• 대한치과보철학회지
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• 제60권2호
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• pp.168-174
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• 2022

상악 전치부의 임플란트 식립 시 치은 퇴축이나 골 결손 문제를 동반하는 경우에는 심미적인 임상 결과를 얻기가 쉽지 않다. 본 증례에서는 상악 우측 중절치에서 순측 치조골판의 소실이 진단되어 발치 후 연조직을 확보한 후에 골 이식을 동반하는 임플란트 식립을 계획하였다. 또한 이상적인 임플란트 식립 위치를 위해 디지털 가이드 수술을 시행하였고, 치조골 결손부가 광범위하기 때문에 하악지에서 자가골 채취 후 이종골과 함께 골유도재생술을 동반하였다. 충분한 임플란트의 골 유착 기간을 거친 뒤 2차 수술 및 인상 채득을 통한 임시 보철물을 제작하였고, 주기적인 외형 조정을 통해 연조직의 형태를 개선하였다. 최종 보철물 제작시에는 양극 처리를 시행한 맞춤형 지대주를 사용하여 자연 치아의 색조를 유도하였고, 구강 스캔을 통하여 임시 보철물의 형태를 재현해 줌으로써 심미적이고 기능적인 지르코니아 보철물을 장착해 주었다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권6호
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• pp.511-519
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• 2006

Autogenous bone grafts have been considered the gold standard for maxillofacial bony defects. However, this procedure could entail a complicated surgical procedure as well as potential donor site morbidity. Possibly the best solution for bone-defect regeneration is a tissue engineering approach, i.e. the use of a combination of a suitable scaffold with osteogenic cells. A major source of osteogenic cells is the bone marrow. Bone marrow-derived mesenchymal stem cells are multipotent and have the ability to differentiate into osteoblastic, chondrocytic, and adipocytic lineage cells. However, the isolation of cells from bone marrow has someproblems when used in clinical setting. Bone marrow aspiration is sometimes potentially more invasive and painful procedure and carries of a risk of morbidity and infection. A minimally invasive, easily accessible alternative would be cells derived from periosteum. The periosteum also contains multipotent cells that have the potential to differentiate into osteoblasts and chondrocytes. In the present study, we evaluated the osteogenic activity and mineralization of cultured human periosteal-derived cells. Periosteal explants were harvested from mandibule during surgical extraction of lower impacted third molar. The periosteal cells were cultured in the osteogenic inductive medium consisting of DMEM supplemented with 10% fetal calf serum, 50g/ml L-ascorbic acid 2-phosphate, 10 nmol dexamethasone and 10 mM -glycerophosphate for 42 days. Periosteal-derived cells showed positive alkaline phosphatase (ALP) staining during 42 days of culture period. The formation of ALP stain showed its maximal manifestation at day 14 of culture period, then decreased in intensity during the culture period. ALP mRNA expression increased up to day 14 with a decrease thereafter. Osteocalcin mRNA expression appeared at day 7 in culture, after that its expression continuously increased in a time-dependent manner up to the entire duration of culture. Von Kossa-positive mineralization nodules were first present at day 14 in culture followed by an increased number of positive nodules during the entire duration of the culture period. In conclusion, our study showed that cultured human periosteal-derived cells differentiated into active osteoblastic cells that were involved in synthesis of bone matrix and the subsequent mineralization of the matrix. As the periosteal-derived cells, easily harvested from intraoral procedure such as surgical extraction of impacted third molar, has the excellent potential of osteogenic capacity, tissue-engineered bone using periosteal-derived cells could be the best choice in reconstruction of maxillofacial bony defects.