• Korean Journal of Microbiology
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• v.29 no.5
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• pp.270-277
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• 1991

A mutational alteration in the signal sequence of ribose-binding protein (RBP) of Escherichia coli, rbsB103, completely blocks the export of the protein to the periplasm. Intragenic suppressors for this mutation have been selected on minimal medium with ribose as a sole carbon source. Six suppressor mutations were characterized in detail and were found to have single amino acid wubstitution in the mature portion of RBP, which resulted in the mobility shift of the proteins on SDS polyacrylamide gel. Amino acid changes of these suppressors were localized in several peptides which are packed to form the N terminal domain of typical bilobate conformation of RBP. The involvement of SecB, a molecular chaperone, was investigated in the suppression of signal sequence mutation. Translocation efficency was found to be increased by the presence of SecB for all suppressors. It is likely that the folding characteristics of RBP altered by the suppressor mutations affect the affinity of interaction between SecB and RBP.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1103-1108
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• 2009

The human immunodeficiency virus (HIV)-l protein Tat acts as a transcription transactivator that stimulates expression of the infected viral genome. It is released from infected cells and can similarly affect neighboring cells. The nucleocapsid is an important protein that has a related significant role in early mRNA expression, and which contributes to the rapid viral replication that occurs during HIV-1 infection. To investigate the interaction between the Tat and nucleocapsid proteins, we utilized cDNA micro arrays using pTat and flag NC cotransfection in HEK 293T cells and reverse transcription-polymerase chain reaction to validate the micro array data. Four upregulated genes and nine downregulated genes were selected as candidate genes. Gene ontology analysis was conducted to define the biological process of the input genes. A proteomic approach using PathwayStudio determined the relationship between Tat and nucleocapsid; two automatically built pathways represented the interactions between the upregulated and downregulated genes. The results indicate that the up- and downregulated genes regulate HIV-1 replication and proliferation, and viral entry.

• Advances in concrete construction
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• v.6 no.3
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• pp.269-283
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• 2018

This study presents the effects of geometrical dimensions of concrete gravity dams on the seismic response considering different base width/dam height (L/H) ratios. In the study, a concrete gravity dam with the height of 200 m is selected and finite element models of the dam are constituted including five different L/H ratios such as 0.25, 0.5, 0.75, 1.00, 1.25. All dams are modeled in ANSYS software considering dam-reservoir-foundation interaction. 1989 Loma Prieta earthquake records are applied to models in upstream-downstream direction and linear time history analyses are performed. Dynamic equilibrium equations of motions obtained from the finite element models of the coupled systems are solved by using Newmark time integration algorithm. The seismic response of the models is evaluated from analyses presenting natural frequencies, mode shapes, displacements and principal stresses. The results show that the L/H ratios considerably affect the seismic response of gravity dams. Also, the model where L/H ratio is 1.00 has more desirable results and most appropriate representation of the seismic response of gravity dams.

• The Journal of Korea Robotics Society
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• v.4 no.4
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• pp.281-288
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• 2009

Nowadays, many robots have evolved to imitate human social skills such that sociable interaction with humans is possible. Socially interactive robots require abilities different from that of conventional robots. For instance, human-robot interactions are accompanied by emotion similar to human-human interactions. Robot emotional expression is thus very important for humans. This is particularly true for facial expressions, which play an important role in communication amongst other non-verbal forms. In this paper, we introduce a method of creating lifelike facial expressions in robots using variation of affect values which consist of the robot's emotions based on emotional boundaries. The proposed method was examined by experiments of two facial robot simulators.

• Journal of the Korean Society of Combustion
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• v.15 no.4
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• pp.1-8
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• 2010

Mild combustion is considered as a promising combustion technology for energy saving and low emission of combustion product gases. In this paper, the controllability of reaction region in mild combustion is examined by using co-axial air nozzle. For this purpose, numerical approach is carried out. Propane is considered for fuel and air is considered for oxidizer and the temperature of air is assumed 900K slightly higher than auto ignition temperature of propane. But unlike main air, the atmospheric condition of co-axial air is considered. Various cases are conducted to verify the characteristics of Co-Axial air burner configuration. The use of coaxial air can affect reaction region. These modification help the mixing between fuel and oxidizer. Then, reaction region is reduced compare to normal burner configuration. The enhancement of main air momentum also affects on temperature uniformity and reaction region. The eddy dissipation concept turbulence/chemistry interaction model is used with two step of global chemical reaction model.

• Korean Journal of Cognitive Science
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• v.2 no.1
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• pp.51-72
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• 1990

This study investigated the effects of macrostructure on discourse processing. In this study, three different story were employed to form macrostructures on different times, and reading time, sentence verification time and free recall rate were measured as dependent vari- ables. The results showed that forming the macrostructure influenced the reading and verification times and the recall rate. The results were interpreted to indicate that the macrostructure is important to comprehend and recall the stories, and that the story grammars, hierarchies of story schema and causal relation of sentences affect the comprehension and recall through their interaction in macrostructure.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.109-114
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• 1991

The depth and spread of root systems and the density of root branching affect the success and survival of plants subjected to drought. The type and size of root systems are controlled by heredity and soil conditions. There are also important interaction between roots and shoots; roots are dependent on shoots for carbohydrates, growth regulators, and certain vitamins, and shoots are dependent on roots for water, minerals, and certain growth regulators. Pinus thunbergii Parlatore had most of roots in the top 20cm of soil, and had little short of roots in the less than 80cm of soil. Average of root density was $270cm/cm^3$ in the upper soil(0~20cm). Root density of Pinus thunbergii Parlatore was similar to that of Cryptomeria japonica. There is no saying that Pinus thunbergii Parlatore has an advantage over Cryptomeria japonica in the drought. This data will be used to the root density values in the simulation model of black pine stands.

• Journal of the Korean Magnetic Resonance Society
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• v.13 no.1
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• pp.56-63
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• 2009

SWIRM domain, a core domain of SRG3 is well conserved in SW13, RSC8, and MOIRA family proteins. To understand structural basis for cellular functions of the SWIRM domain, we have initiated biochemical and structural studies on SWIRM domain and mutants using gelfiltration chromatography, circular dichroism and NMR spectroscopy. The structural properties of the mutant SWIRM domains (K34A and M75A) have been characterized, showing that the structures of both wild-type and mutant proteins are a-helical conformation. The data conclude that mutations at interaction sites of its binding partner protein do not affect its secondary and tertiary structure.

• 한국연소학회:학술대회논문집
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• 2012.11a
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• pp.229-231
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• 2012

A numerical analysis of a lean premixed combustor in a micro gas turbine was carried out to investigate the correlation between the turbulent mixing and emission characteristics on the combustor geometries. The interaction between the burners, by flow direction and momentum, significantly influenced on the turbulent mixing and combustion characteristics. The vortex which was generated by thermal expansion was observed during the combustion process, this was distinguished from the combustor geometries. The results showed that these characteristics can affect the NOx emission.

• Proceedings of the KSR Conference
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• 2003.10b
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• pp.442-447
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• 2003

The actual behavior of the railroad track structure is suspected to be a complex interaction between the vertical, lateral, longitudinal, and torsional behaviors. A FE program are developed in the present study to be used for extensive nonlinear analysis of the track structures subjected to thermal load. Using the rigorous study on the deformed shape of the rail and tie, and stress resultants, characteristics of the three dimensional behavior are investigated. It is found that the flexural rigidity of the tie and the rotational stiffness of pad-fastener can be affect the behavior of the track structure and the postbuckling behavior in each rail, except lateral behavior, is not same.