• Title/Summary/Keyword: Internal transcribed spacer rDNA

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A New Record of Neosartorya aureola Isolated from Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.43 no.3
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    • pp.191-195
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    • 2015
  • A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures.

First Report of Two Colletotrichum Species Associated with Bitter Rot on Apple Fruit in Korea - C. fructicola and C. siamense

  • Park, Myung Soo;Kim, Byung-Ryun;Park, In-Hee;Hahm, Soo-Sang
    • Mycobiology
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    • v.46 no.2
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    • pp.154-158
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    • 2018
  • Bitter rot caused by the fungal genus Colletotrichum is a well-known, common disease of apple and causes significant yield loss. In 2013, six fungal strains were isolated from Fuji apple fruits exhibiting symptoms of bitter rot from Andong, Korea. These strains were identified as Colletotrichum fructicola and C. siamense based on morphological characteristics and multilocus sequence analysis of the internal transcribed spacer rDNA, actin, calmodulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase Pathogenicity tests confirmed the involvement of C. fructicola and C. siamense in the development of disease symptoms on apple fruits. This is the first report of C. fructicola and C. siamense causing bitter rot on apple fruit in Korea.

First Report on Fusarium Wilt of Zucchini Caused by Fusarium oxysporum, in Korea

  • Choi, In-Young;Kim, Ju-Hee;Lee, Wang-Hyu;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
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    • v.43 no.2
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    • pp.174-178
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    • 2015
  • Fusarium wilt of zucchini in Jeonju, Korea, was first noticed in May 2013. Symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. Infected plants eventually died during growth. Based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rDNA and translation elongation factor $1{\alpha}$), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates. To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

A New Record of Gongronella butleri Isolated in Korea

  • Babu, A. Giridhar;Kim, Sang Woo;Adhikari, Mahesh;Yadav, Dil Raj;Um, Yong Hyun;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.43 no.2
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    • pp.166-169
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    • 2015
  • We report the isolation of a Gongronella butleri species and describe it based on the analysis of the internal transcribed spacer region of rDNA and morphological characteristics. G. butleri has been reported as a high chitosan producer in the literature. This is the first record of G. butleri isolated from crop field soil in Korea.

Mycoflora and Enzymatic Characterization of Fungal Isolates in Commercial Meju, Starter for a Korean Traditional Fermented Soybean Product

  • Baek, Jin-Ho;So, Kum-Kang;Ko, Yo-Han;Kim, Jung-Mi;Kim, Dae-Hyuk
    • Mycobiology
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    • v.42 no.3
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    • pp.291-295
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    • 2014
  • Mycoflora was assessed in the commercial meju from four well-separated geographic origins. A total of 112 fungal isolates were identified by phenotypic characteristics and molecular taxonomy using sequencing the internal transcribed spacer of the rDNA and revealed 19 species from 13 genera. Enzymatic characteristics of protease and amylase, and mycotoxin production were analyzed.

Sporothrix stylites : A New Record from Field Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Back, Chang-Gi;Kang, In-Kyu;Ten, Leonid;Lee, Hyang Burm;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.3
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    • pp.224-228
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    • 2017
  • The fungal strain, designated KNU16-008, was isolated from field soil in Chungcheongnam-do, Korea. The isolated fungi was characterized by morphological and phylogenetic analyses. Isolated fungus showed typical morphological characteristics of the genus Sporothrix. Based on its phylogenic analysis using internal transcribed spacer (ITS) of rDNA and ${\beta}$-tubulin gene sequences, the strain KNU16-008 was identified as Sporothrix stylites. This species has not been previously reported in Korea.

First Report of Leaf Spot of Datura metel Caused by Alternaria tenuissima in Korea

  • Aktaruzzaman, Md.;Kim, Joon-Young;Afroz, Tania;Kim, Byung-Sup
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.330-333
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    • 2015
  • In June 2013, we collected leaf spot disease samples of Datura metel from Gangneung, Gangwon Province, Korea. The symptoms observed were small circular to oval dark brown spots with irregular in shape or remained circular with concentric rings. We isolated the pathogen from infected leaves and cultured the fungus on potato dextrose agar. We examined the fungus morphologically and confirmed its pathogenicity according to Koch's postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4), glycerol-3-phosphate dehydrogenase (G3PDH) and the RNA polymerase II second largest subunit (RPB2) gene sequence revealed that the causal agent was Alternaria tenuissima. To the best of our knowledge, this is the first report of leaf spot of D. metel caused by A. tenuissima in Korea as well as worldwide.

A New Record of Volutella ciliata Isolated from Crop Field Soil in Korea

  • Babu, Anam Giridhar;Kim, Sang Woo;Yadav, Dil Raj;Adhikari, Mahesh;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.43 no.1
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    • pp.71-74
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    • 2015
  • During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.

First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax

  • Deng, Jian Xin;Kim, Chang-Sun;Oh, Eun-Sung;Yu, Seung-Hun
    • Mycobiology
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    • v.38 no.4
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    • pp.316-320
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    • 2010
  • Leaf spot and blight disease was observed on two-year-old seedlings of Dendropanax morbifera (Korean name: Hwangchil tree) during July of 2008 in Jindo Island, Korea. Symptoms included yellow-brown to dark brown irregularly enlarged spots frequently located along the veins of leaves. The lesions were often surrounded by chlorotic haloes. Severe leaf blight and subsequent defoliation occurred when conditions favored disease outbreak. The causal organism of the disease was identified as Alternaria panax based on morphological characteristics and sequence analysis of the internal transcribed spacer region of rDNA. A. panax isolates induced leaf spots and blight symptoms not only on D. morbifera but also on the other members of Araliaceae tested. This is the first report of foliar blight caused by A. panax on D. morbifera.

First Report of Post-Harvest Fruit Rot of Aronia melanocarpa Caused by Fusarium tricinctum in Korea

  • Lee, Hye Won;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.1
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    • pp.68-71
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    • 2016
  • Black chokeberry, Aronia melanocarpa (Michx.) Elliott, is commonly used as a source of jam and jelly in Korea and worldwide. A fungal isolate EML-CCB6 was isolated from the decaying fruit of black chokeberry. Based on the morphological characteristics and rDNA internal transcribed spacer sequence analysis, the isolate was identified as Fusarium tricinctum (Corda) Sacc. This is the first report of post-harvest fruit rot of black chokeberry caused by F. tricinctum in Korea.