• Tuberculosis and Respiratory Diseases
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• v.40 no.1
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• pp.43-51
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• 1993

Background: The polymerase chain reaction (PCR) is a very sensitive method for the detecting of mycobacterial DNA. There are many reports revealing the efficacy of PCR for the diagnosis of M. tuberculosis, but there are many different methods for DNA extraction from Mycobacterium tuberculosis. Bead beater method is a very useful method for DNA extraction from clinical spectimens, but its procedures are relatively complicated and time-consuming. So we studied other methods for the DNA extraction from Mycobacterium tuberculosis $H_{37}Rv$ and some clinical specimens (5 smear positive sputa and 5 smear negative CSF). Method: We extracted the mycobacterial DNA with 6 different methods from H37Rv strain and clinical specimens. The methods included SDS-microwave oven method, NaOH lysis method, Triton X-100-Proteinase K method, Lysis buffer method, SDS-proteinase K method and bead beater method. The target DNA was 123bp of IS6110 and was detected by examination of ethidium bromide-stained agarose gels. Results: Among 6 methods, SDS-proteinase K method, bead beater method, lysis buffer method and triton X-100-proteinase K method were excellent, but SDS-proteinase K method was the best method in the aspect of simplicity and cost-effectiveness. Conclusion: We suggest that SDS-porteinase K method is a simple and convinient method and might be the best method for the extraction of mycobacterial DNA.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.1
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• pp.76-82
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• 2002

This paper presents a quaternary-binary decoder, a quaternary logic current buffer, and a quaternary logic full-adder using current-mode multiple-valued logic CMOS circuits. Proposed full-adder requires only 15 MOSFET, 60.5% and 48.3% decrease of devices are achieved compared with conventional binary CMOS full-adder and Current's full-adder. Therefore, decrease of area and internal nods are achieved. Designed circuits are simulated and verified by HSPICE. Proposed full-adder has 1.5 ns of propagation delay and 0.42㎽ of power consumption. Also, proposed full-adder can easily adapted to binary system by use of encoder, designed decoder and designed current buffer.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.11a
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• pp.473-476
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• 2003

This paper shows necessity of encapsulation layer to maximite flexibility of brittle indium-tin-oxide (ITO) on polymer substrates. And, Young's modulus (E) of encapsulation layer have an significant effect on external bending stress and the coefficient of thermal expansion (CTE) of that have a significant effect on internal thermal stress. To compare magnitude of total mechanical stress including both bending stress and thermal stress, the mechanical stress of triple-layer structure (substrate / ITO / encapsulation layer or substrate / buffer layer / ITO) can be quantified and numerically analyzed through the farthest cracked island position. As a result, it should be noted that multi-layer structures with more elastic encapsulation material have small mechanical stress compared to that of buffer and encapsulation structure of large Young's modulus material when they were externally bent.

• Korean Journal of Materials Research
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• v.29 no.2
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• pp.106-115
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• 2019

Recently, metal cases are widely used in smart phones for their luxurious color and texture. However, when a metal case is used, electric shock may occur during charging. Chip capacitors of various values are used to prevent the electric shock. However, chip capacitors are vulnerable to electrostatic discharge(ESD) generated by the human body, which often causes insulation breakdown during use. This breakdown can be eliminated with a high-voltage chip varistor over 340V, but when the varistor voltage is high, the capacitance is limited to about 2pF. If a chip capacitor with a high dielectric constant and a chip varistor with a high voltage can be combined, it is possible to obtain a new device capable of coping with electric shock and ESD with various capacitive values. Usually, varistors and capacitors differ in composition, which causes different shrinkage during co-firing, and therefore camber, internal crack, delamination and separation may occur after sintering. In addition, varistor characteristics may not be realized due to the diffusion of unwanted elements into the varistor during firing. Various elements are added to control shrinkage. In addition, a buffer layer is inserted in the middle of the varistor-capacitor junction to prevent diffusion during firing, thereby developing a co-fired product with desirable characteristics.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.2
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• pp.1-7
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• 2012

DDoS attack is a malicious attempt to exhaust resources of target system and network capacities using lots of distributed zombi systems. DDoS attack introduced in early 2000 has being evolved over time and presented in a various form of attacks. This paper proposes a scheme to detect DDoS attacks and to reduce possibility of such attacks that are especially based on vulnerabilities presented by using control packets of existing network protocols. To cope with DDoS attacks, the proposed scheme utilizes a buffer management techniques commonly used for congestion control in Internet. Our scheme is not intended to detect DDoS attacks perfectly but to minimize possibility of overloading of internal system and to mitigate possibility of attacks by discarding control packets at the time of detecting DDoS attacks. In addition, the detection module of our scheme can adapt dynamically to instantly increasing traffic unlike previously proposed schemes.

• Journal of Information Processing Systems
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• v.2 no.2
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• pp.76-81
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• 2006

A PDA is used mainly for downloading data from a stationary server such as a desktop PC in an infrastructure network based on wireless LAN. Thus, the overall performance depends heavily on the performance of such downloading with PDA. Unfortunately, for a PDA the time taken to receive data from a PC is longer than the time taken to send it by 53%. Thus, we measured and analyzed all possible factors that could cause the receiving time of a PDA to be delayed with a test bed system. There are crucial factors: the TCP window size, file access time of a PDA, and the inter-packet delay that affects the receiving time of a PDA. The window size of a PDA during the downstream is reduced dramatically to 686 bytes from 32,581 bytes. In addition, because flash memory is embedded into a PDA, writing data into the flash memory takes twice as long as reading the data from it. To alleviate these, we propose three distinct remedies: First, in order to keep the window size at a sender constant, both the size of a socket send buffer for a desktop PC and the size of a socket receive buffer for a PDA should be increased. Second, to shorten its internal file access time, the size of an application buffer implemented in an application should be doubled. Finally, the inter-packet delay of a PDA and a desktop PC at the application layer should be adjusted asymmetrically to lower the traffic bottleneck between these heterogeneous terminals.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.12
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• pp.913-917
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• 2009

Flash memories have asymmetric I/O costs for read and write in terms of latency and energy consumption. However, the ratio of these costs is dependent on the type of storage. Moreover, it is becoming more common to use two flash memories on a system as an internal memory and an external memory card. For this reason, buffer cache replacement algorithms should consider I/O costs of device as well as possibility of reference. This paper presents WWCLOCK(Write-Weighted CLOCK) algorithm which directly uses I/O costs of devices along with recency and frequency of cache blocks to selecting a victim to evict from the buffer cache. WWCLOCK can be used for wide range of storage devices with different I/O cost and for systems that are using two or more memory devices at the same time. In addition to this, it has low time and space complexity comparable to CLOCK algorithm. Trace-driven simulations show that the proposed algorithm reduces the total I/O time compared with LRU by 36.2% on average.

• The Korean Journal of Zoology
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• v.15 no.2
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• pp.71-85
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• 1972

This investigation was undertaken to establish the ultrastructural organization of the retina in domestic fowl (Gallus domesticus B.) comparing with the ultrastructure that has been indicated in other Aves by several workers. The electron microscope observations were made on selected segments of retinal tissue prefixed for 2 hrs in 1.25% glutaraldehyde buffered with 0.2 M cacodylate at pH 7.2 and then postfixed in cold 1% osmium tetroxide in 0.4 M cacodylate buffer for 2 hrs. After postfixation, tissues were dehydrated in alcohol series, embedded in Epon 812 mixture from propylene oxide and stained with saturated uranyl acetate and $Pb(NO_3)_2$ solution. Specimens were examined with a Hitachi HS-7S electron microscope. The pigment epithelia cells contain numerous mitochondria with prominent dense granules and several changeful spaped Golgi bodies. The internal fine structure of the receptor outer segments revealed the characteristic stacks or arrays of bimembranous disks. The ellipsoid outer portion of the cone inner segments is composed of a tightly packed mass of extraordinarily large mitochondria. The outer limiting membrane is seen to contain many junctional complexes, the fibrillar material of which is electron-dense.

• The Journal of Internal Korean Medicine
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• v.11 no.1
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• pp.165-177
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• 1990

In order to study the effects of HWA GAE SAN known clinically for their effects of treatment for cough and asthma, the study was carried out to investigate the effect of HWA GAE SAN extract on the contractile force of isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows. 1. Preparation of isolated guinea pig trachea smooth muscle was suspended in the oxygeneted Kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$ and recorded the developed tension by the drug with the isometric transducer (Nacro F-60). The restin tension was approximately 0.5g. 2. The trachea smooth muscle in normal state showed a significant atony to the increase of density of HWA GAE SAN. 3. The atonic effect of the trachea smooth muscle was restricted after prescription of Pyrilamine& Cyproheptadine. Hireceptor broker, which were prearranged. 4. After 5, 15, 50& $150{\mu}l/ml$ of HWA GAE SAN were prescribed, the atony of trachea smooth muscle was caused by Histamine. 5. After 50& $150{\mu}l/ml$ of HWA GAE SAN were prescribed, the atony of trachea smooth muscle was remarkably dwindled which was caused by Acetylcholine. 6. After $150{\mu}l/ml$ of HWA GAE SAN were prescribed, the atony of trachea smooth muscle was remarkably dwindled which was caused by 5-Hydroxytryptamine.

• The Journal of Internal Korean Medicine
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• v.11 no.1
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• pp.77-91
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• 1990

This study was carried out to investigate the effect of Jasoeumja extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows: 1. The isolated trachea smooth muscle was suspended in the Organ bath with oxygenated Kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer (Nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Jasoeumja extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by Jasoeumja extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was considerably inhibited by Jasoeumja extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydroxytryptamine $10^{-4}M$ was considerably inhibited by Jasoeumja extract.