• 제목/요약/키워드: Internal transcribed spacer

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ITS 서열에 의한 한국 왕대속 식물종의 계통분류학적 연구 (Phylogenic Study of Genus Phyllostachys (Phyllostachys) in Korea by Internal Transcribed Spacer Sequence (ITS))

  • 이송진;허만규;허홍욱;이병룡
    • 생명과학회지
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    • 제21권9호
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    • pp.1281-1287
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    • 2011
  • 왕대속은 벼과 또는 화본과에 속하는 다년생 목본성 초본이며 아시아의 인도와 중국에 많은 종이 주로 분포한다. 식물에서 속간 속내 계통관계 추론을 위해 널리 이용되고 있는 ITS 부위가 있다. 이 속에 속하는 우리나라 자생 식물 왕대 속내 네 식물종 간 계통 관계를 평가하기 위해 핵 게놈의 ITS 부위로 평가하였다. DNA 서열 배당은 많은 갭(gaps)을 가지고 있었다. 이 속 내 서열 변이는 일부 삽입과 결실이 발견되었고 속내 종간 변이는 자연도태에 의한 것으로 밝혀졌다. 이 속의 오죽과 분죽은 세 계통도(MP, ML, and NJ)에서 모두 같은 분지군을 형성하여 가장 근연관계에 있었다. 기존의 형태학적 특성과 단순 반복 서열(ISSR)의 결과와 유사한 계통 관계를 나타내어 왕대속에서 ITS의 서열이 이들 분류군에 매우 유익한 정보를 제공하고 있음을 시사하였다.

Taxonomy of $Ganoderma$ $lucidum$ from Korea Based on rDNA and Partial ${\beta}$-Tubulin Gene Sequence Analysis

  • Park, Young-Jin;Kwon, O-Chul;Son, Eun-Suk;Yoon, Dae-Eun;Han, Woo-Ri-Ja-Rang;Yoo, Young-Bok;Lee, Chang-Soo
    • Mycobiology
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    • 제40권1호
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    • pp.71-75
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    • 2012
  • In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial ${\beta}$-tubulin gene sequence of the $Ganoderma$ species. The size of the ITS rDNA regions from different $Ganoderma$ species varied from 625 to 673 bp, and those of the partial ${\beta}$-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which revealed that Korean $Ganoderma$ $lucidum$ strains belong in a single group along with a $G.$ $lucidum$ strain from Bangladesh.

Prevalence and Phylogenetic Analyses of Trichuris suis in Pigs in Hunan Province, Subtropical China

  • Tan, Lei;Wang, Aibing;Yi, Jing;Liu, Yisong;Li, Jiayu;Liu, Wei
    • Parasites, Hosts and Diseases
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    • 제56권5호
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    • pp.495-500
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    • 2018
  • Trichuris suis infection in pigs is ubiquitous in intensive and extensive farms, which causes potential threat to human health. The objective of this research was to investigate the prevalence of T. suis in pigs in Hunan province. Total 2,267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions (Hunan province) were evaluated for the existence of T. suis eggs using saturated NaCl floating method. The average infection rate of T. suis in pigs was 8.91% in Hunan province. To determine genetic variation of the gained T. suis isolates in the present study, the internal transcribed spacer (ITS) regions from nuclear ribosomal DNA (rDNA) of 7 T. suis isolates were cloned and analyzed. Nucleotide diversities were 1.0-3.5% and 0-3.8% for ITS-1 and ITS-2, respectively. Phylogenetic analyses indicated that all isolates collected in the present study and T. suis available in Genbank generated a monophyletic clade. The present investigation revealed high infection rates of T. suis in pigs in Hunan province, which shed light on making effective measures to prevent and control T. suis infection in pigs in Hunan province.

DNA Profiles of Trichoderma spp. in Korea

  • Park, Dong-Suk;Kang, Hee-Wan;Park, Young-Jin;Lee, Mi-Hee;Lee, Byoung-Moo;Hahn, Jang-Ho;Go, Seung-Joo
    • Mycobiology
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    • 제32권1호
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    • pp.24-34
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    • 2004
  • Molecular approaches, internal transcribed spacer(ITS) sequences of ribosomal DNA, and Universal Rice Primer Polymerase Chain Reaction(URP-PCR) were used to investigate the genetic diversity, taxonomic complexity, and relationships of Trichoderma species in mushroom farms. Forty-one isolates of 13 Trichoderma spp. were used in this study and clustered into eight groups. The DNA fingerprint patterns and ITS1 region sequence alignment data showed similar results, but not in some species, such as T. virens, T. atroviride, T. harzianum, and T. aureoviride. Results of this study have proven that the morphology-based taxonomic system has some limitations in terms of classification. The data obtained in this study would be a good index for classifying indistinguishable Trichoderma strains.