• 동의생리병리학회지
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• 제34권1호
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• pp.7-13
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• 2020

Sanyeoleumja (SY) is the traditional Korean medicinal prescription for the treatment of inflammatory diseases of eyes. In this study, the anti-inflammatory effects of SY water extract were investigated. To measure the anti-inflammatory effects of SY, we examined the productions of inflammatory factor including nitric oxide (NO), prostaglandin E2 (PGE₂), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), interleukin-1β (IL-1β) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. SY inhibited NO and PGE₂ production in a dose dependent manner and decreased the protein and mRNA expression of iNOS and COX-2. Also, SY decreased the mRNA expression of interleukin-6 (IL-6) and interleukin-1β (IL-1β). In conclusion, SY downregulated LPS-induced inflammatory factor productions, which could be a clinical basis for inflammatory diseases.

• 대한한방소아과학회지
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• 제23권3호
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• pp.233-240
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• 2009

Objectives To evaluate that Okwada affected which factors for treatment of lung fibrosis. Methods Bleomycin induced lung fibrosis model made in mice. After Okwada lyophilized, power sample obtained and melt in distilled water. Okwada solution administered mice through oral route on 21 days after bleomycin instillation and this procedure performed once a day for 7 days. We divided by three groups; normal (control), bleomycin induced lung fibrosis without treatment (experimental), bleomycin induced lung fibrosis with treatment (treatment). On six weeks after bleomycin instillation, mice sacrificed and removed lung. Weperformed Western blot analysis for TGF-beta, phosphodiesterase 5A, interleukin (4,5,13) and compared therapeutic effects of Okwada. Results On western blot analysis, all normal and experimental mice detected TGF-beta, phosphodiesterase 5A, interleukin 4,5,13. The amount of band of TGF-beta, phosphodiesterase 5A, interleukin 5 in experimental and treatment group was similar. However, interleukin 4,13 of treatment group decreased compared with experimental group. Conclusions Okwada would be effected the lung fibrosis through suppression of interleukin 4,13.

• 한방재활의학과학회지
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• 제24권3호
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• pp.99-110
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• 2014

Objectives The purpose of this study was to investigate the Anti-oxidation and anti-inflammatory effects of ethanol extract from asiasari radix (AR) on lipopolysaccharide (LPS)-induced in RAW 264.7 Cells Methods Anti-oxidative effects of AR were measured by scavenging activities of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and production of reactive oxygen species (ROS) in RAW 264.7 cells. Anti-inflammatory effects of AR were measured by mediators including nitric oxide(NO), interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necosis factors-${\alpha}$ (TNF-${\alpha}$) and iNOS, IL-$1{\beta}$, IL-6, TNF-${\alpha}$ mRNA expression in RAW 264.7 cells. Results Total phenolic content was expressed $28.77{\pm}1.67$. DPPH radical Scavenging was increased depend on AR ethanol extract. ABAT radical Scavenging was increased depend on AR ethanol extract. Production of ROS was significantly decreased by AR ethanol extract on concentration of 100 (${\mu}g/ml$). Production of NO was significantly decreased by AR ethanol extract on concentration of $100({\mu}g/ml)$. Production of IL-$1{\beta}$, interleukin-6 and TNF-${\alpha}$ were increased depend on AR ethanol extract. And Production of interleukin-6, TNF-${\alpha}$ were significantly decreased AR ethanol extract. iNOS, IL-$1{\beta}$, IL-6, TNF-${\alpha}$ mRNA expression of RAW 264.7 cells was increased depend on AR ethanol extract. Conclusions According to this study, AR ethanol extract has anti-oxidative and anti-inflammatoy effects.

• Journal of Life Science
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• 제9권1호
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• pp.58-63
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• 1999

Highly metastatic mouse T-lymphoma CS21 cells can grow in vitro when cocultured with CA12 lymph node stromal cells, but they undergo apoptotic cell death when separated from CA12 stromal cells. It has been found that cysteine and interleukin-7(IL-7) as antiapoptotic soluble factors that produced by CA12 stromal cells. In this study, we report that an ICE family protease is activated in CS21 cells when separated from CA12 stromal cells and cultured alone. Enzyme purification using an avidin affinity column revealed that the involved cysteine protease possessed caspase3-like death protease activity. In addition, when IL-7 was added to CS21 cell culture, the protease activity could not be detected during partial purification of the enzyme. Taken together, these results strongly suggest that the caspase3-like protease activation is suppressed by IL-7 as an antiapoptotic factor that leads to abrogation of apoptosis execution.

• Tuberculosis and Respiratory Diseases
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• 제49권5호
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• pp.568-575
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• 2000

연구배경 : Cytokine은 주요한 염증 매개 물질로서 질환에 따라 다른 양상을 보일 수 있어, 흉수를 일으키는 다양한 원인 질환의 감별에 이용되어 왔다. 이 중에서 악성 흉수, 결핵성 흉수, 부폐렴 삼출액 및 농흉에서 INF-${\gamma}$, IL-2R, IL-6, IL-1를 동사에 측정하여 비교한 연구는 많지 않아 본 연구를 시행하게 되었다. 방법 : 흉수를 주소로 내원한 환자 중에서 흉수 천자로 삼출액이 확인된 환자들을 대상으로 흉수를 채취하여, ELISA kit를 이용하여 INF-${\gamma}$, IL-2R, IL-6, IL-10 농도를 측정하였다. 결과 : 악성 흉수, 결핵성 흉수, 부폐렴 삼출액 및 농흉에서 흉수 내의 cytokine의 순서대로, INF-${\gamma}$는 $16.7{\pm}50$, $295.5{\pm}585.5$, $10.0{\pm}0$ pg/ml, IL-2R은 $3247.4{\pm}1713.3$, $7423.5{\pm}3752.8$, $3790.2{\pm}3201.1$ pg/ml, IL-6는 $600{\pm}12.8$, $556.4{\pm}161.7$, $514.4{\pm}224.8$ pg/ml, IL-10은 $28.2{\pm}55.5$, $11.3{\pm}11.7$, $98.4{\pm}141.7$ pg/ml로 각각 측정되어, 결핵성 흉수가 다른 원인의 흉수에 비해 IL-2R 농도가 의 있게 높았으며, IFN-${\gamma}$ 농도도 높은 경향을 나타내었으며, 부폐렴 삼출액에서는 IL-10농도가 다른 원인의 흉수에 비해 의미있게 높았다. 결론 : 결론적으로 흉수 내의 IL-2R농도 측정은 결핵성 흉수와 다른 원인의 흉수를 감별하는데 어느 정도 도움이 될 것으로 사료되며, IL-10 측정은 부폐렴 삼출액과 다른 원인의 흉수를 감별하는데 어느 정도 도움을 줄 것으로 생각된다.

• 한국해양바이오학회지
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• 제2권2호
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• pp.86-93
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• 2007

만성염증은 치주염, 대장염, 간염 및 관절염과 밀접한 관련성이 있다고 알려져 있다. 최근에 항염증제가 해양자원으로부터 개발되고 있다. 본 연구에서는 감태 (EC)가 항염증효과가 있다는 것이 발견되었다. 갈조류에 속하는 감태의 에탄올 추출물이 RAW 264.7 세포에서 tumor necrosis factor-${\alpha}$ interleukin-$1{\beta}$, interleukin-6 and prostaglandin $E_2$와 같은 염증매개체의 생성에 탁월한 효과를 나타내었다. 더욱이 reporter gene assay 및 western blot 분석 에 서 감태추출물은 대식세포에서 염증매개체의 발현을 조절하는 NF-${\kappa}B$ 전사인자의 불활성화를 통하여 항염증효과를 나타내었다. 뿐만 아니라 감태추출물은 만성염증에 중요한 역할을 하는 기질금속단백질의 활성을 억제 하였다. 이러한 결과는 갑태추출물이 만성염증을 억제하는데 잠재적으로 이용될 수 있다는 것을 암시하고 있다.

• 대한한방소아과학회지
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• 제14권2호
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• pp.61-84
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• 2000

The purpose of this study was to investigate the effects of Kami boatang(KBT) on the immune cells in Balb/c mice. KBT (500mg/kg) was administerd p.o. once a day for 7 days. KBT enhanced the proliferation of thymocytes and splenocytes. KBT enhanced the subpopulation of helper T(Th) cells, but did not affect the subpopulation of Thyl/B220 cells and Th/Tc cells in splenocytes. KBT enhanced the production of ${\gamma}$-interferon and interleukin-2 in thymocytes, but decreased the production of interleukin-4. KBT enhanced the production of ${\gamma}$-interferon, interleukin-2 and interleukin-4 in splenocytes and serum. KBT suppressed the production of nitric oxide, and enhanced the phagocytic activity in peritoneal macrophages. These results suggest that KBT has a potent activity on the immune response via the increase of the production of cytokines and phagocytic activity in vivo.

• 한국임상수의학회지
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• 제17권2호
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• pp.311-315
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• 2000

Recombinant interleukin-2 (IL-2) has been demonstated as an antineoplastic agent in mice and human, and the route of administration is important to IL-2-induced therapeutic responses. Therefore, the current experiment was undertaken to clarify the effect of IL-2 administration route on antitumor response against subcutaneous Meth-A tumor in mice. At the beginning of each experiment, normal BALB/c mice were injected subcutaneously with $5{\times}10^6$ Meth-A tumor cells. Beginning on day 7, experimental groups were treated with a 5-day course of IL-2 (intraperitoneal or subcutaneous injection of 30, 000 IU every 12 hours for 5 days). The result of this experiment revealed that Meth-A tumor grew progressively in control mice. Intraperitoneal IL-2 treatment decreased significantly tumor growth and prolonged survival, compared with control mice. Subcutaneous IL-2 treatment decreased significantly tumor growth until day 11 and tumor cells, grew progressively thereafter, but mice in this group survived longer than control mice.

• Journal of Periodontal and Implant Science
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• 제35권1호
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• pp.9-19
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• 2005

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sopharae Flos was sliced, and extracted with 100% methanol. LPS from p. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supematants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAw264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAw264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

• Biomolecules & Therapeutics
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• 제8권3호
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• pp.276-280
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• 2000

In order to identify the domains of the G$_{\alpha}$16 subunit G protein that are responsible for its activation by the Interleukin-8 receptor, a serious of chimeras between G$_{\alpha}$16 and G$_{\alpha}$11 were assessed for their abilities to be activated by these receptors. Co-expression of IL-8 receptor and chimeras in which the carboxyl-terminal regions of G$_{\alpha}$11 were replaced from 30 up to 156 amino acid residues with the corresponding regions of G$_{\alpha}$16 demonstrated that C-terminal 156 amino acid residues of the G$_{\alpha}$16 were not sufficient to confer IL-8 receptor interaction specificity. Testing of a reciprocal serious of chimeras composed of G$_{\alpha}$16 sequences at the amino terminus and G$_{\alpha}$11 sequences at the carboxyl terminals revealed that sequences extending from the amino tar- minus to amino acid 209 of G$_{\alpha}$16 were sufficient to 7ndow the chimera with 75-80% of interaction specificity for 7-8-induced activation. These results suggest th,.7t combined interactions of the C-terminal 30 amino acid residues and certain domains extending from the arts.ino terminus to amino acid 209 of Gal 6 protein may be involved in its couplings to IL-8 receptor.tain domains extending from the arts.ino terminus to amino acid 209 of Gal 6 protein may be involved in its couplings to IL-8 receptor.