• 한국방사선학회논문지
• /
• 제6권2호
• /
• pp.99-106
• /
• 2012

본 연구는 선형가속기의 고에너지 엑스선을 조사한 생쥐 간에 대한 백삼과 동충하초의 방사선방호효과를 연구하였다. ICR계 수컷생쥐 군에 7일 동안에 경구적으로 백삼(150 mg/kg/day)과 동충하초(200 mg/kg/day)를 각각 방사선조사 전에 투여했고 다른 생쥐 군에 5 Gy(1.01 Gy/min)의 방사선량으로 전신조사를 했고 대조군에 생리적 식염수 (0.1 ml)를 투여 한 후 간 조직에서 환원형 글루타치온(GSH)과 산화형 글루타치온(GSSG)의 함량을 각각 검사하였다. 그 결과 방사선조사군(Rad)보다 동충하초투여군(EF+Rad)과 백삼투여군(WG+Rad)에서 환원형 글루타치온 (GSH)함량이 유의성 있게 증가했으나 산화형 글루타치온(GSSG)의 함량은 유의성 있게 감소하였다. 총 환원형 글루타치온(total GSH)과 산화형 글루타치온(GSSG)의 함량 비율은 방사선조사군(Rad)보다 동충하초투여군(EF+Rad)과 백삼투여군(WG+Rad)에서 유의성 있게 감소하였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권10호
• /
• pp.5725-5730
• /
• 2013

Cancer patients often suffer from local tumor recurrence after radiation therapy. Cell cycling, an intricate sequence of events which guarantees high genomic fidelity, has been suggested to affect DNA damage responses and eventual radioresistant characteristics of cancer cells. Here, we established a radioresistant lung cancer cell line, A549R, by exposing the parental A549 cells to repeated ${\gamma}$-ray irradiation with a total dose of 60 Gy. The radiosensitivity of A549 and A549R was confirmed using colony formation assays. We then focused on examination of the cell cycle distribution between A549 and A549R and found that the proportion of cells in the radioresistant S phase increased, whereas that in the radiosensitive G1 phase decreased. When A549 and A549R cells were exposed to 4 Gy irradiation the total differences in cell cycle redistribution suggested that G2-M cell cycle arrest plays a predominant role in mediating radioresistance. In order to further explore the possible mechanisms behind the cell cycle related radioresistance, we examined the expression of Cdc25 proteins which orchestrate cell cycle transitions. The results showed that expression of Cdc25c increased accompanied by the decrease of Cdc25a and we proposed that the quantity of Cdc25c, rather than activated Cdc25c or Cdc25a, determines the radioresistance of cells.

• 한국식품영양과학회지
• /
• 제31권4호
• /
• pp.717-721
• /
• 2002

ESR spectroscopy를 이용하여 한약재의 방사선 조사여부를 확인하였다. 국내산 한약재 4종으로 칡, 당귀, 아가리쿠스, 황기를 0, 1, 5 및 10 kGy의 Co-60 감마선을 조사한 후, 이를 시료로 Bruker-EPR spectrometer를 이용하여 조사로 생성된 라디칼을 측정하였다. 실험결과 조사직후 감마선 조사된 한약재 4종은 ESR 특성 신호를 나타내어 비조사시료와 확실하게 구별할 수 있었다. 적용선량의 범위에서(1~10 kGy) 조사선량이 증가함에 따라 ESR 신호 크기는 직선적으로 증가하였으며, 선량의 증가에 따른 신호 크기의 증가를 나타낸 $R^2$값은 0.9428~0.9942의 높은 값을 나타내었다. 또, 이들 신호는 상온에서 2주까지는 감소하였으나 저장기간 후에도 관찰할 수 있었으므로 ESR spectroscopy를 이용한 한약재의 방사선 조사여부 판별은 6주간의 저장 후에도 측정 가능한 방법임을 알 수 있었다.

• 한국식품영양과학회지
• /
• 제31권4호
• /
• pp.599-603
• /
• 2002

건조된 홍합을 방사선 조사시켜 생성된 hydrocarbon류를 분리하기 위하여 Soxtec기기를 이용하여 지방을 추출한 뒤, florisil column chromatography하였으며, GC/MS 분석기기로 성분을 확인하였다 0.5~10 kGy의 선량별로 조사된 건조된 홍합에서 방사선 조사에 의해 생성된 hydrocarbon류 함량이 조사선량에 따라 증가하였으며, 동일한 선량으로 조사된 시료임에도 방사선 조사에 의해 생성된 hydrocarbon류 각각의 함량이 다르게 정량되었다. 건조된 홍합의 방사선 조사에 의해 생성되는 hydrocarbon류와 2-alkylcyclobutanone류는 0.5 kGy 이상 조사된 시료에서 모두 검출되었으며, 비조사 시료에서는 검출되지 않았다.

• 대한수의학회지
• /
• 제41권1호
• /
• pp.105-111
• /
• 2001

We performed this study to determine the effect of Jiegeng(Platycodon grandiflorum), Danggui(Angelica sinensis), Gancao(Glycyrrhiza glabla), Chaihu(Bupleurum falcatnosa), Shoudehuang(Rehmannia glutinosa), Huangqi(Satragalus membranaceus), Muxiang(Saussurea lappa), Yuanzhi(Polygala tenuifolia), Rensen(Panax ginseng) and Baishaoyao(Paeonia lactiflolia), as Oriental radices herbs, on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of ${\gamma}-radiation$. Jiegeng(p<0.005), Danggui(p<0.0005), Gancao(p<0.005), Chaihu(p<0.05), Muxiang(p<0.05), Rensen(p<0.005) and Baishaoyao(p<0.005) were effective in intestinal crypt survival. Danggui(p<0.05), Chaihu(p<0.05), Shoudehuang(p<0.05), Huangqi(p<0.05), Rensan(p<0.005) and Baishaoyao(p<0.05) increased the formation of endogenous spleen colony. The frequency of radiation induced apoptosis was also reduced by pretreatment with Chaihu(p<0.05), Muxiang(p<0.005), Yuanzhi(p<0.05), Rensan(p<0.05) and Baishaoyao(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Danggui, Chaihu, Muxiang, Rensan and Baishaiyao might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• 대한두경부종양학회지
• /
• 제3권1호
• /
• pp.25-35
• /
• 1987

Irradiated 88 patients of Maxillary Sinus Carcinoma at Yonsei Cancer Center for 10years between 1971 and 1980 were retrospectively analysed. The majority of patients had very advanced disease(87.5% of $T_3\;and\;T_4$) and 17% of cervical lymph node involvement. 80.6% of all patients were epidermoid type. In 44 cases(50%), irradiation alone was performed. 28 cases(32%) of postoperative radiation after incomplete surgery and the remaining 16 cases(18%) of postoperative radiation after radical surgery were done. The majority of patients except 6 cases had irradiation a dose between 60Gy and 80Gy in 30-40 fractions over 6-8 weeks. The actuarial overall 3 and 5 year survival rate were 362% and 26%, respectively. The actuarial 5 year survival rate for 44 cases of radiation alone group was 14.1%, The actuarial 5 year survival rate for 28 cases of incomplete surgery and postoperative radiation group and 16 cases of radical surgery and postoperative radiation group were 312% and 67.4% respectively. In the actuarial 5 year survival rate according to the stage, stage II, III and IV were 79.5%, 20.9% and 0%. In recent, for the improvement of survival rate of advanced Maxillary Sinus Carcinoma at Yonsei Cancer Center, combined multidisplinary or trimodal treatment modality have been applied and in near time the more excellent results expect to be analyse.

• 한국대기환경학회지
• /
• 제22권5호
• /
• pp.724-730
• /
• 2006

A study on the electron beam(E-Beam) decompositions of volatile organic compounds(VOCs) under background gases(helium, nitrogen, oxygen, air) has been carried out. Hexane selected as aliphatic VOCs was irradiated with electron beam using a batch reactor in the dose range of $0{\sim}10kGy$ and at ambient temperature. The concentrations of Hexane were 140 ppmC, 300 ppmC and 600 ppmC, respectively. Irradiation treatment efficiency of Hexane was evaluated by the GC/FID, GC/MSD, and the by-products were analyzed by $CO/CO_2\;and\;O_3$ analyzers. In different concentration, the maximum degrees of Hexane decomposition at 10 kGy were 96.4%(140 ppmC), 88.3%(300 ppmC), and 49.9%(600 ppmC). It was found that the decomposition efficiency of Hexane was higher than that of toluene at the same conditions(140 ppmC). In addition, the formation of acetone, benzene, 2-hexanone, and 3-hexanone were observed after the treatment of Hexane.

• 폴리머
• /
• 제35권1호
• /
• pp.13-16
• /
• 2011

본 연구에서는 생체 적합성이 우수한 합성고분자 폴리비닐피롤리돈(PVP)과 천연고분자 카피-카라기난(${\kappa}C$), 1,2-헥산디올(HD)을 혼합하여 감마선 조사에 의한 방사선 가교로 하이드로젤을 제조하였다. 방사선 조사량은 25 kGy, ${\kappa}C$의 농도는 3 wt%로 고정하였다. 이렇게 제조된 하이드로젤의 의 PVP, HD의 농도에 따른 물리적 특성을 관찰하였다. PVP의 농도가 증가할수록 피롤리돈 분자 사이의 가교반응으로 젤화율과 연장강도는 증가하였고, 팽윤도는 감소하였다. 반면에 HD의 농도가 증가할수록 젤화율과 인장강도는 감소하였으며, 팽윤도는 증가하였다. 항균성 실험을 통해 HD를 함유한 하이드로젤에서 항곰팡이성 활성이 관찰되었다.

• 대한화학회지
• /
• 제16권2호
• /
• pp.80-83
• /
• 1972

The activity change of lysozyme resulted from its exposure to $Ti-H_2O_2$system in aqueous liquid at room temperature and to ${\gamma}$-irradiation in ice at $195^{\circ}K$ has been measured at room temperature with a Cary-14 spectrophotometer. The enzymatic activity of lysozyme which had been added to a previously flow-mixed solution of $TiCl_3$ and $H_2O_2$ (System I) was compared with the activity of a lysozyme-$H_2O_2$ solution after flow-mixing with $TiCl_3$ (System II), considering the differences between these two activity changes as the extent of the enzymatic inactivation by the involvement of OH radical reaction. The fraction of lysozyme inactivated by OH radical in the system containing 0.0025 M $TiCl_3-0.1M$ $H_2O_2$ (ph 3.5) was 13%, When the $TiCl_3$ concentration is double (pH 3.0), the fraction of enzyme inactivated increases to 36%. The activity of the system containing 0.025 M $TiCl_3-0.1$ M $H_2O_2$ (pH 1.5) was essentially zero. The results seem to support the previos view that the production of OH radical should be proportional to $TiCl_3$ concentration when $H_2O_2$ is present in excess. Increase in the extent of inactivation found in system I with increasing $TiCl_3$ concentration may be due to a pH effect. $H_2O_2$ seems to be less effective than $TiCl_3$ in the inactivation. 1% lysozyme solution, when ${\gamma}$-irradiated with a total dose of 3M rads, loses about 20% of its activity. Lowering of temperature also was found to yield a reduction in enzymatic activity.

• 한국식품과학회지
• /
• 제28권3호
• /
• pp.558-565
• /
• 1996

감마선 조사된 대투의 이화학적 품질 특성에서 일반성분 지방산 조성 및 무기질 성분은 모든 조사선량에서 변화가 없었으며, 질소 용해도파 추출성 phents 및 phytate 함량은 5 kGy까지의 조사군 사이에는 차이가 없었으나. 10 kGy와 20 k0y의 고선량 조사군에서는 다소의 영향이 화인되었으며, 1년 저장후에는 모든 시험군에서 감소현상을 보였다. 산가 및 유기산 함량도 2.5 kGy 조사군까지는 비조사군과 차이가 없었으나 5kGy 이상 조사군에서는 증가하는 경향이었고, 1년 저장 후 모든 시험군에서 증가함을 보였다. 조사된 대두의 총 아미노산 함량과 조성은 10 kGy 선량까지는 변화가 없었으나, 10kGy 이상의 조사군에서 cysteine과 methionine의 감소를 나타내었다. 대두 색도는 감마선 조사선량의 증가와 저장기간의 경과로 명도와 황색도의 감소가 적색도의 증가를 나타내었다.