• Title/Summary/Keyword: Irradiation intensity

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Effect of the 100Hz PWM Low Power Light Irradiation in Proliferation of NTacSam:SD Bone-marrow Cell (NTacSam:SD 골수 세포의 증식에 100Hz PWM 저출력 광 조사가 미치는 효과)

  • Cheon, Min-Woo;Kim, Seong-Hwan;Lee, Ho-Sic;Park, Yong-Pil
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.04b
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    • pp.10-11
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    • 2008
  • We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Control stage is divided into 30 levels by program. Consequently, the current value could be controlled by the change of level in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage. And then, each experiment was performed to irradiation group and non-irradiation group for bone marrow cells. MIT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590nm transmittance of ELISA reader. As a result, the cell increase of bone marrow cells was verified in irradiation group as compared to non-irradiation group.

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The Effect of Distance between Two Transducers on Sonochemical Reactions in Dual Irradiation Systems (이중 초음파 조사 시스템에서 진동부 사이의 거리가 초음파 화학 반응에 미치는 영향)

  • Kim, Eunkyung;Son, Younggyu
    • Journal of Soil and Groundwater Environment
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    • v.18 no.5
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    • pp.39-45
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    • 2013
  • Many researchers have studied the effectiveness of ultrasound in chemical and environmental engineering fields including material synthesis, pollutant removal, cleaning, extraction, and disinfection. Acoustic cavitation induced by ultrasound irradiation in aqueous phase can cause various sonophysical and sonochemical reactions without any chemicals. However most of the previous studies focused only on the relationships between ultrasonic conditions and the results of sonochemical reactions in lab-scale sonoreactors. As a results of this, only a few studies have been devoted to design and optimization of industrial scale sonoreactors. In this study, the effect of the distance between two opposite transducer modules on sonochemical reactions was investigated in single and dual irradiation systems (334 kHz) for four distances including 50, 100, 150, and 200 mm using KI dosimetry. It was found that the dual irradiation systems provided higher performance in terms of the zeroth reaction coefficient and the cavitation yield compared to the single irradiation systems. The sonochemiluminescence (SCL) images for the visualization of the cavitation field showed that cavitation active zone was larger and sonochemical reaction intensity was much higher in the dual irradiation system than in the single irradiation system.

Effect of light intensity on the ozone formation and the aerosol number concentration of ambient air in Seoul (광도가 서울 대기의 오존 생성 및 에어로졸 수 농도에 미치는 영향)

  • Bae, Gwi-Nam;Park, Ju-Yeon;Kim, Min Cheol;Lee, Seung-Bok;Moon, Kil-Choo;Kim, Yong Pyo
    • Particle and aerosol research
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    • v.4 no.1
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    • pp.9-20
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    • 2008
  • The effect of light intensity on the ozone formation and the aerosol number concentration during the photochemical reactions of ambient air was investigated in an indoor smog chamber. The smog chamber consists of a housing, 64 blacklights, and a $2.5-m^3$ reaction bag made of Teflon film. The bag was filled with the unfiltered ambient air in Seoul from January 10 to March 18, 2002. In this work, the photolysis rate of $NO_2$, $k_1$ was used as an index of light intensity. Three levels of light intensity were controlled by changing the number of blacklights turned on among 64 blacklights: $0.29min^{-1}$ (50%), $0.44min^{-1}$ (75%), $0.57min^{-1}$ (100%). The ozone concentration increased rapidly within 10 minutes after irradiation irrespective of light intensity, thereafter it increased linearly during the irradiation. The ozone production rate seems to be dependent on both the light intensity and the quality of ambient air introduced into the reaction bag. The change in aerosol number concentration also depended on both the light intensity and the ambient air quality, especially aerosol size distribution. Based on the initial ambient aerosol size distributions, the photochemical potential for aerosol formation and growth is classified into two cases. One is the case showing aerosol formation and growth processes, and the other is the case showing no apparent change in particle size distribution.

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Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato (자색고구마 캘러스배양에서 안토시아닌 생합성에 미치는 광의 영향)

  • Park Hyae-Jeong;Kim Jung-Suk;Park Hyeon-Yong
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.307-311
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    • 2005
  • The anthocyanin biosynthesis in callus culture of purple sweet potato (Ipomoea batatas L. cv. Borami) was investigated under growth in different light intensity and light emitting diodes (LED) treatment. Pigmented calli were induced from leaf explants cultured on MS agar medium supplemented with $0.5\;{\mu}M$ 2,4-D under light condition. The color value of these calli extracted after $2{\sim}4$ weeks of cultures was $0.4{\sim}0.5\;mg/mL$. Irradiation intensity is an important factor for anthocyanin biosynthesis. The optimal anthocyanin accumulation occurred on light intensity of 3000 lux. Light irradiation of 3000 lux and blue light treatment for 2 h resulted in a significant enhancement of anthocyanin accumulation. This value was 1.4 fold that the control.

Effect of γ-Irradiation on the Molecular Properties of Bovine Serum Albumin and β-Lcatoglobulin

  • Cho, Yong-Sik;Song, Kyung-Bin
    • BMB Reports
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    • v.33 no.2
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    • pp.133-137
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    • 2000
  • To elucidate the effect of oxygen radicals on the molecular properties of proteins, the secondary and tertiary structure and molecular weight size of BSA and ${\beta}$-lactoglobulin were examined after irradiation of proteins at various doses. Gamma-irradiation of protein solutions caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking, and aggregation of the polypeptide chains. As a model system, BSA and ${\beta}$-lactoglobulin were used as a typical ${\alpha}$-helical and a ${\beta}$-sheet structure protein, respectively. A circular dichroism study showed that the increase of radiation decreased the ordered structure of proteins with a concurrent increase of aperiodic structure content. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. SDS-PAGE and a gel permeation chromatography study indicated that radiation caused initial fragmentation of proteins resulting in a subsequent aggregation due to cross-linking of protein molecules.

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Stabilization of pitch-based carbon fibers accompanying electron beam irradiation and their mechanical properties

  • Park, Mi-Seon;Ko, Yoonyoung;Jung, Min-Jung;Lee, Young-Seak
    • Carbon letters
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    • v.16 no.2
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    • pp.121-126
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    • 2015
  • Carbon fibers are prepared by stabilizing pitch fibers accompanying electron beam (E-beam) irradiation. The carbon fibers pretreated by E-beam irradiation achieve a higher stabilization index than the carbon fibers that are only heat-stabilized. In addition, the carbon fibers subjected to E-beam irradiation in the stabilization step exhibit a comparable tensile strength to that of general purpose carbon fibers. The carbon fibers pretreated with an absorbed dose of 3000 kGy have a tensile strength of 0.54 GPa for a similar fiber diameter. Elemental, Fourier-transform infrared spectroscopy, and thermogravimetric analyses indicate that E-beam irradiation is an efficient oxidation and dehydrogenation treatment for pitch fibers by showing that the intensity of the aliphatic C-H stretching and aromatic $CH_2$ bending (out-of-plane) bands significantly decrease and carbonyl and carboxylic groups form.

The Study on Wound Healing in Rabbit Skins by Low-intensity Laser Irradiation (저강도 레이저 조사에 의한 가토 피부의 상처 치유에 관한 연구)

  • 김식현;전진석
    • Biomedical Science Letters
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    • v.6 no.2
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    • pp.119-129
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    • 2000
  • The skin is an organ that has many important roles, including protection against infection, regulation of temperature and fluid loss, and sensory function. Injury to the skin, wound repair normally involves: (1) balanced activity of inflammation, (2) the re-epithelial phase and (3) the matrix formation of remodeling phase. Thus, skin wound healing is a finely controlled biological process involving a series of complex cellular interactions. Laser therapy is being implemented with increasing frequency in medicine. Low intensity laser is one that is capable of producing an energy density so low that any biologic alterations are the result of direct irradiation effect, not thermal events. This study was designed to evaluate the efficacy of low intensity laser therapy on skin wound healing in rabbits. A total of 10 male rabbits (New Zealand White Rabbit), age 8 weeks were used. Skin wound were surgically created dorso-lateral on the flank of 10 rabbits (2$\times$2 cm/damage areas). The experimental animals were treated with 5Hz (830 nm wave length) low-intensity laser (MILTA-01 Model) daily for 10 min (1.6 J/$cm^2$) for 12 days. Control animals were sham treated with the laser head. Laser irradiation animals showed a complete remodeling of the epithelial layer, a positive repair of connective tissues, and enhanced the wound closure rate over time as compared to the control animals. Especially, laser irradiation groups improved fibroblast activity, cellular content, granulation tissue formation, and collagen deposition which is resulted in improving the tensile strength of the wound. These findings suggest that laser photostimulation could accelerate healing of open wound in rabbits, and may be benefit in the treatment of open wound, including decubitis ulcers.

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Analyzing a Physical Marker to Identify Irradiated Dried Garlic and Cabbage (건마늘과 건양배추의 방사선 조사여부 확인을 위한 물리적 마커 분석)

  • Kim, Dong-Gil;Ahn, Jae-Jun;Jin, Qiong-Wen;Lee, Ho-Cheon;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.41 no.2
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    • pp.136-140
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    • 2009
  • The verification of irradiation treatments, using dried garlic and cabbage treated at 0-20 kGy, was investigated by analyzing the photostimulated luminescence (PSL), electron spin resonance (ESR) and thermoluminescence (TL) characteristics of the samples. The PSL results showed that the photon counts/60 sec of the non-irradiated dried garlic and cabbage were 287-337, corresponding to negative, while those of the irradiated samples were 7511-54063 photon counts/ 60 sec, corresponding to positive, making it possible to discriminate the non-irradiated from the irradiated samples. In ESR analysis, the dried garlic irradiated at 20 kGy exhibited cellulose radicals, whereas the irradiated dried cabbage showed crystalline sugar-induced multi-component signals, which were not found in the non-irradiated samples. The ESR signal intensity significantly increased as the irradiation dose increase ($R^2$= 0.9369 - 0.9926). The TL glow curves of the irradiated samples appeared at a temperature interval of 150-250, which were significantly different from those of non-irradiated samples, showing a significant increase in TL signal intensity with irradiation dose ($R^2$= 0.9670 - 0.9768). To enhance the reliability of the results, the first glow curve ($TL_1$) was compared with the second glow curve ($TL_2$) obtained after a re-irradiation step at 1 kGy. The TL ratio ($TL_1/TL_2$) was in good agreement with the reported TL threshold values for both the non-irradiated (<0.1) and irradiated (> 0.1) samples.

The effect of 470nm LED Irradiation on skin injury of RAT (470nm LED 광이 RAT 피부결손에 미치는 효과)

  • Cheon, Min-Woo;Kim, Seong-Hwan;Park, Yong-Pil;Lee, Ho-Shik;Kim, Young-Pyo;Kim, Tae-Gon;Lee, Hee-Keb
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.416-417
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    • 2008
  • This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. We developed the 4channel light medical therapy apparatus for external injury cure using a LED light source. This equipment was fabricated by using microcontroller, and designed to enable us to control light irradiation timer, intensity and reservation. In this paper, the designed device was used to find out how 470nm LEDs light source affects the skin wound of RAT. In the experiment, $1cm_2$ wounds on the External injury of RAT were made. Light irradiation RAT and none light irradiation RAT divided, each RAT was irradiated one hour a day for 15 days. In result, compared with none light irradiation RAT, the lower incidence of inflammation and faster recovery was shown in light irradiation RAT.

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Toxicity Assessment and Decomposition Characteristics of Triclosan in an E-beam Irradiation Process (전자빔 공정을 적용한 Triclosan의 제거특성 및 독성평가)

  • Chang, Taebum;Chang, Soonwoong;Lee, Sijin;Cho, Ilhyoung
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.3
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    • pp.5-11
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    • 2012
  • The abjective of this study was to study the degradation behavior and acute toxicity assessment of Triclosan and acute toxicity under E-beam irradiation. The experiments were conducted to investigate the efficts of the degradation efficiency in the initial concentration of Triclosan and the irradiation capacity of E-beam and the degree of mineralization based on a change of scavenger gas. The biological toxicity test by using on green algae, Pseudokirchneriella Subcapitata was conducted to lead the reducing toxicity. Degradation efficiency of Triclosan was improved when E-beam irradiation intensity was higher. Additionally, the % of TOC removal in each Radical scavenger gas was increased as the follows orders: $N_2O$ > $O_2$ > $N_2$, The toxicity test showed that the toxicity effect after 4 days(96hrs) was decreased by increase of E-beam irradiation intensity.