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Development of the Revegetation Technology for the Ecological Restoration of the Steep Rock-exposed Slopes by PEC Methods (PEC공법을 활용한 급경사 암비탈면의 생태복원녹화 기술개발에 관한 기초적 연구)

  • Kim, Nam-Chun;Jung, Ji-Jun;Lee, Byung-Jun;Kim, Sung-Ho;Kim, Yeon-Mee;Bae, Sun-Woo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.4
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    • pp.98-109
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    • 2004
  • This study was conducted to develop revegetation methods for the restoration of the steep slopes by recycling of bark compost and mushroom media. In general, bark compost and mushroom media can be used as soil media for the restoration works, because they can increase infiltration of rainfall and give enough porous to breathe and elongate for the root growth as well as environmental value. This experiment was carried out to know the effect of soil media composed by different ratio of mushroom media for the restoration of steep rock-exposed slopes, and to certificate how soil media(PEC) will be effective to germinate and grow for native plants. The main results of the study are summarized as follows; 1. In percent coverage, the soil media PEC1 is more valuable than PEC2. The seed mixtures recommended by Government of Transportation and Construction can be used at PEC1 and PEC2, but it will be more useful if the total amount of seed are reduced and seed mixtures are altered in a direction of native plants. 2. The soil media is under 20 mm tested by Yamanaka Hardiness Tester which is available for the seed germination and growth. 3. The surface cracks are not occurred in PEC1 and PEC2, but more than 30 cracks per 1 square meter are occurred at soil media which is constructed by normal soil-seed-fertilizer hydro-seeding methods. 4. The soil moisture contents are over 20 percent level during 15 day. Such moisture content in soil media will be effective for the plant growth. By using Terra-Control, PEC can maintain enough soil moisture. 5. The eroded soils from $60^{\circ}$ slopes by artificial rainfall with the intensity of 20 mm/hr in one day after seeding are estimated under 1%. By the results of erosion test, it comes to the conclusion that soil media of PEC can be adapted at steep rock exposed slopes.

Classification of Ground Subsidence Factors for Prediction of Ground Subsidence Risk (GSR) (굴착공사 중 지반함몰 위험예측을 위한 지반함몰인자 분류)

  • Park, Jin Young;Jang, Eugene;Kim, Hak Joon;Ihm, Myeong Hyeok
    • The Journal of Engineering Geology
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    • v.27 no.2
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    • pp.153-164
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    • 2017
  • The geological factors for causing ground subsidence are very diverse. It can be affected by any geological or extrinsic influences, and even within the same geological factor, the soil depression impact factor can be determined by different physical properties. As a result of reviewing a large number of papers and case histories, it can be seen that there are seven categories of ground subsidence factors. The depth and thickness of the overburden can affect the subsidence depending on the existence of the cavity, whereas the depth and orientation of the boundary between soil and rock are dominant factors in the ground composed of soil and rock. In case of soil layers, more various influencing factors exist such as type of soil, shear strength, relative density and degree of compaction, dry unit weight, water content, and liquid limit. The type of rock, distance from the main fracture and RQD can be influential factors in the bedrock. When approaching from the hydrogeological point of view, the rainfall intensity, the distance and the depth from the main channel, the coefficient of permeability and fluctuation of ground water level can influence to ground subsidence. It is also possible that the ground subsidence can be affected by external factors such as the depth of excavation and distance from the earth retaining wall, groundwater treatment methods at excavation work, and existence of artificial facilities such as sewer pipes. It is estimated that to evaluate the ground subsidence factor during the construction of underground structures in urban areas will be essential. It is expected that ground subsidence factors examined in this study will contribute for the reliable evaluation of the ground subsidence risk.

Endoplasmic Reticulum (ER) Stress and Apoptosis in Parthenogenetic Porcine Embryos following Different Combination of Activation Methods

  • Park, Hye-Bin;Park, Yeo-Reum;Lee, Hwa-Yeon;Bae, Hyo-Kyung;Lee, Seunghyung;Park, Choon-Keun;Yang, Boo-Keun;Cheong, Hee-Tae
    • Journal of Embryo Transfer
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    • v.32 no.1
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    • pp.25-31
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    • 2017
  • This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

Effects of Plant Spacing on Light Environment, Yield and Quality of Burley Tobacco (Burley종의 재식거잡가 광환경, 수량 및 품종에 미치는 영향)

  • 배성국;임해건
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.2
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    • pp.212-217
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    • 1981
  • The evaluate the effect of plant spacing on cured leaf of burley tobacco, the row spacings divided to 90, 105, 120cm and hill spacings to 30, 35, 40cm within each row. Growth amount per plant increased with thinner row and wider hill spacing in the same planting density. Relative light intensity increased with thinner row spacing in cutters and leaf and showed the positive correlation with quality. When the planting density was equal, the wider hill spacings, the more effective in utillization of solar radiation. The more plants per l0a were, the greater yield was obtained, and in the case of 3,200 plants per 10a (the most dense planting plot) was 267kg. But, quality, total-alkaloid and total-nitrogen content decreased with dense planting. Value per 10a was highest in the plots of 90 $\times$ 40cm and $105{\times}40cm$. In conclusion the optimum density level was 2,400 to 2,700 plants per 10a and spacing of tobacco either in 105 $\times$ 35 cm or 105 $\times$ 40cm seems to be most appropriate.

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Study on the Additive Effect of Epidermal Growth Factor (EGF) and Expression of EGF-Receptor (EGF-R) on IVM/IVF Bovine Embryo Development (체외 생산된 소 수정란의 발달에 있어서 EGF 첨가제 효과와 EGF-R 발현에 관한 연구)

  • 김은영;김묘경;엄상준;윤산현;박세필;정길생;임진호
    • Korean Journal of Animal Reproduction
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    • v.20 no.3
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    • pp.279-288
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    • 1996
  • The objective of this study was to determine the effect of EGF on the development of IVM/IVF bovine embryos and their ICM and TE cell number. In addition, we examined the combined effect of EGF and coculture to the bovine embryo development and the expression of EGF-R protein on bovine embryos by indirect immunofluorescence. The results obtained in these experiments were summarized as follows: When the IVM/IVF 4- to 8-cell embryos were treated at 0, 1, 10, 100 ng/ml of EGF, EGF treatment group showed improved development to blastocyst and increased pattern of ICM and TE cell number compared with control, although there is not significantly different. The stimulating effect of EGF (10 ng/ml) to the develop ment level of IVM/IVF bovine embryos significantly increased development rate to blastocyst after 8-cell stage (p<0.05), although there is no significant effect to the increase of ICM and TE cell numbers. Also, expression of EGF-R on the bovine embryonic stage by indirect immunofluor escence presents after 4-cell stage and the intensity of the EGF-R staining was variable with the development progression. On the other hand, embryos cultured in coculture group added either with or without EGF commonly indicated the significant difference in development rate to blastocyst and Total cell number compared with control. These results suggest that the a addition of EGF to the coculture may stimulate the coculture effect between IVM/IVF bovineembryos and cumulus cells. Therefore, EGF could promote preimplantation bovine embryo development by binding with expressed EGF~R after 4-cell stage, and stimulate the production of embryotrophic factors from the coculture environment. Also, the present study showed that there was no significant effect of EGF to the increase of ICM and TE cell number although the rate of blastocyst significantly increased when treated with EGF after 8-cell stage (p<0.05).

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Quantitative Analysis and Qualification of Amitrole Using LC/ESI-MS (LC/ESI-MS를 이용한 아미트롤의 정성확인 및 정량분석)

  • Park, Chan-Koo;Eo, Soo-Mi;Kim, Min-Young;Sohn, Jong-Ryeul;Mo, Sae-Young
    • Analytical Science and Technology
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    • v.17 no.2
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    • pp.117-129
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    • 2004
  • Amitrole in environment, difficult to be analyzed by GC or GC/MS due to high polarity and low volatility, was analyzed by LC/ESI/MS in the study. Maximum peak intensity of amitrole in LC/MS/ESI mass spectrum is m/z 85 of protonated molecular ion $(M+H)^+$ with 30V of cone voltage at SIR mode. It was confirmed that ratios between main ion of amitrole, 85 of protonated molecular ion, and m/z 58 fragmented ion of amitrole, had increased corresponding with the increment of cone voltage from 20V to 70V. The isotope molecular weight of amitrole was $86([M+H])^+$ at LC/MS analysis and the mass spectrum ratio between 85 mass and 86 mass was not different by the change of concentration but similar to theoretical ratio(less than 10% standard deviation).The linearity of standard calibration curve under same condition with sample treatment method had $y=1.09354e^6X+26947.2$ and $r^2=0.99$. Recovery rates in water and soil samples were 77.64~83.44% and 71.11~79.44%, respectively. Reliability of the analysis was performed with 5 repeated measurements at each level of standard concentration and the result showed that relative standard deviation was less than 10%; therefore, the extraction and analysis method in the study suggested that it would be reliable to measure amitrole in water and soil media.

THE EFFECT OF DIFFERENT CURING MODES ON COMPOSITE RESIN/DENTIN BOND STRENGTH IN CLASS ICAVITIES (1급 와동에서 상아질과 복합레진의 결합강도에 대한 중합방법의 효과)

  • Baek, Shin-Young;Cho, Young-Gon;Song, Byeong-Choon
    • Restorative Dentistry and Endodontics
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    • v.33 no.5
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    • pp.428-434
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    • 2008
  • The purpose of this study was to compare the microtensile bond strength in Class I cavities associated with different light curing modes of same light energy density. Occlusal enamel was removed to expose a flat dentin surface and twenty box-shaped Class I cavities were prepared in dentin. Single Bond (3M Dental product) was applied and Z 250 was inserted using bulk technique. The composite was light-cured using one of four techniques, pulse delay (PD group), soft-start (SS group), pulse cure (PC group) and standard continuous cure (CC group). The light-curing unit capable of adjusting time and intensity (VIP, Bisco Dental product) was selected and the light energy density for all curing modes was fixed at $16J/cm^2$. After storage for 24 hours, specimens were sectioned into beams with a rectangular cross-sectional area of approximately $1mm^2$ Microtensile bond strength $({\mu}TBS)$ test was per- formed using a univel·sal testing machine (EZ Test, Shimadzu Co.). The results were analyzed using oneway ANOVA and Tukey's test at significance level 0.05. The ${\mu}TBS$ of PD group and SS group was higher than that of PC group and CC group. Within the limitations of this in vitro study, modification of curing modes such as pulse delay and soft start polymerization can improve resin/dentin bond strength in Class I cavities by controlling polymerization velocity of composite resin.

The Effects of Pilates Exercise on Static and Dynamic Balance in the Elderly

  • Park, Ji-Hye;Lee, Joong-Sook;Yang, Jeong-Ok;Lee, Bom-Jin;Bae, Kang-Ho;Shin, Jin-Hyung
    • Korean Journal of Applied Biomechanics
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    • v.26 no.4
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    • pp.397-405
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    • 2016
  • Objective: Pilates is a low/mid-intensity exercise that can be easily performed by elderly individuals as it is an individual body-oriented exercise. It is also a cardio workout that can be performed anywhere to develop strength and flexibility. Therefore, we investigated the effects of 8 week Pilates program on the balancing ability of elderly individuals. Method: The research participants were selected from elderly residents in B city. Ten individuals voluntarily signed an agreement to undergo free measurements as well as to participate in the workout program. (Height: $157.1{\pm}11.9cm$, Weight: $61.7{\pm}8.0kg$). The Pilates exercise was performed 60 minutes a day, three times a week for a total of eight weeks. The measurement variables used to test balance were the vestibular test, 5 m habitual and maximum walk test and 3 m tandem walk test. A series of paired t-test were conducted using IBM SPSS Statistics 23.0 to analyze all the research data collected in order to determine the balance ability of the participants before and after the Pilates program. Additionally, the statistically significant level for all analysis was set to ${\alpha}=.05$. Results: In the vestibular test, some meaningful changes were observed in the length envelope area (ENV) while standing on one foot, but there were no significant differences in the ENV, rectangle(REC), root mean square, and total length. Results also revealed that statistically significant differences existed in the 5 m habitual and maximum walk test, as well as the 3 m tandem walk test. Conclusion: To summarize the findings, the 8 week Pilates program employed in this study significantly improved the dynamic balance of the elderly participants. Thus, elderly individuals that frequent perform Pilates are expected to enjoy positive benefits such as increased balance and fewer falling accidents.

The Effect of Direct Functional Magnetic Stimulation of the Lesion on Functional Motor Recovery in Spinal Cord Injured Rat (척수손상 흰 쥐의 운동기능 회복에 미치는 손상부위 직접자극을 통한 기능적 자기자극치료 효과)

  • Cho, Yun-Woo;Kim, Su-Jeong;Park, Hea-Woon;Seo, Jeong-Min;Hwang, Se-Jin;Jang, Sung-Ho;Lee, Dong-Gyu;Ahn, Sang-Ho
    • The Journal of Korean Physical Therapy
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    • v.23 no.1
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    • pp.53-58
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    • 2011
  • Purpose: The purpose of this study was to determine the effect of direct functional magnetic stimulation (FMS) of affected spinal cord on motor recovery following spinal cord injury in rats. Methods: After a contusion injury at the spinal level T9 using an NYU Impactor, functional magnetic stimulation was delivered by a magnetic stimulator through a round prototype coil (7 cm in diameter). Stimulation parameters were set as follows: repetition rate = 50 Hz (stimulus intensity 100% = 0.18 T), stimulation time = 20 min. Functional magnetic stimulation was administered twice a day, 5 days per week for 8 weeks starting 4 days after spinal cord injury. Functional magnetic stimulationwas delivered directly to the affected spinal cord. Outcomes of locomotor performance were assessed by the Basso Beattie Bresnahan (BBB) locomotor rating scale and by an inclined plane test weekly for 8 weeks. Results: In the BBB test, hindlimb motor function in the Functional magnetic stimulation group improved significantly more compared to the control group at 3, 4, 6, 7, and 8 weeks (p<0.05). In the inclined plane test, the angle of the plane in the functional magnetic stimulation group increased significantly more compared to the control group at 4, 5, 7, and 8 weeks (p<0.05). Conclusion: Our results demonstrate that direct Functional magnetic stimulation of the lesional site may have beneficial effects on motor improvement after spinal cord injury.

Development of a Traffic Condition Index (TCI) on Expressways (고속도로 소통상태지수 개발에 관한 연구)

  • Bok, Gi-Chan;Lee, Seung-Jun;Choe, Yun-Hyeok;Gang, Jeong-Gyu;Lee, Seung-Hwan
    • Journal of Korean Society of Transportation
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    • v.27 no.5
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    • pp.85-95
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    • 2009
  • Congestion on expressways is increasing in spite of continuous road construction. In enlargement of expressway capacity to lessen congestion, a long period is needed and in the case of traffic congestion, it would be impossible to avoid long periods of traffic congestion. So, it is necessary to cope with traffic congestion through continuous traffic condition monitoring, analysis of the causes of congestion and the development of alternatives before traffic conditions worsen. A congestion index that can express traffic operating conditions measurably is needed to monitor those conditions. Thus, in this research, a new congestion index, the Traffic Condition Index (TCI), is developed. TCI is able to evaluate roads that have different grades (or design speeds) and to judge traffic condition as good, fair and poor (congested). In addition, TCI has merits in that it can strengthen the function of existing Freeway Traffic Management Systems (FTMS) and can be applied to congestion management easily: TCI calculates congestion intensity and severity using data obtained from existing FTMS. In order to validate TCI, it was applied to the Kyungbu Expressway and the Seohaean Expressway. As a result, TCI shows a good performance in the aspect of applicability and ability of presentation of traffic conditions compared with travel speed and Travel Time Index (TTI).