• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.260-260
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• 1994

Mammalian pyruvate dehydrogenase complex(PDC) enzyme consists of multiple oopies of three major oligomeric enzymes-El, E2 E3. And protein X is one of the enzymatic constituents which is tightly bound to E2 subunit This complex enzyme is responsible for the oxidative decarboxylation of pyruvate producing of acetyl CoA which is a key intermediate for the entry of carbohydrates into the TCA cycle for its complete metabolic conversion to CO$_2$. And the overall activity of the complex enzyme is regulated via covalent nodification of El subunit by a El specific phosphatase ad kinase. Protein X has lipoyl moiety that undergoes reduction and acetylation during ezymatic reaction and has been known h be involved in the binding of E3 subunit to E2 core and in the regulatory activity of kinase. The purification of protein X has not been achieved majorly because of its tight binding to E2 subunit The E2-protein X subcomplex was obtained by the established methods and the detachment of protein X from E2 was accomplished in the 0.1M borate buffer containing 150mM NaCl. During the storage of the subcomplex in frozen state at -70$^{\circ}C$, the E2 subunit was precipitated and the dissociated protein X was obtained by cntrifegation into the supernatant The verification of protein X was accomplished by (1)the migration on SDS-PAGE, (2)acetylation by 〔2$\^$-l4/C〕 pyruvate, and (3)internal amino acid sequence analysis of tryptic digested enzyme.

• BMB Reports
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• 제39권1호
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• pp.105-110
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• 2006

We have screened 766 strains of fungi from the BIOTEC Culture Collection (BCC) for xylanases working in extreme pH and/or high temperature conditions, the so-called extreme xylanases. From a total number of 32 strains producing extreme xylanases, the strain BCC7928, identified by using the internal transcribed spacer (ITS) sequence of rRNA to be a Marasmius sp., was chosen for further characterization because of its high xylanolytic activity at temperature as high as $90^{\circ}C$. The crude enzyme possessed high thermostability and pH stability. Purification of this xylanase was carried out using an anion exchanger followed by hydrophobic interaction chromatography, yielding the enzyme with >90% homogeneity. The molecular mass of the enzyme was approximately 40 kDa. The purified enzyme retained broad working pH range of 4-8 and optimal temperature of $90^{\circ}C$. When using xylan from birchwood as substrate, it exhibits $K_m$ and $V_{max}$ values of $2.6{\pm}0.6\;mg/ml$ and $428{\pm}26\;U/mg$, respectively. The enzyme rapidly hydrolysed xylans from birchwood, beechwood, and exhibited lower activity on xylan from wheatbran, or celluloses from carboxymethylcellulose and Avicel. The purified enzyme was highly stable at temperature ranges from 50 to $70^{\circ}C$. It retained 84% of its maximal activity after incubation in standard buffer containing 1% xylan substrate at $70^{\circ}C$ for 3 h. This thermostable xylanase should therefore be useful for several industrial applications, such as agricultural, food and biofuel.

• Journal of Pharmaceutical Investigation
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• 제35권6호
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• pp.431-436
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• 2005

A high-performance liquid chromatographic method was validated for quantitation of nadolol in human plasma. Nadolol and internal standard, pindolol, were extracted with tert-butyl methyl ether after addition of 10 M sodium hydroxide solution. The analytes were separated on a reverse phased C18 column using a mobile phase consisting of 0.05 M ammonium phosphate monobasic buffer, acetonitrile and methanol (81: 17:2 v/v/v) and detected using a fluorescence detector (excitation wavelength 230 nm, emission wavelength 294 nm). The method was specific and sensitive enough to detect as low as 3 ng/mL of nadolol in human plasma. Linear calibration range was 3-150 ng/mL with correlation coefficient greater than 0.999. The overall accuracy was in the range of 96.8 to 103% and precision C.V.(%) 7.30 to 12.2%. The recovery was approximately 100% and stability was confirmed during storage and sample preparation. The present HPLC method was successfully applied to study bioavailability after oral administration of 80 mg of nadolol in healthy Korean subjects. The mean $AUC_{t}$ was $1968{\pm}397\;ng{\cdot}hr/mL$ and $C_{max}$ of $186{\pm}79.3\;ng/mL$ was reached at $3.5{\pm}0.76\;hr$. The mean $t_{1/2}$ of nadolol was $17.3{\pm}2.59\;hr$.

• Journal of Pharmaceutical Investigation
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• 제41권5호
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• pp.289-294
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• 2011

A rapid and specific high performance liquid chromatography-tandem mass (LC/MS/MS) method for the analysis of montelukast in human plasma has been developed and validated. After cold acetonitrile-induced precipitation of the plasma samples, montelukast and glipizide (internal standard, IS) were eluted on a reverse-phase $C_{18}$ column by isocratic mobile phase consisted of 10 mM ammonium formate buffer (adjusted to pH 3.5 with formic acid) and acetonitrile (3:97, v/v). Acquisition was performed with multiple reaction monitoring (MRM) mode by monitoring the transitions: m/z 587.2${\rightarrow}$ 423.2 for montelukast and m/z 446.0${\rightarrow}$321.2 for IS. Ranges of concentration for calibration curves (10-1000 ng/mL) showed correlation coefficients ($r^2$) were better than 0.9948. Precision of intra- and inter-day ranged from 3.70 to 11.68% and from 3.04 to 12.95%, accuracy of intra-day and inter-day ranged from 93.34 to 102.75% and from 100.79 to 107.63%, respectively. The described method provides a fast and sensitive analytical tool for determining montelukast levels in plasma, and was successfully applied to a pharmacokinetic study in 16 healthy human subjects after oral administration of 10mg tablet formulation of montelukast sodium under fasting conditions.

• 전자공학회논문지
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• 제51권6호
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• pp.124-131
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• 2014

본 논문은 무선 개인영역네트워크 환경에서 중계 프로토콜 기능을 가진 시분할다중접속방식(TDMA)의 적응형 기저대역 중계모뎀을 설계한 내용을 기술한다. 설계한 기저대역 중계모뎀은 마스터 동기 신호에 의해 제어되며 최대 14홉의 중계 네트워크를 구성할 수 있다. 효과적인 데이터 중계 통신을 위해 단일포트 메모리에서 우선권을 사용하여 내부 버퍼 설계를 최적화하였다. 그리고 메모리 버스 제어기는 합성된 게이터 수를 최소화시킬 수 방법으로 설계하였다. 협대역 TDMA 중계 통신의 동기 기능을 구현하기 위하여 네트워크 슬롯 동기회로와 프레임 동기회로를 분리하여 게이트수를 줄였다. 이 방법을 사용하여 9만 게이트의 Xilinx FPGA XC6SLX9에서 약 37%(34,000게이트)를 사용하였다. 32비트 싱크워드를 사용한 1024비트 프레임의 통신 수신율은 약 96.4%이다. 설계된 기저대역 중계모뎀을 사용하여 14홉의 중계에서 측정한 최대전송지연시간은 230.4ms이다.

• 전자공학회논문지CI
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• 제41권4호
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• pp.41-49
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• 2004

본 논문은 FPGA 임베디드 기가비트 트랜시버의 테스트에 관한 방법과 측정 결과를 다룬다. 실험에 사용한 디바이스는 Altera 사의 Stratix GX 디바이스로 범용 고속 프로토콜을 지원하는 트랜시버(GXB)이다. 본 논문은 차세대 IO 버스로 대두되는 PCI Express 직렬 프로토콜을 GXB에 구현하였다. PCI Express 규격에 맞게 생성된 GXB 모듈은 타이밍 시뮬레이션을 거쳐 하드웨어 구현과 테스트를 수행하였다. 트랜시버 테스트 방법으로 GXB 내부 블록 테스트, GXB 신호 무결성 테스트, GXB 입출력 버퍼 및 온칩 터미네이션 테스트, GXB 프로토콜 테스트의 네 가지 검증 절차를 거쳤다. 본 논문을 통해 FPGA 임베디드 트랜시버의 설계방법과 테스트 절차, 측정 결과를 제시한다.

• 대한전자공학회논문지SD
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• 제39권5호
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• pp.65-73
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• 2002

본 논문에서는 순차적 입력 데이터 처리방식을 이용하여 2048 point FFT/IFFT를 단일 칩으로 구현하는 방법을 제안하고 검증하였다. 순차적으로 입력되는 2028개의 복소 데이터를 처리하기 위해서는 입력 데이터를 저장하는 버퍼가 필요하고 이 입력 버퍼로는 DRAM 회로를 이용한 지연 변환기 (delay commutator)를 사용하여 전체 칩 면적을 35% 이상 줄일 수 있었다. 전체 FFT/IFFT는 16 point FFT를 기본 블록으로 사용하며, radix-4 구조를 가지는 다섯 단계와 radix-2 구조를 가지는 하나의 단계로 이루어져 있다. 각 단계마다 연산을 수행하면서 증가되는 결과 S/N 비를 유지하면서 비트 라운딩을 하기 위해 convergent block floating point (CBFP) 알고리즘을 적용하여 digital audio broadcasting(DAB)을 위한 단일 칩 설계에 기여하였다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2012년도 춘계학술대회 논문집
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• pp.521-526
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• 2012

It is essential for the accurate time-domain prediction of broadband noise due to turbulence-cascade interaction to develop inflow/outflow boundary conditions to satisfy the following three requirements: to maintain the back ground mean flow, to nonreflect the outgoing disturbances and to generate the specified input gust. The preceding study(1) showed that Perfectly Matched Layer (PML) boundary condition was successfully applied to absorb the outgoing disturbances and to generate the specified gust in the time-domain computations of broadband noise due to interaction of incident gust with a cascade of flat-plates. In present study, PML boundary condition is extended in order to predict steady mean flow that is needed for the computation of noise due to interaction of incident gust with a cascade of airfoils. PML boundary condition is originally designed to absorb flow disturbances superimposed on the steady meanflow in the buffer zone. However, the steady meanflow must be computed before PML boundary condition is applied on the flow computation. In the present paper, PML equations are extended by introducing source term to maintain desired mean flow conditions. The extended boundary condition is applied to the benchmark problem where the meanflow around a cascade of airfoils is predicted. These illustrative computations reveal that the extended PML equations can effectively provide and maintain the target meanflow.

• 한국소음진동공학회논문집
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• 제22권7호
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• pp.685-691
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• 2012

It is essential for the accurate time-domain prediction of broadband noise due to turbulence-cascade interaction to develop inflow/outflow boundary conditions to satisfy the following three requirements: to maintain the back ground mean flow, to nonreflect the outgoing disturbances and to generate the specified input gust. The preceding study showed that perfectly matched layer(PML) boundary condition was successfully applied to absorb the outgoing disturbances and to generate the specified gust in the time-domain computations of broadband noise due to interaction of incident gust with a cascade of flat-plates. In present study, PML boundary condition is extended in order to predict steady mean flow that is needed for the computation of noise due to interaction of incident gust with a cascade of airfoils. PML boundary condition is originally designed to absorb flow disturbances superimposed on the steady meanflow in the buffer zone. However, the steady meanflow must be computed before PML boundary condition is applied on the flow computation. In the present paper, PML equations are extended by introducing source term to maintain desired mean flow conditions. The extended boundary condition is applied to the benchmark problem where the meanflow around a cascade of airfoils is predicted. These illustrative computations reveal that the extended PML equations can effectively provide and maintain the target meanflow.

• 한국실내디자인학회논문집
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• 제20권4호
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• pp.74-82
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• 2011

All the phenomena and subjects of nature and society are within correlation interconnection, and they are inseparably connected one another. The elements of this interaction can be found out through the concept of transparency in the space composition of Korean traditional architecture. This study is focusing on the access space, in other words, a gate-house that is a buffer zone playing a process role up to the main space among successive spaces. It was chosen to be the subject of the study since it strengthens convergence into the main building and with the effect connecting spaces, it could show well the spatial possibility of transparency. Besides, the subject of the study is limited to the Moonru Multi roofs that improves the functionality of spaces between gate-houses, and it is intended to progress contents by comparative analysis of two kinds such as Seo Won gate-house and temple gate-house. Korean traditional architecture places emphasis on harmony within the whole spaces. There are intimate relations between surrounding environment, external spaces and internal spaces, and it is important understand the spatial relations according to the shape appearing through interactions of parts in the whole spaces. In conclusion, the Moonru Multi roofs is analyzed with the method of extracting the concept that is contained in the frame of analysis and through ecological views through a visible and structural method. It can be understood what kinds of method for communication were used for ancestors to recognize and use spaces with the deduced concept through the analysis of the Moonru Multi roofs with different character.