• Title/Summary/Keyword: Ipomoea batatas L.

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Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato (자색고구마 캘러스배양에서 안토시아닌 생합성에 미치는 광의 영향)

  • Park Hyae-Jeong;Kim Jung-Suk;Park Hyeon-Yong
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.307-311
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    • 2005
  • The anthocyanin biosynthesis in callus culture of purple sweet potato (Ipomoea batatas L. cv. Borami) was investigated under growth in different light intensity and light emitting diodes (LED) treatment. Pigmented calli were induced from leaf explants cultured on MS agar medium supplemented with $0.5\;{\mu}M$ 2,4-D under light condition. The color value of these calli extracted after $2{\sim}4$ weeks of cultures was $0.4{\sim}0.5\;mg/mL$. Irradiation intensity is an important factor for anthocyanin biosynthesis. The optimal anthocyanin accumulation occurred on light intensity of 3000 lux. Light irradiation of 3000 lux and blue light treatment for 2 h resulted in a significant enhancement of anthocyanin accumulation. This value was 1.4 fold that the control.

Effects of Growth Regulators on Plant Regeneration in Shoot-Tip-Derived Embryogenic Callus Cultures of Sweet Potato (Ipomoea batatas) (고구마 경단 유래 배발생 캘러스로부터 식물체 재분화에 미치는 생장조절제의 영향)

  • ;Shozo FUJIOKA
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.5
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    • pp.281-286
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    • 1994
  • The hormonal regulation of organ differentiation was investigated in the tissue culture of sweet potato. Embryogenic callus was induced from shoot tips cultured on MS medium supplemented with 1 mg/L 2,4-D. When embryogenic callus was transferred to medium containing 0.1 mg/L GA$_4$, it proliferation was stimulated. The callus gave rise to plantlets when cultured on medium containing 0.1 mg/L BA. Addition of 0.1 mg/L jasmonic acid or 0.01 mg/L brassinolide to medium was effective for the development of healthy normal plantlets.

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Effect of Virus-free Plant and Subsoiling Reversion Soil for Reduction of Injury by Continuous Cropping of Sweet Potato (고구마 연작장해 경감을 위한 바이러스 무병묘 재배와 심토반전 효과)

  • Song, Hae-Ahn;Kim, Kab-Cheol;Lee, Seung-Yeob
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.254-261
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    • 2012
  • To reduce the injury by continuous cropping of sweet potato (Ipomoea batatas (L.) Lam.), the farmer's plant and virus-free plant were cultivated with the density of $70{\times}25cm$ (June 10, 2011) in continuous cropping soil (CCS) and subsoiling reversion soil (SRS). Fertilizer was applied at the rates of 55-63-156 $kg\;ha^{-1}$ ($N-P_2O_5-K_2O$) and 10 $ton\;ha^{-1}$ of cattle manure in CCS, and it was applied the 50% increased cattle manure compost and nitrogen in DRS. Symptoms of viral infection were revealed in the farmer's plant at 30 days after planting, but there were no symptoms in virus-free plant. The yield of virus-free plant was more increased 15% and 10.5% than that of farmer's plant in DRS and CCS, respectively. The yield of sweetpotato in SRS was more increased 8.8% and 3.2% in farmer's plant and virus-free plant compared to CCS, respectively. In DRS, the rate of marketable tuber of virus-free plant was increased by 80% compared to the farmer's plant (60.1%). The virus-free plant was produced the tuber with more brilliant peel color and well-formed shape compared to the farmer's plant. The increased yield of virus-free plant and in SRS soil condition showed a positive relationship (p=0.05) with the number of leaf per plant at 30 days and the number of branch per plant at 120 days after planting. The results showed that the early growth after planting was very important for the development of storage root. Therefore, the deep-subsoil reversion and cultivation of virus-free plant could be reduced the injury by continuous cropping of sweet potato, and increased farm income.

Growth Characteristics and Yield of Sweet Potato Cultivars between Virus-free and Farmer's Slips in Late Season Cultivation (고구마 바이러스 무병묘와 농가묘의 만기재배에서 품종 간 생육 및 수량특성)

  • Yoo, Kyoung-Ran;Lee, Seung-Yeob
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.1
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    • pp.43-49
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    • 2013
  • This work was conducted to obtain some information about stable production of high quality seed-tubers in the late season cultivation of virus-free sweet potato [Ipomoea batatas (L.) Lam.]. Growth characteristics and storage root yield between virus-free and farmer's slips in 9 cultivars were investigated using black-film vinyl mulching cultivation with $75{\times}25cm$ planting density on July 10. At 30 days after planting, vine length, vine diameter, number of node, and number of branch in virus-free slips were significantly increased than those in farmer's slips. The vine growth was significantly different among cultivars, and vine elongation was excellent in 'Kogeonmi', 'Shincheonmi', 'Shinhwangmi', 'Shinyulmi', and 'Yeonhwangmi' compared to the other cultivars. At 110 days after planting, vine length, vine diameter, number of node, number of branch, and fresh weight were significantly different among cultivars, but no significant differences between virus-free and farmer's slips were seen except number of node. Total yield in virus-free slips was increased by 12-49% among cultivars than that in farmer's slips. The mean yields between virus-free and farmer's slips were 1,625 kg/10a and 1,230 kg/10a, respectively, and it was significantly different between virus-free and farmer's slips. Percentage of marketable storage root in virus-free slips was 65.6%, and it was significantly higher than 57.8% in farmer's slips. Marketable yields ($40g{\leq}$) between virus-free and farmer's slips were 1,067 kg/10a and 710 kg/10a, respectively. Marketable yield in 'Shincheonmi', 'Shinyulmi' and 'Shinzami' was more than 1,300 kg/10a, and these cultivars showed to be highly adaptable for the late-season cultivation among 9 tested cultivars.

Growth-Promoting Effects of Vegetable Extracts on Selected Human Lactic Acid Bacteria

  • Kim, Moo-Key;Kim, Byung-Su;Baek, Bong-Rea;Shin, Dong-Hwa;Lee, Hoi-Seon
    • Preventive Nutrition and Food Science
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    • v.6 no.3
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    • pp.192-196
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    • 2001
  • Ethanol extracts from 36 vegetable samples were assayed for their growth-promoting effects on Bifidobacterium bifidum, B. longum, and Lactobacillus casei The growth-promoting effects varied according to bacterial strain and vegetable species. In modified Gy rgy broth, extracts of Lactuca sativa, Lycopersicon esculentum and L escutentum var. cerasiforme exhibited strong growth-promoting responses toward B. longum, and significant and strong growth- promoting response toward B. bifidum was observed in extracts of Actinidia arguta, Allium cepa, A. sativum, Brassica campestris subsp. napus vats. pekinensis, Capsicum frutescens, Daucus carota var. sativa, L sativa, 1. esculentum and L. esculentum var. cerasforme, Nelumbo nucifera, Cucurbita moschata, Lackca sativa var. capitata, and Rubus coreanus. For L casei, extracts of A. fshlosum, A. hberosum, Cichorium intbus, Cucurbita moschat\ulcorner Ipomoea batatas, 1. sativa var. capitata, L. esculentum, P. brachycarpa, Raphanus sativus, R. coreanus, and S. melongena strongly enhanced the growth of this bacteria. In modified Gy rgy broth, the promoting effect was most pronounced with B. bifidum and L. casei among lactic acid bacteria used. In MRS broth, A. arguta, A. cepa, A. sativum, B. campestris subsp. napus var. pekinensis, C. frutescens, and D. carota var. sativa L. satiw var. capitata, and R. coreanus strongly enhanced the growth of B. bifidum, Growth of B. longum was strongly affected by the addition of extracts from L. sativa var. capitata. For L casei, moderate growth-promoting responses were observed in 9 vegetable extracts. The promoting effect in MRS broth was most pronounced with B. bifidum among lactic acid bacteria used.

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Effect of Certain Additives on Bread-Making Quality of Wheat-Purple Sweet Potato Flours (밀 및 자색고구마 가루의 제빵성에 대한 첨가제의 영향)

  • 김선영;유정희
    • Korean journal of food and cookery science
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    • v.13 no.4
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    • pp.492-499
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    • 1997
  • The effect of oxidants, gluten and gums on breadmaking quality of wheat-purple sweet potato(Ipomoea batatas) composite flour were studied. Alveograph tests of doughs showed that all additives increased the P, L and W values. Of these additives, L-Ascorbic acid, gluten, carboxy methyl cellulose have a significant effect on alveogram indexes. SEM showed that the dough added with additives changed some what in appearance when compared with the control. When oxidants was added, the doughs discontinuous gluten film were observed. No significant differences ,were evident in bread crumb color among the additives. And textural characteristics of bread crumb were influened by adding additives. Breads containing additives showed an increase in max weight, strength and hardness and a decrease in springness and cohesiveness. Average enthalpy values for all bread crumb after 7days increased with storage time. However, addition of additives decrease the rate of staling slightly more than that of the control. Addition of L-Ascorbic acid, L-Cystine, carboxy methyl cellulose and hydroxy propyl methyl cellulose increased the loaf volume significantly, and with except potassium bromate bread scores were acceptable.

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Effect of Casein on Somatic Embryogenesis and Plant Regeneration in Shoot Apical Meristem Explants of Sweetpotato (Ipomoea batatas L.) (고구마 정단분열조직으로부터 체세포배발생 및 식물체 재분화에 미치는 casein의 영향)

  • Shin, Kong-Sik;Roh, Kyung-Hee;Lee, Yeon-Hee;Park, Young-Whan;Suh, Seok-Cheol
    • Journal of Plant Biotechnology
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    • v.31 no.1
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    • pp.67-72
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    • 2004
  • An efficient protocol has been developed for rapid mass propagation of sweetpotato from shoot-tips derived embryogenic callus. Optimal embryogenic callus was induced from shoot apical meristem explants on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4-D. The addition of casein hydrolysate in the media increased the embryogenesis efficiency of sweetpotato. Somatic embryos were easily induced from the embryogenic callus on MS basal medium containing 300-500mg/L casein hydrolysate without phytohormon. Treatment of casein hydrolysate (100∼300mg/L) with 1mg/L 2,4-D also improved the secondary embryonic efficiency from somatic embryos below 2mm in length. Plant regeneration was achieved via somatic embryogenesis and direct organogenesis. Regenerated planlets with well developed shoots and roots on MS basal medium were successfully transferred to soil.

Multiple Shoot Formation by Apical Meristem Culture in Ipomoea batatas Poir. (고구마 정단분열 조직배양에 의한 多芽體 형성)

  • 은종선;김영선
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.2
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    • pp.85-91
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    • 1999
  • In sweet potato cultivars 'Mokpo #29' and 'Sanchunza', shoots from extplants were formed 100% on the MS medium with 0.1 ㎎/L NAA and 2.0 ㎎/L BA after 30 days of culture and roots produced from the base of stem at frequencies of 66.7% ('Mokpo #29') and 69.2% ('Sanchunza'), respectively, The media with 0.5∼4.0 ㎎/L BA were produced the greatest frequency of multiple shoot and the most of shoots developed rapidly into normal plantlets with rooting within 60 days of culture. Whereas the cultivar 'Keumsi' failed to produce normal shoot multiplication on the medium with cytokinins alone because of callusing of adventitious shoots. When single shoots with 1 to 2 nodes were excised from the multiple shoot or shoots covered with callus devoid of root and transferred to MS medium with 4.0 ㎎/L BA or kinetin. Host divided shoots showed the callus induction at the stem base and it was enable to obtain regenerated plantlets with shoot and root normally.

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Effect of the Dextrose Equivalent of Maltodextrin on the Quality Characteristics of Jeju Purple Sweet Potato (Ipomoea batatas L.) during Molecular Press Dehydration (제주산 자색고구마의 분자압축탈수 중 말토덱스트린 Dextrose Equivalent의 영향에 대한 연구)

  • Cho, Man Jae;Kim, Hyun Jung
    • Korean Journal of Food Science and Technology
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    • v.47 no.6
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    • pp.744-750
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    • 2015
  • Purple sweet potatoes were dehydrated with maltodextrin with different dextrose equivalent (DE) values of 4-7, 13-17, 16.5-19.5, and 17-20. Maltodextrin was used as a molecular press dehydrating agent. The molecular dehydration rate of the purple sweet potatoes increased over time. As the DE of maltodextrin increased, the moisture content after 12 h of dehydration decreased from 65.7% to 40.8, 36.1, 34.9, and 28.6% for DE values of 4-7, 13-17, 16.5-17.5, and 17-20, respectively. Additionally, total phenolic content, anthocyanin, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities decreased as the DE value of maltodextrin increased. While maltodextrins with DE values of 16.5-19.5 and 17-20 effectively dehydrated the purple sweet potatoes, total phenolic, anthocyanin, and DPPH radical scavenging activities were lowered during dehydration. The DPPH radical scavenging activities correlated to both the total phenolic content ($r^2=0.96$) and anthocyanin contents ($r^2=0.95$) of the purple sweet potatoes. These results indicate that the purple sweet potatoes were effectively dehydrated with maltodextrin whose DE values ranged 16.5-20, although there were losses in the total phenolic and anthocyanin contents.

생명공학 분야의 "제2회 과학기술 예측" 조사 분석

  • 함경수
    • The Microorganisms and Industry
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    • v.25 no.2
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    • pp.11-18
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    • 1999
  • The sweet potatoes infected with witches'-broom disease were collected in the growing fields in Jeon-bug area, Korea. As a possible control plant, Ipomoea batatas L.var. Suwon 147 was selected. The pathogen was identified by various methods ; such as mechanical transmission, antibiotic reactions and electron microscopy. In the results attained the author believed the pathogen of the sweet potato infected with witches' broom to be a mycoplasma-like organism. the results are as follows : 1. Mycoplasma-like bodies were occurred in the phloem region of the sweet potatoes infected with witches'-broom and its particles were sized in the range of about 200-2,500m.mu.. The membrane of the pathogen was observed to be made of an unit. 2. Responsibilities to the antibiotices were sensitive in case of tetracycline and terramycin, and root dipping method showed remarkable than foliage spray. 3. The infection was developed by the grafting transmission but by the insects, Myzus persicae and Cicadella viridis. 4. rosette, witches'-broom, stunt, yellowish, mosaic and necrosis were observed as the symptomps of the disease.

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