is adopted for the elastomeric bearings. The average root-mean-square deviation of the observed spectrum from the target design spectrum together with a suitable intensity measure are chosen to select and scale near- and far-fault earthquakes on the basis of the design hypotheses adopted.

• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.178-184
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• 1996

Dryfilm method by using 3M Petrifilm$^{TM}$ has been examined to replace conventional agar method for isolation of microorganisms from foods. The objectives of the present study were to evaluate suitability of dryfilm method as a microbial isolation method and to determine the effect of antimicrobial agent on dryfilm for isolation of microorganisms from foods. Five different foods, milk, ground beef, fishery surimi, Takju and wheat flour were used to isolate the natural microflora in foods and the inoculated Escheri chia coli. Standard method agar (SMA, Difco) and Petrifilm$^{TM}$ aerobic count (PAC, 3M) were used to isolate total microorganisms from foods. Violet red bile agar (VRBA), brilliant green lactose bile (BGLB) broth and Petrifilm$^{TM}$ coliform count (PCC, 3M) were used to isolate coliforms from foods. E. coli broth (EC broth) and Petrifilm$^{TM}$ E. coli count (PEC, 3M) were used to isolate E. coli from foods. Acidified potato dextrose agar (APDA) and Petrifilm$^{TM}$ yeast & mold count (PYMC, 3M) were used to isolate yeasts and molds from foods. Total aerobic plate counts isolated from five different foods by SMA and PAC (3M) were riot significantly different each other at P<0.05 level and were highly correlated each other ($\geq$0.96). Mugwort extract as an antimicrobial agent did not affect microbial enumeratiion of Dryfilm. Significantly higher number of coliform colonies were formed on VRBA than PCC (3M) from ground beef, but they were not significantly different in coliform colonies from milk samples. PCC (3M) and BGLB were not significantly different for enumeration of coliforms in milk and beef samples. Significantly higher number of E. coli were isolated by EC broth than PEC from ground beef, but these were not significontly different for enumeration of E. coli from milk. Yeast and mold counts isolated from Takju and wheat flour by APDA and PYMC (3M) were not significantly different at P<0.05 level. These data indicate that dryfilm method by using 3M Petrifilm$^{TM}$ can be successively used as an alternative to conventional agar method for enumeration of microorganisms in various foods.

• The Korean Journal of Elementary Counseling
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• v.6 no.1
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• pp.217-245
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• 2007

This research work was aimed at assisting isolated children to adapt their school life having examined the process of their changes by reducing isolation level and isolative behavior through means of implementing group art therapy program. To accomplish this purpose of research, 36 participants were selected from children attending Class 6, 4th Grade of D Elementary School located in Dajeon Metropolitan City, to which researcher was served as homeroom teacher. And the participants were asked to complete a survey that included social and emotional isolation scale test which was designed by Heh Sung-hee(1993) and 4 children marked more than 96 points were designated as isolated children. The group art therapy program exercised in this research was the one that reorganized by research to suit the research purpose based on the precedent works, and survey was conducted total 12 times, two time a week, within 60 minutes per time, between 6 September and 24 October 2006, and researcher conducted group art therapy program directly at Class 6, 4th Grade of D Elementary School using extra hours after school. And following analyses were performed in relation to results therefrom: First, to analyze changes of isolation level of isolated children Quantitatively, the results from pre and post test concerning social and emotional isolation scale were comparatively analyzed. Second, to analyze changes of psychological state of isolated children, projective drawing tests were performed using Kinetic House-Tree-Person(K-HTP) Drawing and Kinetic School Drawing(KSD) and results therefrom were comparatively analyzed. Third, to analyze Quantitatively what changes have been occurred in the isolated children during the course of forming interrelationships with group members while participating in group art therapy, proceedings were summarized per each stage of the program and analyzed accordingly. Fourth, results from researcher's observation of what changes have been occurred in the isolative behavior of isolated children before and after implementation of group art therapy were compared accordingly. Followings are resulting conclusions. First, group art therapy program reduced isolation level of isolated children. Second, group art therapy program changed the isolated children's psychological state into a stable and positive fashion. Third, isolated children became able to experience positive interrelations as well as establishment of new human relationships with group members during the course of participating in the group art therapy program. Fourth, group art therapy program reduced isolative behaviors of the isolated children. Therefore, conclusions could have been reached that the group art therapy program implemented in this research reduced isolative behaviors of the isolated children, and changed their anxious and depressed mental state into stable and positive ones, also established positive interrelationships as well as new human relationships. And, it will be able to expect a greater effect if a program which has been associated with their family could be proceeded along with continuous guidance and teaching having developed objective test tools that will discern isolated children.

• Journal of Marine Bioscience and Biotechnology
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• v.12 no.1
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• pp.50-58
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• 2020

Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts ("naked" plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.42 no.8
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• pp.648-653
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• 2014

Fly-wheel, gimbal antenna, mechanical gyro and cryocooler with moving parts generate a micro-vibration during their on-orbit operation. For the acquisition of high quality image of observation satellite, additional technical efforts are required to reduce the micro-vibration level from the vibration sources. In this study, we proposed a passive isolation system combined with a tuned mass damper-type energy harvester to generate electrical energy from the micro-vibration which has always been subjected to useless isolation objectives. The feasibility of the system has been investigated through the numerical simulation.

• Journal of the Korean Society for Precision Engineering
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• v.27 no.7
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• pp.13-20
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• 2010

Due to the recent quantum leaps forward in bio-, nano-, and information-technologies, the precisionization and miniaturization of mechanical and electrical components are in high demand. The allowable margin for vibration limits for such equipments is becoming stricter. In order to meet this demand, understandings on the characteristics of vibration isolation systems are highly required. Among the components comprising the vibration isolation system, air spring has become a focal point. In order to develop a complex defect tester for COG bonding of display panels, a vibration isolation system composed of air springs for mounting is considered in this study. The dynamic characteristics of the air spring are investigated, which is the most essential ingredient for reducing the vibration problem of the tester to the lowest level. Uncoupled dynamic parameters of the air spring are identified through MTS experiments, followed by suggestion of a model-based approach to obtain the remaining coupled dynamic parameters. Finally, the dynamic behaviors of the air spring are estimated and discussed.

• Journal of the Earthquake Engineering Society of Korea
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• v.24 no.4
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• pp.169-178
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• 2020

In a previous paper, ambient vibration tests were conducted on a cable stayed bridge with resilient-friction base isolation systems (R-FBI) to extract the dynamic characteristics of the bridge and compare the results with a seismic analysis model. In this paper, a nonlinear seismic analysis model was established for analysis of the bridge to compare the difference in seismic responses between nonlinear time history analysis and multi-mode spectral analysis methods in the seismic design phase of cable supported bridges. Through these studies, it was confirmed that the seismic design procedures of the "Korean Highway Bridge Design Code (Limit State Design) for Cable Supported Bridges" is not suitable for cable supported bridges installed with R-FBI. Therefore, to reflect the actual dynamic characteristics of the R-FBI installed on cable-supported bridges, an improved seismic design procedure is proposed that applies the seismic analysis method differently depending on the seismic isolation effect of the R-FBI for each seismic performance level.