• 대한임상검사과학회지
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• 제38권1호
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• pp.34-37
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• 2006

Beta-hemolytic streptococci (BHS) are frequently isolated pathogens in clinical microbiology laboratories. Among them, Group C, G and F streptococci infrequently cause respiratory infections, but they often invade other sites. Patients with underlying diseases are prone to be infected by the organisms and some of them can be fatal. Therefore, combination tests of serological and biochemical tests are needed to correct the identification of non-A, B streptococci because to various species belong to this species. The aim of this study was to investigate the isolation rates of strains and distribution of serogroup C, G and F streptococci at Wonju Hospital during the period of 2003-2004. 133 clinical isolates of group C, G and F streptococci were examined. Of them, 13 (9.8%) were group C, 41 (31.8%) were group F, 79 (59.4%) were group G. The prevalent isolation of the strains by serogroup were: group C, Streptococcus constellatus (38.5%); group F, Streptococcus anginosus (56.1%), Streptococcus constellatus (26.9%); group G, Streptococcus anginosus (54.4%), Streptococcus dysgalatiae subsp. equisimilis (26.5%). Overall, the most frequently isolated BHS was Streptococcus anginosus (51.2%).

• 생태와환경
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• 제47권4호
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• pp.239-246
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• 2014

2014년에 시행된 생물다양성 보전 및 이용에 관한 법률에 따라 외래생물의 사전예방적 관리 제도가 마련되었다. 아직 국내에 도입되지 않았으나 도입될 경우 생태계에 침입하여 부정적인 영향을 줄 수 있는 외래생물 중 포유류, 조류, 어류, 연체동물, 곤충, 식물을 포함한 24종이 위해우려종으로 지정되었다. 위해우려종을 국내로 수입 반입하고자 할 경우에는 환경부장관으로부터 승인을 받아야 한다. 이러한 제도는 기존의 법 체계에 비해 사전관리가 도입되었다는 측면에서 발전한 것이지만, 몇가지 개선해야 할 점이 있다. 첫째, 위해우려종의 범주 확대이다. 현재의 국내에 도입이 되지 않은 외래생물 대상에서, 국내에 도입되어 수족관, 식물원, 동물원 등의 사육 또는 재배시설 안에 제한적으로 존재하며 아직 생태계에는 정착되지 않은 외래생물까지 고려할 필요가 있다. 둘째, 정부에서는 수입 반입 신청자를 위하여 위해 우려종의 생태계위해성심사 세부 기준과 위해우려종이 자연 환경으로 탈출하는 것을 방지할 수 있는 안전관리 지침을 제공하여야 한다. 셋째, 위해우려종의 탈출에 대한 조기 탐지 및 신속 대응 방법 및 프로토콜이 개발되어야 한다.

• 한국수산과학회지
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• 제20권4호
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• pp.317-327
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• 1987

틸라피아(Tilapia mossambica)에 대한 간흡충의 제2중간정도로서의 역할 여부를 구명하기 위한 일환으로 우선 간흡충 유미유충의 틸라피아 치어에 대한 인공감염실험을 수행하여 그 역할을 할 수 없다는 사실을 천명한 다음 그 방어기전을 규명하기 위하여 그 체표면 점액으로부터 살충성 물질을 순수분리하여 기기분석을 통하여 그 화학적 구조를 확인한 결과를 요약하면 다음과 같다. 1. 입체현미경하에서 틸라피아 치어를 유미유충에 조우를 시도한 바 일반적으로 유미유충은 수분 후에 미부가 체부로부터 분리하기 시작하여 시간이 경과함에 따라 그 수가 증가하여 10여분 후에는 $80\%$에 이르렀으나 극히 소수의 유미유충은 어체표면에 부착한 채 체부만이 어체내로 침입하고 미부는 분리되었다. 2. 틸라피아 치어를 실내수조에서 유미유충에 24시간 노출시킨 바 일부 유미유충은 어체내로 침입하였으나 그 대부분이 피낭한 극소수도 4.2 시간 이내에 거의 모두 사감하였다. 3. 틸라피아의 체표면 점액의 에테르 추출물을 여러 단계의 화학적 처리를 거쳐 제3단계에서 분획한 유백색 상청액을 박막크로마토그라휘하여 얻은 2개의 반점물질을 간흡충의 탈낭유충에 in vitro에서 직접 접촉시켜 살충시험을 수행한 바 그 중에서 제1반점인 Rf. 0.2966 값의 물질이 다른 것에 비교하여 살충성의 월등하게 강력하였다. 4. 틸라피아의 체표면 점액의 에테르 유출물 66.50g으로부터 138mg의 살충성 순수물질이 회수되었으며, 그 수율은 $0.2075\%$, 그 정제도는 71배이었다. 5. 순수하게 분리된 살충성물질에 대하여 적외선, 자외선 및 핵자기공명 분광분석을 이용하여 구조확인을 하였던 바 이 물질은 linoleic acid로 판명되였다. 이상의 실험결과로 미루어 보아 틸라피아는 자연계에 있어서 간흡충의 제2중간숙주 역할을 할 수 없으며, 그 체표면 정액내의 살충성물질인 linoleic acid가 그 방제기전에 관여하고 있다고 생각한다.

• 대한수의학회지
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• 제13권2호
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• pp.147-160
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• 1973

The present experiments proposed to pass judgement upon the suitability as the second intermediate host of Clonorchis sinensis, of three kinds of fresh-water fish by having them infected with the cercaria of Clonorchis sinensis and then observing the penetrating ability of the cercaria, and maturity, the process of degeneration and extinction, and infectivity of the metacercaria of Clonorchis sinensis. The following is a brief summary of the leading facts gained through the experiments; 1. P. parva was easily subject to invasion of the cercaria, A. signifer was much less subject to the invasion, and finally A. asmussi was hard to invade. And the infectivity of the cercariae was in proportion to their penetrating ability mentioned in the above. 2. The examination of the cercariae after having three kinds of fresh-water fish infected with them disclosed that 24 hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, and that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and they made a vigorous rotary movement intermittently. 3. Then the metacercariae came to a state of maturity and beyond this stage some metacercariae in P. parva started the process of degeneration and extinction in 133 days; some in A. asmussi, in 140 days; and A. signifer, in 70 days. As more days elapsed, their degeneration and extinction increased in number, and in 269 days all of them in A. signifer became dead while those in A. asmussi were all dead in 460 days. However almost all of them in P. parva survived even after 770 days. The results shown above revealed that P. parva was the most suitable as the second intermediate host among three kinds of fresh-water fish and that A. signifer and A. asmussi were not quite recommendable as the second intermediate host. The ability of the cercaria to invade fresh-water fish, and life span of the metacercaria within fresh-water fish vary outstandingly according to species of fresh-water fish. An explanation as to the mechanism must wait as the subject to be further pursued.

• 대한미생물학회지
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• 제34권5호
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• pp.435-443
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• 1999

Orientia tsutsugamushi is obligate intracellular bacterium that grows within the cytoplasm of the eukaryotic host cells. Therefore capability of the attachment, entry into the host cell and intracellular survival should be critical process for oriential infection. In this study we investigated the cellular invasion mechanism of Orientia tsutsugamushi and the role of transmembrane heparan sulfate proteoglycan, which binds diverse components at the cellular microenvironment and is implicated as host cell receptors for a variety of microbial pathogens. First of all Orientia tsutsugamushi can invade a wide range of nonprofessional phagocytic cells including fibroblast, epithelial cells and endothelial cells of various host species, including Band T lymphocytes. Thus, it was postulated that the attachment of O. tsutsugamushi requires the recognition of ubiquitous surface structures of many kinds of host cells. Treatments with heparan sulfate and heparin inhibited the infection of Orientia tsutsugamushi in dose-dependent manner for L cell, mouse fibroblast, whereas other glycosaminoglycans such as chondroitin sulfate had no effect. Collectively, these findings provide strong evidence that initial interaction with heparan sulfate proteoglycan is required for the oriential invasion into host cells.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.238-244
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• 2010

Rhizobia are well-known for their ability to infect and nodulate legume roots, forming a nitrogen-fixing symbiosis of agricultural importance. In addition, recent studies have shown that rhizobia can colonize roots and aerial plant tissues of rice as a model plant of the Graminaceae family. Here we show that rhizobia can invade tobacco, a model plant belonging to the Solanaceae family. Inoculation of seedling. roots with five GFP-tagged rhizobial species followed by microscopy and viable plating analyses indicated their colonization of the surface and interior of the whole vegetative plant. Blockage of ascending epiphytic migration by coating the hypocotyls with Vaseline showed that the endophytic rhizobia can exit the leaf interior through stomata and colonize the external phyllosphere habitat. These studies indicate rhizobia can colonize both below- and above-ground tissues of tobacco using a dynamic invasion process that involves both epiphytic and endophytic lifestyles.

• 대한수의학회지
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• 제8권2호
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• pp.98-109
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• 1968

The small type piroplasma which is known to be prevailing in Korean cattle was injected to un-susceptible hosts such as mice, guinea pigs, rats, rabbits, fowls and developing chicken eggs by i.v., i.p, and intra C.A.M. routes, and its morphological development in each animal species was observed. 1. The small type piroplasma of cattle did not produce any pathogenicity in the experimental animals such as mice, guinea pigs, rats, rabbits, fowls and developing chicken embryos. However, only a partial sign for developing cycle of the organism was observed in the peritoneal fluid. The schizogony of protozoa was not detected in any organs of the inoculated animals. 2. In the animals inoculated, the parasites of I-III types, relative to the process of time in the host, have changed their shapes into IV-VI types and then VI-X types however, the type X was not restored to the type I. In guinea pigs, more grape type was detected then X type in number, and in process of time, the shape was changed from round to elliptic, and the pointed end of the organism appeared in flesh color. 3. None of the organisms were found from the blood stream of inoculated animals excepts only a few piroplasma as were found in the experimental mice shortly after inoculation or i.v. route, No protozoa was also found in the organs of the animals autopsied. 4. The form change of protozoa was more clear in these animals than that in susceptible host. The most piroplasmas have changed their forms into VI-X types 30 hours after inoculation and were dispersed from R.B.C. Tracing of piroplasrna beyond this stage was not attempted. 5. The small type piroplasma comprises in I. X types and it is further believed that VII-X types which were freed from one cell proceeded to invade others. The from of development resembles to the small type of piroplasma which is prevailing in Japan.

• 대한지리학회지
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• 제36권4호
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• pp.444-457
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• 2001

우리 나라 대나무의 시.공간적 분포에 영향을 미친 요인을 분석하였다. 참대속부터 산죽속에 이르는 모든 대나무의 분포 북한계선은 백령도 - 장수산 - 멸악산 - 용문산 - 명지산 - 추애산 - 후탕 - 묘향산 - 명천에 이르는 선 남쪽이다. 화석에 따르면 한반도의 대나무는 지질시대부터 분포하였고, 고문헌에 의하면 대나무는 조선시대에 의식주 등의 생활재료로 널리 사용되었다. 조선 초기부터 일제 때까지의 고문헌들을 기초로 한 왕대와 이대 등 군현별 시대적 분포지 복원에 따르면 대나무는 시대에 따라 분포지가 변화하였다. 대나무의 시.공간적 분포역 변화는 기후변화 등 자연적 요인과 함께 재배와 이식 등 인위적인 영향에 의한 것이다. 최근 국내 죽세산업이 침체되면서 대 나무숲이 다른 용도로 바뀌어 사라지거나, 반대로 방치되어 확산되면서 자연식생을 교란시키므로 농촌경관의 유지를 위하여 관리와 보전 대책이 요구된다.

• 한국응용곤충학회지
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• 제47권1호
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• pp.107-110
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• 2008

Tropical fire ant로 알려진 Solenopsis geminata (Fabricius)는 열대 및 온대지역에 분포하는 잡식성 개미로서, 식물에 피해를 주기도 하지만 야외활동을 하는 사람들이 물릴 경우 심하게 부어오르고 통증을 유발하는 위생해충으로 유명하며 우리나라에 침입 시 제주도 및 남부지방의 농경지 및 산림에 피해를 줄 것으로 예상되어 현재 식물방역법상 관리해충으로 지정된 종이다. 이 개미는 선박을 통하여 항구주위에서 먼저 정착하여 확산되고 있으며, 현재 동남아, 중국남부지역에서 급격히 확산되고 있다. 우리나라에서는 1990년대에는 수입식물검역 과정에서 검출실적이 1건이었으나 2000년부터 검출빈도가 증가추세에 있어 2006년도에는 7건이 검출되어 국내 침입이 우려되고 있다. 따라서, 동종에 대한 형태, 분포, 기주식물, 생태 등에 대한 자료를 정리하여 금후 수입식물검역시 참고하고 국내 침입시 조기 박멸하기 위한 자료로 이용하고자 한다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.260-260
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• 2014

Dermatophytes can invade in keratinized tissues and cause dermatophytosis [1] that rank among the most widespread and common infectious diseases world-wide. Although several systemically and topically administered drugs with activities against these fungi are available, still complete eradication of some of these infections, is difficult and relapses and remissions are often observed [2,3]. In addition, some people are allergic to many of the available drugs which add complications even more. Therefore, the search for novel, selective and more effective therapy is always required and it may help the clinicians to choose the correct treatment for their patients. Non-thermal plasmas primarily generate reactive species and recently have emerged as an efficient tool for medical applications including sterilization. In this study, we evaluated the ability of non-thermal dielectric barrier discharge (DBD) plasma for the inactivation of clinical isolates of Trichophyton genera, Trichophyton mentagrophytes (T. mentagrophytes) and Trichophyton rubrum (T. rubrum), which cause infections of nails and skin and, are two of the most frequently isolated dermatophytes [4]. Our results showed that DBD plasma has considerable time dependent inactivation potential on both T. mentagrophytes and T. rubrum in-vitro. Furthermore, the mechanisms for plasma based T. mentagrophytes and T. rubrum inactivation and planning for in-vivo future studies will be discussed.