• YAKHAK HOEJI
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• v.54 no.4
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• pp.295-299
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• 2010

The aim of this study was to develop and validate for determination of alibendol in human plasma by HPLC method. After precipitation of 500 ${\mu}l$ plasma samples by 50% methanol 50 ${\mu}l$ and 60% perchloric acid 30 ${\mu}l$ and the supernatant 50 ${\mu}l$ was injected into HPLC. The assay was performed isocratically using 10 mM potassium phosphate (pH 3.0) and acetonitrile (80 : 20, v/v) as mobile phase. The $C_{18}$ column (particle size $3.5{\mu}m$, $4.6{\times}50$ mm, Zorbax Eclipse) was used as a solid phase. The mobile phase was delivered at a flow-rate of 1.7 ml/min, detection was by ultraviolet absorption at 232 nm and concentrations were calculated on the basis of peak areas. In these conditions, alibendol can be separated from ethylparaben, the internal standard, and endogenous substances. The retention times of alibendol and ethylparaben were just about 2.6 and 3.5 minutes, respectively. This rapid HPLC method was validated by examining the precision and accuracy for inter- and intra-day analysis. The standard curve was linear ($R^2$=1.0000) over the concentration range of 0.05~20 ${\mu}g$/ml. The inter-day relative standard deviation (R.S.D.) and accuracy were 0.2~12.2% and 94.4~101.2% (82.7% at the lower limit of quatitation). The intra-day R.S.D. and accuracy were 0.1~11.8% and 98.8~102.5%, respectively. The method was successfully applied to the determination of alibendol in plasma for a pharmacokinetic study.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.7
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• pp.652-659
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• 2011

A computational system to predict flowfield and infrared signature in aircraft exhaust system is developed. As the first step, a virtual mission profile is considered and an engine is selected through a performance analysis. Then a nozzle that meets the requirement of each mission is designed. The internal flow in the exhaustion nozzle at the maximum thrust is analyzed using a state-of-the-art CFD code. In addition, a system to combine information of the skin temperature distribution of the nozzle and after-body surface with an infrared prediction code is developed. Finally, qualitative results for the infrared signature reduction design are obtained by investigating the infrared signature level under various conditions.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.5
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• pp.1145-1158
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• 1994

A three-dimensional, finite difference, numerical model with free surface was developed on ${\sigma}$-coordinate. A semi-implicit numerical scheme in time has been adopted for computational efficiency. The scheme is essentially independent of the stringent stability criteria (CFL condition) for explicit schemes of external surface gravity wave. Implicit algorithm was applied for vertical shear stress, Coriolis force and pressure gradient terms. The reliability of the model with vertically variable grid system was checked by the comparison of simulation results with analytic solution of wind-driven currents in a one-dimensional channel. Sensitivity analysis of differencing parameters was carried out by applying the model to the calculation of wind-driven currents in a square lake.

• Journal of Korean Society of Water and Wastewater
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• v.25 no.6
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• pp.825-837
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• 2011

Seonakdong river consists of stagnant sections whose flowrate is controlled by the Daejeo and Noksan gates. As a result, there is not a minimum flow during normal times. The Daejeo and Noksan gates are located at the upstream head and the downstream end of Seonakdong river, respectively. Seonakdong river is an estuarine tributary of Nakdong river, which is a reservoir-like river used for agricultural irrigation, with the gate at the estuary of the river to prevent the intrusion of saline. Since the construction of the water gates, the water quality of the river has become degraded. This could also be due to the internal loading of pollutants, especially nutrients, from the sediments of the river because of the elongated detention time by the water gates. This study was thus conducted for the purpose of evaluating the current hydrologic-cycle system and providing measures for the rehabilitation of the hydrologic cycle. In this research, the daily outflow in Seonakdong River was simulated using the SWAT and SWMM models, and the water quality concentration including BOD, SS, TN, and TP were analyzed. The possibility of the application of SWAT-SWMM hybrid simulation was determined through the verification of both models. The error analysis shows that the results of both SWAT and SWAT-SWMM simulations make good agreements with those of field observations. For the single simulation results of SWAT, $R^{2}$ and NSE are 0.758, 0.511, respectively. For the hybrid simulation results of SWAT-SWMM, those are 0.880, 0.452, which means that the hybrid simulation can give more accurate results for the watershed where both the agricultural and urban areas exist.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.8
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• pp.4685-4688
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• 2013

We aimed to investigate the mechanism and effects of autophagy on cisplatin (DDP)-induced apoptosis in human gastric cancer cell line SGC7901. After SGC7901 cells were treated with DDP and/or chloroquine, cell proliferation was measured using MTT assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by Western blot; and quantitative analysis of autophagy after monodansylcadaverine (MDC) staining was performed using fluorescence microscopy. We found after treatment with 5 mg/L DDP for 24 h, the rates of cell apoptosis were ($21.07{\pm}2.12$)%. Autophagy, characterized by an increase in the number of autophagic vesicles and the level of LC3-II protein was observed in cells treated with DDP. After inhibition of autophagy by chloroquine, the rates of cell apoptosis were increased to ($30.16{\pm}3.54$)%, and the level of Caspase-3 and P53 protein were increased, and Bcl-2 protein was decreased. Therefore, autophagy protects human gastric cancer cell line SGC7901 against DDP-induced apoptosis, inhibition of autophagy can promote apoptosis, and combination therapy with DDP and chloroquine may be a promising therapeutic strategy for gastric cancer.

• The Journal of the Korea Contents Association
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• v.5 no.5
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• pp.296-304
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• 2005

Quantitative analysis compare to dynamic characteristic change of the regional cerebral blood volume(rCBV) after development of cerebral fat embolism in cats using perfusion magnetic resonance(MR) Imaging. Twenty cats were used. Linoleic acid (n=11) were injected into the internal carotid artery using microcatheter through the transfemoral approach. Polyvinyl alcohol (Ivalon) (n=9) was injected as a control group. Perfusion MR images were obtained at 30 minutes and 2 hours after embolization, based on T2 and diffusion-weighted images. The data was time-to-signal intensity curve and ${\Delta}R_2^*$ curve were obtained continuously with the aid of home-maid image process algorithm and IDL(interactive data Banguage, USA) softwares. The ratios of rCBV increased significantly at 2 hours compared with those of 30 minutes (P<0.005). In conclusion, cerebral blood flow decreased in cerebral fat embolism immediately after embolization and recovered remarkably in time course. It is thought that clinically informations to dynamic characteristic change of the cerebral hemodynamics to the early finding in cerebral infarction by diffusion weighted imaging(DWI) and perfusion weighted imaging(PWI).

• Analytical Science and Technology
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• v.15 no.5
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• pp.410-416
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• 2002

In this study, a new quantitative analytical method has been developed for the rapid determination of vancomycin in human plasma and urine using liquid chromatography/tandem mass spectrometry (LC - MS/MS). Chromatography was carried out on a $C_{18}$ XTerra MS column ($2.1{\times}30mm$) with a particle size of $3.5{\mu}m$. The mobile phase was 0.25% formic acid in 10% acetonitrile and the flow rate was $250{\mu}L/min$. Vancomycin and caffeine (internal standard) were detected by MS/MS using multiple reaction monitoring (MRM). Vancomycin gives a predominant doubly protonated precursor molecule ($[M+2H]^{2+}$) at m/z 725.0 and a corresponding product ion of m/z 100.0. Detection of vancomycin was good, accurate and precise, with a limit of detection of 1 nM in plasma. The calibration curves for vancomycin in human plasma was linear in a concentration range of $0.01{\mu}M$ - $100{\mu}M$ for plasma. This method has been successfully applied to determine the concentration of vancomycin in human plasma and urine from pharmacokinetic study and relative studies.

• Journal of Energy Engineering
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• v.24 no.2
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• pp.72-78
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• 2015

In the present study, a recuperator to improve the thermal efficiency of a micro gas turbine is considered. The counter flow plate-fin heat exchanger with offset strip fins is chosen as the type of the recuperator. From the optimization study as varying design parameters of the recuperator determined from the ideal cycle analysis, the internal structure of the recuperator is determined. The recuperator is made from stainless steel 304. In order to evaluate performance of the recuperator, experimental investigation is performed. The effects of inlet temperature of hot-side of the recuperator on the thermal performance of the recuperator are investigated. As a result, effectiveness of the recuperator obtained from the experiments is well consistent with that obtained from the correlations.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.581-589
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• 2009

The water extract of Dohongsamul-tang(DHSMT) has been traditionally used in treatment of ischemic heart and brain diseases in Oriental Medicine. However, little is known about the mechanism by which DHSMT protects C6 glial cells from glucose deprevation induced damages. Therefore, this study was designed to evaluate the protective effects of DHSMT on 2-deoxy-D-glucose induced autophagy of C6 glial cells. Autophagic phenotype is evaluated by fluorescence microscopy and flow cytometry with specific biological staining dyes, including monodansylcadaverine and acridine orange, as well as Western blot analysis with microtubule-associated protein 1 light chain 3(LC3) and Beclin-1. Treatment with 2-deoxy-D-glucose significantly resulted in a decrease of the viability of C6 glial cells and increase of the extracellular LDH release in a dose and time-dependent manner. However, pretreatment with DHSMT protected C6 glial cells from glucose deprivation with 2-deoxy-D-glucose. The author also observed the fact that autophagy phenotype occurred by 2-deoxy-D-glucose in C6 glial cells. Pretreatment with 3-MA, a pharmacological inhibitior of autophagy, abolished the formation of acidic vesicle organelle in C6 glial cells treated with 2-deoxy-D-glucose. However, pretreatment with DHSMT inhibited the formation of autophagic phenotypes, including formation of acidic vesicle organelle, and increase of the expression of LC-3 II Beclin-1 proteins in C6 glial cells treated with 2-deoxy-D-glucose. Taken together, these data suggest that DHSMT is able to protect C6 glial cells from glucose deprivation with marked inhibition of autophagy formation.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.11
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• pp.1091-1097
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• 2017

Sales of commercial-type cooling fans intended for application in military electronics are often discontinued during equipment production. This results in requirements for alternative fan selection as well as equipment performance and reliability tests, such as high-temperature operation testing. This study deals with alternative fan selection and verification methods that can be used during the production process. First, an alternative fan was selected by calculating the flow and pressure required to effectively cool the equipment, then the feasibility of the selected fan was verified using a reliable CFD heat dissipation analysis model. Following this, a high-temperature operation test was performed using the alternative fan in the equipment. Results demonstrated that the equipment satisfied its required function in a high-temperature environment, and the main parts as well as internal air temperature were found to be thermally stable.