• Molecules and Cells
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• v.19 no.2
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• pp.212-218
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• 2005

A full-length 1.1 kb cDNA, designated Oryza sativa Dehydrin 1 (OsDhn1), was isolated from the seed coat of rice. The deduced protein is hydrophilic and has three K-type and one S-type motifs (SK3-type), indicating that OsDhn1 belongs to the acidic dehydrin family, which includes wheat WCOR410 and Arabidopsis COR47. Expression of OsDhn1 was strongly induced by low temperature as well as by drought. Induction of OsDhn1 by cold stress was clearcut in the roots of seedlings and the epidermis of palea and lemma. OsDhn1 was also up-regulated in UBI::CBF1/DREB1b transgenic plants indicating that it is regulated by the CBF/DREB stress signaling pathway.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.347-349
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• 2001

Black blights attacked the blossom and flower buds of wild chrysanthemum (Chrysanthemum boreale) in the experimental field in Hamyang in 1998. The infection rate of the disease on the plant ranged from 4.0 to 91.8%. The pathogen isolated from the infected flower buds produced numerous conidia in pycnidia. The pycnidia, which were immersed into the petals, emerged through the epidermis by short ostiolate neck. Conidia had 0-3 septate (mostly uniseptate) and were 10-27.5 $\times$5-7.5 ㎛ in size. The fungus produced pseudothecia on potato dextrose agar (PDA), and uniseptate ascospores produced in asci were 10$\times$2.7 ㎛ in size. The pathogen also produced pycnidia and pycnidiospores on PDA after 4 weeks in the dark condition. The conidia produced on PDA were smaller than those from infected plants. Based on the examined mycological characteristics, the fungus was identified as Didymella chrsanthemi.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.123-126
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• 2004

The actin-binding protein profilin cDNA was firstly isolated from the lepidopteran insect, silkworm Bombyx mori. The B. mori profilin cDNA contains an open reading frame of 378 bp encoding 126 amino acid residues and possesses three cysteine residues. The deduced amino acid sequence of the B. mori profilin cDNA showed 80% identity to Apis mellifera profilin and 72% to Drosophila melanogaster profilin. Northern blot analysis showed that B. mori profilin is highly expressed in epidermis and less strongly in silk gland. In addition, Northern blot analysis revealed the presence of B. mori profilin transcripts in all tissues examined, suggesting that B. mori profilin gene is expressed in most, if not all, body tissues.

• Korean Journal of Veterinary Service
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• v.14 no.2
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• pp.134-142
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• 1991

We surveyed the antibody titers in the slaughtered pigs by enzyme immunodiffusion method for the epidemiological distribution of Aujeszky's disease in Korea. And also we observed the clinical symptoms, histopathological findings and antibody titers by serologic neutralization test in experimental animals infected with Aujeszky's disease virus(ADV) isolated from Korea. The results of the experiments were summarized as follows. 1. We detected 2 pigs infected with ADV of 1000 in 1990 and 1 of 600 in 1991 by enzyme immunodiffusion method. 2. In histopathological findings of experimental animals inoculated ADV, the typical characteristics of Aujeszky's disease were not observed in pig, but edema and degeneration in the epidermis were observed in rats, vasculoendotheriosis, perivascular cuffing and cellular infiltration in the cerebrum were appeared in rabbits and perivascular cuffing and forcal infiltration of glial cells in the cerebrum were appeared in mice. 3. The increasing antibody titers(1 : 64) in the serological neutralization test were ascertained in 2 carrier pigs inoculated ADV. 4. Rabbits, mice and rats died all about 5 days after inoculation, but pigs and chickens didn't die. 5. In 1 cat and 2 rabbits inoculated with the Aujeszky's disease virus(ADV), the typical clinical findings of Aujeszky's disease were observed in rabbit, but not in cat and so we slaughtered without any signs of Aujeszky's disease.

• Journal of fish pathology
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• v.6 no.2
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• pp.197-204
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• 1993

Flounder culture has been developed mainly in the western parts of japan, and, to date, following six bacterial diseases have been reported. Bacterial white enteritis occurs in 16 to 30-day-old flounder larvae and often causes mass mortality in seed production. Bacterium named Vibrio sp. INFL invades and multiplies in the mucosae of posterier part of intestine, and causes desquamative enteritis. Gliding bacterial disease occurs mostly in juvenile stage and in spring to summer. Diseased signs are partial discoloration and erosion of skin and fins. Histologically, epidermis are removed, and the causative bacterium, Flexibacter maritimus, multiplies on the surface of demis and invades into the muscular tissue. Vibriosis caused by Vibrio anguillarum and related organisum is one of the well-known diseases among marine fish. Outbreaks of the disease in flounder culture are relatively few, but mass mortalities in fingerlings due to the disease were reported. An outbreak of nocardiosis in the autumn of 1984 has been reported, but since then the disease scarcely occurred. The disease is characterized by formation of abscesses under the skin and white nodes in the gill, heart, spleen and kidney. Streptococcicosis occurs frequently in recent years. Beta-hemolytic streptococcus is the causative bacterium, which possesses the same biochemical and serological characteristics as $\beta$-streptococci isolated from some marine and freshwater fish, and is seemed to related to Streptococcus iniae. Edwardsiellosis is the disease that causes most damage in flounder culture in Japan. Characteristic symptoms are swelling of abdomen and intestinal protrusion from the anus due to accumulation of ascites. Edwardsiella tarda, a well-known pathogen of freshwater fish, is the causative bacterium of the disease.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.67-70
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• 2005

A cuticle protein gene, PbLCP12.1, from the white­spotted flower chafer, Protaetia brevitarsis, was isolated and characterized. The gene contains an ORF of 336 nucleotides capable of encoding a 113 amino acid polypeptide with a predicted molecular mass of 12,138 Da and pI of 4.15. The PbLCP12.1 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins. The deduced amino acid sequence of the PbLCP12.1 cDNA is most similar to Bombyx mori cuticle protein BmLCP18 (37$\%$ protein sequence identity). Northern blot analysis revealed that PbLCP12.1 showed the epidermis-specific expression.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.176-181
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• 2005

To study calmodulin (CaM) gene expression and its regulation, rice CaM promoter (RCaM-2) was isolated and fused to $\beta-glucuronidase$ (GUS), reporter gene. X-Glue staining patterns revealed that GUS localization is high in meristemic tissues such as the stem apex, stolen tip, and vascular regions. GUS staining in the transverse sections of stem and petiole was restricted to the inside of the vascular system, and cortex and epidermis located outside of the vascular system usually did not show GUS staining even a plant that expressed strong activity. GUS activity was found to be tissue specific expressed and exhibited a dramatic transient increase in response to wounding. These results suggest that the 5'-flanking region of RCaM gene regulates wound-inducible expression.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.553-562
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• 1995

To elucidate pathologic change of skin in porcine exudative epidermitis, immunohistochemical and electron microscopical observations were carried out in the skin of the suckling pigs inoculated with Staphylococcus hyicus subsp hyicus which were isolated from natural case. In immunohistochemistry, ATPase-positive dendritic cells were more populated in epidermo-dermal junctional areas and perivascular area in dermis than in epidermal area as the disease was proceeded. These dendritic cells were identified as Langerhans cell by immunoperoxidase staining and these cells were populated granulomatous bodies. Electron microscopical study showed various retrogressive degeneration and vacuolation of epidermal cell organelles with retention of amorphorous exudates in intercellular space, and cellular seperation. Langerhans cells present in intercellular space of epidermis were populated in epidermo-dermal junctional areas, in dermis, and around granulomatous bodies. Langerhans cells contained decreased Birbeck granules in number but increased lysosome and ribosome. These cells were in contact with lymphocytes. This study was discussed relation between the various immunocytes and the formation of granulomatous bodies, and this inflammation was considered as delayed type hypersensitivity.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.107-124
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• 2000

A bacterial strain No. HB-5, which was capable of producing a pretense in the culture conditions, was isolated from the soil . The pretense was purified from cultural filtrate of Bacillus sp. HB-5 by membrane ultrafiltration and DEAE- cellulose chromatography, gel filtration on Sephadex G-100. The molecular weight was estimated to be 60k4a. The optimal pH and temperature for the activity of the purified pretense pH were 11 and 5$0^{\circ}C$ , respectively. The enzyme was stable within a pH range 8-12 and up to 6$0^{\circ}C$ . The enzyme activity was highly inhibited by PMSF at 1mM. The proteolytic actions of pretense and papain on human epidermis keratins which are major protein impurities on the skin, were compared. The bacterial pretense degraded more effectively than papain. Product containing 2% protease exhibited 21% increase on the skin coloration index. These results suggest that cosmetic product containing pretense produced by Bacillus sp. HB-5 could remove the adherent keratin layer and then make a softer skin.

• Research in Plant Disease
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• v.26 no.1
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• pp.29-37
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• 2020

Stem blight symptom of soybean was severely developed in 2016 in Hwaseong and Yeoncheon. During the seedling period, the damping-off of seedlings and the brown or black spots of cotyledons were observed. After August, the leaves began to be yellowed, and partially browned areas on leaves began to develop. After September, microsclerotia began to form even on the surface of the stems that had exhibited water-soaking symptom. After mid-October of the harvest season, the epidermis of the stem was peeled off, resulting in the formation of a large number of microsclerotia in the cortex. The pathogens isolated from these symptoms were the best in mycelial growth at 32-35℃, and the formation of microsclerotia was the most at 20-28℃. The pathogen was identified as Macrophomina phaseolina through the morphological characteristics of the pathogen and the sequencing of the internal transcribed spacer region gene. In addition, when inoculated with a soybean stem using toothpicks cultured with the pathogen, the same symptoms as seen on the soybean field occurred. When the pathogen was re-isolated at the lesion site, the same pathogen was isolated and identified as Macrophomina phaseolina. Based on the results, the disease is reported as soybean charcoal rot.