• Korean Journal of Microbiology
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• v.21 no.4
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• pp.197-206
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• 1983

For the purpose of investigating the effect of nalidixic acid on the nucleic acid synthesis in chloroplast isolated from Chlorella ellipsoidea, cells were cultured in the media treated with nalidixic acid(20ppm) for 5 days. Aliquots cells were taken out at the inoculation and at intervals during the culture and growth rate of Chlorella cells measured. After extraction of nucleic acids in chloroplast isolated from these cells, their contents were analyzed by the base composition and the effect of nalidixic acid on the nucleic acid synthesis interpreted to compare with those of the control. 1. It was showed that the inhibitory concentration affected by nalidixic acid on the growth of Chlorella cells were 20ppm. 2. Because nalidixic acid had depressed the DNA replication in isolated chloroplast as well as whole cell system, these contents were markedly decreased in comparison with those of the control. 3. In the isolated chloroplast as well as in the whole cell system, nalidixic acid was decreased contents of base in the RNA by preventing RNA transcription.

• Journal of the Earthquake Engineering Society of Korea
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• v.19 no.6
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• pp.293-302
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• 2015

ASCE 4 requires that a hard stop be built around the seismic isolation system in nuclear power plants. In order to maintain the function of the isolation system, this hard stop is required to have clearance-to-stop, which should be no less than the 90th-percentile displacements for 150% Design Basis Earthquake (DBE) shaking. Huang et al. calculated clearance-to-stop by using a Latin Hypercube Sampling technique, without considering the rocking behavior of the isolated structure. This paper investigates the effects on estimation of clearance-to-stop due to 1) rocking behavior of the isolated structure and 2) sampling technique for considering the uncertainties of isolation system. This paper explains the simplified analysis model to consider the rocking behavior of the isolated structure, and the input earthquakes recorded at Diablo Canyon in the western United States. In order to more accurately approximate the distribution tail of the horizontal displacement in the isolated structure, a modified Latin Hypercube Sampling technique is proposed, and then this technique was applied to consider the uncertainty of the isolation system. Through the use of this technique, it was found that rocking behavior has no significant effect on horizontal displacement (and thus clearance-to-stop) of the isolated structure, and the modified Latin Hypercube Sampling technique more accurately approximates the distribution tail of the horizontal displacement than the existing Latin Hypercube Sampling technique.

• Journal of the Earthquake Engineering Society of Korea
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• v.17 no.6
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• pp.245-255
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• 2013

According to natural frequency of soil, characteristics of earthquake responses of an isolated containment building in nuclear power plants are examined. For this, earthquake response analysis of seismically isolated containment buildings in nuclear power plants is carried out by strictly considering soil-structure interactions. The structure and near-field soil are modeled by the finite element method while far-field soil by consistent transmitting boundary. The equation of motion of a soil-structure interaction system under incident seismic wave is derived. The derived equations of motion are solved to carry out earthquake analysis of a seismically isolated soil-structure system. Generally, the results of this analysis show that seismic isolation significantly reduces the responses of the soil-structure system. However, if the natural frequency of the soil is similar to that of the soil-structure system, the responses of the containment buildings in nuclear power plants rather increases due to interactions in the system.

• Earthquakes and Structures
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• v.4 no.5
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• pp.471-487
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• 2013

Performance of a prototype base isolated building located at Indian Institute of Technology, Guwahati (IITG) has been studied here. Two numbers of three storeyed single bay RCC framed prototype buildings were constructed for experimental purpose at IITG, one supported on conventional isolated footings and the other on a seismic isolation system, consisting of lead plug bearings. Force balance accelerometers and a 12 channel strong motion recorder have been used for recording building response during seismic events. Floor responses from these buildings show amplification for the conventional building while 60 to 70% reduction has been observed for the isolated building. Numerical models of both the buildings have been created in SAP2000 Nonlinear. Infill walls have been modeled as compression struts and have been incorporated into the 3D models using Gap elements. System identification of the recorded data has been carried out using Parametric State Space Modeling (N4SID) and the numerical models have been updated accordingly. The study demonstrates the effectiveness of base isolation systems in controlling seismic response of isolated buildings thereby leading to increased levels of seismic protection. The numerical models calibrated by relatively low level of earthquake shaking provides the starting point for modeling the non-linear response of the building when subjected to strong shaking.

• Structural Engineering and Mechanics
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• v.35 no.6
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• pp.759-772
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• 2010

Seismic isolation is a well-known method to mitigate the earthquake effects on structures by increasing their fundamental natural periods at the expense of larger displacements in the structural system. In this paper, the seismic response of isolated and fixed base vertical, cylindrical, liquid storage tanks is investigated using a Finite Element Model (FEM), taking into account fluid-structure interaction effects. Three vertical, cylindrical tanks with different ratios of height to radius (H/R = 2.6, 1.0 and 0.3) are numerically analyzed and the results of response-history analysis, including base shear, overturning moment and free surface displacement are reported for isolated and non-isolated tanks. Isolated tanks equipped by lead rubber bearings isolators and the bearing are modeled by using a non-linear spring in FEM model. It is observed that the seismic isolation of liquid storage tanks is quite effective and the response of isolated tanks is significantly influenced by the system parameters such as their fundamental frequencies and the aspect ratio of the tanks. However, the base isolation does not significantly affect the surface wave height and even it can causes adverse effects on the free surface sloshing motion.

• Earthquakes and Structures
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• v.5 no.4
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• pp.461-475
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• 2013

A new experimental system of base-isolated structures is proposed. There are two kinds of dampers usually used in the base-isolated buildings, one is a viscous-type damper and the other is an elastic-plastic hysteretic-type damper. The base-isolated structure with a viscous damper and that with an elastic-plastic hysteretic damper are compared in this paper. The viscous damper is modeled by a mini piston and the elastic-plastic hysteretic damper is modeled by a low yield-point steel. The capacity of both dampers is determined so that the dissipated energies are equivalent at a specified deformation. When the capacity of both dampers is determined according to this criterion, it is shown that the response of the base-isolated structure with the elastic-plastic hysteretic damper is larger than that with the viscous damper. This characteristic is demonstrated through the comparison of the bound of the aspect ratio. It is shown that the bound of aspect ratio for the base-isolated structure with the elastic-plastic hysteretic damper is generally smaller than that with the viscous damper. When the base-isolated structure is subjected to long-duration input, the mechanical property of the elastic-plastic hysteretic damper deteriorates and the response of the base-isolated structure including that damper becomes larger than that with the viscous damper. The effect of this change of material properties on the response of the base-isolated structure is also investigated.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.11a
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• pp.247-251
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• 2001

Nowadays, base isolation systems such as lead-rubber bearing, elastomer bearing and sliding bearing have been installed to the various structures to prevent the disaster from seismic. The performance of base isolation system have been well proved by model-scale experiments and numerical analysis. However. the seismic response data measured at real large base-isolated structures is still insufficient. This paper presents a seismic monitoring system, acquiring real-time acceleration signals up to 32 channels, displaying time history and spectrum of the signals, storing the acquired data at a PC hard disk, and replaying the saved data. Moreover, the system can be operated without any limitation for monitoring period by automatic management of stored data file. The developed system has been installed at a real base-isolated building using lead-rubber bearings and we expect its seismic response data with ground motion signal can be well licquired in case of earthquake occurrence.

• Earthquakes and Structures
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• v.14 no.5
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• pp.389-398
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• 2018

The seismic isolation system makes a structure isolated from ground motions to protect the structure from seismic events. Seismic isolation techniques have been implemented in full-scale buildings and bridges because of their simplicity, economic effectiveness, inherent stability and reliability. As for the responses of an isolated structure due to seismic events, it is well known that the most uncertain aspects are the seismic loading itself and structural properties. Due to the randomness of earthquakes and uncertainty of structures, seismic response distributions of an isolated structure are needed when evaluating the seismic fragility assessment (or probabilistic seismic safety assessment) of an isolated structure. Seismic response time histories are useful and often essential elements in its design or evaluation stage. Thus, a large number of non-linear dynamic analyses should be performed to evaluate the seismic performance of an isolated structure. However, it is a monumental task to gather the design or evaluation information of the isolated structure from too many seismic analyses, which is impractical. In this paper, a new methodology that can evaluate the seismic fragility assessment of an isolated structure is proposed by using stochastic response database, which is a device that can estimate the seismic response distributions of an isolated structure without any seismic response analyses. The seismic fragility assessment of the isolated nuclear power plant is performed using the proposed methodology. The proposed methodology is able to evaluate the seismic performance of isolated structures effectively and reduce the computational efforts tremendously.

• Smart Structures and Systems
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• v.23 no.1
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• pp.91-106
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• 2019

Adopting sloped rolling-type isolation devices underneath a raised floor system has been proved as one of the most effective approaches to mitigate seismic responses of the protected equipment installed above. However, pounding against surrounding walls or other obstructions may occur if such a base-isolated raised floor system is subjected to long-period excitation, leading to adverse effects or even more severe damage. In this study, real-time hybrid simulation (RTHS) is adopted to assess the control performance of a smart base-isolated raised floor system as it is an efficient and cost-effective experimental method. It is composed of multiple sloped rolling-type isolation devices, a rigid steel platen, four magnetorheological (MR) dampers, and protected high-tech equipment. One of the MR dampers is physically tested in the laboratory while the remainders are numerically simulated. In order to consider the effect of input excitation characteristics on the isolation performance, the smart base-isolated raised floor system is assumed to be located at the roof of a building and the ground level. Four control algorithms are designed for the MR dampers including passive-on, switching, modified switching, and fuzzy logic control. Six artificial spectrum-compatible input excitations and three slope angles of the isolation devices are considered in the RTHS. Experimental results demonstrate that the incorporation of semi-active control into a base-isolated raised floor system is effective and feasible in practice for high-tech industry.

• Structural Engineering and Mechanics
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• v.64 no.4
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• pp.473-485
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• 2017

An isolated building, composed of superstructure and isolation system which have very different damping properties, is typically non-classical damping system. This results in inapplicability of traditional response spectrum method for isolated buildings. A multidimensional response spectrum method based on complex mode superposition is herein introduced, which properly takes into account the non-classical damping feature in the structure and a new method is developed to estimate velocity spectra from the commonly used displacement or pseudo-acceleration spectra based on random vibration theory. The error of forced decoupling method, an approximated approach, is discussed in the viewpoint of energy transfer. From the base-isolated benchmark model, as a numerical example, application of the procedure is illustrated companying with comparison study of time-history method, forced decoupling method and the proposed method. The results show that the proposed method is valid, while forced decoupling approach can't reflect the characteristics of isolated buildings and may lead to insecurity of structures.