• The Korean Journal of Mycology
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• v.40 no.4
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• pp.187-190
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• 2012

Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizo-pus oryzae, Lichtheimia corymbifera.

• Mycobiology
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• v.44 no.1
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• pp.29-37
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• 2016

During a biodiversity survey of undiscovered taxa in Korea, two zygomycetous fungal strains were isolated. The first strain, EML-FSDY6-1 was isolated from a soil sample collected at Dokdo Island in the East Sea of Korea in 2013, and the second strain, EML-DG-NH3-1 was isolated from a rat dung sample collected at Chonnam National University garden, Gwangju, Korea in 2014. Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer, 18S and 28S rDNA, actin and translation elongation $factor-1{\alpha}$ genes. EML-FSDY6-1 and EML-DG-NH3-1 isolates were confirmed as zygomycete species, Absidia pseudocylindrospora and Absidia glauca, respectively. Neither species has previously been described in Korea.

• Mycobiology
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• v.42 no.4
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• pp.391-396
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• 2014

In the past two decades, European ash trees (Fraxinus spp.) have been severely damaged due to ash dieback disease, which is caused by the fungal species Hymenoscyphus fraxineus (Chalara fraxinea in the anamorphic stage). Recent molecular phylogenetic and population genetic studies have suggested that this fungus has been introduced from Asia to Europe. During a fungal survey in Korea, H. fraxineus-like apothecia were collected from fallen leaves, rachises, and petioles of Korean ash and Manchurian ash trees. The morphological and ecological traits of these materials are described with the internal transcribed spacer rDNA sequence comparison of H. fraxineus strains collected from Korea, China and Japan.

• Mycobiology
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• v.43 no.4
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• pp.402-407
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• 2015

Two new fungal species of the genus Talaromyces, Talaromyces purpurogenus and Talaromyces trachyspermus from the Trichocomaceae family, were recovered during an investigation of fungal communities in soil collected from the Gangwon-do and Jeollanam-do provinces of Korea. These two species have not been previously officially reported from Korea. In this study, detailed descriptions of internal transcribed spacer rDNA and beta-tubulin gene regions of these two fungi are presented. Morphological features of the two fungi in five agar media, potato dextrose, oatmeal, malt extract, czapek yeast extract, and yeast extract sucrose, are also reported. The species were identified on the basis of molecular and morphological analysis, and herein we present data with detailed descriptions and figures.

• Mycobiology
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• v.43 no.4
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• pp.392-401
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• 2015

Using dilution plating method, 47 fungal isolates were obtained from a soil sample collected from Dokdo in the East Sea of Korea in 2013. In this study, two fungal isolates, EML-MFS30-1 and EML-DDSF4, were confirmed as undescribed species, Metarhizium guizhouense and Mortierella oligospora in Korea based on current classification system using multi loci including rDNA internal transcribed spacer, large subunit, small subunit, and ${\beta}$-tubulin (BTUB) genes. Herein, detailed morphological descriptions on characters of the undescribed fungal species as well as their molecular phylogenetic status are provided with comparisons to related species.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.87-93
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• 2020

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 ㎛ (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.

• Mycobiology
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• v.44 no.4
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• pp.191-201
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• 2016

In this study, the phylogeny and morphology of Mycosphaerella nawae (Dothideomycetes, Ascomycota) were examined using Korean and Japanese isolates, to establish the phylogenetic relationship between M. nawae and its allied species. Korean and Japanese isolates of M. nawae were collected from circular leaf spot-diseased leaves and were confirmed based on internal transcribed spacer (ITS) sequence data. Phylogenetic analysis was conducted using multiple genes, including the ITS region, 28S rDNA, ${\beta}-tubulin$, translation elongation $factor-1{\alpha}$, and actin genes. Our results revealed that M. nawae is closely related to members of the genus Phaeophleospora but are distant from the Ramularia spp. In addition, microscopic analysis revealed pseudothecia on the adaxial and abaxial surface of overwintered diseased leaves (ODL) and only on the abaxial surface of diseased leaves. Ascospores are oval to fusiform, one-septate, tapered at both ends, $1.7{\sim}3.1{\times}8.1{\sim}14.1{\mu}m$, and were observed in ODL. Conidia are oval, guttulate, one-septate, $3.5{\sim}4.9{\times}12.8{\sim}19.8{\mu}m$, and barely discernable on 30-day cultures. To our knowledge, this is the first report on the phylogeny of M. nawae, which is closely related to the genus Phaeophleospora, especially P. scytalidii.

• Mycobiology
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• v.45 no.4
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• pp.312-317
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• 2017

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

• Mycobiology
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• v.45 no.4
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• pp.344-352
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• 2017

During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.

• The Plant Pathology Journal
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• v.27 no.1
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• pp.85-88
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• 2011

Podosphaera fusca (syn. Sphaerotheca fusca, S. fuliginea) is a causal agent of powdery mildew infections on Zinnia elegans, and has been documented in Korea since 1967. An outbreak involving about 1,000 plants in October 2008, in Yangpyeong, Korea, was caused by Golovinomyces cichoracearum. Further surveys of zinnia powdery mildew from 2008-2010 confirmed the widespread occurrence of Zinnia-Golovinomyces association in Korea. Chasmothecia of this fungus have not been found on Z. elegans in Korea. Analysis of internal transcribed spacer rDNA from five Korean collections confirmed the powdery mildew as G. cichoracearum. Comparison with the those from GenBank revealed 100% identity with sequences from zinnia powdery mildew, forming a monophyletic clade with 100% bootstrap support, and with those of G. cichoracearum parasitic on the hosts belonging to the tribe Heliantheae of the family Asteraceae.