• Molecules and Cells
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• v.40 no.9
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• pp.677-684
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• 2017

Postmenopausal atrophic vagina (PAV) is the thinning of the walls of the vagina and decreased lugae of the vagina. PAV is caused by decreased estrogen levels in postmenopausal women. However, the harmful effects of hormone replacement therapy (HRT) have resulted in considerable caution in its use. Various estrogen agonist treatment options are available. Vitamin D is influences the regulation of differentiation and proliferation of various cells, especially tissues lining stratified squamous epithelium, such as the vaginal epithelium. In this study, we hypothesized that vitamin D could provide an alternative and a safe treatment option for PAV by promoting the proliferation and differentiation of the vaginal epithelium. Thirty six patients were enrolled in this case-control study. Vitamin D associated proteins in a vitamin D and sex hormone treated vaginal epithelial cell line as well as normal and PAV tissues were measured. To confirm of cell-to-cell junction protein expression, cell line and tissue studies included RT-PCR, immunohistochemistry staining, and immunoblot analyses. The expression of cell-to-cell junction proteins was higher in women with symptoms of atrophic vagina tissue compared to women without the symptoms. Vitamin D stimulated the proliferation of the vaginal epithelium by activating p-RhoA and Erzin through the vitamin D receptor (VDR). The results suggest that vitamin D positively regulates cell-to-cell junction by increasing the VDR/p-RhoA/p-Ezrin pathway. This is the first study to verify the relationship of the expression of RhoA and Ezrin proteins in vaginal tissue of PAV.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.630-639
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• 2010

Immune response and yolk cholesterol are crucial factors for commercial chicken producers. The objectives of this study were to investigate the effect of selenium-enriched Japanese radish sprouts (Se-enriched JRS) and R. capsulatus synergistically on immune response and cholesterol in laying hens. A total of 50 laying hens (20-wk old) were assigned to 5 dietary treatment groups, and fed diets supplemented with 2.5 ${\mu}g/kg$, 5 ${\mu}g/kg$, 10 ${\mu}g/kg$ Se-enriched JRS and 5 ${\mu}g/kg$ Se-enriched JRS+R. capsulatus (0.02%). Egg production and yolk color were significantly improved by the supplementation of Se-enriched JRS+R. capsulatus in the layer diet (p<0.05). Compared to the control, serum cholesterol concentration and triglyceride levels were decreased by all the treatments (p<0.05). After 8-wk of the experiment, supplementation of 5 ${\mu}g/kg$, 10 ${\mu}g/kg$ and Se-enriched JRS+R. capsulatus significantly reduced yolk cholesterol and triglycerides, while the greatest reduction was observed when R. capsulatus was incorporated with Se-enriched JRS. Spleen, bursa and thymus weight were significantly increased by both the 5 ${\mu}g/kg$ and 10 ${\mu}g/kg$ Se-enriched JRS. Compared to the control, supplementation of 5 ${\mu}g/kg$ and 10 ${\mu}g/kg$ Se-enriched JRS significantly increased serum IgG and yolk IgY concentration and foot web index activity by Newcastle Disease Virus (p<0.05). After 4-wk and 8-wk of supplementation, the highest number of leukocytes was observed with Se-enriched JRS+R. capsulatus (p<0.05). The highest concentration of serum and yolk Se was found in Se-enriched JRS plus R. capsulatus treatment. Combined dietary supplementation of Se-enriched JRS and R. capsulatus might be beneficial for better health, disease protection and overall production performance.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.52-61
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• 2023

Fire blight, caused by Erwinia amylovora, is one of the major bacterial disease of apple and pear, causing enormous economic losses worldwide. Several control measures against E. amylovora have been reported till date, however, none of them have proved to be effective significantly against the pathogen. In this study, mechanisms of the copper-based control agents (CBCAs): copper oxychloride (COCHL), copper oxide (COX), copper hydroxide (CHY), copper sulfate basic (CSB), and tribasic copper sulfate (TCS) and their disease severity reduction efficacy against E. amylovora were analyzed. Bis-1,3-dibutylbarbituric acid trimethine oxonol, carboxyl fluorescein diacetate succinimidyl ester, and 5-cyano-2,3-ditolyl tetrazolium chloride staining were used to check the damage of membrane potential, cytoplasmic pH_in, and respiration of CBCAs-treated E. amylovora, respectively. High disturbance in the membrane potential of E. amylovora was found under COX and COCHL treatments. Similarly, higher significant changes in the inner cytoplasmic pH_in were observed under COX, COCHL, and TCS treatment. CHY and COCHL-treated E. amylovora showed a significant reduction in respiration. In vitro bioassay results revealed that CHY, CSB, and TCS at 2,000 ppm reduced the severity of fire blight both in pre- and post-treatment of CBCAs in immature apple fruits and seedlings. Overall, the most effective CBCAs against E. amylovora could be CHY at 2,000 ppm as its showed inhibition mechanisms and disease severity reduction.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.435-443
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• 2023

Global concern over climate change, driven by greenhouse gas emissions, has prompted widespread interest in sustainable solutions. In the agricultural sector, biochar has emerged as a focal point for mitigating these emissions. This study investigated the impact of continuous biochar application on CO₂ and N₂O emissions during the spring cabbage cultivation period. Greenhouse gas emissions in the biochar treatment groups (soils treated with 1, 3, and 5 tons/ha of rice husk biochar) were compared to those in the control group without biochar. During the spring cabbage cultivation period in 2022, the total CO₂ emissions were in the range of 71.6-119.0 g/m² day, and in 2023, with continuous biochar application, they were in the range of 71.6-102.1 g/m² day. The total emissions of N₂O in 2022 and 2023 were in the range of 11.7-23.7 and 7.8-19.9 g/m² day, respectively. Overall, greenhouse gas emissions decreased after biochar treatment, confirming the positive influence of biochar on mitigating greenhouse gas release from the soil. Nevertheless, further research over an extended period exceeding five years is deemed essential to delve into the specific mechanisms behind these observed changes and to assess the long-term sustainability of biochar's impact on greenhouse gas dynamics in agricultural settings.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.457-464
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• 2023

The rendering residue generated by rendering disposal, an eco-friendly livestock carcass disposal method, is a useful agricultural resource. Methods for recycling this are being actively researched, and this study investigated the impact of applying rendered residue directly to soil on crop productivity and the agricultural environment. The chemical properties of the rendering residue were examined. The pH, OM, T-N, T-P, CaO, K₂O, and MgO content values were 5.47%, 59.8%, 9.22%, 2.96%, 2.16%, 0.51% and 0.10%, respectively. Treatment conditions were divided into control, inorganic fertilizer, and rendering residue, and rendering residue corresponding to 50, 100, and 200% nitrogen content was applied based on the amount of inorganic fertilizer nitrogen input. Greenhouse gases and ammonia were collected during the cultivation period. Rendering residue increased both the yield and growth of peppers and was effective in improving nutrients such as pH and OM of the soil after harvest. However, compared to inorganic fertilizer treatment, it increased emissions of nitrous oxide and methane as well as ammonia. It is judged that the direct agricultural use of rendering residue is difficult, and a utilization method is needed.

• Journal of Periodontal and Implant Science
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• v.54 no.4
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• pp.265-279
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• 2024

Purpose: The aim of current study was to evaluate percentage root coverage (RC%) in isolated Miller class III/RT2 labial gingival recession (GR) associated with malaligned mandibular anteriors, using interdisciplinary periodontal-orthodontic treatment as compared to mucogingival surgery alone. Methods: Thirty-six systemically healthy patients having isolated Miller class III/RT2 GR with respect to malaligned mandibular anteriors, were randomly divided into test group: mucogingival surgery using subepithelial connective tissue graft followed by orthodontic treatment and control group: mucogingival surgery alone. Primary clinical parameters included (RC%), recession depth, keratinized tissue width, mid-labial clinical attachment level, interdental clinical attachment level (iCAL), periodontal phenotype (PP), gingival thickness (GT), root coverage esthetics score (RES) and hypersensitivity. Total duration of follow up was 12 months. Results: Mean RC% was significantly more achieved in test group (66.67%±40.82%) in comparison to control group (39.93%±31.41%) at the end of study (P=0.049). Further, complete root coverage was attained in 5/8 cases of test group versus 1/2 cases of control group after 3/12 months respectively. RES and hypersensitivity, showed statistically significant improvement after complete follow up period in both the groups. An ideal RES score of 10 was achieved in 4/7 cases of test group while in 1/2 cases of control group after 3/12 months respectively. Correlation analysis revealed significant negative correlation between RC% and iCAL. Correlation of RC% with GT and PP was non-significant. Conclusions: Interdisciplinary periodontal-orthodontic approach may be more beneficial in terms of achieving improved RC%, esthetic and resolution of hypersensitivity in the management of Miller class III/RT2 GR in malaligned mandibular anteriors.

• The korean journal of orthodontics
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• v.46 no.1
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• pp.42-54
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• 2016

The aim of this clinical report is to describe the complex treatment of an adult Class III malocclusion patient who was disappointed with the outcome of a previous oral rehabilitation. Interdisciplinary treatment planning was performed with a primary indication for implant removal because of marginal bone loss and gingival recession, followed by orthodontic and surgical procedures to correct the esthetics and skeletal malocclusion. The comprehensive treatment approach included: (1) implant removal in the area of the central incisors; (2) combined orthodontic decompensation with mesial displacement and forced extrusion of the lateral incisors; (3) extraction of the lateral incisors and placement of new implants corresponding to the central incisors, which received provisional crowns; (4) orthognathic surgery for maxillary advancement to improve occlusal and facial relationships; and finally, (5) orthodontic refinement followed by definitive prosthetic rehabilitation of the maxillary central incisors and reshaping of the adjacent teeth. At the three-year follow-up, clinical and radiographic examinations showed successful replacement of the central incisors and improved skeletal and esthetic appearances. Moreover, a Class II molar relationship was obtained with an ideal overbite, overjet, and intercuspation. In conclusion, we report the successful esthetic anterior rehabilitation of a complex case in which interdisciplinary treatment planning improved facial harmony, provided gingival architecture with sufficient width and thickness, and improved smile esthetics, resulting in enhanced patient comfort and satisfaction. This clinical case report might be useful to improve facial esthetics and occlusion in patients with dentoalveolar and skeletal defects.

• Textile Coloration and Finishing
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• v.24 no.4
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• pp.305-312
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• 2012

In this study, we investigated the effects of oxidative permanent hair coloring agents on the dyeability and the damage of human hair. p-phenylenediamine and toluene-2.5-diamine sulfate were used as a hair coloring agent precursers. The degree of dyeability was checked by the change of CIELAB $L^*$ value according to dyeing time. And the damage of hair was evaluated by the tensile strength and morphological change of hair in SEM. When the hair was dyed, the CIELAB $L^*$ value was decreased with dyeing time regardless of the type of precursers. But when the hair was dyed after nutritional treatment, the CIELAB $L^*$ value showed lower level. This means that the nutritional treatment covers the scale of hair and protects the hair from the chemicals.

• Journal of Interdisciplinary Genomics
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• v.4 no.2
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• pp.31-34
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• 2022

Background: Arteriovenous malformations (AVMs) are rare diseases comprising abnormally dilated arteries and veins with an absence of a capillary network. Since these diseases are intractable after diagnosis, various treatment strategies have been examined, with continuous efforts to identify target genes. Here, we report relevant new target genes selected via gene microarray. Methods: Endothelial cells were isolated from samples collected from three patients with AVM and three healthy individuals, followed by microarray analysis. Additionally, quantitative PCR was performed to select genes highly relevant to AVM. Results: In the vascular endothelial cells derived from the tissues of patients with AVM, the expression of ANGPT1, ANGPT2, DLL4, IL6, NRG1, TGFBR1, and VEGFA was typically higher compared to those derived from normal tissues. Conclusion: Seven candidate genes were selected to analyze the pathophysiological mechanism of AVM. These results may aid in future directions of diagnosis and treatment.

• Journal of Korean Neurosurgical Society
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• v.29 no.12
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• pp.1620-1627
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• 2000

Objectives : DAMA cases were analyzed to examine what the main casual factors of DAMA were and how to deal with these cases effectively in hospital with the DAMA interdisciplinary team including medical social worker whose role is to perform psycho-social assessment, family counsel, to evaluate family's DAMA need. Patients and Methods : The content analysis of medical record and social work record were reviewed in 37 cases referred by medical doctor to DAMA team. These cases were reported by patients' self discharge request or family's request for discharge from September 1998 to February 2000. The DAMA team consists of Assistant Director of Hospital as team leader, medical staff in-charge, social worker, QI nurse, other staff members who are not involved in direct treatment for patient, and administrative clerk. Results : The results of content analysis are as follows : 1) The most causal factors of DAMA consist of combination of more than 2 factors. 2) The major decision-maker is revealed to be son and daughter of patient. 3) In 59.4% of cases, family was not informed of patients' prognosis, alternatives, the consequence of DAMA at all. 4) In cases of DAMA report, the rapid intervention of social worker is carried out. Conclusion : In this study, we propose the interdisciplinary team approach to make decision legitimately and ethically for DAMA. The suggestions from this study are as follows : 1) To deal with DAMA case properly, the interdisciplinary team approach should be considered. 2) The criteria for DAMA case should be formed carefully. For the explicit selection of DAMA case, preliminary system for high-risk patient screening is recommended. 3) The medical social worker is available for the psycho-social problems of the patient once family members. For the effective family counselling, discharge planning and nursing home placement, the participation of medical social worker should be mandatory.